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The map label for this gene is cusS [H]

Identifier: 153948780

GI number: 153948780

Start: 2394700

End: 2396085

Strand: Direct

Name: cusS [H]

Synonym: YpsIP31758_2085

Alternate gene names: 153948780

Gene position: 2394700-2396085 (Clockwise)

Preceding gene: 153947115

Following gene: 153948427

Centisome position: 50.7

GC content: 51.37

Gene sequence:

>1386_bases
ATGATTAAGCGTTCTATCTCTGGGCGGCTGGCGCTAATGTTTGCCTTGGCCTCCATACTGATAGTTTCGGCTCTCGGTAT
TGTACTGCGAAGCTCCTTGCATAATTCATTGCAAAAGCAGATGCATAACGAATTGCAATTCAGGGAGTCGCTGATGGCAC
CGTGGATCCTCTCCCGTACCACGCTGGACGGCTGGCAAACGTTGGCGACCAAATTCACCGATTTGGCCGCTGCTGAGGGC
GGCCGCGTACAATACTGGGTCCTCAGTGATGACCCCCAGCTTCAGGTAGGCGGCCCGGCACCCCGTGGTGTAACCTGGGC
TTCGTTAAGCAAAGGATTCAGCAAAGTACCCGGTGCGACAGACGAGTCATGCTCCCTATTTCTACTGATGTCGGAAATTC
CTGCCCAAGGTGAGCGCCCCGCTCTGCGCTATGTGGTCGCTATCGATTCCACGCCGTATATGGGTACTCTGAATGAATTT
ACCCGCACGCTGATCGTCATTACTGCGATTAGCGTCTTATTCGTGGCACTGCTGGGGTTCGTGATTGCCAGAATAGGTAT
GCGCCCTGTTAATAAACTCAGCGAACAGGCCCAGCACCTTGCGCCAGGAAATCACGGGCAGCGTCTGGATACCTTAACTC
TGCCGGATGAACTGCAAAAACTGGCCGTCGCATTCAACGGCGTGCTCGAACGGCAGGAAATCGCCTGGCGGCAACTTGAA
AGTTTTAATGCTGATGTCGCACATGAACTGAGAACCCCGTTGACCAATCTGATGGGGCAAACGCAATTAGGTCTATCTCG
TAAACGTGAAACGCATGAGCTCGAAGATTTACTGGAGTCCAATCTGGAAGAACTCGAGAGAATGACCTCTATTGTTAACG
ATATGCTGTTCCTCTCCCATGCTCAAGCTGGGGAGCACGCAACCCAGTTAACCGACGTGTCTCTTCGGGAAGAGACCTAT
AAGACCACGGAGTATGTAGAACCTTCATTTGCCGAAAAAAATTTGTCTATTCATGTTGAGGGAGATATCACTGTACGCAT
TGATCGGCGGCTTTTCCACCGCTCACTGGCGAATCTGCTAGAAAACAGCGCCCGCCATGCTGTTACTGGTACTACCGTGA
AAGTGACGATCTCCAGAGAAAATAATCTGGCATCCATCGCGGTAGCCAACATTGGAGCTCCCATTGCGGCGGAACATCTA
AACCGTTTGTTTGAGCGTTTTTATCGTGTTGATTCTTCCCGTGCACGCAGTGACACACACCATGGACTCGGGCTCTCCAT
CGTCAGGGCGGTGGCGATAATGCATCGTGGAGATGTGTTTGCCAGTAGCCAGAATGGGATTAATACCTTTGGTCTGACCC
TTGCAATAACTGACCCTTATATATAG

Upstream 100 bases:

>100_bases
TAGATACGCCCTACCCGGTCAAACTGCTGCATACAGTGCGAGGCATGGGGTATGTCCTTGAAACTCGCCCCGAACGCCAG
ACTAAATAGAGAGAGTGATC

Downstream 100 bases:

>100_bases
ACAGTGAGTCCGTTGAAAAAGAAATGAGTCGTTATGTGAAGTCTGGCCTGGCTGAGCGAGGGTAACGTCTATGACAGTGT
ATTTTTGCATACAGGTTTAA

Product: heavy metal sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MIKRSISGRLALMFALASILIVSALGIVLRSSLHNSLQKQMHNELQFRESLMAPWILSRTTLDGWQTLATKFTDLAAAEG
GRVQYWVLSDDPQLQVGGPAPRGVTWASLSKGFSKVPGATDESCSLFLLMSEIPAQGERPALRYVVAIDSTPYMGTLNEF
TRTLIVITAISVLFVALLGFVIARIGMRPVNKLSEQAQHLAPGNHGQRLDTLTLPDELQKLAVAFNGVLERQEIAWRQLE
SFNADVAHELRTPLTNLMGQTQLGLSRKRETHELEDLLESNLEELERMTSIVNDMLFLSHAQAGEHATQLTDVSLREETY
KTTEYVEPSFAEKNLSIHVEGDITVRIDRRLFHRSLANLLENSARHAVTGTTVKVTISRENNLASIAVANIGAPIAAEHL
NRLFERFYRVDSSRARSDTHHGLGLSIVRAVAIMHRGDVFASSQNGINTFGLTLAITDPYI

Sequences:

>Translated_461_residues
MIKRSISGRLALMFALASILIVSALGIVLRSSLHNSLQKQMHNELQFRESLMAPWILSRTTLDGWQTLATKFTDLAAAEG
GRVQYWVLSDDPQLQVGGPAPRGVTWASLSKGFSKVPGATDESCSLFLLMSEIPAQGERPALRYVVAIDSTPYMGTLNEF
TRTLIVITAISVLFVALLGFVIARIGMRPVNKLSEQAQHLAPGNHGQRLDTLTLPDELQKLAVAFNGVLERQEIAWRQLE
SFNADVAHELRTPLTNLMGQTQLGLSRKRETHELEDLLESNLEELERMTSIVNDMLFLSHAQAGEHATQLTDVSLREETY
KTTEYVEPSFAEKNLSIHVEGDITVRIDRRLFHRSLANLLENSARHAVTGTTVKVTISRENNLASIAVANIGAPIAAEHL
NRLFERFYRVDSSRARSDTHHGLGLSIVRAVAIMHRGDVFASSQNGINTFGLTLAITDPYI
>Mature_461_residues
MIKRSISGRLALMFALASILIVSALGIVLRSSLHNSLQKQMHNELQFRESLMAPWILSRTTLDGWQTLATKFTDLAAAEG
GRVQYWVLSDDPQLQVGGPAPRGVTWASLSKGFSKVPGATDESCSLFLLMSEIPAQGERPALRYVVAIDSTPYMGTLNEF
TRTLIVITAISVLFVALLGFVIARIGMRPVNKLSEQAQHLAPGNHGQRLDTLTLPDELQKLAVAFNGVLERQEIAWRQLE
SFNADVAHELRTPLTNLMGQTQLGLSRKRETHELEDLLESNLEELERMTSIVNDMLFLSHAQAGEHATQLTDVSLREETY
KTTEYVEPSFAEKNLSIHVEGDITVRIDRRLFHRSLANLLENSARHAVTGTTVKVTISRENNLASIAVANIGAPIAAEHL
NRLFERFYRVDSSRARSDTHHGLGLSIVRAVAIMHRGDVFASSQNGINTFGLTLAITDPYI

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=322, Percent_Identity=35.4037267080745, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788279, Length=316, Percent_Identity=30.379746835443, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1788393, Length=259, Percent_Identity=31.6602316602317, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786600, Length=250, Percent_Identity=28.4, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787894, Length=305, Percent_Identity=27.5409836065574, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790346, Length=262, Percent_Identity=27.8625954198473, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI145693157, Length=212, Percent_Identity=27.8301886792453, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1789403, Length=289, Percent_Identity=26.2975778546713, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790551, Length=310, Percent_Identity=27.741935483871, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1786912, Length=254, Percent_Identity=26.7716535433071, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1790861, Length=231, Percent_Identity=25.974025974026, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788713, Length=231, Percent_Identity=25.974025974026, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1789808, Length=195, Percent_Identity=28.7179487179487, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51039; Mature: 51039

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRSISGRLALMFALASILIVSALGIVLRSSLHNSLQKQMHNELQFRESLMAPWILSRT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
TLDGWQTLATKFTDLAAAEGGRVQYWVLSDDPQLQVGGPAPRGVTWASLSKGFSKVPGAT
CHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCHHHHHCCHHHCCCCC
DESCSLFLLMSEIPAQGERPALRYVVAIDSTPYMGTLNEFTRTLIVITAISVLFVALLGF
CCCCEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VIARIGMRPVNKLSEQAQHLAPGNHGQRLDTLTLPDELQKLAVAFNGVLERQEIAWRQLE
HHHHHCCCHHHHHHHHHHHCCCCCCCCEECEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
SFNADVAHELRTPLTNLMGQTQLGLSRKRETHELEDLLESNLEELERMTSIVNDMLFLSH
HCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQAGEHATQLTDVSLREETYKTTEYVEPSFAEKNLSIHVEGDITVRIDRRLFHRSLANLL
HCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEEHHHHHHHHHHHHH
ENSARHAVTGTTVKVTISRENNLASIAVANIGAPIAAEHLNRLFERFYRVDSSRARSDTH
HCCCCCEEECCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHCCCCC
HGLGLSIVRAVAIMHRGDVFASSQNGINTFGLTLAITDPYI
CCCHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEEEECCCC
>Mature Secondary Structure
MIKRSISGRLALMFALASILIVSALGIVLRSSLHNSLQKQMHNELQFRESLMAPWILSRT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
TLDGWQTLATKFTDLAAAEGGRVQYWVLSDDPQLQVGGPAPRGVTWASLSKGFSKVPGAT
CHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCHHHHHCCHHHCCCCC
DESCSLFLLMSEIPAQGERPALRYVVAIDSTPYMGTLNEFTRTLIVITAISVLFVALLGF
CCCCEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VIARIGMRPVNKLSEQAQHLAPGNHGQRLDTLTLPDELQKLAVAFNGVLERQEIAWRQLE
HHHHHCCCHHHHHHHHHHHCCCCCCCCEECEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
SFNADVAHELRTPLTNLMGQTQLGLSRKRETHELEDLLESNLEELERMTSIVNDMLFLSH
HCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQAGEHATQLTDVSLREETYKTTEYVEPSFAEKNLSIHVEGDITVRIDRRLFHRSLANLL
HCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEEHHHHHHHHHHHHH
ENSARHAVTGTTVKVTISRENNLASIAVANIGAPIAAEHLNRLFERFYRVDSSRARSDTH
HCCCCCEEECCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHCCCCC
HGLGLSIVRAVAIMHRGDVFASSQNGINTFGLTLAITDPYI
CCCHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]