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The map label for this gene is manB1 [H]

Identifier: 153948587

GI number: 153948587

Start: 3419033

End: 3420406

Strand: Reverse

Name: manB1 [H]

Synonym: YpsIP31758_3037

Alternate gene names: 153948587

Gene position: 3420406-3419033 (Counterclockwise)

Preceding gene: 153949638

Following gene: 153950685

Centisome position: 72.42

GC content: 38.79

Gene sequence:

>1374_bases
ATGCCAAAACTAACCTGTTTTAAAGCCTATGATATTCGTGGCCGATTGGATGATGAATTGAATGAAGATATAGCTTACCG
TATTGGTCGCGCGTATGGTGAGTTTGCTAAACCCAAAAAGGTTGTAGTAGGGGGGGATGTTCGATTAACCAGTGAAAGTC
TTAAGTTAGCGTTAAGTCAAGGTTTACAAGATGCAGGTACTGATGTATTAGATATTGGTTTGTCAGGTACTGAGGAGATA
TATTTTTCCACCTTCCATCTTGGGGTTGATGGTGGTATAGAAGTGACTGCCAGCCATAACCCAATAGACTACAATGGAAT
GAAGTTAGTTCGTGAAGGCTCCAGGCCTATCAGTGGAGATACAGGGTTAAGGGATATTCAGTATTTAGCTGAACAGAATG
AGTTCCCTCCAGTGGACAGTGAAAAAAGAGGTGGTTATAAAAAAATATCTATTATTAATGACTATGTTGAACACTTAATT
AACTATATTAATCCTTCTCTTTTTAAACCGTTGAAATTAGTTGTAAATACTGGCAATGGTGCTGCTGGTCATGTTATTGA
TGCTTTAGAAAAACGTTTTTCAGAATTAAAAATACCGATAACTCTGATTAAAATTCATCATGAGCCAGATGGCACATTCC
CGAATGGTATTCCGAATCCACTGTTACCAGAATGCCGCCAGGATACTACTGATGCTGTGCTGATGCATGACGCTGATATG
GGTATTGCCTTTGATGGCGATTTTGACCGTTGTTTCTTCTTTAATAATAAGGGTGAGTTTATTGAAGGCTATTATATCGT
GGGGCTTTTAGCCGAAGCTTTTCTTCAAAAAAATGCTGGAGCTAAAATTATCCATGATCCACGTTTAAGCTGGAATACTA
TTGATATAGTTAATTCTTCTGGTGGAACGCCTATAATGTCCAAAACCGGGCACGCTTTTATTAAAGAACGCATGCGAGAA
GAAGATGCGGTTTATGGTGGCGAAATGAGTGCTCATCACTACTTCCGCGACTTTGCTTATTGTGATTCAGGGATGATTCC
ATGGTTACTGATTGCTGAGCTTTTATGTGTCAAAGAAAAAAGTTTGGAGCAGTTAGTTGAAGAGCGGATGCGTATTTACC
CTGCATCTGGAGAAATTAATTCTAAGTTAACCGATCCAACCTCTGCGATAGCGCGTGTTCGTGCCGAATATGAAGTTAAG
GCTATAGCTATTGACGAAACTGATGGTATTAGTATGGAATTTGATGGGTGGCGTTTTAATTTACGCAGTTCAAATACTGA
ACCTGTTGTTCGGCTTAATGTGGAGTCACATTACAATAAGACATTAATGCATCATAAAACAGCAGAGATACTTTCTCTAT
TAAGAAAAGAATAA

Upstream 100 bases:

>100_bases
AAAGAAGATATACAAATAATGAAACTCCGTGGGCTATTCTGGCCTATGTCGTTTTTATGCAAAAACTTATCTAAACTTCC
TCAGTTTTTAAAGTGATCTT

Downstream 100 bases:

>100_bases
TTATTTTAGATGGCATTCCTAGCATTACTATATATAGGTAATACTCAATGAGTAATAAACAGGCACGAAATAACCTTGAT
AACCCAATGCCGAATAACTA

Product: phosphomannomutase

Products: NA

Alternate protein names: PMM [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MPKLTCFKAYDIRGRLDDELNEDIAYRIGRAYGEFAKPKKVVVGGDVRLTSESLKLALSQGLQDAGTDVLDIGLSGTEEI
YFSTFHLGVDGGIEVTASHNPIDYNGMKLVREGSRPISGDTGLRDIQYLAEQNEFPPVDSEKRGGYKKISIINDYVEHLI
NYINPSLFKPLKLVVNTGNGAAGHVIDALEKRFSELKIPITLIKIHHEPDGTFPNGIPNPLLPECRQDTTDAVLMHDADM
GIAFDGDFDRCFFFNNKGEFIEGYYIVGLLAEAFLQKNAGAKIIHDPRLSWNTIDIVNSSGGTPIMSKTGHAFIKERMRE
EDAVYGGEMSAHHYFRDFAYCDSGMIPWLLIAELLCVKEKSLEQLVEERMRIYPASGEINSKLTDPTSAIARVRAEYEVK
AIAIDETDGISMEFDGWRFNLRSSNTEPVVRLNVESHYNKTLMHHKTAEILSLLRKE

Sequences:

>Translated_457_residues
MPKLTCFKAYDIRGRLDDELNEDIAYRIGRAYGEFAKPKKVVVGGDVRLTSESLKLALSQGLQDAGTDVLDIGLSGTEEI
YFSTFHLGVDGGIEVTASHNPIDYNGMKLVREGSRPISGDTGLRDIQYLAEQNEFPPVDSEKRGGYKKISIINDYVEHLI
NYINPSLFKPLKLVVNTGNGAAGHVIDALEKRFSELKIPITLIKIHHEPDGTFPNGIPNPLLPECRQDTTDAVLMHDADM
GIAFDGDFDRCFFFNNKGEFIEGYYIVGLLAEAFLQKNAGAKIIHDPRLSWNTIDIVNSSGGTPIMSKTGHAFIKERMRE
EDAVYGGEMSAHHYFRDFAYCDSGMIPWLLIAELLCVKEKSLEQLVEERMRIYPASGEINSKLTDPTSAIARVRAEYEVK
AIAIDETDGISMEFDGWRFNLRSSNTEPVVRLNVESHYNKTLMHHKTAEILSLLRKE
>Mature_456_residues
PKLTCFKAYDIRGRLDDELNEDIAYRIGRAYGEFAKPKKVVVGGDVRLTSESLKLALSQGLQDAGTDVLDIGLSGTEEIY
FSTFHLGVDGGIEVTASHNPIDYNGMKLVREGSRPISGDTGLRDIQYLAEQNEFPPVDSEKRGGYKKISIINDYVEHLIN
YINPSLFKPLKLVVNTGNGAAGHVIDALEKRFSELKIPITLIKIHHEPDGTFPNGIPNPLLPECRQDTTDAVLMHDADMG
IAFDGDFDRCFFFNNKGEFIEGYYIVGLLAEAFLQKNAGAKIIHDPRLSWNTIDIVNSSGGTPIMSKTGHAFIKERMREE
DAVYGGEMSAHHYFRDFAYCDSGMIPWLLIAELLCVKEKSLEQLVEERMRIYPASGEINSKLTDPTSAIARVRAEYEVKA
IAIDETDGISMEFDGWRFNLRSSNTEPVVRLNVESHYNKTLMHHKTAEILSLLRKE

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O9 antigen [H]

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family [H]

Homologues:

Organism=Escherichia coli, GI1788361, Length=454, Percent_Identity=74.0088105726872, Blast_Score=726, Evalue=0.0,
Organism=Escherichia coli, GI1789566, Length=447, Percent_Identity=26.8456375838926, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066
- InterPro:   IPR005841 [H]

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV [H]

EC number: =5.4.2.8 [H]

Molecular weight: Translated: 51172; Mature: 51041

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00710 PGM_PMM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLTCFKAYDIRGRLDDELNEDIAYRIGRAYGEFAKPKKVVVGGDVRLTSESLKLALSQ
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEECHHHHHHHHHH
GLQDAGTDVLDIGLSGTEEIYFSTFHLGVDGGIEVTASHNPIDYNGMKLVREGSRPISGD
CHHHHCCCEEEECCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCCHHHEECCCCCCCCC
TGLRDIQYLAEQNEFPPVDSEKRGGYKKISIINDYVEHLINYINPSLFKPLKLVVNTGNG
CCHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCHHHCCCEEEEEECCCC
AAGHVIDALEKRFSELKIPITLIKIHHEPDGTFPNGIPNPLLPECRQDTTDAVLMHDADM
CCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCHHCCCCCCEEEEEECCC
GIAFDGDFDRCFFFNNKGEFIEGYYIVGLLAEAFLQKNAGAKIIHDPRLSWNTIDIVNSS
CEEECCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECC
GGTPIMSKTGHAFIKERMREEDAVYGGEMSAHHYFRDFAYCDSGMIPWLLIAELLCVKEK
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SLEQLVEERMRIYPASGEINSKLTDPTSAIARVRAEYEVKAIAIDETDGISMEFDGWRFN
HHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHCEEEEEEEEECCCCCEEEECCEEEE
LRSSNTEPVVRLNVESHYNKTLMHHKTAEILSLLRKE
ECCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKLTCFKAYDIRGRLDDELNEDIAYRIGRAYGEFAKPKKVVVGGDVRLTSESLKLALSQ
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEECHHHHHHHHHH
GLQDAGTDVLDIGLSGTEEIYFSTFHLGVDGGIEVTASHNPIDYNGMKLVREGSRPISGD
CHHHHCCCEEEECCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCCHHHEECCCCCCCCC
TGLRDIQYLAEQNEFPPVDSEKRGGYKKISIINDYVEHLINYINPSLFKPLKLVVNTGNG
CCHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCHHHCCCEEEEEECCCC
AAGHVIDALEKRFSELKIPITLIKIHHEPDGTFPNGIPNPLLPECRQDTTDAVLMHDADM
CCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCHHCCCCCCEEEEEECCC
GIAFDGDFDRCFFFNNKGEFIEGYYIVGLLAEAFLQKNAGAKIIHDPRLSWNTIDIVNSS
CEEECCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECC
GGTPIMSKTGHAFIKERMREEDAVYGGEMSAHHYFRDFAYCDSGMIPWLLIAELLCVKEK
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SLEQLVEERMRIYPASGEINSKLTDPTSAIARVRAEYEVKAIAIDETDGISMEFDGWRFN
HHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHCEEEEEEEEECCCCCEEEECCEEEE
LRSSNTEPVVRLNVESHYNKTLMHHKTAEILSLLRKE
ECCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7515042; 9004408 [H]