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The map label for this gene is mlaD [H]

Identifier: 153948335

GI number: 153948335

Start: 525627

End: 526184

Strand: Direct

Name: mlaD [H]

Synonym: YpsIP31758_0450

Alternate gene names: 153948335

Gene position: 525627-526184 (Clockwise)

Preceding gene: 153950379

Following gene: 153949643

Centisome position: 11.13

GC content: 48.39

Gene sequence:

>558_bases
ATGCAAACGAAGAAAAGTGAAGTCTGGGTAGGAATGTTTATACTGATTGCTATTCTGGCAGTCGTATTTCTCTGCCTAAA
AGTTGCAGATATCAAGTCAGTGGGCAATCAACCCACTTACCGTATTTACGCGAATTTTGACAATATTGGCGGCTTAAAAA
ACCACTCTCCTGTCAAAATTGGTGGCGTAGTTGTGGGGCGGGTGGCGGAAATCACCTTGGATACCAAGAACTACACGCCA
CGCGTCGCGATAGATATTCAGCAACGCTATAACCACATCCCAGATACCAGCTCATTGGCTGTACGCACATCGGGTTTACT
GGGCGAACAATTCCTGGCACTGAATGTGGGTTTTGAAGATCCGGAAATGGGCACCAGTATTTTGAAAGATGGCGGCACCA
TTCAAGACACCAAGTCTGCATTAGTTCTGGAAGATCTGATCGGCCAGTTCCTGTACAAGAGCAGCGGTGATAGCCCGGCA
GTAAGTGAAACGGCTCCGGCAACTGAAACTGCGGCTCCACAGGCTGCGGTACCTTCTCTTCCCGCTCAACATCCTTAA

Upstream 100 bases:

>100_bases
GGGATTAGCCGGGCAACGACACGTACTGTAGTGCATTCGTCACTGGCGGTATTGGGATTGGATTTTGTGCTGACAGCACT
GATGTTTGGGAACTGAGTCG

Downstream 100 bases:

>100_bases
GAGGGCATCTGAATGTTTAAACGTCTATTCATGGTCGCATTGTTGGCCATAGCACCACTGGTTCATGCTGTCGATCAAAG
CAACCCTTATCGTCTGATGG

Product: mce family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MQTKKSEVWVGMFILIAILAVVFLCLKVADIKSVGNQPTYRIYANFDNIGGLKNHSPVKIGGVVVGRVAEITLDTKNYTP
RVAIDIQQRYNHIPDTSSLAVRTSGLLGEQFLALNVGFEDPEMGTSILKDGGTIQDTKSALVLEDLIGQFLYKSSGDSPA
VSETAPATETAAPQAAVPSLPAQHP

Sequences:

>Translated_185_residues
MQTKKSEVWVGMFILIAILAVVFLCLKVADIKSVGNQPTYRIYANFDNIGGLKNHSPVKIGGVVVGRVAEITLDTKNYTP
RVAIDIQQRYNHIPDTSSLAVRTSGLLGEQFLALNVGFEDPEMGTSILKDGGTIQDTKSALVLEDLIGQFLYKSSGDSPA
VSETAPATETAAPQAAVPSLPAQHP
>Mature_185_residues
MQTKKSEVWVGMFILIAILAVVFLCLKVADIKSVGNQPTYRIYANFDNIGGLKNHSPVKIGGVVVGRVAEITLDTKNYTP
RVAIDIQQRYNHIPDTSSLAVRTSGLLGEQFLALNVGFEDPEMGTSILKDGGTIQDTKSALVLEDLIGQFLYKSSGDSPA
VSETAPATETAAPQAAVPSLPAQHP

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG1463

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, periplasmic component

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaD family [H]

Homologues:

Organism=Escherichia coli, GI1789584, Length=173, Percent_Identity=71.6763005780347, Blast_Score=248, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003399 [H]

Pfam domain/function: PF02470 MCE [H]

EC number: NA

Molecular weight: Translated: 19782; Mature: 19782

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTKKSEVWVGMFILIAILAVVFLCLKVADIKSVGNQPTYRIYANFDNIGGLKNHSPVKI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEE
GGVVVGRVAEITLDTKNYTPRVAIDIQQRYNHIPDTSSLAVRTSGLLGEQFLALNVGFED
CCEEEEEEEEEEEECCCCCCEEEEEHHHHCCCCCCCCCEEEEECCCCCCEEEEEECCCCC
PEMGTSILKDGGTIQDTKSALVLEDLIGQFLYKSSGDSPAVSETAPATETAAPQAAVPSL
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAQHP
CCCCC
>Mature Secondary Structure
MQTKKSEVWVGMFILIAILAVVFLCLKVADIKSVGNQPTYRIYANFDNIGGLKNHSPVKI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEE
GGVVVGRVAEITLDTKNYTPRVAIDIQQRYNHIPDTSSLAVRTSGLLGEQFLALNVGFED
CCEEEEEEEEEEEECCCCCCEEEEEHHHHCCCCCCCCCEEEEECCCCCCEEEEEECCCCC
PEMGTSILKDGGTIQDTKSALVLEDLIGQFLYKSSGDSPAVSETAPATETAAPQAAVPSL
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAQHP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]