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The map label for this gene is holA [H]

Identifier: 153947907

GI number: 153947907

Start: 3302788

End: 3303822

Strand: Direct

Name: holA [H]

Synonym: YpsIP31758_2928

Alternate gene names: 153947907

Gene position: 3302788-3303822 (Clockwise)

Preceding gene: 153948493

Following gene: 153947377

Centisome position: 69.93

GC content: 50.14

Gene sequence:

>1035_bases
ATGATCCGGATTTACCCTGAACAACTTGTCGCGCAGCTCCATGAGGGGCTACGCGCTTGTTATCTATTGTGTGGCAATGA
ACCCTTGTTGCTACAGGAAAGTCAGGACCACATTCGTCGGGTGGCATCGCAGCACGATTTCACTGAGCATTTCAGCTTTG
CGTTGGACGCGCACACCGAATGGGAGCATATTTTTAGCCTTTGCCAGGCACTGAGTTTGTTCGCCAGCCGCCAGACACTG
CTGCTGAGTTTCCCAGACAGTGGGCTGACCGCGCCAATTAGCGAGCAGTTGGTTAAATTATCTGGCCTGTTACACCCAGA
CATTTTATTGATATTGCGGGCGAATAAGTTGACCAAAGCGCAAGAGAACAGCGCCTGGTTTAAAGCTCTCAGTAAAAATG
GGGTATTTGTCAGTTGCCAGACACCGGAACAGGCCCAGCTTCCCCGCTGGGTGAGCGCTCGCGCCAAGAGCCTCAACTTG
AACGTAGACGATGCCGCCATTCAATTACTTTGCTACTGCTATGAAGGTAACTTGCTGGCCTTATCTCAGGCGTTAGAACG
GCTCTCGCTGCTCTACCCCGACGGTAAGTTAACCCTGCCTAAAGTAGAACAAGCCGTCAACGACGCTGCGCATTTCACAC
CATACCATTGGCTTGATGCCTTGTTGATGGGGAAGAGCAAGCGGGCTTGGCATATTTTGCAGCAATTACAGCAAGAAGAC
AGTGAACCCGTTATTTTACTGCGTACCGTACAACGTGAACTGCTCCTGCTGTTAGCGCTCAAACGTCAGATGGAACAAGT
TCCATTGCGCGCATTATTCGATCAACACAAAATTTGGCAGAACCGGCGGCCCATGATGACTCAGGCACTGCAACGGCTCT
CCCTTCAGCAACTGCAACAGGCGGTTCATCTGCTCACACAGATGGAAATCCGGCTGAAACAGGATTATGGTCAATCAATC
TGGCCTGAGTTAGAAACGCTATCTATGCTGATGTGTGGAAAAACATTACCTGAGAGCTTTTTTGATGCCCATTAA

Upstream 100 bases:

>100_bases
GTGATAAACCGGTGAGTGATGCCAACGCGGCTCAGGGCTCAACCCCGACAGCCGTCAATGAAACCACTCTCGGCGAACCT
GCCGTCAGTACCTCAGCCAA

Downstream 100 bases:

>100_bases
ATCCTCGGATCATTCCCTGTATGCCTTATTCGGCGGTACTTTTGATCCCATTCATTACGGTCATCTGAAACCGGTTGAAG
CATTAGCTCAGCAAGTCGGG

Product: DNA polymerase III subunit delta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MIRIYPEQLVAQLHEGLRACYLLCGNEPLLLQESQDHIRRVASQHDFTEHFSFALDAHTEWEHIFSLCQALSLFASRQTL
LLSFPDSGLTAPISEQLVKLSGLLHPDILLILRANKLTKAQENSAWFKALSKNGVFVSCQTPEQAQLPRWVSARAKSLNL
NVDDAAIQLLCYCYEGNLLALSQALERLSLLYPDGKLTLPKVEQAVNDAAHFTPYHWLDALLMGKSKRAWHILQQLQQED
SEPVILLRTVQRELLLLLALKRQMEQVPLRALFDQHKIWQNRRPMMTQALQRLSLQQLQQAVHLLTQMEIRLKQDYGQSI
WPELETLSMLMCGKTLPESFFDAH

Sequences:

>Translated_344_residues
MIRIYPEQLVAQLHEGLRACYLLCGNEPLLLQESQDHIRRVASQHDFTEHFSFALDAHTEWEHIFSLCQALSLFASRQTL
LLSFPDSGLTAPISEQLVKLSGLLHPDILLILRANKLTKAQENSAWFKALSKNGVFVSCQTPEQAQLPRWVSARAKSLNL
NVDDAAIQLLCYCYEGNLLALSQALERLSLLYPDGKLTLPKVEQAVNDAAHFTPYHWLDALLMGKSKRAWHILQQLQQED
SEPVILLRTVQRELLLLLALKRQMEQVPLRALFDQHKIWQNRRPMMTQALQRLSLQQLQQAVHLLTQMEIRLKQDYGQSI
WPELETLSMLMCGKTLPESFFDAH
>Mature_344_residues
MIRIYPEQLVAQLHEGLRACYLLCGNEPLLLQESQDHIRRVASQHDFTEHFSFALDAHTEWEHIFSLCQALSLFASRQTL
LLSFPDSGLTAPISEQLVKLSGLLHPDILLILRANKLTKAQENSAWFKALSKNGVFVSCQTPEQAQLPRWVSARAKSLNL
NVDDAAIQLLCYCYEGNLLALSQALERLSLLYPDGKLTLPKVEQAVNDAAHFTPYHWLDALLMGKSKRAWHILQQLQQED
SEPVILLRTVQRELLLLLALKRQMEQVPLRALFDQHKIWQNRRPMMTQALQRLSLQQLQQAVHLLTQMEIRLKQDYGQSI
WPELETLSMLMCGKTLPESFFDAH

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the b

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=343, Percent_Identity=71.7201166180758, Blast_Score=485, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790 [H]

Pfam domain/function: PF06144 DNA_pol3_delta [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39489; Mature: 39489

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIYPEQLVAQLHEGLRACYLLCGNEPLLLQESQDHIRRVASQHDFTEHFSFALDAHTE
CEEECHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCHHHHHHHHHCCCCC
WEHIFSLCQALSLFASRQTLLLSFPDSGLTAPISEQLVKLSGLLHPDILLILRANKLTKA
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHHH
QENSAWFKALSKNGVFVSCQTPEQAQLPRWVSARAKSLNLNVDDAAIQLLCYCYEGNLLA
HHHHHHHHHHCCCCEEEEECCCCHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHH
LSQALERLSLLYPDGKLTLPKVEQAVNDAAHFTPYHWLDALLMGKSKRAWHILQQLQQED
HHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
SEPVILLRTVQRELLLLLALKRQMEQVPLRALFDQHKIWQNRRPMMTQALQRLSLQQLQQ
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AVHLLTQMEIRLKQDYGQSIWPELETLSMLMCGKTLPESFFDAH
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHCCCC
>Mature Secondary Structure
MIRIYPEQLVAQLHEGLRACYLLCGNEPLLLQESQDHIRRVASQHDFTEHFSFALDAHTE
CEEECHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCHHHHHHHHHCCCCC
WEHIFSLCQALSLFASRQTLLLSFPDSGLTAPISEQLVKLSGLLHPDILLILRANKLTKA
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHHH
QENSAWFKALSKNGVFVSCQTPEQAQLPRWVSARAKSLNLNVDDAAIQLLCYCYEGNLLA
HHHHHHHHHHCCCCEEEEECCCCHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHH
LSQALERLSLLYPDGKLTLPKVEQAVNDAAHFTPYHWLDALLMGKSKRAWHILQQLQQED
HHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
SEPVILLRTVQRELLLLLALKRQMEQVPLRALFDQHKIWQNRRPMMTQALQRLSLQQLQQ
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AVHLLTQMEIRLKQDYGQSIWPELETLSMLMCGKTLPESFFDAH
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8505303; 1400251; 8905232; 9278503; 3316191; 8505304; 1575709 [H]