| Definition | Yersinia pseudotuberculosis IP 31758, complete genome. |
|---|---|
| Accession | NC_009708 |
| Length | 4,723,306 |
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The map label for this gene is dkgB [H]
Identifier: 153949639
GI number: 153949639
Start: 1253586
End: 1254389
Strand: Direct
Name: dkgB [H]
Synonym: YpsIP31758_1048
Alternate gene names: 153949639
Gene position: 1253586-1254389 (Clockwise)
Preceding gene: 153950637
Following gene: 153949454
Centisome position: 26.54
GC content: 40.8
Gene sequence:
>804_bases ATGAGCATACCTGTATTCGGATTAGGAACTTTTCGCCTTCAAGATCAAATTGTTATTGATTCAGTGAGCCAAGCTCTGAC GTTGGGCTATCGGGCGATTGATACAGCACAAATATATGAAAATGAAGCGGCTGTTGGTCAGGCAATCCAAGAAAGTGGTA TTAATCGTGATGAACTATTTATTACTACTAAGATCTGGATTGCTAATTTATCAAAAGACAAATTAATTCCCAGCCTGCGA GAAAGCATACAGAAACTGAAAACTGACTATGTAGATTTAACACTGATTCATTGGCCATCACCTAATGATGAAGTTTCTGT TGCTGAATTTATGTCCGAGTTATTAAAAGCAAAAGGGCTAGGCTTAACTCGTCAAATTGGTATTTCTAACTTTACTATTG ATTTAATGAAGCAGGCGATTGCTGCCGTTGGTGCAGAAGAGATTGCAACTAATCAAATCGAGCTTTCTCCATTATTACAA AACCGTAATGTTGTTGATTTTGCCAAACAAAATGGCATTGCAGTGACTTCCTATATGACACTGGCTTATGGCAAGGCACT TGCAGAGCCAGTGATTAAAACGATTGCTGAGCAACATGGTGCGACCCCCGCACAAGTTATTTTGAGTTGGGCGATGCAAT TGGGTTATGGCGTTATTCCGTCATCGACTAAAGCTGCTAACTTGGCCAGCAACTTACTAGCACAGAAATTATGCCTGAAT GCAGCAGATATGGCGTTGATCGCCACATTGGATCGCAATGAGAGGTTGGTCAGCCCTGATGGTTTAGCACCTAAATGGGA TTAA
Upstream 100 bases:
>100_bases AGTTTTTCATCTGGTAGTTATTTAGAACATTCCACTGCCGATAAAAATTCGTTATGACTCGCAAGATAAAATGATTGTTA GCAAAAAATGAGGAACTATT
Downstream 100 bases:
>100_bases TAGCAGCCTAAGACACTGGACGAGCCACCTTACATTGAGGTGGTTTTTTATTTTGGTGCATCCTGACGGGGCTGAAACAG GCTCGGTGAAAAGTGTGCGG
Product: 2,5-diketo-D-gluconate reductase B
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 267; Mature: 266
Protein sequence:
>267_residues MSIPVFGLGTFRLQDQIVIDSVSQALTLGYRAIDTAQIYENEAAVGQAIQESGINRDELFITTKIWIANLSKDKLIPSLR ESIQKLKTDYVDLTLIHWPSPNDEVSVAEFMSELLKAKGLGLTRQIGISNFTIDLMKQAIAAVGAEEIATNQIELSPLLQ NRNVVDFAKQNGIAVTSYMTLAYGKALAEPVIKTIAEQHGATPAQVILSWAMQLGYGVIPSSTKAANLASNLLAQKLCLN AADMALIATLDRNERLVSPDGLAPKWD
Sequences:
>Translated_267_residues MSIPVFGLGTFRLQDQIVIDSVSQALTLGYRAIDTAQIYENEAAVGQAIQESGINRDELFITTKIWIANLSKDKLIPSLR ESIQKLKTDYVDLTLIHWPSPNDEVSVAEFMSELLKAKGLGLTRQIGISNFTIDLMKQAIAAVGAEEIATNQIELSPLLQ NRNVVDFAKQNGIAVTSYMTLAYGKALAEPVIKTIAEQHGATPAQVILSWAMQLGYGVIPSSTKAANLASNLLAQKLCLN AADMALIATLDRNERLVSPDGLAPKWD >Mature_266_residues SIPVFGLGTFRLQDQIVIDSVSQALTLGYRAIDTAQIYENEAAVGQAIQESGINRDELFITTKIWIANLSKDKLIPSLRE SIQKLKTDYVDLTLIHWPSPNDEVSVAEFMSELLKAKGLGLTRQIGISNFTIDLMKQAIAAVGAEEIATNQIELSPLLQN RNVVDFAKQNGIAVTSYMTLAYGKALAEPVIKTIAEQHGATPAQVILSWAMQLGYGVIPSSTKAANLASNLLAQKLCLNA ADMALIATLDRNERLVSPDGLAPKWD
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI93277124, Length=300, Percent_Identity=31, Blast_Score=143, Evalue=2e-34, Organism=Homo sapiens, GI223468663, Length=289, Percent_Identity=31.1418685121107, Blast_Score=139, Evalue=3e-33, Organism=Homo sapiens, GI310109922, Length=280, Percent_Identity=30, Blast_Score=137, Evalue=1e-32, Organism=Homo sapiens, GI24497577, Length=289, Percent_Identity=29.0657439446367, Blast_Score=137, Evalue=1e-32, Organism=Homo sapiens, GI5174391, Length=289, Percent_Identity=29.0657439446367, Blast_Score=137, Evalue=1e-32, Organism=Homo sapiens, GI310109920, Length=297, Percent_Identity=28.6195286195286, Blast_Score=135, Evalue=4e-32, Organism=Homo sapiens, GI45446745, Length=297, Percent_Identity=28.6195286195286, Blast_Score=135, Evalue=5e-32, Organism=Homo sapiens, GI4503285, Length=297, Percent_Identity=28.6195286195286, Blast_Score=135, Evalue=5e-32, Organism=Homo sapiens, GI5453543, Length=297, Percent_Identity=28.2828282828283, Blast_Score=134, Evalue=1e-31, Organism=Homo sapiens, GI24497585, Length=296, Percent_Identity=28.3783783783784, Blast_Score=132, Evalue=3e-31, Organism=Homo sapiens, GI24497583, Length=297, Percent_Identity=28.956228956229, Blast_Score=131, Evalue=6e-31, Organism=Homo sapiens, GI4502049, Length=291, Percent_Identity=32.9896907216495, Blast_Score=127, Evalue=1e-29, Organism=Homo sapiens, GI291291012, Length=273, Percent_Identity=30.4029304029304, Blast_Score=126, Evalue=2e-29, Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=27.6450511945392, Blast_Score=121, Evalue=8e-28, Organism=Homo sapiens, GI300116273, Length=268, Percent_Identity=27.6119402985075, Blast_Score=112, Evalue=3e-25, Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=27.3381294964029, Blast_Score=105, Evalue=3e-23, Organism=Homo sapiens, GI310109926, Length=114, Percent_Identity=35.0877192982456, Blast_Score=76, Evalue=4e-14, Organism=Homo sapiens, GI207028673, Length=115, Percent_Identity=36.5217391304348, Blast_Score=76, Evalue=4e-14, Organism=Homo sapiens, GI310109924, Length=160, Percent_Identity=28.125, Blast_Score=74, Evalue=1e-13, Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=75.6554307116105, Blast_Score=406, Evalue=1e-115, Organism=Escherichia coli, GI87082198, Length=262, Percent_Identity=37.4045801526718, Blast_Score=174, Evalue=5e-45, Organism=Escherichia coli, GI1788081, Length=263, Percent_Identity=26.9961977186312, Blast_Score=93, Evalue=2e-20, Organism=Escherichia coli, GI48994888, Length=250, Percent_Identity=26, Blast_Score=72, Evalue=3e-14, Organism=Escherichia coli, GI1787674, Length=273, Percent_Identity=27.8388278388278, Blast_Score=69, Evalue=3e-13, Organism=Escherichia coli, GI1788070, Length=216, Percent_Identity=26.8518518518519, Blast_Score=63, Evalue=2e-11, Organism=Caenorhabditis elegans, GI17550248, Length=290, Percent_Identity=33.7931034482759, Blast_Score=150, Evalue=6e-37, Organism=Caenorhabditis elegans, GI17537077, Length=288, Percent_Identity=32.2916666666667, Blast_Score=136, Evalue=1e-32, Organism=Caenorhabditis elegans, GI17537075, Length=290, Percent_Identity=32.4137931034483, Blast_Score=135, Evalue=2e-32, Organism=Caenorhabditis elegans, GI17561298, Length=275, Percent_Identity=28.3636363636364, Blast_Score=133, Evalue=8e-32, Organism=Caenorhabditis elegans, GI17552492, Length=262, Percent_Identity=30.5343511450382, Blast_Score=129, Evalue=1e-30, Organism=Caenorhabditis elegans, GI17561300, Length=274, Percent_Identity=29.1970802919708, Blast_Score=124, Evalue=5e-29, Organism=Caenorhabditis elegans, GI17566692, Length=288, Percent_Identity=30.5555555555556, Blast_Score=124, Evalue=5e-29, Organism=Caenorhabditis elegans, GI71998625, Length=265, Percent_Identity=29.0566037735849, Blast_Score=124, Evalue=6e-29, Organism=Caenorhabditis elegans, GI17537079, Length=295, Percent_Identity=27.1186440677966, Blast_Score=121, Evalue=3e-28, Organism=Caenorhabditis elegans, GI17564128, Length=297, Percent_Identity=28.2828282828283, Blast_Score=113, Evalue=1e-25, Organism=Caenorhabditis elegans, GI17562292, Length=269, Percent_Identity=29.368029739777, Blast_Score=112, Evalue=2e-25, Organism=Caenorhabditis elegans, GI17538386, Length=265, Percent_Identity=28.6792452830189, Blast_Score=111, Evalue=4e-25, Organism=Saccharomyces cerevisiae, GI6324694, Length=292, Percent_Identity=34.5890410958904, Blast_Score=165, Evalue=7e-42, Organism=Saccharomyces cerevisiae, GI6320576, Length=282, Percent_Identity=36.8794326241135, Blast_Score=164, Evalue=2e-41, Organism=Saccharomyces cerevisiae, GI6321896, Length=315, Percent_Identity=28.5714285714286, Blast_Score=125, Evalue=7e-30, Organism=Saccharomyces cerevisiae, GI6322556, Length=262, Percent_Identity=27.0992366412214, Blast_Score=116, Evalue=3e-27, Organism=Saccharomyces cerevisiae, GI6319625, Length=269, Percent_Identity=31.2267657992565, Blast_Score=111, Evalue=9e-26, Organism=Saccharomyces cerevisiae, GI6320079, Length=242, Percent_Identity=29.3388429752066, Blast_Score=88, Evalue=2e-18, Organism=Drosophila melanogaster, GI24644950, Length=298, Percent_Identity=33.5570469798658, Blast_Score=154, Evalue=6e-38, Organism=Drosophila melanogaster, GI221378297, Length=315, Percent_Identity=31.4285714285714, Blast_Score=144, Evalue=6e-35, Organism=Drosophila melanogaster, GI24663317, Length=289, Percent_Identity=30.1038062283737, Blast_Score=137, Evalue=7e-33, Organism=Drosophila melanogaster, GI24662789, Length=294, Percent_Identity=31.9727891156463, Blast_Score=137, Evalue=9e-33, Organism=Drosophila melanogaster, GI24657054, Length=280, Percent_Identity=31.4285714285714, Blast_Score=136, Evalue=1e-32, Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=31.1188811188811, Blast_Score=132, Evalue=2e-31, Organism=Drosophila melanogaster, GI21356425, Length=281, Percent_Identity=29.1814946619217, Blast_Score=129, Evalue=3e-30, Organism=Drosophila melanogaster, GI45553081, Length=284, Percent_Identity=27.112676056338, Blast_Score=115, Evalue=4e-26, Organism=Drosophila melanogaster, GI24662785, Length=290, Percent_Identity=32.4137931034483, Blast_Score=114, Evalue=6e-26, Organism=Drosophila melanogaster, GI24662781, Length=290, Percent_Identity=31.3793103448276, Blast_Score=110, Evalue=1e-24, Organism=Drosophila melanogaster, GI281366140, Length=258, Percent_Identity=26.3565891472868, Blast_Score=97, Evalue=1e-20,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 29010; Mature: 28879
Theoretical pI: Translated: 4.79; Mature: 4.79
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 2.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSIPVFGLGTFRLQDQIVIDSVSQALTLGYRAIDTAQIYENEAAVGQAIQESGINRDELF CCCCEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEE ITTKIWIANLSKDKLIPSLRESIQKLKTDYVDLTLIHWPSPNDEVSVAEFMSELLKAKGL EEEEEEEEECCHHHCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCC GLTRQIGISNFTIDLMKQAIAAVGAEEIATNQIELSPLLQNRNVVDFAKQNGIAVTSYMT CCEEECCCCHHHHHHHHHHHHHHCHHHHHCCCEEEHHHHCCCCCHHHHHCCCEEEHHHHH LAYGKALAEPVIKTIAEQHGATPAQVILSWAMQLGYGVIPSSTKAANLASNLLAQKLCLN HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC AADMALIATLDRNERLVSPDGLAPKWD HHHHHEEEEECCCCEEECCCCCCCCCC >Mature Secondary Structure SIPVFGLGTFRLQDQIVIDSVSQALTLGYRAIDTAQIYENEAAVGQAIQESGINRDELF CCCEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEE ITTKIWIANLSKDKLIPSLRESIQKLKTDYVDLTLIHWPSPNDEVSVAEFMSELLKAKGL EEEEEEEEECCHHHCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCC GLTRQIGISNFTIDLMKQAIAAVGAEEIATNQIELSPLLQNRNVVDFAKQNGIAVTSYMT CCEEECCCCHHHHHHHHHHHHHHCHHHHHCCCEEEHHHHCCCCCHHHHHCCCEEEHHHHH LAYGKALAEPVIKTIAEQHGATPAQVILSWAMQLGYGVIPSSTKAANLASNLLAQKLCLN HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC AADMALIATLDRNERLVSPDGLAPKWD HHHHHEEEEECCCCEEECCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11586360; 12142430 [H]