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The map label for this gene is dnaB [H]

Identifier: 153949274

GI number: 153949274

Start: 2551271

End: 2552644

Strand: Reverse

Name: dnaB [H]

Synonym: YpsIP31758_2244

Alternate gene names: 153949274

Gene position: 2552644-2551271 (Counterclockwise)

Preceding gene: 153949852

Following gene: 153948966

Centisome position: 54.04

GC content: 48.18

Gene sequence:

>1374_bases
ATGAAACCTCAGGAACTGGAAGCGGCAATTCTCTCCGGACTGTTGTATGGCGGCTCGACACCCGATGCATTGGAGGTTAT
TGCCACGTTGCCGGAAGAGGCTTTCAGCATACGGTTCTACCGTTCGGCATATGCAGAAATAAAAAAACAGGCACTGACAA
CGGGAATGATTGATGTCCTGTTTGTTAGTGAGGCTCTAGGGGGGACCAGTCTGGCTGCGCTATCTGACATCGTCCGTATG
CCGGGGAATGTGGCGAACCTGAAGGGTTATGCGGCACATGCTCGTAAGACCTGGTACAGCCGGTCTATGACGGCATTATT
TCAGACGGCTGCTGATGGGATTCGTGGCGCTAATAATCAGGAGCAACGTGATCAGGTGATCCAAAGTGCGGTAATGCAGT
TGATCGATATGACAGCAGACAGCGGCGGTATCATCCCGGTACATCTCAACGATTTACTGCCCGGCTACATCGACATATTG
GGCAAACGCATGAATGGTGAGATTGATTCAATCAACCTACAGAGTGGGATTGCTGAACTGGATGCAATAACGGGAGGGTT
TAATCCTCAGGATCTGGTTGTTATTGCAGGTCGCCCAGGAATGGGTAAAACGGAGTTTGCACTGAAGATTGTTGAGGGAG
CGACACAAAATGGTGGTGGCGCTCTGGTATTTAGCATGGAAATGGCTTCCTTGCAGATTGTCGAACGCTCTATTGCCGGG
GCCGGAAACTTGCCCGTATCAAAATTACGTGCGCCCAAGACACTATGCGATGAGGATTGGGAGCGCATTAATACTGCGCT
ACAGGCATTAAATGGGCGAGATATTTGGATTGTAGATGCCAGTGAGTTGACCATAGATCAAATTTGGGCCATTGCAGAAA
CACACAAACGCCGGTATCCAAAACTACGGATGGTGATGGTTGACTATCTTGGTTTGATCACCAAGCCAAAAGCGGAGCGT
AATGATCTGTCTCTTGGAATAATTTCACGCAGGTTAAAAACGCTGGCTGGTCGAATAAATACCCCCATTTTTGCTCTTAG
CCAGTTATCGCGAAAAGTGGATGAAAGGCCACTGGGTAATCGGAGACCAATCAATTCCGACTTGAGAGATTCAGGTTCAA
TTGAACAGGATGCTGACAGCATCATCATGTTGTATCGGGAGGCCATTTATAACCCCGATAGCCCGGCTGCAAGATTTGCT
GAAGCTATCGTAACTAAAAACAGATTTGGTGAATACGGTACCGTTTATCAGGAGTTCCAAAACGGTCACTTTCTAGCCGT
TGACCAGTTGGTGGCCAGAGAAGCCAGCCGCATGTCAAAAGAGGCAATGAAGCTCCCTGTCAGAGAAAAACGGTATTCGA
CAGCAAATTTTTAA

Upstream 100 bases:

>100_bases
CAGAGTTTTATTTCGGGGGGGCGAGTGGGGAAGGTTGGCGGGCAAACTTTGATTATCTGATGAAGCCTGAGGTTCTTAGA
AAAACCCGAGATGGTGCGTT

Downstream 100 bases:

>100_bases
TCGCACCTGACCCGCGTTGAAACGCGAAGAGGTAAGACCATGCGGACCATCAATGAAAAATCTCCGGTTGTTGAATTCAG
GGTGTAACCATGGACGGGCA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MKPQELEAAILSGLLYGGSTPDALEVIATLPEEAFSIRFYRSAYAEIKKQALTTGMIDVLFVSEALGGTSLAALSDIVRM
PGNVANLKGYAAHARKTWYSRSMTALFQTAADGIRGANNQEQRDQVIQSAVMQLIDMTADSGGIIPVHLNDLLPGYIDIL
GKRMNGEIDSINLQSGIAELDAITGGFNPQDLVVIAGRPGMGKTEFALKIVEGATQNGGGALVFSMEMASLQIVERSIAG
AGNLPVSKLRAPKTLCDEDWERINTALQALNGRDIWIVDASELTIDQIWAIAETHKRRYPKLRMVMVDYLGLITKPKAER
NDLSLGIISRRLKTLAGRINTPIFALSQLSRKVDERPLGNRRPINSDLRDSGSIEQDADSIIMLYREAIYNPDSPAARFA
EAIVTKNRFGEYGTVYQEFQNGHFLAVDQLVAREASRMSKEAMKLPVREKRYSTANF

Sequences:

>Translated_457_residues
MKPQELEAAILSGLLYGGSTPDALEVIATLPEEAFSIRFYRSAYAEIKKQALTTGMIDVLFVSEALGGTSLAALSDIVRM
PGNVANLKGYAAHARKTWYSRSMTALFQTAADGIRGANNQEQRDQVIQSAVMQLIDMTADSGGIIPVHLNDLLPGYIDIL
GKRMNGEIDSINLQSGIAELDAITGGFNPQDLVVIAGRPGMGKTEFALKIVEGATQNGGGALVFSMEMASLQIVERSIAG
AGNLPVSKLRAPKTLCDEDWERINTALQALNGRDIWIVDASELTIDQIWAIAETHKRRYPKLRMVMVDYLGLITKPKAER
NDLSLGIISRRLKTLAGRINTPIFALSQLSRKVDERPLGNRRPINSDLRDSGSIEQDADSIIMLYREAIYNPDSPAARFA
EAIVTKNRFGEYGTVYQEFQNGHFLAVDQLVAREASRMSKEAMKLPVREKRYSTANF
>Mature_457_residues
MKPQELEAAILSGLLYGGSTPDALEVIATLPEEAFSIRFYRSAYAEIKKQALTTGMIDVLFVSEALGGTSLAALSDIVRM
PGNVANLKGYAAHARKTWYSRSMTALFQTAADGIRGANNQEQRDQVIQSAVMQLIDMTADSGGIIPVHLNDLLPGYIDIL
GKRMNGEIDSINLQSGIAELDAITGGFNPQDLVVIAGRPGMGKTEFALKIVEGATQNGGGALVFSMEMASLQIVERSIAG
AGNLPVSKLRAPKTLCDEDWERINTALQALNGRDIWIVDASELTIDQIWAIAETHKRRYPKLRMVMVDYLGLITKPKAER
NDLSLGIISRRLKTLAGRINTPIFALSQLSRKVDERPLGNRRPINSDLRDSGSIEQDADSIIMLYREAIYNPDSPAARFA
EAIVTKNRFGEYGTVYQEFQNGHFLAVDQLVAREASRMSKEAMKLPVREKRYSTANF

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=441, Percent_Identity=32.6530612244898, Blast_Score=196, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50168; Mature: 50168

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPQELEAAILSGLLYGGSTPDALEVIATLPEEAFSIRFYRSAYAEIKKQALTTGMIDVL
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVSEALGGTSLAALSDIVRMPGNVANLKGYAAHARKTWYSRSMTALFQTAADGIRGANNQ
HHHHHCCCHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EQRDQVIQSAVMQLIDMTADSGGIIPVHLNDLLPGYIDILGKRMNGEIDSINLQSGIAEL
HHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCHHHHHHHHHHCCCCCEEEHHHHHHHH
DAITGGFNPQDLVVIAGRPGMGKTEFALKIVEGATQNGGGALVFSMEMASLQIVERSIAG
HHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHCC
AGNLPVSKLRAPKTLCDEDWERINTALQALNGRDIWIVDASELTIDQIWAIAETHKRRYP
CCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
KLRMVMVDYLGLITKPKAERNDLSLGIISRRLKTLAGRINTPIFALSQLSRKVDERPLGN
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCC
RRPINSDLRDSGSIEQDADSIIMLYREAIYNPDSPAARFAEAIVTKNRFGEYGTVYQEFQ
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHC
NGHFLAVDQLVAREASRMSKEAMKLPVREKRYSTANF
CCCEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCC
>Mature Secondary Structure
MKPQELEAAILSGLLYGGSTPDALEVIATLPEEAFSIRFYRSAYAEIKKQALTTGMIDVL
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVSEALGGTSLAALSDIVRMPGNVANLKGYAAHARKTWYSRSMTALFQTAADGIRGANNQ
HHHHHCCCHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EQRDQVIQSAVMQLIDMTADSGGIIPVHLNDLLPGYIDILGKRMNGEIDSINLQSGIAEL
HHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCHHHHHHHHHHCCCCCEEEHHHHHHHH
DAITGGFNPQDLVVIAGRPGMGKTEFALKIVEGATQNGGGALVFSMEMASLQIVERSIAG
HHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHCC
AGNLPVSKLRAPKTLCDEDWERINTALQALNGRDIWIVDASELTIDQIWAIAETHKRRYP
CCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
KLRMVMVDYLGLITKPKAERNDLSLGIISRRLKTLAGRINTPIFALSQLSRKVDERPLGN
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCC
RRPINSDLRDSGSIEQDADSIIMLYREAIYNPDSPAARFAEAIVTKNRFGEYGTVYQEFQ
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHC
NGHFLAVDQLVAREASRMSKEAMKLPVREKRYSTANF
CCCEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]