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The map label for this gene is ligB

Identifier: 153948758

GI number: 153948758

Start: 50861

End: 52564

Strand: Direct

Name: ligB

Synonym: YpsIP31758_0042

Alternate gene names: 153948758

Gene position: 50861-52564 (Clockwise)

Preceding gene: 153947870

Following gene: 153947220

Centisome position: 1.08

GC content: 48.06

Gene sequence:

>1704_bases
ATGAACATACTGAATCTAAAGATAATAATGTTCCTATTAATATCTAACATCATAGTGGTGGGCGGTGCTTGGGCTACATC
AACATGCCCTGATTGGCCTGCAACCAGAATAGCGGTTGAGATTAACGCGTTGGAGCAACAGTTGAATAAGTGGTCGGCCG
CTTACCATCAGCAAGGTCATAGCCCGGTCACGGATGACATTTATGATCAATTACAGGACAAATTACGTGTGTGGCAATCC
TGTAGGGGGTTACCTGATAAGACGGAAAGCCAGCCGATCCCCGGCAAAGGGCAATTTCTTCACCCAGTTGCCCATACCGG
GTTAAAAAAACTCAAAGATGAAACCGCCCTCACAAGGTGGATGGCGGGGCGGAAAAATCTCTGGGTACAACCCAAGGTCG
ACGGTGTGGCAGTGACACTGGTTTATCACGGGGGGAAATTGGTGCAACTTCTTAGTCGAGGAAATGGAGTAAAAGGTCAA
AACTGGACAGAAAAAGCGCCATTTATTTCTGCTATTCCTCAATATATTGCAAATGCGCCTGCACTATTAACGTTGCAAGG
GGAGTTGTTTCTACTGATGGATGGCCACCAGCAAGCAAAATCGGGTGGGGTGAATGCCAGATCAACGGTGGCAGGTGCAC
TGATGCGCAAATCTCCGTCTCCGCTATTAGCTCAAGTTGGGGTCTTTATTTGGGCATGGCCTGATGGCCCAACAACCATG
AAAGAGAAGGTGGCACTATTACAGGTAATGGGTTTTCCTTTTACAGCTAAATACAGCGAGCCCGTCATGTCGCATCTTGA
TGTTGTGCAATGGCGTCAGTTCTGGTTTCAAGCACCACTGCCGTTTGTCACCGATGGTGTTGTGGTCAGGCAAGAGGAGG
AGCCAGCCGGTCGTTATTGGCAGGCAACACCGGGGCAGTGGTCTATGGCGTGGAAGTATCCGCCTTTGCAACACATTGCC
GAAGTAAAAGATATTCATTTTACCCTTGGACGAACGGGTAAAGGCACGGTGGTATTAGAGGTTTTACCGATAAAAATTGA
TGATAAATGGATTCGTCGAGTGAATATTGGTTCGGTCACCCGTTGGAAACAATGGGACATCGCTCCTGGTGATCACATTA
CATTGGCACTTGCCGGGCATGGTATCCCTCGGCTAGATAACGTTGTCTGGCGAGTACACCAACGGAATACTATTACGGCA
CCCAATTGGGATAAATTCCATCAGCTCAGTTGCTTTCAACGCTTACCACATGGCTGTGAACCTCAGTTTTTATCCCGCTT
GATATGGCTTAGTGGCCCAGGGGGGCTAGATATTGGCGGCATCGGTGGCGGCTTTTGGCAAGAGCTTATTCACCATGAGT
TAATTAATGATTTGGTCGGATGGTTATTACTAACACCAGAGCAAATTGCAAGTATCCCTGGAATTGGAAATGCGCGGGCT
GAAAAAATTTATCAACAGTTTCAACGGGCAAAGCAGCAACCCTTTTCTCGTTGGTTGCTGGCATTGGGGTTCCCGCAGGT
TGTTTCCGTTGACGCGCAGTGGCAGGTTGTATTGCGCCGGAGTCTATCTGAATGGGCCACCATGGCCGGGATTGGGCAAA
TGAGAGCAAAACAAATTAAGCATTTTTTGGATCACCCCGACGTACAAGCACTGGCTGATTTCTTATCTACTCAGAAAGTT
GTTGGGTTCGAGCTCACAGAGTAA

Upstream 100 bases:

>100_bases
TCACGAAAGTATTTTTCAGCACTGTACGCATTTTACTCTTTTAGGCTTCGACGGGTAGCTTATTTCGCGTTTTACTTCCG
ATCACAACGGAAGGAGGCTT

Downstream 100 bases:

>100_bases
ATCCTCCTAGGGCTATAACTTAATGGTCTGAGTGCTCATATTTGAATACAGGCAGGCCAAGCCGGAATCGTAAGGCGAGT
AATCTAGCGCTAAGCCCGGT

Product: NAD-dependent DNA ligase LigB

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] B

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MNILNLKIIMFLLISNIIVVGGAWATSTCPDWPATRIAVEINALEQQLNKWSAAYHQQGHSPVTDDIYDQLQDKLRVWQS
CRGLPDKTESQPIPGKGQFLHPVAHTGLKKLKDETALTRWMAGRKNLWVQPKVDGVAVTLVYHGGKLVQLLSRGNGVKGQ
NWTEKAPFISAIPQYIANAPALLTLQGELFLLMDGHQQAKSGGVNARSTVAGALMRKSPSPLLAQVGVFIWAWPDGPTTM
KEKVALLQVMGFPFTAKYSEPVMSHLDVVQWRQFWFQAPLPFVTDGVVVRQEEEPAGRYWQATPGQWSMAWKYPPLQHIA
EVKDIHFTLGRTGKGTVVLEVLPIKIDDKWIRRVNIGSVTRWKQWDIAPGDHITLALAGHGIPRLDNVVWRVHQRNTITA
PNWDKFHQLSCFQRLPHGCEPQFLSRLIWLSGPGGLDIGGIGGGFWQELIHHELINDLVGWLLLTPEQIASIPGIGNARA
EKIYQQFQRAKQQPFSRWLLALGFPQVVSVDAQWQVVLRRSLSEWATMAGIGQMRAKQIKHFLDHPDVQALADFLSTQKV
VGFELTE

Sequences:

>Translated_567_residues
MNILNLKIIMFLLISNIIVVGGAWATSTCPDWPATRIAVEINALEQQLNKWSAAYHQQGHSPVTDDIYDQLQDKLRVWQS
CRGLPDKTESQPIPGKGQFLHPVAHTGLKKLKDETALTRWMAGRKNLWVQPKVDGVAVTLVYHGGKLVQLLSRGNGVKGQ
NWTEKAPFISAIPQYIANAPALLTLQGELFLLMDGHQQAKSGGVNARSTVAGALMRKSPSPLLAQVGVFIWAWPDGPTTM
KEKVALLQVMGFPFTAKYSEPVMSHLDVVQWRQFWFQAPLPFVTDGVVVRQEEEPAGRYWQATPGQWSMAWKYPPLQHIA
EVKDIHFTLGRTGKGTVVLEVLPIKIDDKWIRRVNIGSVTRWKQWDIAPGDHITLALAGHGIPRLDNVVWRVHQRNTITA
PNWDKFHQLSCFQRLPHGCEPQFLSRLIWLSGPGGLDIGGIGGGFWQELIHHELINDLVGWLLLTPEQIASIPGIGNARA
EKIYQQFQRAKQQPFSRWLLALGFPQVVSVDAQWQVVLRRSLSEWATMAGIGQMRAKQIKHFLDHPDVQALADFLSTQKV
VGFELTE
>Mature_567_residues
MNILNLKIIMFLLISNIIVVGGAWATSTCPDWPATRIAVEINALEQQLNKWSAAYHQQGHSPVTDDIYDQLQDKLRVWQS
CRGLPDKTESQPIPGKGQFLHPVAHTGLKKLKDETALTRWMAGRKNLWVQPKVDGVAVTLVYHGGKLVQLLSRGNGVKGQ
NWTEKAPFISAIPQYIANAPALLTLQGELFLLMDGHQQAKSGGVNARSTVAGALMRKSPSPLLAQVGVFIWAWPDGPTTM
KEKVALLQVMGFPFTAKYSEPVMSHLDVVQWRQFWFQAPLPFVTDGVVVRQEEEPAGRYWQATPGQWSMAWKYPPLQHIA
EVKDIHFTLGRTGKGTVVLEVLPIKIDDKWIRRVNIGSVTRWKQWDIAPGDHITLALAGHGIPRLDNVVWRVHQRNTITA
PNWDKFHQLSCFQRLPHGCEPQFLSRLIWLSGPGGLDIGGIGGGFWQELIHHELINDLVGWLLLTPEQIASIPGIGNARA
EKIYQQFQRAKQQPFSRWLLALGFPQVVSVDAQWQVVLRRSLSEWATMAGIGQMRAKQIKHFLDHPDVQALADFLSTQKV
VGFELTE

Specific function: Catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigB subfamily

Homologues:

Organism=Escherichia coli, GI87082305, Length=564, Percent_Identity=43.7943262411348, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI1788750, Length=523, Percent_Identity=23.7093690248566, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIGB_YERP3 (A7FCR5)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001399043.1
- ProteinModelPortal:   A7FCR5
- SMR:   A7FCR5
- STRING:   A7FCR5
- GeneID:   5388139
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_0042
- NMPDR:   fig|349747.3.peg.2495
- eggNOG:   COG0272
- HOGENOM:   HBG336755
- OMA:   NARSKVA
- ProtClustDB:   PRK08097
- BioCyc:   YPSE349747:YPSIP31758_0042-MONOMER
- HAMAP:   MF_01587
- InterPro:   IPR020923
- InterPro:   IPR004150
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- SMART:   SM00532

Pfam domain/function: PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 63624; Mature: 63624

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILNLKIIMFLLISNIIVVGGAWATSTCPDWPATRIAVEINALEQQLNKWSAAYHQQGH
CCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCC
SPVTDDIYDQLQDKLRVWQSCRGLPDKTESQPIPGKGQFLHPVAHTGLKKLKDETALTRW
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHH
MAGRKNLWVQPKVDGVAVTLVYHGGKLVQLLSRGNGVKGQNWTEKAPFISAIPQYIANAP
HCCCCCEEECCCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCC
ALLTLQGELFLLMDGHQQAKSGGVNARSTVAGALMRKSPSPLLAQVGVFIWAWPDGPTTM
EEEEECCCEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCCCHH
KEKVALLQVMGFPFTAKYSEPVMSHLDVVQWRQFWFQAPLPFVTDGVVVRQEEEPAGRYW
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCE
QATPGQWSMAWKYPPLQHIAEVKDIHFTLGRTGKGTVVLEVLPIKIDDKWIRRVNIGSVT
ECCCCCCEEEECCCCHHHHHHHHHHEEEECCCCCCEEEEEEEEEEECHHHHHEECCCCCC
RWKQWDIAPGDHITLALAGHGIPRLDNVVWRVHQRNTITAPNWDKFHQLSCFQRLPHGCE
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHCCCCCC
PQFLSRLIWLSGPGGLDIGGIGGGFWQELIHHELINDLVGWLLLTPEQIASIPGIGNARA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHH
EKIYQQFQRAKQQPFSRWLLALGFPQVVSVDAQWQVVLRRSLSEWATMAGIGQMRAKQIK
HHHHHHHHHHHHCHHHHHHHHHCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFLDHPDVQALADFLSTQKVVGFELTE
HHHCCCCHHHHHHHHHCCCCCCEEECC
>Mature Secondary Structure
MNILNLKIIMFLLISNIIVVGGAWATSTCPDWPATRIAVEINALEQQLNKWSAAYHQQGH
CCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCC
SPVTDDIYDQLQDKLRVWQSCRGLPDKTESQPIPGKGQFLHPVAHTGLKKLKDETALTRW
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHH
MAGRKNLWVQPKVDGVAVTLVYHGGKLVQLLSRGNGVKGQNWTEKAPFISAIPQYIANAP
HCCCCCEEECCCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCC
ALLTLQGELFLLMDGHQQAKSGGVNARSTVAGALMRKSPSPLLAQVGVFIWAWPDGPTTM
EEEEECCCEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCCCHH
KEKVALLQVMGFPFTAKYSEPVMSHLDVVQWRQFWFQAPLPFVTDGVVVRQEEEPAGRYW
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCE
QATPGQWSMAWKYPPLQHIAEVKDIHFTLGRTGKGTVVLEVLPIKIDDKWIRRVNIGSVT
ECCCCCCEEEECCCCHHHHHHHHHHEEEECCCCCCEEEEEEEEEEECHHHHHEECCCCCC
RWKQWDIAPGDHITLALAGHGIPRLDNVVWRVHQRNTITAPNWDKFHQLSCFQRLPHGCE
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHCCCCCC
PQFLSRLIWLSGPGGLDIGGIGGGFWQELIHHELINDLVGWLLLTPEQIASIPGIGNARA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHH
EKIYQQFQRAKQQPFSRWLLALGFPQVVSVDAQWQVVLRRSLSEWATMAGIGQMRAKQIK
HHHHHHHHHHHHCHHHHHHHHHCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFLDHPDVQALADFLSTQKVVGFELTE
HHHCCCCHHHHHHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA