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The map label for this gene is pys2 [H]

Identifier: 153948097

GI number: 153948097

Start: 186093

End: 187898

Strand: Direct

Name: pys2 [H]

Synonym: YpsIP31758_0164

Alternate gene names: 153948097

Gene position: 186093-187898 (Clockwise)

Preceding gene: 153950523

Following gene: 153947719

Centisome position: 3.94

GC content: 48.12

Gene sequence:

>1806_bases
ATGCTTTATTACGAACCTGAATTTTTAGGTTCGCTCCGCCAGGAGGCATACAACACATACGAAATAGCCAAAACCGAGCG
AGAAGTGGCTGAACGAAATAGAGTGCAGAAGAAACCGTTTGGCTTATTTATAGAGGAAATGACGTTACAGAAAACATTGA
CGGATAGCCAAGTCGCAGAAACAGCGGCACATATTATCTTACTGGAAAGTACGCTGACGTTACTGAGGCAAGAAGAAGGG
CAGGCATGGCATCAATGTGAGCACCTTATTCGTATACGTGGCAATAAAAAATTTAAGTACCTCCACGAAGTTCACGGGCT
GAGGAAATTAGCGCAGGCTAAACAAGGAGAGATAGATGTTATTAATCAATCTATTGCCAAGTTAAAAACGGAAGAAATGG
GCCTCTTAATGCAGCAACAGCGTGTTAATGCCGATCTGGAGACAGCAAAAATCACAGAGCCGCAGCGAGTTGAGAATGCA
CGTTTAACCGTAGAAGCAGCAGAAAAGGCCGCGCGCGATCGCAGGATCAGTGAAGAGATAGCGGCAACAGAAGCAAAGCG
CCAGCGAATGGAAAATGAGCGGCTAGCAGAGCAGGAGCGGCAGCGAGTTGAAGGCACCAAACAGCAAGTATCGGAAGCAT
CATGTGCTCAACAGGCCTCAGCCTGGCAAAATCGTTTCACGTTACCGGCGCTTCAGCCGTCAGGATCAGCTCAATACAGC
TTTGCAGCGTCGGGAATGAGTGCTGTTGTGTTATCCCTGCGGGAAGCCGCCGAATTACACAATTCATTTCTGGCGGCACA
GGAGCAACTCAGTGCGATAGCGACCATTTCGGCATCAGGTTCGGTGGCGGCGATGATTGCTCTGGGGATCTACCAGACTA
AAGTGGGAGAAAGCTCCGAACGGCCACCGGGTTGGAATGTTAATCCGAAATTTGTAGGCAGTATCTCGCTGAGTGCAATG
GGGTTACCTGCGACAGAATCATTGGCATCACAGGGTGAAATGGCTCTGCCAGTGCGAATGCGGATCATTGACGCTAAAGA
TTGGATAGGTTGCACGGAGATATATGCGGTAAAAACGGGCGTGGCAGGTGTATTGCCGAAGGTCAAAGTTGGTGCAGTGC
AGTACGATGAATCAACCGGCGTTTATACTTTCACTACCGACAGCACGCCGCCCCGCACGCTGATTTTTACCCCGGCACAG
CCACCGGGAGCGGAAACCCGCCCGATATTAGCCCCACCGGGCAGCACGCCGGCTACCCTGCAGCACACGGGTGAAATGAT
CATAAAACCGGTAATAACACCGACTATACTTCCACTACCGCAGTTATATGCGCGTGATTTCCACGATTACATTATCTGGT
TCCCGGCAGATTCCGGGCTGGAGCCAGTGTATGTTTATTTAAAAAGTCCTCGTGATGAGCCGGGTGTAGTAACCGGTCAT
GGTGAGCGAATTACGGGGATCTGGCTGGCAGGCGCGGGTAAAGATATGGGGGGGCCAATTCCCGCTCAGATTGCCGATAA
ATTACGTGGTAGGCAGTTTAGTCATTTTGGTAGATTTAGGGCGGCGTTTTGGAAGGTAGTTGGGGAAGACCCAAATTTAG
TTAAATATTTTAAATCGGGTAATCTAGGAAATATTAAAAATGGTAAAGCACCATCTCCTAAAGAACGTGAGCAAGTAGGC
AGACGAGTAAAGTATGAACTTCATCATCTGAAGCCTATTAGTAAAAATGGTGCTGTTTACCACATTGAAAATATTAGAGT
ATTAACTCCGAAAAGGCATATGGATATTCATAAAGGAGTAGAATAA

Upstream 100 bases:

>100_bases
TACGTAAGGTAGAATTGCCGAAACCCCAGGGTGGGGTAAGAATATTAGGTATTCCAACGGGGGCGATAGGCTTATCCAGC
AAGCAATCCGACCACATCCC

Downstream 100 bases:

>100_bases
TATGAAAAATAAAAGTATTTGTGATTACACTGAATCTGAATTTCTAGAATTAGTAAAGGAGCTTTTTAATGTGGAAAAAA
CGACTGAAGAAGAAGATATT

Product: colicin/pyocin immunity family protein

Products: NA

Alternate protein names: Killer protein [H]

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MLYYEPEFLGSLRQEAYNTYEIAKTEREVAERNRVQKKPFGLFIEEMTLQKTLTDSQVAETAAHIILLESTLTLLRQEEG
QAWHQCEHLIRIRGNKKFKYLHEVHGLRKLAQAKQGEIDVINQSIAKLKTEEMGLLMQQQRVNADLETAKITEPQRVENA
RLTVEAAEKAARDRRISEEIAATEAKRQRMENERLAEQERQRVEGTKQQVSEASCAQQASAWQNRFTLPALQPSGSAQYS
FAASGMSAVVLSLREAAELHNSFLAAQEQLSAIATISASGSVAAMIALGIYQTKVGESSERPPGWNVNPKFVGSISLSAM
GLPATESLASQGEMALPVRMRIIDAKDWIGCTEIYAVKTGVAGVLPKVKVGAVQYDESTGVYTFTTDSTPPRTLIFTPAQ
PPGAETRPILAPPGSTPATLQHTGEMIIKPVITPTILPLPQLYARDFHDYIIWFPADSGLEPVYVYLKSPRDEPGVVTGH
GERITGIWLAGAGKDMGGPIPAQIADKLRGRQFSHFGRFRAAFWKVVGEDPNLVKYFKSGNLGNIKNGKAPSPKEREQVG
RRVKYELHHLKPISKNGAVYHIENIRVLTPKRHMDIHKGVE

Sequences:

>Translated_601_residues
MLYYEPEFLGSLRQEAYNTYEIAKTEREVAERNRVQKKPFGLFIEEMTLQKTLTDSQVAETAAHIILLESTLTLLRQEEG
QAWHQCEHLIRIRGNKKFKYLHEVHGLRKLAQAKQGEIDVINQSIAKLKTEEMGLLMQQQRVNADLETAKITEPQRVENA
RLTVEAAEKAARDRRISEEIAATEAKRQRMENERLAEQERQRVEGTKQQVSEASCAQQASAWQNRFTLPALQPSGSAQYS
FAASGMSAVVLSLREAAELHNSFLAAQEQLSAIATISASGSVAAMIALGIYQTKVGESSERPPGWNVNPKFVGSISLSAM
GLPATESLASQGEMALPVRMRIIDAKDWIGCTEIYAVKTGVAGVLPKVKVGAVQYDESTGVYTFTTDSTPPRTLIFTPAQ
PPGAETRPILAPPGSTPATLQHTGEMIIKPVITPTILPLPQLYARDFHDYIIWFPADSGLEPVYVYLKSPRDEPGVVTGH
GERITGIWLAGAGKDMGGPIPAQIADKLRGRQFSHFGRFRAAFWKVVGEDPNLVKYFKSGNLGNIKNGKAPSPKEREQVG
RRVKYELHHLKPISKNGAVYHIENIRVLTPKRHMDIHKGVE
>Mature_601_residues
MLYYEPEFLGSLRQEAYNTYEIAKTEREVAERNRVQKKPFGLFIEEMTLQKTLTDSQVAETAAHIILLESTLTLLRQEEG
QAWHQCEHLIRIRGNKKFKYLHEVHGLRKLAQAKQGEIDVINQSIAKLKTEEMGLLMQQQRVNADLETAKITEPQRVENA
RLTVEAAEKAARDRRISEEIAATEAKRQRMENERLAEQERQRVEGTKQQVSEASCAQQASAWQNRFTLPALQPSGSAQYS
FAASGMSAVVLSLREAAELHNSFLAAQEQLSAIATISASGSVAAMIALGIYQTKVGESSERPPGWNVNPKFVGSISLSAM
GLPATESLASQGEMALPVRMRIIDAKDWIGCTEIYAVKTGVAGVLPKVKVGAVQYDESTGVYTFTTDSTPPRTLIFTPAQ
PPGAETRPILAPPGSTPATLQHTGEMIIKPVITPTILPLPQLYARDFHDYIIWFPADSGLEPVYVYLKSPRDEPGVVTGH
GERITGIWLAGAGKDMGGPIPAQIADKLRGRQFSHFGRFRAAFWKVVGEDPNLVKYFKSGNLGNIKNGKAPSPKEREQVG
RRVKYELHHLKPISKNGAVYHIENIRVLTPKRHMDIHKGVE

Specific function: Causes breakdown of chromosomal DNA as well as complete inhibition of lipid synthesis in sensitive cells [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the colicin/pyosin nuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003615
- InterPro:   IPR003060
- InterPro:   IPR010715
- InterPro:   IPR016128 [H]

Pfam domain/function: PF06958 Pyocin_S [H]

EC number: NA

Molecular weight: Translated: 66659; Mature: 66659

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYYEPEFLGSLRQEAYNTYEIAKTEREVAERNRVQKKPFGLFIEEMTLQKTLTDSQVAE
CCEECHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
TAAHIILLESTLTLLRQEEGQAWHQCEHLIRIRGNKKFKYLHEVHGLRKLAQAKQGEIDV
HHHHHEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHH
INQSIAKLKTEEMGLLMQQQRVNADLETAKITEPQRVENARLTVEAAEKAARDRRISEEI
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHCCCEEEHHHHHHHHHHHHHHHHH
AATEAKRQRMENERLAEQERQRVEGTKQQVSEASCAQQASAWQNRFTLPALQPSGSAQYS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
FAASGMSAVVLSLREAAELHNSFLAAQEQLSAIATISASGSVAAMIALGIYQTKVGESSE
EHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCCCCCC
RPPGWNVNPKFVGSISLSAMGLPATESLASQGEMALPVRMRIIDAKDWIGCTEIYAVKTG
CCCCCCCCCCEEEEEEEEECCCCCHHHHHCCCCEEEEEEEEEEECCCCCCHHHHEEEHHC
VAGVLPKVKVGAVQYDESTGVYTFTTDSTPPRTLIFTPAQPPGAETRPILAPPGSTPATL
HHHCCCCEEECEEEECCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCEECCCCCCCCHH
QHTGEMIIKPVITPTILPLPQLYARDFHDYIIWFPADSGLEPVYVYLKSPRDEPGVVTGH
HHCCCCEEECCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCEEEEEEECCCCCCCEEECC
GERITGIWLAGAGKDMGGPIPAQIADKLRGRQFSHFGRFRAAFWKVVGEDPNLVKYFKSG
CCEEEEEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCC
NLGNIKNGKAPSPKEREQVGRRVKYELHHLKPISKNGAVYHIENIRVLTPKRHMDIHKGV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEECCHHHCCHHCCC
E
C
>Mature Secondary Structure
MLYYEPEFLGSLRQEAYNTYEIAKTEREVAERNRVQKKPFGLFIEEMTLQKTLTDSQVAE
CCEECHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
TAAHIILLESTLTLLRQEEGQAWHQCEHLIRIRGNKKFKYLHEVHGLRKLAQAKQGEIDV
HHHHHEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHH
INQSIAKLKTEEMGLLMQQQRVNADLETAKITEPQRVENARLTVEAAEKAARDRRISEEI
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHCCCEEEHHHHHHHHHHHHHHHHH
AATEAKRQRMENERLAEQERQRVEGTKQQVSEASCAQQASAWQNRFTLPALQPSGSAQYS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
FAASGMSAVVLSLREAAELHNSFLAAQEQLSAIATISASGSVAAMIALGIYQTKVGESSE
EHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCCCCCC
RPPGWNVNPKFVGSISLSAMGLPATESLASQGEMALPVRMRIIDAKDWIGCTEIYAVKTG
CCCCCCCCCCEEEEEEEEECCCCCHHHHHCCCCEEEEEEEEEEECCCCCCHHHHEEEHHC
VAGVLPKVKVGAVQYDESTGVYTFTTDSTPPRTLIFTPAQPPGAETRPILAPPGSTPATL
HHHCCCCEEECEEEECCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCEECCCCCCCCHH
QHTGEMIIKPVITPTILPLPQLYARDFHDYIIWFPADSGLEPVYVYLKSPRDEPGVVTGH
HHCCCCEEECCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCEEEEEEECCCCCCCEEECC
GERITGIWLAGAGKDMGGPIPAQIADKLRGRQFSHFGRFRAAFWKVVGEDPNLVKYFKSG
CCEEEEEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCC
NLGNIKNGKAPSPKEREQVGRRVKYELHHLKPISKNGAVYHIENIRVLTPKRHMDIHKGV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEECCHHHCCHHCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491711; 10984043 [H]