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The map label for this gene is holB [H]

Identifier: 153947256

GI number: 153947256

Start: 1841094

End: 1842116

Strand: Direct

Name: holB [H]

Synonym: YpsIP31758_1584

Alternate gene names: 153947256

Gene position: 1841094-1842116 (Clockwise)

Preceding gene: 153946978

Following gene: 153947622

Centisome position: 38.98

GC content: 52.69

Gene sequence:

>1023_bases
ATGAATTGGTATCCGTGGCTTAACGGCCCTTACCGACAACTGGTTGGGCAGCACAGTACCGGGCGCGGGCACCATGCATT
ACTGCTGCATTCATTACCCGGTAATGGCGAAGACGCATTAATTTATGCGCTGAGTCGTTGGCTAATGTGTCAGCAGCGGC
AAGGTGAGAAGAGTTGTGGTGAATGCCATAGCTGCCGCTTGATGTTGGCAGGCAACCACCCCGATTGGTATGTACTGACA
CCGGAAAAAGGCAAAAGCAGTATCGGGGTTGAGTTGGTTCGCCAATTGATTGATAAACTGTATTCCCATGCTCAGCAAGG
CGGTGCAAAGGTGGTGTGGTTGCCACATGCCGAAGTGTTGACCGATGCCGCGGCTAATGCCCTGCTAAAAACGCTGGAAG
AGCCTCCAGAGAAGACCTATTTTCTGTTGGATTGCCACCAACCCGCCAGCTTGCTGGCTACACTGCGTAGCCGCTGTTTT
TATTGGTATCTGGCTTGCCCGGATACGGCTATTTGTCTGCAATGGCTCAATCTGCAATGGCGTAAGCGGCAGATCCCTGT
GGAGCCCGTTGCGATGTTGGCGGCCCTGAAATTGAGTGAAGGCGCACCGCTGGCGGCTGAGCGGTTGCTCCAACCAGAGC
GCTGGAGTATCCGCAGCGCTTTGTGTAGCGGGCTAAGCGAGGCGCTGAACCGCTCAGACTTGCTGTCGTTGCTACCACAA
CTTAACCATGATGATGCTGCTGAACGTCTGCAATGGCTCTCTTCACTGCTCTTGGATGCGCTGAAGTGGCAGCAAGGGGC
AGGGGAGTTTGCGGTTAATCAGGATCAATTGCCATTAGTGCAACAGTTGGCACACATCGCGGCCACGCCAGTATTACTTC
AGTTAGCTAAGCAACTGGCTCATTGCCGCCATCAATTATTATCTGTGGTAGGTGTTAATCGTGAATTGTTACTCACTGAG
CAACTGCTTAACTGGGAAACAGCACTGTCCACGGGTACTTACTCCACCTTACCTTCGTTGTAA

Upstream 100 bases:

>100_bases
CCAGTATTAAAACGATTGATGCCTCTCAGCCCATTGAACAAGTTAGTGCGTCGATAAGCCAGGCGCTCGCTCAGTGGCTG
ACCAATCAGGAACTCGTGTA

Downstream 100 bases:

>100_bases
AAGAGTTTAATATTATGTTGTTAGTTGATTCTCATTGCCACCTTGATAGCCTTGATTACCAGACATTGCACAGCAGTGTC
GATGACGTATTAGCCAAAGC

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MNWYPWLNGPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCGECHSCRLMLAGNHPDWYVLT
PEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVLTDAAANALLKTLEEPPEKTYFLLDCHQPASLLATLRSRCF
YWYLACPDTAICLQWLNLQWRKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLSEALNRSDLLSLLPQ
LNHDDAAERLQWLSSLLLDALKWQQGAGEFAVNQDQLPLVQQLAHIAATPVLLQLAKQLAHCRHQLLSVVGVNRELLLTE
QLLNWETALSTGTYSTLPSL

Sequences:

>Translated_340_residues
MNWYPWLNGPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCGECHSCRLMLAGNHPDWYVLT
PEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVLTDAAANALLKTLEEPPEKTYFLLDCHQPASLLATLRSRCF
YWYLACPDTAICLQWLNLQWRKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLSEALNRSDLLSLLPQ
LNHDDAAERLQWLSSLLLDALKWQQGAGEFAVNQDQLPLVQQLAHIAATPVLLQLAKQLAHCRHQLLSVVGVNRELLLTE
QLLNWETALSTGTYSTLPSL
>Mature_340_residues
MNWYPWLNGPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCGECHSCRLMLAGNHPDWYVLT
PEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVLTDAAANALLKTLEEPPEKTYFLLDCHQPASLLATLRSRCF
YWYLACPDTAICLQWLNLQWRKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLSEALNRSDLLSLLPQ
LNHDDAAERLQWLSSLLLDALKWQQGAGEFAVNQDQLPLVQQLAHIAATPVLLQLAKQLAHCRHQLLSVVGVNRELLLTE
QLLNWETALSTGTYSTLPSL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=340, Percent_Identity=50.8823529411765, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1786676, Length=207, Percent_Identity=30.4347826086957, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 38058; Mature: 38058

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWYPWLNGPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCG
CCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
ECHSCRLMLAGNHPDWYVLTPEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVL
CHHHCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHH
TDAAANALLKTLEEPPEKTYFLLDCHQPASLLATLRSRCFYWYLACPDTAICLQWLNLQW
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHCCHH
RKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLSEALNRSDLLSLLPQ
HHCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHC
LNHDDAAERLQWLSSLLLDALKWQQGAGEFAVNQDQLPLVQQLAHIAATPVLLQLAKQLA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HCRHQLLSVVGVNRELLLTEQLLNWETALSTGTYSTLPSL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNWYPWLNGPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCG
CCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
ECHSCRLMLAGNHPDWYVLTPEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVL
CHHHCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHH
TDAAANALLKTLEEPPEKTYFLLDCHQPASLLATLRSRCFYWYLACPDTAICLQWLNLQW
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHCCHH
RKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLSEALNRSDLLSLLPQ
HHCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHC
LNHDDAAERLQWLSSLLLDALKWQQGAGEFAVNQDQLPLVQQLAHIAATPVLLQLAKQLA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HCRHQLLSVVGVNRELLLTEQLLNWETALSTGTYSTLPSL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430; 10491164 [H]