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The map label for this gene is tesA [H]

Identifier: 153949498

GI number: 153949498

Start: 3400689

End: 3401324

Strand: Direct

Name: tesA [H]

Synonym: YpsIP31758_3021

Alternate gene names: 153949498

Gene position: 3400689-3401324 (Clockwise)

Preceding gene: 153948176

Following gene: 153947795

Centisome position: 72.0

GC content: 51.73

Gene sequence:

>636_bases
ATGAACTTCAAGAACGTTTTCCGCTGGCATCTTCCCTTCCTTTTACTGTTGGGATTATTCAGTTTGCGTGCCGCCGCAAC
CGATACACTATTGATTCTCGGCGACAGTTTAAGCGCCGGTTACCGCCTGCCTATTGCACAGGCATGGCCTTCGCTATTGG
ATAAAAAATGGCAAGCCACGCCTTCGTTACCGAAGGTGGTCAATGCCAGTATCAGTGGAGATACCGCCGCGCAAGGGCTA
GCGCGCTTACCCGCCCTACTCAAGCAACATCAGCCACGCTGGGTGCTGATTGAACTGGGTGCCAATGACGCGCTGCGCGG
GTTTCCGACGCAGGACATTCAACGGGATCTGAGCGAAATTATCAATCAGATAACCGCAGCGAAAGCCCAGCCATTATTGA
TGCAAATCCGTATTCCCCCCAACTATGGCCGCCGTTATACTGATGCTTTCACCGCTATCTATCCACAACTGGCGCAACAG
TTCGACATCCCCCTACTCCCTTTTTTTATGGAACAGGTTGCCGTTAAGCCAGAGTGGATGCAGGATGATGGTTTACACCC
TAACGGCGATGCCCAACCGTTTATCGCGGATTGGATGGCGCAACAACTCAAGCCGTTAGTTGTTGATCCGAAATAA

Upstream 100 bases:

>100_bases
CGCCAATCAGCGCAATTGTCTGGGCAGGTTTGACAACCAGCTCAACTCCGGTAAGGATGGAGAGCTGTTGCTCACCCTGA
CCGACGTGCTTATTAAGATG

Downstream 100 bases:

>100_bases
ATATGATTTAAACATCGCTCAGACTGTTAGCCCTGTTTTGACAGGTTAATCAGGCTAGAAAAAAGGGAAAGCGCACTAAA
AGTGCGGTAATCCCTTCGCT

Product: multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MNFKNVFRWHLPFLLLLGLFSLRAAATDTLLILGDSLSAGYRLPIAQAWPSLLDKKWQATPSLPKVVNASISGDTAAQGL
ARLPALLKQHQPRWVLIELGANDALRGFPTQDIQRDLSEIINQITAAKAQPLLMQIRIPPNYGRRYTDAFTAIYPQLAQQ
FDIPLLPFFMEQVAVKPEWMQDDGLHPNGDAQPFIADWMAQQLKPLVVDPK

Sequences:

>Translated_211_residues
MNFKNVFRWHLPFLLLLGLFSLRAAATDTLLILGDSLSAGYRLPIAQAWPSLLDKKWQATPSLPKVVNASISGDTAAQGL
ARLPALLKQHQPRWVLIELGANDALRGFPTQDIQRDLSEIINQITAAKAQPLLMQIRIPPNYGRRYTDAFTAIYPQLAQQ
FDIPLLPFFMEQVAVKPEWMQDDGLHPNGDAQPFIADWMAQQLKPLVVDPK
>Mature_211_residues
MNFKNVFRWHLPFLLLLGLFSLRAAATDTLLILGDSLSAGYRLPIAQAWPSLLDKKWQATPSLPKVVNASISGDTAAQGL
ARLPALLKQHQPRWVLIELGANDALRGFPTQDIQRDLSEIINQITAAKAQPLLMQIRIPPNYGRRYTDAFTAIYPQLAQQ
FDIPLLPFFMEQVAVKPEWMQDDGLHPNGDAQPFIADWMAQQLKPLVVDPK

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=207, Percent_Identity=71.0144927536232, Blast_Score=308, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 23675; Mature: 23675

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS01098 LIPASE_GDSL_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKNVFRWHLPFLLLLGLFSLRAAATDTLLILGDSLSAGYRLPIAQAWPSLLDKKWQAT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCC
PSLPKVVNASISGDTAAQGLARLPALLKQHQPRWVLIELGANDALRGFPTQDIQRDLSEI
CCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHH
INQITAAKAQPLLMQIRIPPNYGRRYTDAFTAIYPQLAQQFDIPLLPFFMEQVAVKPEWM
HHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
QDDGLHPNGDAQPFIADWMAQQLKPLVVDPK
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNFKNVFRWHLPFLLLLGLFSLRAAATDTLLILGDSLSAGYRLPIAQAWPSLLDKKWQAT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCC
PSLPKVVNASISGDTAAQGLARLPALLKQHQPRWVLIELGANDALRGFPTQDIQRDLSEI
CCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHH
INQITAAKAQPLLMQIRIPPNYGRRYTDAFTAIYPQLAQQFDIPLLPFFMEQVAVKPEWM
HHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
QDDGLHPNGDAQPFIADWMAQQLKPLVVDPK
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]