Click here to switch to the map view.

The map label for this gene is potB [H]

Identifier: 153948426

GI number: 153948426

Start: 1439567

End: 1440415

Strand: Direct

Name: potB [H]

Synonym: YpsIP31758_1221

Alternate gene names: 153948426

Gene position: 1439567-1440415 (Clockwise)

Preceding gene: 153948099

Following gene: 153947196

Centisome position: 30.48

GC content: 53.24

Gene sequence:

>849_bases
ATGCCGAAACCACAAAAGGCACTGAAACAAAAAGGGGCGTGGCTGCTTAGGGGGTGGAAATGGGTATTTAATTTTTACAG
CGCCGCCATTTTATTATTCCTGATCGTACCGGTATTGGTCATTGTGCCGCTGTCGTTTAATGCGGGTTCTTTTCTCAGTT
ACCCAATGGCGGGTTTTTCACTGCGTTGGTATCAGACCTTTTTCAACTCGGATGAGTGGCTGGGGGCCTTGGGCAACAGC
CTGCTGATTGCCCCGTTGGCGACCTTGCTGGCGACGGGGCTGGGGGTTTTGGCCGCCATTGGCTTGGTGCGCGGGGAGTT
TCGGGGCAAAGCGTTGGTGATGGCGATGTTGATTTCGCCAATGGTGGCCCCGGTGGTGATCATTGCTGTTGGCATGTTTT
TCTTCTTTGCCCGCTTATCACTGCTCAACAGCTACCTGGGATTAGTCTTGGCCCACGCCTTGCTTGGGGTCCCATTTGTC
GTGATCACCGTGACCGCTGTCTTGAAAAGTTATGATCAGAATCTGTCGCGGGCGGCCGCCAGCTTGGGGGCATCACAGTG
GCGGGTCTTTCGTAAGGTCACCTTACCGCTGATCGCCCCCGGTGTATTTTCAGGTGCCTTGTTTGCTTTTGCGGTTTCGT
TTGATGAAGTGGTCGTGACACTGTTTCTTGCCAGCCCGCGTCAGCGCACATTACCGATCAAAATGTTCGCCGGTATCCGT
GAAAATCTCGATCCGACTATTGCGGCGGCCGCTTCATTAATGGTTGGCGCTTCGCTGTTGTTACTGATTGTGATGGAACT
GCTCCGGCGGCGTAGTGAGCGTTTACGCCATCATCTAATATCCACATAA

Upstream 100 bases:

>100_bases
CGAGTAACCCGTACCCAGGCTGAAACCGCGATTCATTAGTTCATCTATTGATGTGTTTATCCGTTCATGAGCTTATCAAC
ACATTGTGCCGGAGGAGACG

Downstream 100 bases:

>100_bases
TCTGCCAGGGAATAAGTCGAGGGCATGGAATTGAGTGTAAACACCGGATAGGGAGGTGAATGGAGGGCAGCATGTCTGAG
ATGACCACGGCACAGGCGTT

Product: spermidine/putrescine ABC transporter permease

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MPKPQKALKQKGAWLLRGWKWVFNFYSAAILLFLIVPVLVIVPLSFNAGSFLSYPMAGFSLRWYQTFFNSDEWLGALGNS
LLIAPLATLLATGLGVLAAIGLVRGEFRGKALVMAMLISPMVAPVVIIAVGMFFFFARLSLLNSYLGLVLAHALLGVPFV
VITVTAVLKSYDQNLSRAAASLGASQWRVFRKVTLPLIAPGVFSGALFAFAVSFDEVVVTLFLASPRQRTLPIKMFAGIR
ENLDPTIAAAASLMVGASLLLLIVMELLRRRSERLRHHLIST

Sequences:

>Translated_282_residues
MPKPQKALKQKGAWLLRGWKWVFNFYSAAILLFLIVPVLVIVPLSFNAGSFLSYPMAGFSLRWYQTFFNSDEWLGALGNS
LLIAPLATLLATGLGVLAAIGLVRGEFRGKALVMAMLISPMVAPVVIIAVGMFFFFARLSLLNSYLGLVLAHALLGVPFV
VITVTAVLKSYDQNLSRAAASLGASQWRVFRKVTLPLIAPGVFSGALFAFAVSFDEVVVTLFLASPRQRTLPIKMFAGIR
ENLDPTIAAAASLMVGASLLLLIVMELLRRRSERLRHHLIST
>Mature_281_residues
PKPQKALKQKGAWLLRGWKWVFNFYSAAILLFLIVPVLVIVPLSFNAGSFLSYPMAGFSLRWYQTFFNSDEWLGALGNSL
LIAPLATLLATGLGVLAAIGLVRGEFRGKALVMAMLISPMVAPVVIIAVGMFFFFARLSLLNSYLGLVLAHALLGVPFVV
ITVTAVLKSYDQNLSRAAASLGASQWRVFRKVTLPLIAPGVFSGALFAFAVSFDEVVVTLFLASPRQRTLPIKMFAGIRE
NLDPTIAAAASLMVGASLLLLIVMELLRRRSERLRHHLIST

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=230, Percent_Identity=33.9130434782609, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1787081, Length=251, Percent_Identity=34.6613545816733, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787714, Length=226, Percent_Identity=33.1858407079646, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1786980, Length=223, Percent_Identity=31.390134529148, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788764, Length=234, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87082099, Length=227, Percent_Identity=27.7533039647577, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790464, Length=242, Percent_Identity=27.6859504132231, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30779; Mature: 30648

Theoretical pI: Translated: 11.54; Mature: 11.54

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PKPQKALKQKGAWLLRGWKWVFNFYSAAILLFLIVPVLVIVPLSFNAGSFLSYPMAGFS
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCH
LRWYQTFFNSDEWLGALGNSLLIAPLATLLATGLGVLAAIGLVRGEFRGKALVMAMLISP
HHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVAPVVIIAVGMFFFFARLSLLNSYLGLVLAHALLGVPFVVITVTAVLKSYDQNLSRAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLGASQWRVFRKVTLPLIAPGVFSGALFAFAVSFDEVVVTLFLASPRQRTLPIKMFAGIR
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
ENLDPTIAAAASLMVGASLLLLIVMELLRRRSERLRHHLIST
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]