Click here to switch to the map view.

The map label for this gene is rluE [H]

Identifier: 153947226

GI number: 153947226

Start: 1873164

End: 1873790

Strand: Reverse

Name: rluE [H]

Synonym: YpsIP31758_1614

Alternate gene names: 153947226

Gene position: 1873790-1873164 (Counterclockwise)

Preceding gene: 153947306

Following gene: 153950458

Centisome position: 39.67

GC content: 48.48

Gene sequence:

>627_bases
ATGAATAAATTCTCTGTTAAAAATCACAAAGTTAACCGATTCAGCACTAAGGCACAAGCCAGGCAGTCCCCCCCCGCTGC
GCCACGGCGGGTATTGTTATTCAATAAACCTTTTGATGTATTACCGCAATTCACTGATGAGTCTGGACGCACCACTTTGC
AGGGTTTCATCCCTTTTAAGGACGTTTATGCTGCTGGCCGGTTAGATCGCGATAGCGAGGGCTTATTGGTATTAACCAAT
GATGGCAAGTTGCAAGCCAGGCTCACGCAACCGGCGCAAAAAACAGGTAAAGTCTATTTTGTACAAGTAGAAGGGGTCCC
TGACAACGATGCGCTCACACAGCTAAAACGAGGCGTAACGTTGAAAGATGGCCTGACGTTACCCGCAGGCGCGGAGTTAG
TGGCAGAGCCTGAATGGCTATGGCCGCGCCATCCCCCTATCAGAGAGCGTAAAACCATCCCAACCAGTTGGTTGAAAATA
ACACTTTATGAAGGCCGCAACCGGCAAGTACGTAGAATAACCGCACATGTTGGCTTCCCTACCCTGCGCCTGATTCGTTT
TAGTATAGGAAATTTAAGCCTTGGCAACTTACAGCCCGGCGAATGGAAGGAGATAACTGATGTTTAA

Upstream 100 bases:

>100_bases
GCTTTGTAAGATTCGTGTCAATACTACTTGAATATTCGTTCCGCGCCAATCCGAAACGGATTGGGGTATAATGCGCTAAT
CATTCGAAACCGCAATAATC

Downstream 100 bases:

>100_bases
ACCGCATGTTACTGTCGCCTGTGTCGTTCATACTCAGGGGAAATTTCTGATCGTTGAAGAAACCATCAACAATGAAAAAC
GCTGGAACCAGCCAGCCGGT

Product: ribosomal large subunit pseudouridine synthase E

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MNKFSVKNHKVNRFSTKAQARQSPPAAPRRVLLFNKPFDVLPQFTDESGRTTLQGFIPFKDVYAAGRLDRDSEGLLVLTN
DGKLQARLTQPAQKTGKVYFVQVEGVPDNDALTQLKRGVTLKDGLTLPAGAELVAEPEWLWPRHPPIRERKTIPTSWLKI
TLYEGRNRQVRRITAHVGFPTLRLIRFSIGNLSLGNLQPGEWKEITDV

Sequences:

>Translated_208_residues
MNKFSVKNHKVNRFSTKAQARQSPPAAPRRVLLFNKPFDVLPQFTDESGRTTLQGFIPFKDVYAAGRLDRDSEGLLVLTN
DGKLQARLTQPAQKTGKVYFVQVEGVPDNDALTQLKRGVTLKDGLTLPAGAELVAEPEWLWPRHPPIRERKTIPTSWLKI
TLYEGRNRQVRRITAHVGFPTLRLIRFSIGNLSLGNLQPGEWKEITDV
>Mature_208_residues
MNKFSVKNHKVNRFSTKAQARQSPPAAPRRVLLFNKPFDVLPQFTDESGRTTLQGFIPFKDVYAAGRLDRDSEGLLVLTN
DGKLQARLTQPAQKTGKVYFVQVEGVPDNDALTQLKRGVTLKDGLTLPAGAELVAEPEWLWPRHPPIRERKTIPTSWLKI
TLYEGRNRQVRRITAHVGFPTLRLIRFSIGNLSLGNLQPGEWKEITDV

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=207, Percent_Identity=72.463768115942, Blast_Score=319, Evalue=8e-89,
Organism=Escherichia coli, GI1787524, Length=187, Percent_Identity=36.3636363636364, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788510, Length=184, Percent_Identity=32.6086956521739, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI1790453, Length=186, Percent_Identity=31.7204301075269, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 23507; Mature: 23507

Theoretical pI: Translated: 10.92; Mature: 10.92

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFSVKNHKVNRFSTKAQARQSPPAAPRRVLLFNKPFDVLPQFTDESGRTTLQGFIPFK
CCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHCCCCCCCCCCEEEEECCCHH
DVYAAGRLDRDSEGLLVLTNDGKLQARLTQPAQKTGKVYFVQVEGVPDNDALTQLKRGVT
HHHHHCCCCCCCCCEEEEECCCEEEEEECCCHHHCCCEEEEEECCCCCCHHHHHHHCCCE
LKDGLTLPAGAELVAEPEWLWPRHPPIRERKTIPTSWLKITLYEGRNRQVRRITAHVGFP
ECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCC
TLRLIRFSIGNLSLGNLQPGEWKEITDV
CEEEEEEECCCEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MNKFSVKNHKVNRFSTKAQARQSPPAAPRRVLLFNKPFDVLPQFTDESGRTTLQGFIPFK
CCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHCCCCCCCCCCEEEEECCCHH
DVYAAGRLDRDSEGLLVLTNDGKLQARLTQPAQKTGKVYFVQVEGVPDNDALTQLKRGVT
HHHHHCCCCCCCCCEEEEECCCEEEEEECCCHHHCCCEEEEEECCCCCCHHHHHHHCCCE
LKDGLTLPAGAELVAEPEWLWPRHPPIRERKTIPTSWLKITLYEGRNRQVRRITAHVGFP
ECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCC
TLRLIRFSIGNLSLGNLQPGEWKEITDV
CEEEEEEECCCEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]