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The map label for this gene is rhtB [H]

Identifier: 153948903

GI number: 153948903

Start: 247991

End: 248611

Strand: Reverse

Name: rhtB [H]

Synonym: YpsIP31758_0220

Alternate gene names: 153948903

Gene position: 248611-247991 (Counterclockwise)

Preceding gene: 153950696

Following gene: 153950232

Centisome position: 5.26

GC content: 48.15

Gene sequence:

>621_bases
ATGACCTTAGACTGGTGGTTAACCTATCTGCTGACAACATTGATCCTCAGTCTTTCTCCCGGTTCGGGGGCCATTAACAC
GATGAGCACTGCCATCAGCCATGGTACGCGTGGGGTCTTTGCTTCCATTTGCGGGTTACAACTGGGCCTGGCGGTGCATA
TCGTCTTGGTTGGCGTCGGTCTTGGTGCCCTGGTCTCTCAATCCTTACTGGCATTTGAAATATTAAAGTGGCTCGGTGCG
GCTTACTTAATTTGGCTTGGTATTCAACAATGGCGTGCAGCCGGGTCGCTTGACCTGCATGCACTGGCAAACAGTATGCC
CAGGCGTAAGCTGTTTAAACGTGCGGTGTTTGTTAACCTGACCAATCCAAAAAGCATTGTTTTTCTGGCCGCGTTATTTC
CACAATTTGTACTGCCACAGCAACCGCAGGTGGCACAGTATTTGATTTTGGGCAGCACCAGTGTGATTGTCGATATTATC
GTGATGATTGGTTATGCCACTTTGGCTACACGTATTGCCCGTTGGATTAAATCGCCACAGCAAATGAAACTCTTGAACCG
AATATTTGGCGGCCTATTTATGTTGATTGGTGCATTATTAGCCACAGCGCGCAAAGTATAA

Upstream 100 bases:

>100_bases
CATGCTTTAGCCGACGTTACTCAGAGTTTTGTGAGCCATAGCACAACTATTGGCAATGGGGTATTGTGGCATAAATTTAC
CCATTCAGGGAGCTTTGCCG

Downstream 100 bases:

>100_bases
AGAGCCTGTCAGGCTAGCCAACCGGGTTAAATACCCCGTTGGCTAACCCGTTAGCGGGACAATATCAGTTGGATACCAAA
ACCGGCAAATAACACACCTG

Product: homoserine/homoserine lactone efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTLDWWLTYLLTTLILSLSPGSGAINTMSTAISHGTRGVFASICGLQLGLAVHIVLVGVGLGALVSQSLLAFEILKWLGA
AYLIWLGIQQWRAAGSLDLHALANSMPRRKLFKRAVFVNLTNPKSIVFLAALFPQFVLPQQPQVAQYLILGSTSVIVDII
VMIGYATLATRIARWIKSPQQMKLLNRIFGGLFMLIGALLATARKV

Sequences:

>Translated_206_residues
MTLDWWLTYLLTTLILSLSPGSGAINTMSTAISHGTRGVFASICGLQLGLAVHIVLVGVGLGALVSQSLLAFEILKWLGA
AYLIWLGIQQWRAAGSLDLHALANSMPRRKLFKRAVFVNLTNPKSIVFLAALFPQFVLPQQPQVAQYLILGSTSVIVDII
VMIGYATLATRIARWIKSPQQMKLLNRIFGGLFMLIGALLATARKV
>Mature_205_residues
TLDWWLTYLLTTLILSLSPGSGAINTMSTAISHGTRGVFASICGLQLGLAVHIVLVGVGLGALVSQSLLAFEILKWLGAA
YLIWLGIQQWRAAGSLDLHALANSMPRRKLFKRAVFVNLTNPKSIVFLAALFPQFVLPQQPQVAQYLILGSTSVIVDIIV
MIGYATLATRIARWIKSPQQMKLLNRIFGGLFMLIGALLATARKV

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=205, Percent_Identity=74.6341463414634, Blast_Score=322, Evalue=9e-90,
Organism=Escherichia coli, GI1788099, Length=203, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22415; Mature: 22284

Theoretical pI: Translated: 11.39; Mature: 11.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDWWLTYLLTTLILSLSPGSGAINTMSTAISHGTRGVFASICGLQLGLAVHIVLVGVG
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LGALVSQSLLAFEILKWLGAAYLIWLGIQQWRAAGSLDLHALANSMPRRKLFKRAVFVNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHEEC
TNPKSIVFLAALFPQFVLPQQPQVAQYLILGSTSVIVDIIVMIGYATLATRIARWIKSPQ
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
QMKLLNRIFGGLFMLIGALLATARKV
HHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLDWWLTYLLTTLILSLSPGSGAINTMSTAISHGTRGVFASICGLQLGLAVHIVLVGVG
CHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LGALVSQSLLAFEILKWLGAAYLIWLGIQQWRAAGSLDLHALANSMPRRKLFKRAVFVNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHEEC
TNPKSIVFLAALFPQFVLPQQPQVAQYLILGSTSVIVDIIVMIGYATLATRIARWIKSPQ
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
QMKLLNRIFGGLFMLIGALLATARKV
HHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]