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The map label for this gene is fliA2 [H]

Identifier: 153947529

GI number: 153947529

Start: 767618

End: 768313

Strand: Direct

Name: fliA2 [H]

Synonym: YpsIP31758_0660

Alternate gene names: 153947529

Gene position: 767618-768313 (Clockwise)

Preceding gene: 153947631

Following gene: 153947051

Centisome position: 16.25

GC content: 45.11

Gene sequence:

>696_bases
ATGGAAAAAAGAGAATTTGAAGCGTGCTTAACGCTCACTGCCGCAGAGGAAGGGAAATATCTGAACGCGTATTTACCGCT
GGTAAAACGTATTGTCAGGCAGCTCTCTTTCCAGGCGGATAGCGTCATCGGCAAGGAGGATATGCAGCAAATTGCATTGA
TGGGGCTATTAGAGGCACTGCGCCGCTATGGTCACCCTGACGGGCAATTTGCCGCTTATGCTGTTCATCGGATCCGCGGT
TCAATATTGGATCAATTGAGGGAGCATGATTGGCGGCCCCGCCGGTTGCGGCAGAAAACCCACAAAACCAATGAGGCCAT
CCGGCAAATCGCGAAACGGCTGGGCCATGAACCTAACTTTGAGGAGATCAGCGCCGAATTAAATCTAACGGCAGATGAAT
ACCAGGAATATCTCTTGTTGGAGAATGCGGGTGCGATGGAAAGCCTGGATGAAATTCTCGCATTAGATGCTCACAATGAT
ATTTTGCAAAGTCGTGATCTGGAAGAGAATATTATTATTGAAGATAATCTCAAACAGGCGATCAGCGCATTAGATGAACG
TGAGCAAATGATTTTGCATCTTTATTATCAGCAAGAACTGAGTATGAAAGAGATTGCACAAGTACTGGATCTCACTGAGG
CACGCATCTGCCAGCTCAATAAGAAATTAGTACAAAAAATTAAATCATTTTTTTAA

Upstream 100 bases:

>100_bases
GCCAGCTAATGAATGAATATAAGCAAGATTTCGCAGAATTAAATGTGCCGATGCCATTTGATGATGTGGTTATCAGCAGA
ATGGTCTTTCAATAATCATA

Downstream 100 bases:

>100_bases
ATTATCGAGGGTGTTATGCAGAAGATATTTGGCTTGCTGGTCATTATGGTATGTGTGATTGGTGGCTATTTAATGTCTGG
CGGAGTGCTATCGTCATTTT

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-F factor [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MEKREFEACLTLTAAEEGKYLNAYLPLVKRIVRQLSFQADSVIGKEDMQQIALMGLLEALRRYGHPDGQFAAYAVHRIRG
SILDQLREHDWRPRRLRQKTHKTNEAIRQIAKRLGHEPNFEEISAELNLTADEYQEYLLLENAGAMESLDEILALDAHND
ILQSRDLEENIIIEDNLKQAISALDEREQMILHLYYQQELSMKEIAQVLDLTEARICQLNKKLVQKIKSFF

Sequences:

>Translated_231_residues
MEKREFEACLTLTAAEEGKYLNAYLPLVKRIVRQLSFQADSVIGKEDMQQIALMGLLEALRRYGHPDGQFAAYAVHRIRG
SILDQLREHDWRPRRLRQKTHKTNEAIRQIAKRLGHEPNFEEISAELNLTADEYQEYLLLENAGAMESLDEILALDAHND
ILQSRDLEENIIIEDNLKQAISALDEREQMILHLYYQQELSMKEIAQVLDLTEARICQLNKKLVQKIKSFF
>Mature_231_residues
MEKREFEACLTLTAAEEGKYLNAYLPLVKRIVRQLSFQADSVIGKEDMQQIALMGLLEALRRYGHPDGQFAAYAVHRIRG
SILDQLREHDWRPRRLRQKTHKTNEAIRQIAKRLGHEPNFEEISAELNLTADEYQEYLLLENAGAMESLDEILALDAHND
ILQSRDLEENIIIEDNLKQAISALDEREQMILHLYYQQELSMKEIAQVLDLTEARICQLNKKLVQKIKSFF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=217, Percent_Identity=33.6405529953917, Blast_Score=120, Evalue=6e-29,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 26891; Mature: 26891

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKREFEACLTLTAAEEGKYLNAYLPLVKRIVRQLSFQADSVIGKEDMQQIALMGLLEAL
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RRYGHPDGQFAAYAVHRIRGSILDQLREHDWRPRRLRQKTHKTNEAIRQIAKRLGHEPNF
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EEISAELNLTADEYQEYLLLENAGAMESLDEILALDAHNDILQSRDLEENIIIEDNLKQA
HHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCEEHHHHHHH
ISALDEREQMILHLYYQQELSMKEIAQVLDLTEARICQLNKKLVQKIKSFF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKREFEACLTLTAAEEGKYLNAYLPLVKRIVRQLSFQADSVIGKEDMQQIALMGLLEAL
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RRYGHPDGQFAAYAVHRIRGSILDQLREHDWRPRRLRQKTHKTNEAIRQIAKRLGHEPNF
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EEISAELNLTADEYQEYLLLENAGAMESLDEILALDAHNDILQSRDLEENIIIEDNLKQA
HHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCEEHHHHHHH
ISALDEREQMILHLYYQQELSMKEIAQVLDLTEARICQLNKKLVQKIKSFF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501040; 12620739 [H]