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The map label for this gene is insB [H]

Identifier: 153947068

GI number: 153947068

Start: 907775

End: 908143

Strand: Direct

Name: insB [H]

Synonym: YpsIP31758_0741

Alternate gene names: 153947068

Gene position: 907775-908143 (Clockwise)

Preceding gene: 153947493

Following gene: 153947502

Centisome position: 19.22

GC content: 46.34

Gene sequence:

>369_bases
ATGTGGTCATTTGTGGGCAACAAAAAGCAGCAACGTTGGCTTTGGTATGCCTGGGAGCCTCGCCTCAAACGTATTATTGC
TCATGTTTTTGGTCGTCGGAGCAAAAAGACATTTCGCCAATTATTGGGGTTGCTGTCAGGTTTCAATATCGTCTTCTGGT
GTACCGACAACTTCAGTGCTTATGAGATGTTGCCGGATGAAAAACACATCAGGAGCAAGCTTTACACGCAGCGGATTGAG
CGTGAAAACCTGAATATCCGTAATCGGTTGAAACGACTGAACCGCAAGACTCTCGGAGACTCAAAGTCGGCAGAAATGCA
TAACAGGATCATCGGTACGTTCATTGAGCGTGAGCATTACCTAGTGTGA

Upstream 100 bases:

>100_bases
TCAATGCGGTCATGCGCACTTTAAAAAACTCTCGCCGCAGCGTGTAACCACGCTCCCACTGGATAACCTGCAAATTCAGC
TTATCTGTGAAGTCGATGAA

Downstream 100 bases:

>100_bases
TGCTAATAACCAGATTGAATACATGACCGAATTTTTATTTTTATGGTGTTTGTCAGTGGGTGCGTGTCAACTATAACTTT
TGAACCGAGTGAATTGGCGG

Product: IS1 family transposase orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 122; Mature: 122

Protein sequence:

>122_residues
MWSFVGNKKQQRWLWYAWEPRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNFSAYEMLPDEKHIRSKLYTQRIE
RENLNIRNRLKRLNRKTLGDSKSAEMHNRIIGTFIEREHYLV

Sequences:

>Translated_122_residues
MWSFVGNKKQQRWLWYAWEPRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNFSAYEMLPDEKHIRSKLYTQRIE
RENLNIRNRLKRLNRKTLGDSKSAEMHNRIIGTFIEREHYLV
>Mature_122_residues
MWSFVGNKKQQRWLWYAWEPRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNFSAYEMLPDEKHIRSKLYTQRIE
RENLNIRNRLKRLNRKTLGDSKSAEMHNRIIGTFIEREHYLV

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=120, Percent_Identity=49.1666666666667, Blast_Score=123, Evalue=4e-30,
Organism=Escherichia coli, GI1788203, Length=120, Percent_Identity=49.1666666666667, Blast_Score=123, Evalue=4e-30,
Organism=Escherichia coli, GI1786203, Length=120, Percent_Identity=49.1666666666667, Blast_Score=123, Evalue=4e-30,
Organism=Escherichia coli, GI1787223, Length=120, Percent_Identity=49.1666666666667, Blast_Score=121, Evalue=2e-29,
Organism=Escherichia coli, GI1786470, Length=120, Percent_Identity=49.1666666666667, Blast_Score=120, Evalue=2e-29,
Organism=Escherichia coli, GI1786459, Length=120, Percent_Identity=49.1666666666667, Blast_Score=120, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 14928; Mature: 14928

Theoretical pI: Translated: 11.26; Mature: 11.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWSFVGNKKQQRWLWYAWEPRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNFSA
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCH
YEMLPDEKHIRSKLYTQRIERENLNIRNRLKRLNRKTLGDSKSAEMHNRIIGTFIEREHY
HHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
LV
CC
>Mature Secondary Structure
MWSFVGNKKQQRWLWYAWEPRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNFSA
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCH
YEMLPDEKHIRSKLYTQRIERENLNIRNRLKRLNRKTLGDSKSAEMHNRIIGTFIEREHY
HHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
LV
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]