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The map label for this gene is eptA [H]

Identifier: 153947782

GI number: 153947782

Start: 2040892

End: 2042529

Strand: Reverse

Name: eptA [H]

Synonym: YpsIP31758_1764

Alternate gene names: 153947782

Gene position: 2042529-2040892 (Counterclockwise)

Preceding gene: 153949814

Following gene: 153947956

Centisome position: 43.24

GC content: 42.37

Gene sequence:

>1638_bases
ATGAACGCTCGATATAAATTACAGTGTAACGGATTAACATTTATTCTGGCCTGTGCACTGTTTTTTACCTTCTTCCAAAA
TGCACTGTTTATTTATAAAGCCTGGTCGTTGATCCGATTCGACAATAGCGACAGCTATTTTTTTGCAGCAACCATTCCAG
TCGTGATTTTCTGTGCACTGAATATTATTTTTAGCTTATTAACTCTCCCTCTGTTACGTAAGCCTATTATTATTTTATTC
CTGTTGGGAAGTGCTGTGGCTAACTACTTTATGTTCAGCTATGGTGCTGTTATCGATGCCAATATGATGCAGAATGCCTT
TGAAACTAACTCACAGGAAGCCACCGCCCTTTTCACTCCTCGTATGGCATTGTGGCTAATAACATTAGGTATTTTGCCTG
CCATGATTGTCTGCTTTGTGCGAATTCGAGCGACTCGCCCTTGGTGGTATATGCTGGGGTTACGGGTAGCAAATATTTTA
ATGTCAATGGTAATAATTTTTCTTATCGCAGTCTTGTTCTATAAAGATTATGCGTCGCTGATCCGCAATAATAAAAATAT
CGTCAAAATGCTGACACCATCAAATTTTGTTAGCGGCAGTTTTCAATTTGCCAAACACAAGTATTTTATCAGTAACATGC
CATTGGTAAAAATAGGTGAAGACGCCCATAAGGGCCCAGTGATTAATGCACAACAAAATAAAACGCTGGTGATTTTGGTG
GTGGGTGAAACCGCCCGGTCAGAAAACTTTTCATTGGGCGGATATGCTCGGGAAACGAACCCTCGCCTGCAACAACAAGA
TATCATTTATTTCAAACATGCCTCATCCTGTGGCACTGAAACGGCGGTCTCTGTCCCCTGTATGTTCTCTAATATGCCTC
GCAAAAGTTATGACGCCTCCCTGGCGAGTCATCAGGAAGGGTTAATGGATATTATTGCGCGCGCTGGATTGAGTGTATTG
TGGCGTGAAAATGATGGTGGCTGTAAAGGCGCTTGTGACCGTATTCCACACCAAGATGTGACACAATTGCAACTGAACGC
AGATTGTCAGGATGGGGTGTGTTTAGATAATGCACTTTTATATAAACTGGATAACTATATTAATGGATTACAGAATGACG
GTGTAATCGTCTTACACCAAATGGGCAGCCACGGGCCCGCATACTATCGCCGTACCAGCCCGGAGTTGACTAAATTCACC
CCGACGTGTAATAGCAACCAGATTCAGGACTGCACCGCCCAAGAACTGGTCAATACATACGATAACTCTATTCTCTATAC
TGATGCCATGCTGGATGACACCATTGCCTTGTTGAAACAGCACAGTGACCGATTTAATACGGCGTTGGTCTATCTTTCAG
ACCATGGTGAATCACTCGGTGAAAACGGCCTCTATTTACATGGTACACCTTATGTATTTGCCCCAAGCCAGCAAACTCAT
ATCCCTTTTCTGATGTGGTTATCACCAGAATATACAAAAAATTATGGTATTGACCGTCAATGCTTATCACATAGCGCACA
ACAGGATGAAGTGTCTCAGGATAATCTCTTCCATACCCTATTAGGGATGGTAAACATTCAGACCAACGAATACTTATCAG
GCATGGATTTACTGCAAAAATGCCGGAGTCTGCAATAG

Upstream 100 bases:

>100_bases
TTGATGCAAGCGCAATCTCGCCCTGCTACCGCTAAGGTTATCTTAAGTTTCATTCGATATTCTTTAGGTAATGAATGAAC
TGTAAGGTTAAATTTTCACA

Downstream 100 bases:

>100_bases
CGATGGCTGTATCAACGAGAAGCCCATCGTGGCCTTGGACAGGATATTTTCCTCAATCAAAAAATTAATCAGATAAAACA
GTCAATTAACAATATAAAAA

Product: cell division protein

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MNARYKLQCNGLTFILACALFFTFFQNALFIYKAWSLIRFDNSDSYFFAATIPVVIFCALNIIFSLLTLPLLRKPIIILF
LLGSAVANYFMFSYGAVIDANMMQNAFETNSQEATALFTPRMALWLITLGILPAMIVCFVRIRATRPWWYMLGLRVANIL
MSMVIIFLIAVLFYKDYASLIRNNKNIVKMLTPSNFVSGSFQFAKHKYFISNMPLVKIGEDAHKGPVINAQQNKTLVILV
VGETARSENFSLGGYARETNPRLQQQDIIYFKHASSCGTETAVSVPCMFSNMPRKSYDASLASHQEGLMDIIARAGLSVL
WRENDGGCKGACDRIPHQDVTQLQLNADCQDGVCLDNALLYKLDNYINGLQNDGVIVLHQMGSHGPAYYRRTSPELTKFT
PTCNSNQIQDCTAQELVNTYDNSILYTDAMLDDTIALLKQHSDRFNTALVYLSDHGESLGENGLYLHGTPYVFAPSQQTH
IPFLMWLSPEYTKNYGIDRQCLSHSAQQDEVSQDNLFHTLLGMVNIQTNEYLSGMDLLQKCRSLQ

Sequences:

>Translated_545_residues
MNARYKLQCNGLTFILACALFFTFFQNALFIYKAWSLIRFDNSDSYFFAATIPVVIFCALNIIFSLLTLPLLRKPIIILF
LLGSAVANYFMFSYGAVIDANMMQNAFETNSQEATALFTPRMALWLITLGILPAMIVCFVRIRATRPWWYMLGLRVANIL
MSMVIIFLIAVLFYKDYASLIRNNKNIVKMLTPSNFVSGSFQFAKHKYFISNMPLVKIGEDAHKGPVINAQQNKTLVILV
VGETARSENFSLGGYARETNPRLQQQDIIYFKHASSCGTETAVSVPCMFSNMPRKSYDASLASHQEGLMDIIARAGLSVL
WRENDGGCKGACDRIPHQDVTQLQLNADCQDGVCLDNALLYKLDNYINGLQNDGVIVLHQMGSHGPAYYRRTSPELTKFT
PTCNSNQIQDCTAQELVNTYDNSILYTDAMLDDTIALLKQHSDRFNTALVYLSDHGESLGENGLYLHGTPYVFAPSQQTH
IPFLMWLSPEYTKNYGIDRQCLSHSAQQDEVSQDNLFHTLLGMVNIQTNEYLSGMDLLQKCRSLQ
>Mature_545_residues
MNARYKLQCNGLTFILACALFFTFFQNALFIYKAWSLIRFDNSDSYFFAATIPVVIFCALNIIFSLLTLPLLRKPIIILF
LLGSAVANYFMFSYGAVIDANMMQNAFETNSQEATALFTPRMALWLITLGILPAMIVCFVRIRATRPWWYMLGLRVANIL
MSMVIIFLIAVLFYKDYASLIRNNKNIVKMLTPSNFVSGSFQFAKHKYFISNMPLVKIGEDAHKGPVINAQQNKTLVILV
VGETARSENFSLGGYARETNPRLQQQDIIYFKHASSCGTETAVSVPCMFSNMPRKSYDASLASHQEGLMDIIARAGLSVL
WRENDGGCKGACDRIPHQDVTQLQLNADCQDGVCLDNALLYKLDNYINGLQNDGVIVLHQMGSHGPAYYRRTSPELTKFT
PTCNSNQIQDCTAQELVNTYDNSILYTDAMLDDTIALLKQHSDRFNTALVYLSDHGESLGENGLYLHGTPYVFAPSQQTH
IPFLMWLSPEYTKNYGIDRQCLSHSAQQDEVSQDNLFHTLLGMVNIQTNEYLSGMDLLQKCRSLQ

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=531, Percent_Identity=51.0357815442561, Blast_Score=564, Evalue=1e-162,
Organism=Escherichia coli, GI87082286, Length=443, Percent_Identity=25.2821670428894, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1790392, Length=518, Percent_Identity=24.1312741312741, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61443; Mature: 61443

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARYKLQCNGLTFILACALFFTFFQNALFIYKAWSLIRFDNSDSYFFAATIPVVIFCAL
CCCEEEEEECCHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCEEEEHHHHHHHHHHH
NIIFSLLTLPLLRKPIIILFLLGSAVANYFMFSYGAVIDANMMQNAFETNSQEATALFTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEHHHHHHHHHCCCCCCEEEECH
RMALWLITLGILPAMIVCFVRIRATRPWWYMLGLRVANILMSMVIIFLIAVLFYKDYASL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRNNKNIVKMLTPSNFVSGSFQFAKHKYFISNMPLVKIGEDAHKGPVINAQQNKTLVILV
HHCCCCEEEEECCCCCCCCCHHHHHHEEEECCCCEEEECCCCCCCCEEECCCCCEEEEEE
VGETARSENFSLGGYARETNPRLQQQDIIYFKHASSCGTETAVSVPCMFSNMPRKSYDAS
ECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEECCHHHCCCCCCHHHHH
LASHQEGLMDIIARAGLSVLWRENDGGCKGACDRIPHQDVTQLQLNADCQDGVCLDNALL
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCCCCCEEEECCCCCCCEEHHHHHH
YKLDNYINGLQNDGVIVLHQMGSHGPAYYRRTSPELTKFTPTCNSNQIQDCTAQELVNTY
HHHHHHHCCCCCCCEEEEEECCCCCCCCEECCCCCCEEECCCCCCCCCCHHHHHHHHHHH
DNSILYTDAMLDDTIALLKQHSDRFNTALVYLSDHGESLGENGLYLHGTPYVFAPSQQTH
CCCEEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCEEECCCCCCC
IPFLMWLSPEYTKNYGIDRQCLSHSAQQDEVSQDNLFHTLLGMVNIQTNEYLSGMDLLQK
CCEEEEECCCHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
CRSLQ
HHCCC
>Mature Secondary Structure
MNARYKLQCNGLTFILACALFFTFFQNALFIYKAWSLIRFDNSDSYFFAATIPVVIFCAL
CCCEEEEEECCHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCEEEEHHHHHHHHHHH
NIIFSLLTLPLLRKPIIILFLLGSAVANYFMFSYGAVIDANMMQNAFETNSQEATALFTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEHHHHHHHHHCCCCCCEEEECH
RMALWLITLGILPAMIVCFVRIRATRPWWYMLGLRVANILMSMVIIFLIAVLFYKDYASL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRNNKNIVKMLTPSNFVSGSFQFAKHKYFISNMPLVKIGEDAHKGPVINAQQNKTLVILV
HHCCCCEEEEECCCCCCCCCHHHHHHEEEECCCCEEEECCCCCCCCEEECCCCCEEEEEE
VGETARSENFSLGGYARETNPRLQQQDIIYFKHASSCGTETAVSVPCMFSNMPRKSYDAS
ECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEECCHHHCCCCCCHHHHH
LASHQEGLMDIIARAGLSVLWRENDGGCKGACDRIPHQDVTQLQLNADCQDGVCLDNALL
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCCCCCEEEECCCCCCCEEHHHHHH
YKLDNYINGLQNDGVIVLHQMGSHGPAYYRRTSPELTKFTPTCNSNQIQDCTAQELVNTY
HHHHHHHCCCCCCCEEEEEECCCCCCCCEECCCCCCEEECCCCCCCCCCHHHHHHHHHHH
DNSILYTDAMLDDTIALLKQHSDRFNTALVYLSDHGESLGENGLYLHGTPYVFAPSQQTH
CCCEEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCEEECCCCCCC
IPFLMWLSPEYTKNYGIDRQCLSHSAQQDEVSQDNLFHTLLGMVNIQTNEYLSGMDLLQK
CCEEEEECCCHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
CRSLQ
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]