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The map label for this gene is proP [H]

Identifier: 153947347

GI number: 153947347

Start: 3911987

End: 3913366

Strand: Reverse

Name: proP [H]

Synonym: YpsIP31758_3469

Alternate gene names: 153947347

Gene position: 3913366-3911987 (Counterclockwise)

Preceding gene: 153946905

Following gene: 153949194

Centisome position: 82.85

GC content: 46.16

Gene sequence:

>1380_bases
ATGTCTCAAGATCACAATTCATCTCAAGTCGGCTCATCCCAAATCGACTCATCTCAACATCATGTTGATCAACAACATAA
TCATAATCGTCGATTGAATAAGCAAGATTACAAAACACTCACCTTAGCCGCATTAGGTGGAGCGTTAGAATTCTATGATT
TTATTATTTTTGTCTTCTTTGCTGCGGTTATTGGTGATCTTTTCTTCCCGGCTGATATGCCTGAATGGCTTCGTCAGGTG
CAGACGTTCGGTATTTTTGCTGCGGGTTACTTGGCTCGCCCATTGGGTGGGATCATCATGGCACATTTTGGTGATTTGGT
TGGGCGTAAAAAGATGTTTACTCTTAGCATCTTATTAATGGCGTTGCCAACACTGGCTATTGGCATGTTGCCGACATATG
CCACTATCGGGATCACCGCACCTTTACTTCTACTATTAATGCGGGTATTACAAGGTGCTGCCATTGGTGGCGAAGTTCCC
GGTGCATGGGTCTTTGTGGCAGAACATGTGCCACGTAAACGTATTGGCATTGCCTGCGGTACCTTAACGGCCGGTCTGAC
TGCTGGGATTTTGTTAGGATCGCTGGTTGCTACAGTCATGAATACTACGCTGGGCCATCAGGCGATTTTGGAGGGGGGAT
GGCGTATACCGTTCTTCTTGGGCGGAATTTTCGGTTTGTTTGCCATGTATTTACGCCGTTGGTTGCAGGAAACTCCGATC
TTTAAAGAAATGCAGGCGCGTAAAACATTGGCTGAAGAATTGCCGCTAAAATCGGTGGTGGTAAACCATAAGAAAGAAGT
GGTTGTTTCGATGCTGCTGACTTGGTTGCTCTCTGCTGGCATCGTCGTCGTTATTTTGATGACACCAACCTATCTACAGA
AGCAGTTTAATGTACTGCCAGAGTTGGCATTGCAGGCAAACAGTTTGGCGATTATCGCATTGGTTATCGGCTGTGTGGTT
GCCGGATTGGCAATTGATCGCTTTGGGGCCAGTAAAACCTTTATCGTTGGCAGCCTGATGCTGGCGATGTCGACATGGTC
GTTCTATCACACCAATCTGACCAATCCAGCTCAGTTATTTCCCCACTATATGTTGGCTGGCTTCTGTGTCGGTATCGTTG
GTGCCGTGCCTTATGTCATGGTGCGAGCCTTTCCGGCAGAAGTCCGCTTCACAGGCATCTCTTTCTCGTACAATGTTGCT
TATGCCATTTTTGGTGGTTTAACACCTATTCTGGTGACCTTATGGATGAAGTCATCTGCAATGGCTCCGGCGTATTACAT
GTTGGTACTATCACTGGTGGGATTGTTATTGGGTATTTATCTGCGTAACGACATTAATAGTGAAGTGAAGGTTCAGATGC
CTAAGAGGGTGATTAACTGA

Upstream 100 bases:

>100_bases
CATTATGTGCGTGTGGGGAATTTTGTACGGTATAGTCATGCTAACTTTACAAAGAGTAGAAAAATTTTTCTTCCCCAACT
CTGGCTTATAACGGTGTCAT

Downstream 100 bases:

>100_bases
GTTAATTAAGTCAATTCGTCTTAAAAAGCCCATAATTATTATCAATAAATTATGGGCTTTTATCTTTTTATCTATACTTT
AGAATTCTGATTTAAAGGTA

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSQDHNSSQVGSSQIDSSQHHVDQQHNHNRRLNKQDYKTLTLAALGGALEFYDFIIFVFFAAVIGDLFFPADMPEWLRQV
QTFGIFAAGYLARPLGGIIMAHFGDLVGRKKMFTLSILLMALPTLAIGMLPTYATIGITAPLLLLLMRVLQGAAIGGEVP
GAWVFVAEHVPRKRIGIACGTLTAGLTAGILLGSLVATVMNTTLGHQAILEGGWRIPFFLGGIFGLFAMYLRRWLQETPI
FKEMQARKTLAEELPLKSVVVNHKKEVVVSMLLTWLLSAGIVVVILMTPTYLQKQFNVLPELALQANSLAIIALVIGCVV
AGLAIDRFGASKTFIVGSLMLAMSTWSFYHTNLTNPAQLFPHYMLAGFCVGIVGAVPYVMVRAFPAEVRFTGISFSYNVA
YAIFGGLTPILVTLWMKSSAMAPAYYMLVLSLVGLLLGIYLRNDINSEVKVQMPKRVIN

Sequences:

>Translated_459_residues
MSQDHNSSQVGSSQIDSSQHHVDQQHNHNRRLNKQDYKTLTLAALGGALEFYDFIIFVFFAAVIGDLFFPADMPEWLRQV
QTFGIFAAGYLARPLGGIIMAHFGDLVGRKKMFTLSILLMALPTLAIGMLPTYATIGITAPLLLLLMRVLQGAAIGGEVP
GAWVFVAEHVPRKRIGIACGTLTAGLTAGILLGSLVATVMNTTLGHQAILEGGWRIPFFLGGIFGLFAMYLRRWLQETPI
FKEMQARKTLAEELPLKSVVVNHKKEVVVSMLLTWLLSAGIVVVILMTPTYLQKQFNVLPELALQANSLAIIALVIGCVV
AGLAIDRFGASKTFIVGSLMLAMSTWSFYHTNLTNPAQLFPHYMLAGFCVGIVGAVPYVMVRAFPAEVRFTGISFSYNVA
YAIFGGLTPILVTLWMKSSAMAPAYYMLVLSLVGLLLGIYLRNDINSEVKVQMPKRVIN
>Mature_458_residues
SQDHNSSQVGSSQIDSSQHHVDQQHNHNRRLNKQDYKTLTLAALGGALEFYDFIIFVFFAAVIGDLFFPADMPEWLRQVQ
TFGIFAAGYLARPLGGIIMAHFGDLVGRKKMFTLSILLMALPTLAIGMLPTYATIGITAPLLLLLMRVLQGAAIGGEVPG
AWVFVAEHVPRKRIGIACGTLTAGLTAGILLGSLVATVMNTTLGHQAILEGGWRIPFFLGGIFGLFAMYLRRWLQETPIF
KEMQARKTLAEELPLKSVVVNHKKEVVVSMLLTWLLSAGIVVVILMTPTYLQKQFNVLPELALQANSLAIIALVIGCVVA
GLAIDRFGASKTFIVGSLMLAMSTWSFYHTNLTNPAQLFPHYMLAGFCVGIVGAVPYVMVRAFPAEVRFTGISFSYNVAY
AIFGGLTPILVTLWMKSSAMAPAYYMLVLSLVGLLLGIYLRNDINSEVKVQMPKRVIN

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=433, Percent_Identity=32.5635103926097, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI1788292, Length=400, Percent_Identity=31.5, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1789941, Length=416, Percent_Identity=30.2884615384615, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788942, Length=429, Percent_Identity=31.2354312354312, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082231, Length=478, Percent_Identity=24.2677824267782, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87082404, Length=392, Percent_Identity=22.1938775510204, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 50216; Mature: 50085

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHCC
>Mature Secondary Structure 
SQDHNSSQVGSSQIDSSQHHVDQQHNHNRRLNKQDYKTLTLAALGGALEFYDFIIFVFF
CCCCCCCCCCCHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH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CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]