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The map label for this gene is iolD [H]

Identifier: 153949603

GI number: 153949603

Start: 3346965

End: 3348911

Strand: Reverse

Name: iolD [H]

Synonym: YpsIP31758_2976

Alternate gene names: 153949603

Gene position: 3348911-3346965 (Counterclockwise)

Preceding gene: 153950058

Following gene: 153950675

Centisome position: 70.9

GC content: 56.7

Gene sequence:

>1947_bases
ATGAGCACCGTAAACATGGCGATGAAAACAGTACGGATGACCATGGCACAGGCGCTGGTCAGGTTTCTTAATCAGCAATA
TGTGGCCATTGATGGTCAGGAGGTCGCATTTGTCCACGGCGTGATGACGATTTTCGGGCACGGCAATGTACTCGGTATCG
GCCAGGCTCTGGAAGAAAATCCGGGCCATTTACAGGTGCATCAGGGCTGTAACGAGCAGGGCATGGCGCATATGGCGATT
GGTTTTGCGAAACAGTATCGCCGTCAGCGTATCTATGCCGTCAGTTCGTCTATCGGGCCGGGAGCCGCCAATATGGTCAC
CGCAGCGGCCACCGCCACGGCAAACCGCATTCCGCTGCTGCTATTGCCGGGGGATATCTACGCCAGCCGTCAGCCTGACC
CGGTGTTACAGCAGATCGAGCAATATCACGACCTTAGTATTAGCACTAACGATTGCTTCCGCCCGGTATCACGTTACTGG
GATCGCATTAATCGCCCTGAGCAATTGATGAGCGCCATGCTGAGTGCTATGCGAGTACTGACTGACCCGGCGGATACCGG
TGCGGTGACCCTTTGTCTGCCTCAGGATGTGCAGGGCGAAGCGTGGGATTACCCGGCGAGCTTCTTTGAGCGCCGGGTGC
ATCGTATCGAGCGCCGTCCACCGGATGCGCGCAGGCTTTCTGATGCGCTCAGCCTGATCGCCCGTAAACGGCGTCCGCTG
GTGGTGTGTGGCGGCGGCGTGCGCTATTCGGGGGCGCATGAGGCGTTTCGTGCATTTGTGGAAAACGCTCAATTACCCTT
TACTGAAACCCAAGCGGGCAAAGGGGCGCTGCCAAGTGATCATCCCCTCAACTTGGGGGGAATTGGTGTCACTGGCGGGT
TGGCAGCTAACCGGTTAGCTCCACAGGCTGATCTGGTGATCGGCATTGGTACCCGGCTCACCGATTTCACTACGGGTTCC
AAAGCACTATTTTCTCACCCGGAGGTGGCGTTTTTGCTGCTTAACGTGGCGGAATTCGATGCACTTAAACTGGATGCCAC
GGCCCTGATTGCCGATGCGCGGGTGGGGCTTGAGGCATTAACCGAAGGGCTGGCAGGTTATCGCACTGCTTGGCAGGGCG
ATATCATTGAGGCCAAAGCAGCGTGGTGCGACGAATGTCAACGGCTTTGGTCACGCACCTGGCGGCCTGATGAGGTGCCA
GAGGTGGCCGGTCATCTTGATGATTCGCTACAAGAATACGCCGAGGTGCTCAATACCACACTGACCCAGACTCGGGTACT
TGGGCTTATCAACCAGCATATTGAAGACGATGCCATTGTCGTGGGGGCGGCGGGGTCGCTGCCGGGGGATTTACAGCGTC
TATGGCAGGTAAAAACGGCAGACACCTATCATCTTGAATACGGTTATTCATGCATGGGCTATGAAATCGCTGCCGCCATC
GGGGCGAAAATTGCGATGCCACATCAACCGGTGTACGCCATGGTGGGTGATGGCTCCTATATGATGCTGCATTCCGAGCT
GCACACGGCGGTACAGGAGGGGCTAAAAATTACCGTCCTACTGTTTGATAACGCCGGATTCGGCTGTATCAACAACCTGC
AAATGGGCCACGGGATGGGCAGTTTTTGTACCGAGAGCCGCCACCGTAACCACGAGTCGGGGCAACTCAATGGCCCATTG
ATTCGCGTTGATTTCGCCCAAAACGCCGAAAGTTATGGCTGCCGCGCCTGGCGGGTGCACGATGAACCAAGCCTGCTAGC
GGCATTGGCAGCATCCCGCGATCATCCGGGGCCGGTGCTACTGGATATTAAAGTCTTGCCGAAAAGCATGACGCACGATT
ATGCGTCTTGGTGGCGTACTGGCGATGCGCAAATCGCCCGTTCACCCGCGGTACAGCAGGCTGCAGAGCGAACCCAGATG
AAGGTAAAACGTGCCCGTCAGTACTAA

Upstream 100 bases:

>100_bases
TACACGCGGATGAAAACTATCACTGCCCGCTGGCCAGAAGCACAGCATGATCAGGGCGCGGCATTCTCTATGCCGACACT
CGGTTGAAGGGAGAGGCCGC

Downstream 100 bases:

>100_bases
CCCTCCGTTATCCTGCCTGTTCATCCGTCGTTGGCTGCAAAAAATGGGGTTAGTTTTAGAAAATGTTGTGACTGTGATAG
CAAAATTAACTCACTTTATT

Product: thiamine pyrophosphate-dependent enzyme

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 648; Mature: 647

Protein sequence:

>648_residues
MSTVNMAMKTVRMTMAQALVRFLNQQYVAIDGQEVAFVHGVMTIFGHGNVLGIGQALEENPGHLQVHQGCNEQGMAHMAI
GFAKQYRRQRIYAVSSSIGPGAANMVTAAATATANRIPLLLLPGDIYASRQPDPVLQQIEQYHDLSISTNDCFRPVSRYW
DRINRPEQLMSAMLSAMRVLTDPADTGAVTLCLPQDVQGEAWDYPASFFERRVHRIERRPPDARRLSDALSLIARKRRPL
VVCGGGVRYSGAHEAFRAFVENAQLPFTETQAGKGALPSDHPLNLGGIGVTGGLAANRLAPQADLVIGIGTRLTDFTTGS
KALFSHPEVAFLLLNVAEFDALKLDATALIADARVGLEALTEGLAGYRTAWQGDIIEAKAAWCDECQRLWSRTWRPDEVP
EVAGHLDDSLQEYAEVLNTTLTQTRVLGLINQHIEDDAIVVGAAGSLPGDLQRLWQVKTADTYHLEYGYSCMGYEIAAAI
GAKIAMPHQPVYAMVGDGSYMMLHSELHTAVQEGLKITVLLFDNAGFGCINNLQMGHGMGSFCTESRHRNHESGQLNGPL
IRVDFAQNAESYGCRAWRVHDEPSLLAALAASRDHPGPVLLDIKVLPKSMTHDYASWWRTGDAQIARSPAVQQAAERTQM
KVKRARQY

Sequences:

>Translated_648_residues
MSTVNMAMKTVRMTMAQALVRFLNQQYVAIDGQEVAFVHGVMTIFGHGNVLGIGQALEENPGHLQVHQGCNEQGMAHMAI
GFAKQYRRQRIYAVSSSIGPGAANMVTAAATATANRIPLLLLPGDIYASRQPDPVLQQIEQYHDLSISTNDCFRPVSRYW
DRINRPEQLMSAMLSAMRVLTDPADTGAVTLCLPQDVQGEAWDYPASFFERRVHRIERRPPDARRLSDALSLIARKRRPL
VVCGGGVRYSGAHEAFRAFVENAQLPFTETQAGKGALPSDHPLNLGGIGVTGGLAANRLAPQADLVIGIGTRLTDFTTGS
KALFSHPEVAFLLLNVAEFDALKLDATALIADARVGLEALTEGLAGYRTAWQGDIIEAKAAWCDECQRLWSRTWRPDEVP
EVAGHLDDSLQEYAEVLNTTLTQTRVLGLINQHIEDDAIVVGAAGSLPGDLQRLWQVKTADTYHLEYGYSCMGYEIAAAI
GAKIAMPHQPVYAMVGDGSYMMLHSELHTAVQEGLKITVLLFDNAGFGCINNLQMGHGMGSFCTESRHRNHESGQLNGPL
IRVDFAQNAESYGCRAWRVHDEPSLLAALAASRDHPGPVLLDIKVLPKSMTHDYASWWRTGDAQIARSPAVQQAAERTQM
KVKRARQY
>Mature_647_residues
STVNMAMKTVRMTMAQALVRFLNQQYVAIDGQEVAFVHGVMTIFGHGNVLGIGQALEENPGHLQVHQGCNEQGMAHMAIG
FAKQYRRQRIYAVSSSIGPGAANMVTAAATATANRIPLLLLPGDIYASRQPDPVLQQIEQYHDLSISTNDCFRPVSRYWD
RINRPEQLMSAMLSAMRVLTDPADTGAVTLCLPQDVQGEAWDYPASFFERRVHRIERRPPDARRLSDALSLIARKRRPLV
VCGGGVRYSGAHEAFRAFVENAQLPFTETQAGKGALPSDHPLNLGGIGVTGGLAANRLAPQADLVIGIGTRLTDFTTGSK
ALFSHPEVAFLLLNVAEFDALKLDATALIADARVGLEALTEGLAGYRTAWQGDIIEAKAAWCDECQRLWSRTWRPDEVPE
VAGHLDDSLQEYAEVLNTTLTQTRVLGLINQHIEDDAIVVGAAGSLPGDLQRLWQVKTADTYHLEYGYSCMGYEIAAAIG
AKIAMPHQPVYAMVGDGSYMMLHSELHTAVQEGLKITVLLFDNAGFGCINNLQMGHGMGSFCTESRHRNHESGQLNGPLI
RVDFAQNAESYGCRAWRVHDEPSLLAALAASRDHPGPVLLDIKVLPKSMTHDYASWWRTGDAQIARSPAVQQAAERTQMK
VKRARQY

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=563, Percent_Identity=22.202486678508, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI21361361, Length=484, Percent_Identity=23.1404958677686, Blast_Score=80, Evalue=6e-15,
Organism=Escherichia coli, GI1790104, Length=621, Percent_Identity=26.8921095008052, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI87081685, Length=548, Percent_Identity=27.1897810218978, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1786717, Length=547, Percent_Identity=24.8628884826325, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1788716, Length=438, Percent_Identity=23.7442922374429, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=653, Percent_Identity=25.2679938744257, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19922626, Length=541, Percent_Identity=23.2902033271719, Blast_Score=85, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 71018; Mature: 70887

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVNMAMKTVRMTMAQALVRFLNQQYVAIDGQEVAFVHGVMTIFGHGNVLGIGQALEEN
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCEEECCHHHHCC
PGHLQVHQGCNEQGMAHMAIGFAKQYRRQRIYAVSSSIGPGAANMVTAAATATANRIPLL
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCCEEE
LLPGDIYASRQPDPVLQQIEQYHDLSISTNDCFRPVSRYWDRINRPEQLMSAMLSAMRVL
EECCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
TDPADTGAVTLCLPQDVQGEAWDYPASFFERRVHRIERRPPDARRLSDALSLIARKRRPL
CCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCE
VVCGGGVRYSGAHEAFRAFVENAQLPFTETQAGKGALPSDHPLNLGGIGVTGGLAANRLA
EEECCCCEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
PQADLVIGIGTRLTDFTTGSKALFSHPEVAFLLLNVAEFDALKLDATALIADARVGLEAL
CCCCEEEECCCCEEECCCCCHHHHCCCCHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHH
TEGLAGYRTAWQGDIIEAKAAWCDECQRLWSRTWRPDEVPEVAGHLDDSLQEYAEVLNTT
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LTQTRVLGLINQHIEDDAIVVGAAGSLPGDLQRLWQVKTADTYHLEYGYSCMGYEIAAAI
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
GAKIAMPHQPVYAMVGDGSYMMLHSELHTAVQEGLKITVLLFDNAGFGCINNLQMGHGMG
CCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCC
SFCTESRHRNHESGQLNGPLIRVDFAQNAESYGCRAWRVHDEPSLLAALAASRDHPGPVL
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCEE
LDIKVLPKSMTHDYASWWRTGDAQIARSPAVQQAAERTQMKVKRARQY
EEEEECCCHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STVNMAMKTVRMTMAQALVRFLNQQYVAIDGQEVAFVHGVMTIFGHGNVLGIGQALEEN
CHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCEEECCHHHHCC
PGHLQVHQGCNEQGMAHMAIGFAKQYRRQRIYAVSSSIGPGAANMVTAAATATANRIPLL
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCCEEE
LLPGDIYASRQPDPVLQQIEQYHDLSISTNDCFRPVSRYWDRINRPEQLMSAMLSAMRVL
EECCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
TDPADTGAVTLCLPQDVQGEAWDYPASFFERRVHRIERRPPDARRLSDALSLIARKRRPL
CCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCE
VVCGGGVRYSGAHEAFRAFVENAQLPFTETQAGKGALPSDHPLNLGGIGVTGGLAANRLA
EEECCCCEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
PQADLVIGIGTRLTDFTTGSKALFSHPEVAFLLLNVAEFDALKLDATALIADARVGLEAL
CCCCEEEECCCCEEECCCCCHHHHCCCCHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHH
TEGLAGYRTAWQGDIIEAKAAWCDECQRLWSRTWRPDEVPEVAGHLDDSLQEYAEVLNTT
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LTQTRVLGLINQHIEDDAIVVGAAGSLPGDLQRLWQVKTADTYHLEYGYSCMGYEIAAAI
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
GAKIAMPHQPVYAMVGDGSYMMLHSELHTAVQEGLKITVLLFDNAGFGCINNLQMGHGMG
CCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCC
SFCTESRHRNHESGQLNGPLIRVDFAQNAESYGCRAWRVHDEPSLLAALAASRDHPGPVL
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCEE
LDIKVLPKSMTHDYASWWRTGDAQIARSPAVQQAAERTQMKVKRARQY
EEEEECCCHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA