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The map label for this gene is rpoH [H]

Identifier: 153947554

GI number: 153947554

Start: 271277

End: 272134

Strand: Direct

Name: rpoH [H]

Synonym: YpsIP31758_0242

Alternate gene names: 153947554

Gene position: 271277-272134 (Clockwise)

Preceding gene: 153949919

Following gene: 153949569

Centisome position: 5.74

GC content: 50.35

Gene sequence:

>858_bases
ATGACCAAAGAAATGCAAACTTTAGCCTTAGTGCCCCAGGGTAGTCTGGAAGCCTATATTCGGGCTGCCAACGCCTATCC
AATGCTGACTGCAGAGGAAGAACGGGAGCTGGCTGAACGGCTGCATTACCAGGGCGATCTGGGAGCGGCTAAACAGCTTA
TCCTGTCTCACCTGCGCTTTGTTGCTCATGTTGCCCGCAACTATTCCGGGTACGGTTTGCCACAGGCTGACCTTATTCAG
GAAGGTAATATTGGCTTGATGAAAGCGGTTCGCCGTTTTAACCCTGAAGTCGGGGTGCGTCTGGTGTCCTTCGCTGTGCA
CTGGATCAAAGCAGAAATTCATGAATATGTTCTGCGTAACTGGCGGATTGTGAAAGTCGCAACTACCAAAGCTCAGCGTA
AGCTGTTCTTCAACCTGCGTAAAACCAAGCAGCGCCTGGGCTGGTTTAATCAGGATGAAGTAGAATTGGTCGCCAAAGAG
CTTGGCGTAACCAGTAAAGACGTTCGCGAGATGGAGTCACGTATGTCCGCTCAGGATATGACGTTCGATCCTTCTCCTGA
TGACGAAGCACGTGACGGCCAATTCATGGCTCCAGTCCTGTATTTGCAGGATAAAACCTCTGATTTTGCTGATGGCATCG
AAGAGGATAACTGGGATACCCACGCGGCAGATAAACTGTCTTATGCGTTGGAAGGGTTGGACGAGCGGAGCCAACATATT
ATTCGCGCCCGTTGGCTGGACGATGAGAACAAGTCCACCTTACAAGAACTGGCCGATCAGTACGGTGTGTCTGCTGAACG
TGTACGTCAGCTTGAAAAGAACGCGATGAAAAAATTGCGTATGGCGATTGAAGCCTAA

Upstream 100 bases:

>100_bases
CAAAATGATAAGTTGCTCAGCGCTTGAATCTGTATAACCAGGTGCTAAAATATTAGCGCTGAGAGAAACCTGCACTAAAC
ATGCAACTGAGAGGGTTTAA

Downstream 100 bases:

>100_bases
TAGCAACAAACAGATGCTGTAATCCGGCATGATAAAGAAGGGCGATATTGATTATCGCCCTTTTGCTTTTTGGTAGATAT
GGACGATTACCGTTTGGTAG

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTKEMQTLALVPQGSLEAYIRAANAYPMLTAEEERELAERLHYQGDLGAAKQLILSHLRFVAHVARNYSGYGLPQADLIQ
EGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVELVAKE
LGVTSKDVREMESRMSAQDMTFDPSPDDEARDGQFMAPVLYLQDKTSDFADGIEEDNWDTHAADKLSYALEGLDERSQHI
IRARWLDDENKSTLQELADQYGVSAERVRQLEKNAMKKLRMAIEA

Sequences:

>Translated_285_residues
MTKEMQTLALVPQGSLEAYIRAANAYPMLTAEEERELAERLHYQGDLGAAKQLILSHLRFVAHVARNYSGYGLPQADLIQ
EGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVELVAKE
LGVTSKDVREMESRMSAQDMTFDPSPDDEARDGQFMAPVLYLQDKTSDFADGIEEDNWDTHAADKLSYALEGLDERSQHI
IRARWLDDENKSTLQELADQYGVSAERVRQLEKNAMKKLRMAIEA
>Mature_284_residues
TKEMQTLALVPQGSLEAYIRAANAYPMLTAEEERELAERLHYQGDLGAAKQLILSHLRFVAHVARNYSGYGLPQADLIQE
GNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVELVAKEL
GVTSKDVREMESRMSAQDMTFDPSPDDEARDGQFMAPVLYLQDKTSDFADGIEEDNWDTHAADKLSYALEGLDERSQHII
RARWLDDENKSTLQELADQYGVSAERVRQLEKNAMKKLRMAIEA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=285, Percent_Identity=83.5087719298246, Blast_Score=483, Evalue=1e-138,
Organism=Escherichia coli, GI1789098, Length=281, Percent_Identity=28.1138790035587, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789448, Length=241, Percent_Identity=29.4605809128631, Blast_Score=88, Evalue=8e-19,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32715; Mature: 32583

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEMQTLALVPQGSLEAYIRAANAYPMLTAEEERELAERLHYQGDLGAAKQLILSHLRF
CCCCCCCEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VAHVARNYSGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVELVAKELGVTSKDVREMESRMSAQDM
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
TFDPSPDDEARDGQFMAPVLYLQDKTSDFADGIEEDNWDTHAADKLSYALEGLDERSQHI
CCCCCCCCCCCCCCEECCEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IRARWLDDENKSTLQELADQYGVSAERVRQLEKNAMKKLRMAIEA
HHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKEMQTLALVPQGSLEAYIRAANAYPMLTAEEERELAERLHYQGDLGAAKQLILSHLRF
CCCCCCEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VAHVARNYSGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVELVAKELGVTSKDVREMESRMSAQDM
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
TFDPSPDDEARDGQFMAPVLYLQDKTSDFADGIEEDNWDTHAADKLSYALEGLDERSQHI
CCCCCCCCCCCCCCEECCEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IRARWLDDENKSTLQELADQYGVSAERVRQLEKNAMKKLRMAIEA
HHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]