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The map label for this gene is mleN [H]

Identifier: 153948910

GI number: 153948910

Start: 638521

End: 639972

Strand: Direct

Name: mleN [H]

Synonym: YpsIP31758_0539

Alternate gene names: 153948910

Gene position: 638521-639972 (Clockwise)

Preceding gene: 153948598

Following gene: 153948108

Centisome position: 13.52

GC content: 43.53

Gene sequence:

>1452_bases
ATGGATAATGAAGGACTCATTTTTCGCGGCGGAAAAAATATGGCATTACTGCCGATGCTGCTTTTTATTGTGTCGTGTTC
ACTATTCTTTTCAGTTTTCCATGTCTTTGACATGACCGCATTGGCGATGCTCGCATTTCTTGCAATTATGATAGGGGCAA
TCTTTTCACGTAATGTTAGTCATTATTGGAAAGCCGTTATTTCTGAAGGTATTGGCAGTGAAATGGCTGTCACTATTGCT
ACAATCCTATTAATTATCGGCATGTATGCGAAAATGATCGCTCAGTCCGGTGTTGCAGATAGTTTTGCCCTGTTGGCGAA
TCATCTGGGATTGCATGGCGGCGCTTTTACATTATTTACTTTTATTTCTGTTTGTATTGTCGCCACGGCAACGGGGACAT
CGGTAGGAACGCTATTTACTGCTTTCCCCGTATTTTATCCTTCCGGCATTTTACTGGGGGCAGATCCTGTTGTATTAGCC
AGTGCTATTCTGTGTGGTGCTATTTTTGGCGACAATATTGCCCCAATATCGGATACCACAGTTGCATCCTCCTCAACCCA
AACTTACAAGCATAAGCCAGGTAGCGCGGAAATTGCAGGTGTAGTCGCCGCGCGTTTTCGATTTGCGATAGTCAGTGCTA
TCATCGCGGGAGCTCTATTTTATTTCTTAGGCTCCAATAATATACCTCTCGCAGAAAGCAGCCTTGTCCGCGATAGTCAG
GCATCTTACCGAAGTTTATGGATGCTGGTTCCTATGGCGGTACTGTTAATTGTTGCGATGAAAACTCGTAATATCTTTAA
AGCGATCCCTTCAGGGATACTCACAGGGATAATTATCGGTTTACTGTCCGGTATTTTTAACTTCACGGATATATTTGCAC
TGGATAACGGGAATGTCACCGGTTTTCTGTACAATGGATTCCATGAGATGGTCAGTACTATTCTCTTTGTCCTTTCTTTG
TTTGGCATCATTGGTATTCTGCGTGCCAGTAAAACCATGGATCGTATTTCTGCGGCTATCTGTAGTAGTCGACTGGCTCA
ATCAGCTCAAGGCGCAGAATGGTGTATTGCTATTGGCTCCATTATCGCAACCGCATTAGTTGGCGGTGTAACCAGCGCAT
CTATTTTGGCTTTTGGTCCTGTAGCTAATGAAATCGGGTTACGTAAGTCATTACACCCCTATCGTCGCGCGGTCCTGGTT
GACTGTTTTGCCATGACTCTGGCAGCGATCATTCCCTTCCTTAGTGCTTTTATTTTTATAGTCAGTGCGATCATCAGTTC
TTTACAAAAAGAATATCCATTTATTCAACCAGTTAACCCCATTGATCTTTCACTGGCAGCTTTCTATCCTCTGACACTTT
TCGTCGTGATGTCATTCTCGATCATCGTTGGTTGGGGGCGTAGCTTTGAAAGTAAAGATGGTTCATTAGTCAAGAGTCTC
CCCCTAAATTAA

Upstream 100 bases:

>100_bases
CTGGTACATGGTTGTTATAAAGATGATGAGAAACTCTATGCAGTTTTGACTCGAATCCAACAGGCATTAATGCAGTTAGC
GAAGGAAAAGGGGATCTGCC

Downstream 100 bases:

>100_bases
TGGATGGTTAACATGATGGAATGTATCACTGTTAGTGATGTAATCAATGTTTCTGTGGAAGAAGTCTGGAAGAAAATAAG
CGCTTTTGATGAATTTTCAG

Product: Na+/H+ antiporter family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MDNEGLIFRGGKNMALLPMLLFIVSCSLFFSVFHVFDMTALAMLAFLAIMIGAIFSRNVSHYWKAVISEGIGSEMAVTIA
TILLIIGMYAKMIAQSGVADSFALLANHLGLHGGAFTLFTFISVCIVATATGTSVGTLFTAFPVFYPSGILLGADPVVLA
SAILCGAIFGDNIAPISDTTVASSSTQTYKHKPGSAEIAGVVAARFRFAIVSAIIAGALFYFLGSNNIPLAESSLVRDSQ
ASYRSLWMLVPMAVLLIVAMKTRNIFKAIPSGILTGIIIGLLSGIFNFTDIFALDNGNVTGFLYNGFHEMVSTILFVLSL
FGIIGILRASKTMDRISAAICSSRLAQSAQGAEWCIAIGSIIATALVGGVTSASILAFGPVANEIGLRKSLHPYRRAVLV
DCFAMTLAAIIPFLSAFIFIVSAIISSLQKEYPFIQPVNPIDLSLAAFYPLTLFVVMSFSIIVGWGRSFESKDGSLVKSL
PLN

Sequences:

>Translated_483_residues
MDNEGLIFRGGKNMALLPMLLFIVSCSLFFSVFHVFDMTALAMLAFLAIMIGAIFSRNVSHYWKAVISEGIGSEMAVTIA
TILLIIGMYAKMIAQSGVADSFALLANHLGLHGGAFTLFTFISVCIVATATGTSVGTLFTAFPVFYPSGILLGADPVVLA
SAILCGAIFGDNIAPISDTTVASSSTQTYKHKPGSAEIAGVVAARFRFAIVSAIIAGALFYFLGSNNIPLAESSLVRDSQ
ASYRSLWMLVPMAVLLIVAMKTRNIFKAIPSGILTGIIIGLLSGIFNFTDIFALDNGNVTGFLYNGFHEMVSTILFVLSL
FGIIGILRASKTMDRISAAICSSRLAQSAQGAEWCIAIGSIIATALVGGVTSASILAFGPVANEIGLRKSLHPYRRAVLV
DCFAMTLAAIIPFLSAFIFIVSAIISSLQKEYPFIQPVNPIDLSLAAFYPLTLFVVMSFSIIVGWGRSFESKDGSLVKSL
PLN
>Mature_483_residues
MDNEGLIFRGGKNMALLPMLLFIVSCSLFFSVFHVFDMTALAMLAFLAIMIGAIFSRNVSHYWKAVISEGIGSEMAVTIA
TILLIIGMYAKMIAQSGVADSFALLANHLGLHGGAFTLFTFISVCIVATATGTSVGTLFTAFPVFYPSGILLGADPVVLA
SAILCGAIFGDNIAPISDTTVASSSTQTYKHKPGSAEIAGVVAARFRFAIVSAIIAGALFYFLGSNNIPLAESSLVRDSQ
ASYRSLWMLVPMAVLLIVAMKTRNIFKAIPSGILTGIIIGLLSGIFNFTDIFALDNGNVTGFLYNGFHEMVSTILFVLSL
FGIIGILRASKTMDRISAAICSSRLAQSAQGAEWCIAIGSIIATALVGGVTSASILAFGPVANEIGLRKSLHPYRRAVLV
DCFAMTLAAIIPFLSAFIFIVSAIISSLQKEYPFIQPVNPIDLSLAAFYPLTLFVVMSFSIIVGWGRSFESKDGSLVKSL
PLN

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 51282; Mature: 51282

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNEGLIFRGGKNMALLPMLLFIVSCSLFFSVFHVFDMTALAMLAFLAIMIGAIFSRNVS
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
HYWKAVISEGIGSEMAVTIATILLIIGMYAKMIAQSGVADSFALLANHLGLHGGAFTLFT
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHH
FISVCIVATATGTSVGTLFTAFPVFYPSGILLGADPVVLASAILCGAIFGDNIAPISDTT
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCC
VASSSTQTYKHKPGSAEIAGVVAARFRFAIVSAIIAGALFYFLGSNNIPLAESSLVRDSQ
CCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
ASYRSLWMLVPMAVLLIVAMKTRNIFKAIPSGILTGIIIGLLSGIFNFTDIFALDNGNVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEE
GFLYNGFHEMVSTILFVLSLFGIIGILRASKTMDRISAAICSSRLAQSAQGAEWCIAIGS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IIATALVGGVTSASILAFGPVANEIGLRKSLHPYRRAVLVDCFAMTLAAIIPFLSAFIFI
HHHHHHHHCCHHHHHHEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSAIISSLQKEYPFIQPVNPIDLSLAAFYPLTLFVVMSFSIIVGWGRSFESKDGSLVKSL
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCC
PLN
CCC
>Mature Secondary Structure
MDNEGLIFRGGKNMALLPMLLFIVSCSLFFSVFHVFDMTALAMLAFLAIMIGAIFSRNVS
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
HYWKAVISEGIGSEMAVTIATILLIIGMYAKMIAQSGVADSFALLANHLGLHGGAFTLFT
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHH
FISVCIVATATGTSVGTLFTAFPVFYPSGILLGADPVVLASAILCGAIFGDNIAPISDTT
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCC
VASSSTQTYKHKPGSAEIAGVVAARFRFAIVSAIIAGALFYFLGSNNIPLAESSLVRDSQ
CCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
ASYRSLWMLVPMAVLLIVAMKTRNIFKAIPSGILTGIIIGLLSGIFNFTDIFALDNGNVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEE
GFLYNGFHEMVSTILFVLSLFGIIGILRASKTMDRISAAICSSRLAQSAQGAEWCIAIGS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IIATALVGGVTSASILAFGPVANEIGLRKSLHPYRRAVLVDCFAMTLAAIIPFLSAFIFI
HHHHHHHHCCHHHHHHEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSAIISSLQKEYPFIQPVNPIDLSLAAFYPLTLFVVMSFSIIVGWGRSFESKDGSLVKSL
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCC
PLN
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]