Click here to switch to the map view.

The map label for this gene is carA [H]

Identifier: 153948324

GI number: 153948324

Start: 3892954

End: 3894129

Strand: Reverse

Name: carA [H]

Synonym: YpsIP31758_3454

Alternate gene names: 153948324

Gene position: 3894129-3892954 (Counterclockwise)

Preceding gene: 153948962

Following gene: 153947267

Centisome position: 82.44

GC content: 50.6

Gene sequence:

>1176_bases
TTGATTAAGTCAGCGCTATTGGTTCTCGAAGACGGAACCCAATTCCACGGTCGGGCCATCGGGGCAGAAGGTACGGCAGT
AGGGGAAGTGGTCTTCAATACGTCGATGACCGGTTATCAAGAAATCCTCACTGATCCTTCCTACTCCCGCCAGATTGTTA
CTCTTACTTATCCTCATATCGGCAATGTCGGCACTAATGCCTCCGATGAAGAATCCTCTGCAGTACACGCCCAAGGTCTT
GTTATTCGCGACCTGCCATTGATTGCCAGCAACTACCGTAATGAAGAAGGCTTATCCGAGTATCTCAAGCGGCACAATAT
TGTGGCGATTGCCGATATCGATACTCGTAAGCTGACTCGCTTGCTGCGCGAAAAAGGAGCACAGAACGGCTGCATTATTG
TTGGGGAGTTGTCTGACGCGGCCCTGGCATTGGAAAAAGCGAAAGCATTTCCAGGCTTGAAAGGGATGGATCTGGCGAAA
GAAGTGACCACCAAAGAGATGTATCAGTGGCTGCAAGGCAGTTGGACGCTGGAAGGTGATTTACCTGCGGCGAAACAACC
AGAAAATTTGCCATTCCATGTGGTGGCTTACGATTATGGTGTGAAGCGTAATATCCTGCGTATGCTGGTTGATCGCGGTT
GCCGTCTGACCGTTGTTCCAGCGCAGACACCCGCTGAAGACGTTCTGAAACTGAATCCAGATGGCATCTTCTTATCCAAT
GGTCCTGGGGACCCAGAACCATGCGATTACGCGATCACGGCCATCAAACGCTTCCTGGAAACGGATATTCCGGTATTCGG
CATCTGTCTGGGCCACCAATTGCTGGCGCTGGCCAGTGGCGCTAAAACAGTAAAAATGAAGTTCGGTCACCATGGGGGTA
ACCATCCGGTGAAAGATCTGGATGCTAGCTGTGTGATGATTACCGCGCAAAACCATGGCTTCGCCGTTGATGAAACCTCA
TTGCCATCCAACCTGCGTACTACGCATGTCTCTTTATTTGATGGTTCTCTGCAAGGGCTTCACCGTACAGATAAAGCAGC
GTTCAGCTTCCAGGGGCATCCAGAAGCCAGCCCCGGCCCGCATGATGCCGCGCCATTGTTTGATCATTTTATCGAACTGA
TTGAGGCTTACCGAGCTTCATCCGTGAGTCTTAACCGTAGTAACAGCCATAAATAA

Upstream 100 bases:

>100_bases
TGCCAAAAATTAGCTTTGAGAGCGGTTTTTGCATTGATTTAGATCGCTAGATGTGAATTAATATGCAGATAATGTGACTG
TTTATTCCCTGGAGGATGTT

Downstream 100 bases:

>100_bases
TCAGGAGCGAATACCATGCCAAAACGTACAGATATAAAAAGCATCCTGATTCTGGGCGCAGGCCCGATTGTTATCGGCCA
GGCTTGTGAGTTTGACTACT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVGTNASDEESSAVHAQGL
VIRDLPLIASNYRNEEGLSEYLKRHNIVAIADIDTRKLTRLLREKGAQNGCIIVGELSDAALALEKAKAFPGLKGMDLAK
EVTTKEMYQWLQGSWTLEGDLPAAKQPENLPFHVVAYDYGVKRNILRMLVDRGCRLTVVPAQTPAEDVLKLNPDGIFLSN
GPGDPEPCDYAITAIKRFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDLDASCVMITAQNHGFAVDETS
LPSNLRTTHVSLFDGSLQGLHRTDKAAFSFQGHPEASPGPHDAAPLFDHFIELIEAYRASSVSLNRSNSHK

Sequences:

>Translated_391_residues
MIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVGTNASDEESSAVHAQGL
VIRDLPLIASNYRNEEGLSEYLKRHNIVAIADIDTRKLTRLLREKGAQNGCIIVGELSDAALALEKAKAFPGLKGMDLAK
EVTTKEMYQWLQGSWTLEGDLPAAKQPENLPFHVVAYDYGVKRNILRMLVDRGCRLTVVPAQTPAEDVLKLNPDGIFLSN
GPGDPEPCDYAITAIKRFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDLDASCVMITAQNHGFAVDETS
LPSNLRTTHVSLFDGSLQGLHRTDKAAFSFQGHPEASPGPHDAAPLFDHFIELIEAYRASSVSLNRSNSHK
>Mature_391_residues
MIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVGTNASDEESSAVHAQGL
VIRDLPLIASNYRNEEGLSEYLKRHNIVAIADIDTRKLTRLLREKGAQNGCIIVGELSDAALALEKAKAFPGLKGMDLAK
EVTTKEMYQWLQGSWTLEGDLPAAKQPENLPFHVVAYDYGVKRNILRMLVDRGCRLTVVPAQTPAEDVLKLNPDGIFLSN
GPGDPEPCDYAITAIKRFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDLDASCVMITAQNHGFAVDETS
LPSNLRTTHVSLFDGSLQGLHRTDKAAFSFQGHPEASPGPHDAAPLFDHFIELIEAYRASSVSLNRSNSHK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=382, Percent_Identity=39.2670157068063, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI169790915, Length=394, Percent_Identity=35.5329949238579, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI21361331, Length=394, Percent_Identity=35.5329949238579, Blast_Score=229, Evalue=4e-60,
Organism=Escherichia coli, GI1786215, Length=381, Percent_Identity=86.6141732283465, Blast_Score=696, Evalue=0.0,
Organism=Caenorhabditis elegans, GI193204318, Length=390, Percent_Identity=35.1282051282051, Blast_Score=228, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6324878, Length=405, Percent_Identity=38.7654320987654, Blast_Score=247, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6322331, Length=397, Percent_Identity=37.0277078085642, Blast_Score=232, Evalue=6e-62,
Organism=Drosophila melanogaster, GI45555749, Length=388, Percent_Identity=39.4329896907216, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24642586, Length=388, Percent_Identity=39.4329896907216, Blast_Score=233, Evalue=1e-61,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 42475; Mature: 42475

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHI
CCCCEEEEEECCCEECCEEECCCCCEEEHEEECCCCCHHHHHHCCCCCCCEEEEEECCCC
GNVGTNASDEESSAVHAQGLVIRDLPLIASNYRNEEGLSEYLKRHNIVAIADIDTRKLTR
CCCCCCCCCCCCCHHHHCCEEEECCCHHHHCCCCHHHHHHHHHHCCEEEEEECCHHHHHH
LLREKGAQNGCIIVGELSDAALALEKAKAFPGLKGMDLAKEVTTKEMYQWLQGSWTLEGD
HHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECC
LPAAKQPENLPFHVVAYDYGVKRNILRMLVDRGCRLTVVPAQTPAEDVLKLNPDGIFLSN
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCHHHHEEECCCEEEECC
GPGDPEPCDYAITAIKRFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDL
CCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
DASCVMITAQNHGFAVDETSLPSNLRTTHVSLFDGSLQGLHRTDKAAFSFQGHPEASPGP
CCCEEEEEECCCCEEEECCCCCCCCCEEEEEEECCCHHHHHHCCCCEEEECCCCCCCCCC
HDAAPLFDHFIELIEAYRASSVSLNRSNSHK
CCCHHHHHHHHHHHHHHHHCCEEECCCCCCC
>Mature Secondary Structure
MIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHI
CCCCEEEEEECCCEECCEEECCCCCEEEHEEECCCCCHHHHHHCCCCCCCEEEEEECCCC
GNVGTNASDEESSAVHAQGLVIRDLPLIASNYRNEEGLSEYLKRHNIVAIADIDTRKLTR
CCCCCCCCCCCCCHHHHCCEEEECCCHHHHCCCCHHHHHHHHHHCCEEEEEECCHHHHHH
LLREKGAQNGCIIVGELSDAALALEKAKAFPGLKGMDLAKEVTTKEMYQWLQGSWTLEGD
HHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECC
LPAAKQPENLPFHVVAYDYGVKRNILRMLVDRGCRLTVVPAQTPAEDVLKLNPDGIFLSN
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCHHHHEEECCCEEEECC
GPGDPEPCDYAITAIKRFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDL
CCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
DASCVMITAQNHGFAVDETSLPSNLRTTHVSLFDGSLQGLHRTDKAAFSFQGHPEASPGP
CCCEEEEEECCCCEEEECCCCCCCCCEEEEEEECCCHHHHHHCCCCEEEECCCCCCCCCC
HDAAPLFDHFIELIEAYRASSVSLNRSNSHK
CCCHHHHHHHHHHHHHHHHCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]