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The map label for this gene is dam [H]

Identifier: 153949277

GI number: 153949277

Start: 4445272

End: 4446087

Strand: Reverse

Name: dam [H]

Synonym: YpsIP31758_3963

Alternate gene names: 153949277

Gene position: 4446087-4445272 (Counterclockwise)

Preceding gene: 153949832

Following gene: 153950747

Centisome position: 94.13

GC content: 45.96

Gene sequence:

>816_bases
ATGAAGAAAAACCGCGCTTTTTTAAAATGGGCTGGTGGGAAGTATCCGCTGGTTGATGACATACGACGCCATCTTCCAGC
GGGAGATTGTTTGATAGAGCCATTCGTTGGTGCGGGTTCCGTATTTCTCAACACCGAGTTCGAATCCTACATACTGGCTG
ATATCAACAACGATCTCATCAACTTATACAATATCGTTAAGTTACGTACAGATGATTTTGTGCGTGATGCTCGAGTTCTC
TTTACTGGCGACTTCAATCATTCCGAGCTGTTTTACCAACTGCGGCAAGAATTTAATGCCAGTACGGATGCTTATCGCCG
TGCATTGCTGTTCCTCTATCTCAATCGCCACTGTTATAACGGCCTGTGTCGTTATAATTTGAGTGGTGAATTCAATGTGC
CTTTTGGTCGCTACAAAAAACCCTACTTCCCAGAAGCGGAGTTATATTGGTTTGCTGAAAAATCGCAAAATGCGGTTTTT
GTTTGTGAGCACTATCAGGAAACTTTGTTAAAAGCCGTGCAGGGAGCGGTAGTTTACTGCGATCCTCCTTATGCGCCGCT
ATCAGCGACGGCAAACTTTACGGCCTATCACACCAATAACTTTGGGATTGCAGACCAGCAAAATCTGGCGCGTCTGGCTT
ATCAGTTGTCTACTGAGAGTAAAGTTCCGGTACTGATTTCTAACCATGACACCGAACTGACGCGTAATTGGTATCATCAG
GCGGCGTCGCTACATGTCGTCACTGCGCGCCGTACGATCAGCCGTAATATCCTTGGTCGCAGTAAGGTAAACGAACTTTT
GGCGCTCTATAGCTGA

Upstream 100 bases:

>100_bases
AAGACCTTAAAAAATAAACCATTCTGATTTGTGCGCTGATGTGCTGTTTGAAACAGAGTACAATCTGCGGCTCTGAATTA
TTAAGTAGCTAACTGACGGC

Downstream 100 bases:

>100_bases
GTGAGATTGCTGTCCGGTAATCGCTCAGACGGGGAGTTTATGAAGTGATGGCGATAGTTTATCAAATGGTCGTGGCGGTT
GTTTATTAGTGGTTGTTTAT

Product: DNA adenine methylase

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase; M.SmaII [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKKNRAFLKWAGGKYPLVDDIRRHLPAGDCLIEPFVGAGSVFLNTEFESYILADINNDLINLYNIVKLRTDDFVRDARVL
FTGDFNHSELFYQLRQEFNASTDAYRRALLFLYLNRHCYNGLCRYNLSGEFNVPFGRYKKPYFPEAELYWFAEKSQNAVF
VCEHYQETLLKAVQGAVVYCDPPYAPLSATANFTAYHTNNFGIADQQNLARLAYQLSTESKVPVLISNHDTELTRNWYHQ
AASLHVVTARRTISRNILGRSKVNELLALYS

Sequences:

>Translated_271_residues
MKKNRAFLKWAGGKYPLVDDIRRHLPAGDCLIEPFVGAGSVFLNTEFESYILADINNDLINLYNIVKLRTDDFVRDARVL
FTGDFNHSELFYQLRQEFNASTDAYRRALLFLYLNRHCYNGLCRYNLSGEFNVPFGRYKKPYFPEAELYWFAEKSQNAVF
VCEHYQETLLKAVQGAVVYCDPPYAPLSATANFTAYHTNNFGIADQQNLARLAYQLSTESKVPVLISNHDTELTRNWYHQ
AASLHVVTARRTISRNILGRSKVNELLALYS
>Mature_271_residues
MKKNRAFLKWAGGKYPLVDDIRRHLPAGDCLIEPFVGAGSVFLNTEFESYILADINNDLINLYNIVKLRTDDFVRDARVL
FTGDFNHSELFYQLRQEFNASTDAYRRALLFLYLNRHCYNGLCRYNLSGEFNVPFGRYKKPYFPEAELYWFAEKSQNAVF
VCEHYQETLLKAVQGAVVYCDPPYAPLSATANFTAYHTNNFGIADQQNLARLAYQLSTESKVPVLISNHDTELTRNWYHQ
AASLHVVTARRTISRNILGRSKVNELLALYS

Specific function: Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=271, Percent_Identity=70.479704797048, Blast_Score=397, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31239; Mature: 31239

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNRAFLKWAGGKYPLVDDIRRHLPAGDCLIEPFVGAGSVFLNTEFESYILADINNDLI
CCCCCEEEEECCCCCCCHHHHHHHCCCCCEEECCCCCCCEEEEECCCCEEEEEECCCCHH
NLYNIVKLRTDDFVRDARVLFTGDFNHSELFYQLRQEFNASTDAYRRALLFLYLNRHCYN
HHHHHEEECCCHHHCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
GLCRYNLSGEFNVPFGRYKKPYFPEAELYWFAEKSQNAVFVCEHYQETLLKAVQGAVVYC
CEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHCCCEEEE
DPPYAPLSATANFTAYHTNNFGIADQQNLARLAYQLSTESKVPVLISNHDTELTRNWYHQ
CCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHH
AASLHVVTARRTISRNILGRSKVNELLALYS
HCEEEEEEHHHHHHHHHHCHHHHHHHHHHCC
>Mature Secondary Structure
MKKNRAFLKWAGGKYPLVDDIRRHLPAGDCLIEPFVGAGSVFLNTEFESYILADINNDLI
CCCCCEEEEECCCCCCCHHHHHHHCCCCCEEECCCCCCCEEEEECCCCEEEEEECCCCHH
NLYNIVKLRTDDFVRDARVLFTGDFNHSELFYQLRQEFNASTDAYRRALLFLYLNRHCYN
HHHHHEEECCCHHHCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
GLCRYNLSGEFNVPFGRYKKPYFPEAELYWFAEKSQNAVFVCEHYQETLLKAVQGAVVYC
CEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHCCCEEEE
DPPYAPLSATANFTAYHTNNFGIADQQNLARLAYQLSTESKVPVLISNHDTELTRNWYHQ
CCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHH
AASLHVVTARRTISRNILGRSKVNELLALYS
HCEEEEEEHHHHHHHHHHCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10383952 [H]