Click here to switch to the map view.

The map label for this gene is thiG

Identifier: 153948781

GI number: 153948781

Start: 4342725

End: 4343540

Strand: Direct

Name: thiG

Synonym: YpsIP31758_3856

Alternate gene names: 153948781

Gene position: 4342725-4343540 (Clockwise)

Preceding gene: 153948298

Following gene: 153948169

Centisome position: 91.94

GC content: 53.8

Gene sequence:

>816_bases
ATGCTGAAAATTGCCGATACCACTTTTACGTCTCGTTTATTTACGGGCACAGGAAAGTTCTCCTCTCCCGAACTGATGCT
GGAAGCACTCCGCGCCTCGGGTTCACAACTTATTACCATGGCCATGAAGCGCGTTGACTTACAGTCAGGAAACGACGCGA
TCCTCGCACCCTTGCGGCAGTTGGGCGTGCGCTTACTGCCTAATACATCCGGTGCCAAAACCGCTGAGGAAGCTATTTTT
GCCGCACGTCTGGCCCGCGAAGCCTTGAATACCCATTGGGTAAAACTGGAAATTCATCCAGATGTGAGATATTTGCTGCC
TGACCCGATAGAAACGCTCAAGGCCGCCGAAGTGCTGGTGAAAGAGGGGTTTGTTGTTTTACCTTACTGTGGTGCTGACC
CCGTCTTGTGTAAACGACTGGAAGAAGTGGGTTGTGCTGCGGTGATGCCATTAGGCTCACCGATAGGATCTAATTTGGGG
TTACGTACTCGCGACTTCCTGCAAATTATTATCGAACAATCGAAAGTCCCGGTCGTCGTTGATGCGGGCATTGGTGCACC
GAGCCATGCCCTTGAAGCGCTGGAACTGGGTGCCGATGCCGTACTGGTGAATACCGCAATTGCCGTAGCTCACTCCCCGG
TGCAGATGGCCCATGCTTTCCGGCTGGCGGTTGAATCTGGCGAACGGGCACGCTTGGCTGGGCTTGGCGCTTCACCGTTC
AATCCTTCACAACCTGATACATTACAACTACGGGCCACCGCCACCAGCCCGCTAACGGGTTTCCTCAGCCAACTTGAGGA
GCAAGACCATGTCTGA

Upstream 100 bases:

>100_bases
GCTATTAATCAGGTTATTATTCCGCGCGCCGATTGGCAAAATCATCGGTTGCAAGCGGGTGATCATATCTTGCTGTTCCA
AGCCATTGCCGGAGGCTGAC

Downstream 100 bases:

>100_bases
AGACTTCAACCAGCGCTGGCAGCAGTTGGACTGGGACGATATCTCCTTAACCATCAACAGTAAAAAACCCGCAGACGTTG
AACGGGCGCTGAATGCCATC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MLKIADTTFTSRLFTGTGKFSSPELMLEALRASGSQLITMAMKRVDLQSGNDAILAPLRQLGVRLLPNTSGAKTAEEAIF
AARLAREALNTHWVKLEIHPDVRYLLPDPIETLKAAEVLVKEGFVVLPYCGADPVLCKRLEEVGCAAVMPLGSPIGSNLG
LRTRDFLQIIIEQSKVPVVVDAGIGAPSHALEALELGADAVLVNTAIAVAHSPVQMAHAFRLAVESGERARLAGLGASPF
NPSQPDTLQLRATATSPLTGFLSQLEEQDHV

Sequences:

>Translated_271_residues
MLKIADTTFTSRLFTGTGKFSSPELMLEALRASGSQLITMAMKRVDLQSGNDAILAPLRQLGVRLLPNTSGAKTAEEAIF
AARLAREALNTHWVKLEIHPDVRYLLPDPIETLKAAEVLVKEGFVVLPYCGADPVLCKRLEEVGCAAVMPLGSPIGSNLG
LRTRDFLQIIIEQSKVPVVVDAGIGAPSHALEALELGADAVLVNTAIAVAHSPVQMAHAFRLAVESGERARLAGLGASPF
NPSQPDTLQLRATATSPLTGFLSQLEEQDHV
>Mature_271_residues
MLKIADTTFTSRLFTGTGKFSSPELMLEALRASGSQLITMAMKRVDLQSGNDAILAPLRQLGVRLLPNTSGAKTAEEAIF
AARLAREALNTHWVKLEIHPDVRYLLPDPIETLKAAEVLVKEGFVVLPYCGADPVLCKRLEEVGCAAVMPLGSPIGSNLG
LRTRDFLQIIIEQSKVPVVVDAGIGAPSHALEALELGADAVLVNTAIAVAHSPVQMAHAFRLAVESGERARLAGLGASPF
NPSQPDTLQLRATATSPLTGFLSQLEEQDHV

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=262, Percent_Identity=74.4274809160305, Blast_Score=383, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_YERP3 (A7FNH9)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402807.1
- ProteinModelPortal:   A7FNH9
- SMR:   A7FNH9
- STRING:   A7FNH9
- GeneID:   5388204
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3856
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- BioCyc:   YPSE349747:YPSIP31758_3856-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 28888; Mature: 28888

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: ACT_SITE 95-95 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIADTTFTSRLFTGTGKFSSPELMLEALRASGSQLITMAMKRVDLQSGNDAILAPLRQ
CEEECCCHHHHHEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
LGVRLLPNTSGAKTAEEAIFAARLAREALNTHWVKLEIHPDVRYLLPDPIETLKAAEVLV
CCEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCCHHHHHHHHHHHH
KEGFVVLPYCGADPVLCKRLEEVGCAAVMPLGSPIGSNLGLRTRDFLQIIIEQSKVPVVV
HCCCEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
DAGIGAPSHALEALELGADAVLVNTAIAVAHSPVQMAHAFRLAVESGERARLAGLGASPF
ECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCC
NPSQPDTLQLRATATSPLTGFLSQLEEQDHV
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKIADTTFTSRLFTGTGKFSSPELMLEALRASGSQLITMAMKRVDLQSGNDAILAPLRQ
CEEECCCHHHHHEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
LGVRLLPNTSGAKTAEEAIFAARLAREALNTHWVKLEIHPDVRYLLPDPIETLKAAEVLV
CCEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCCHHHHHHHHHHHH
KEGFVVLPYCGADPVLCKRLEEVGCAAVMPLGSPIGSNLGLRTRDFLQIIIEQSKVPVVV
HCCCEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
DAGIGAPSHALEALELGADAVLVNTAIAVAHSPVQMAHAFRLAVESGERARLAGLGASPF
ECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCC
NPSQPDTLQLRATATSPLTGFLSQLEEQDHV
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA