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The map label for this gene is nadB

Identifier: 153947342

GI number: 153947342

Start: 1328642

End: 1330243

Strand: Reverse

Name: nadB

Synonym: YpsIP31758_1128

Alternate gene names: 153947342

Gene position: 1330243-1328642 (Counterclockwise)

Preceding gene: 153947661

Following gene: 153950343

Centisome position: 28.16

GC content: 48.81

Gene sequence:

>1602_bases
ATGCAATCATCATCTGAACACATTAGCGATGTCTTGATCATCGGGAGTGGCGCTGCTGGCTTGTCTTTAGCTCTCCGCCT
CGCGCCACATTGTAAAGTTACCGTTCTGAGCAAAGGGCCTCTTAATGAGGGAGCCACTTTTTATGCTCAGGGAGGTATTG
CCGCTGTTTTCGACGAAACTGACAGTATCAGTTCACATGTCGATGACACCTTAATCGCCGGTGCCGGGCTTTGTGACAAA
GAAGCCGTTGAATTTATTGCCAGCAATGCCCGCTCTTGTGTGCAGTGGCTCATTGATCAAGGCGTTCTTTTTGATACAGA
GACCAGTGCTAACGGTGAAGAGCGCTATCACCTTACCCGTGAAGGTGGTCATAGCCACCGCCGTATTCTGCATACAGCGG
ATGCTACCGGTAAAGAAGTAGAAACTACCTTAGTGGGTAAAGCAAGCAATCACCCTAATATTTCAGTGAAAGAACGCTGC
AATGCTGTCGATCTTATCACATCAAACAAAATTGGTTTGGCGGGTACCAAACGCGTAGTGGGTGCTTATGTCTGGAACCG
TGAGTTAGAGAAAGTTGAGACTTTCCGCGCCAAAGCCGTGGTTTTGGCGACAGGCGGCGCGGCTAAAGTTTATCAATACA
CCACTAACCCAGATATTTCGTCTGGTGATGGTATTGCCATGGCGTGGCGTGCCGGGTGCCGGGTCGCTAATTTGGAATTT
AACCAATTTCATCCAACCTGTCTCTTCCACCCGCAAGCACGTAATTTTTTACTGACAGAAGCCTTACGTGGTGAAGGTGC
CTACCTTAAACGCCCTGATGGCAGCCGTTTCATGCTTGATTTTGATCCACGCGGTGAACTTGCGCCACGTGATATCGTTG
CCCGAGCCATCGACCATGAAATGAAGCGCCTCGGTGCTGATTGCATGTATCTGGATATCAGCCATAAACCTACTGATTTC
GTGATGCAACATTTCCCAATGATCTATGAAAAACTGCTGTCATTGGGTATCGATCTAACCAAAGAAGCTATTCCGATTGT
CCCCGCAGCTCATTACACCTGTGGTGGCGTAATGGTTGATCAACATGGGCGTACCGATTTAGATGGGTTATATGCGATTG
GTGAAGTGAGCTACACCGGCTTACACGGTGCCAACCGCATGGCCTCCAATTCCCTGCTCGAATGCCTGGTCTATGGCTGG
TCAGCAGCAGAAGACATTCTGACACGGCTGCCGACGGCCAAACTGGCCAAACATCTGCCAGATTGGGATGAAAGCCGCGT
TGATAATTCTGACGAACGGGTTGTCATTCAGCATAACTGGCATGAGTTGCGTTTATTTATGTGGGATTACGTCGGCATTG
TCCGTACCACAAAACGCTTAGAGCGGGCTCTACGGCGCATTAACACGTTGCAGCAAGAGATTGATGAATATTATGCCAAT
TTCCGTATCTCCAATAACCTGCTGGAGTTACGGAATCTGGTACAAGTCGCGGAGTTAATCGTGCGCTGTGCGATGGAACG
TAAAGAGAGCCGTGGGTTGCACTATACGCTTGATTACCCTGATCAAATAGAAAACCCACAGCCAACCATTCTGCATCCAT
AA

Upstream 100 bases:

>100_bases
TTTTTGTCCAAATATTATTCAGACATCTTATACCCATAGCTTTTTCTTGAGCAGATAGGCTAACATGAAACCAACTCTTC
TTCTGCACACATCATACGAT

Downstream 100 bases:

>100_bases
CACATTCTGAATCCATAAGATTGAGGGCGTCTCTACGACGCCCGCAGACTGCTGACAAACCCCGATGACGCGATCTTGAA
CGGTGAGGATAGGCAGAGGA

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MQSSSEHISDVLIIGSGAAGLSLALRLAPHCKVTVLSKGPLNEGATFYAQGGIAAVFDETDSISSHVDDTLIAGAGLCDK
EAVEFIASNARSCVQWLIDQGVLFDTETSANGEERYHLTREGGHSHRRILHTADATGKEVETTLVGKASNHPNISVKERC
NAVDLITSNKIGLAGTKRVVGAYVWNRELEKVETFRAKAVVLATGGAAKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
NQFHPTCLFHPQARNFLLTEALRGEGAYLKRPDGSRFMLDFDPRGELAPRDIVARAIDHEMKRLGADCMYLDISHKPTDF
VMQHFPMIYEKLLSLGIDLTKEAIPIVPAAHYTCGGVMVDQHGRTDLDGLYAIGEVSYTGLHGANRMASNSLLECLVYGW
SAAEDILTRLPTAKLAKHLPDWDESRVDNSDERVVIQHNWHELRLFMWDYVGIVRTTKRLERALRRINTLQQEIDEYYAN
FRISNNLLELRNLVQVAELIVRCAMERKESRGLHYTLDYPDQIENPQPTILHP

Sequences:

>Translated_533_residues
MQSSSEHISDVLIIGSGAAGLSLALRLAPHCKVTVLSKGPLNEGATFYAQGGIAAVFDETDSISSHVDDTLIAGAGLCDK
EAVEFIASNARSCVQWLIDQGVLFDTETSANGEERYHLTREGGHSHRRILHTADATGKEVETTLVGKASNHPNISVKERC
NAVDLITSNKIGLAGTKRVVGAYVWNRELEKVETFRAKAVVLATGGAAKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
NQFHPTCLFHPQARNFLLTEALRGEGAYLKRPDGSRFMLDFDPRGELAPRDIVARAIDHEMKRLGADCMYLDISHKPTDF
VMQHFPMIYEKLLSLGIDLTKEAIPIVPAAHYTCGGVMVDQHGRTDLDGLYAIGEVSYTGLHGANRMASNSLLECLVYGW
SAAEDILTRLPTAKLAKHLPDWDESRVDNSDERVVIQHNWHELRLFMWDYVGIVRTTKRLERALRRINTLQQEIDEYYAN
FRISNNLLELRNLVQVAELIVRCAMERKESRGLHYTLDYPDQIENPQPTILHP
>Mature_533_residues
MQSSSEHISDVLIIGSGAAGLSLALRLAPHCKVTVLSKGPLNEGATFYAQGGIAAVFDETDSISSHVDDTLIAGAGLCDK
EAVEFIASNARSCVQWLIDQGVLFDTETSANGEERYHLTREGGHSHRRILHTADATGKEVETTLVGKASNHPNISVKERC
NAVDLITSNKIGLAGTKRVVGAYVWNRELEKVETFRAKAVVLATGGAAKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
NQFHPTCLFHPQARNFLLTEALRGEGAYLKRPDGSRFMLDFDPRGELAPRDIVARAIDHEMKRLGADCMYLDISHKPTDF
VMQHFPMIYEKLLSLGIDLTKEAIPIVPAAHYTCGGVMVDQHGRTDLDGLYAIGEVSYTGLHGANRMASNSLLECLVYGW
SAAEDILTRLPTAKLAKHLPDWDESRVDNSDERVVIQHNWHELRLFMWDYVGIVRTTKRLERALRRINTLQQEIDEYYAN
FRISNNLLELRNLVQVAELIVRCAMERKESRGLHYTLDYPDQIENPQPTILHP

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily

Homologues:

Organism=Homo sapiens, GI156416003, Length=516, Percent_Identity=31.2015503875969, Blast_Score=189, Evalue=7e-48,
Organism=Escherichia coli, GI1788928, Length=533, Percent_Identity=83.8649155722326, Blast_Score=928, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=524, Percent_Identity=32.4427480916031, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1786942, Length=532, Percent_Identity=30.8270676691729, Blast_Score=179, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17550100, Length=544, Percent_Identity=31.4338235294118, Blast_Score=206, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17505833, Length=539, Percent_Identity=30.6122448979592, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI71986328, Length=467, Percent_Identity=27.8372591006424, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322701, Length=528, Percent_Identity=32.3863636363636, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6322416, Length=530, Percent_Identity=31.1320754716981, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6320788, Length=485, Percent_Identity=27.0103092783505, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17137288, Length=535, Percent_Identity=31.214953271028, Blast_Score=189, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24655642, Length=535, Percent_Identity=31.214953271028, Blast_Score=189, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24655647, Length=535, Percent_Identity=31.214953271028, Blast_Score=189, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24663005, Length=532, Percent_Identity=29.1353383458647, Blast_Score=179, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NADB_YERPE (Q8ZD80)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AF0330
- RefSeq:   NP_668612.1
- RefSeq:   NP_993835.1
- RefSeq:   YP_002347657.1
- ProteinModelPortal:   Q8ZD80
- SMR:   Q8ZD80
- GeneID:   1146236
- GeneID:   1175540
- GeneID:   2764388
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO2710
- KEGG:   ypk:y1289
- KEGG:   ypm:YP_2514
- HOGENOM:   HBG293998
- OMA:   GAYIWNR
- ProtClustDB:   PRK09077
- BioCyc:   YPES187410:Y1289-MONOMER
- BioCyc:   YPES214092:YPO2710-MONOMER
- BRENDA:   1.4.3.16
- GO:   GO:0005737
- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288
- Gene3D:   G3DSA:1.20.58.100
- PRINTS:   PR00368
- TIGRFAMs:   TIGR00551

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C; SSF46977 Succ_DH_flav_C

EC number: =1.4.3.16

Molecular weight: Translated: 59295; Mature: 59295

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: ACT_SITE 244-244 ACT_SITE 263-263

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSSSEHISDVLIIGSGAAGLSLALRLAPHCKVTVLSKGPLNEGATFYAQGGIAAVFDET
CCCCHHCCCEEEEEECCCHHHHHHEEECCCCEEEEEECCCCCCCCEEEECCCEEEEECCC
DSISSHVDDTLIAGAGLCDKEAVEFIASNARSCVQWLIDQGVLFDTETSANGEERYHLTR
CHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEEC
EGGHSHRRILHTADATGKEVETTLVGKASNHPNISVKERCNAVDLITSNKIGLAGTKRVV
CCCCCCCEEEEECCCCCCEEEEEEEECCCCCCCEEHHHHCCEEEEEECCCCCCCCHHHHH
GAYVWNRELEKVETFRAKAVVLATGGAAKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
HHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCEEEEEEC
NQFHPTCLFHPQARNFLLTEALRGEGAYLKRPDGSRFMLDFDPRGELAPRDIVARAIDHE
CCCCCEEEECCCCCCHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHH
MKRLGADCMYLDISHKPTDFVMQHFPMIYEKLLSLGIDLTKEAIPIVPAAHYTCGGVMVD
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEE
QHGRTDLDGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAEDILTRLPTAKLAKHLP
CCCCCCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCC
DWDESRVDNSDERVVIQHNWHELRLFMWDYVGIVRTTKRLERALRRINTLQQEIDEYYAN
CCCHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FRISNNLLELRNLVQVAELIVRCAMERKESRGLHYTLDYPDQIENPQPTILHP
EEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHCCCCCCCCCCC
>Mature Secondary Structure
MQSSSEHISDVLIIGSGAAGLSLALRLAPHCKVTVLSKGPLNEGATFYAQGGIAAVFDET
CCCCHHCCCEEEEEECCCHHHHHHEEECCCCEEEEEECCCCCCCCEEEECCCEEEEECCC
DSISSHVDDTLIAGAGLCDKEAVEFIASNARSCVQWLIDQGVLFDTETSANGEERYHLTR
CHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEEC
EGGHSHRRILHTADATGKEVETTLVGKASNHPNISVKERCNAVDLITSNKIGLAGTKRVV
CCCCCCCEEEEECCCCCCEEEEEEEECCCCCCCEEHHHHCCEEEEEECCCCCCCCHHHHH
GAYVWNRELEKVETFRAKAVVLATGGAAKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
HHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCEEEEEEC
NQFHPTCLFHPQARNFLLTEALRGEGAYLKRPDGSRFMLDFDPRGELAPRDIVARAIDHE
CCCCCEEEECCCCCCHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHH
MKRLGADCMYLDISHKPTDFVMQHFPMIYEKLLSLGIDLTKEAIPIVPAAHYTCGGVMVD
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEE
QHGRTDLDGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAEDILTRLPTAKLAKHLP
CCCCCCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCC
DWDESRVDNSDERVVIQHNWHELRLFMWDYVGIVRTTKRLERALRRINTLQQEIDEYYAN
CCCHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FRISNNLLELRNLVQVAELIVRCAMERKESRGLHYTLDYPDQIENPQPTILHP
EEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430