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The map label for this gene is rlmF

Identifier: 153946932

GI number: 153946932

Start: 1731845

End: 1732855

Strand: Direct

Name: rlmF

Synonym: YpsIP31758_1491

Alternate gene names: 153946932

Gene position: 1731845-1732855 (Clockwise)

Preceding gene: 153948072

Following gene: 153950307

Centisome position: 36.67

GC content: 49.26

Gene sequence:

>1011_bases
ATGCTATCTTACGCGCCAGAAAATGCCTATCAGAGAGCCAGTACGATGGAAAATAAAAAAGTATTCCCCAAAGAAAAAAG
CGGATTGCATCCCCGAAATCGTCATCGCTCACGCTATGATTTTGATGCGCTTTCAGTGAGTTGCCCTGAGCTAATCCCTT
TTCTGGCCCCGACCGCCTATGGTGATATTTCTGTCGATTTTGCTGATCCATTAGCAGTAAAAATGTTGAATAAGGCATTA
TTAAAACATTTCTATGGCATTGAATATTGGGATATTCCAGCCGATTCTTTATGTCCTCCCATTCCTGGGCGTGCCGATTA
CGTGCACCATCTGGCCGATCTATTAGCCAGTTGCAATGGCGAGGTGATCCCTAAGGGCAAGAATATTGCGTTGCTGGATA
TTGGCGTCGGCGCTAACTGTATTTATCCAATTATTGGTCAGCGGGAGTACGGCTGGCGCTTTACCGGTACGGATATTGAC
TCACACGCGCTCAGTGCGGCAAAAATGGTGGTGTCGATGAACCCGACCCTGAAAAATACCTTGCGCCTGAAGCAGCAAAA
AGATCCACACGCTATTTTTGAAGGGGTGTGGGCGGTGAATGAGCGTTACGATGCGACGCTGTGTAATCCGCCATTCCATG
GTTCAGCAGAAGAAGCGGCGGCCACCACTCGCCGTAAATTGCACAAACTGGGGAAAAATGAAGTCGCTGCAAAGCCAGTA
CAGAACTTCGGTGGTAAAAACAGTGAGTTATGGTGTGAAGGTGGAGAGGAAGGGTTTGTCAGCCGCATGGTGGCAGAGAG
CGTAGCGAAAGCGCAGAACTGCTTCTGGTTTACCTCATTGATTTCCAAGAAGACCACGTTACCGGCGATTTATCATGCGT
TAAGATATGTGAAGGCAGTTGAGGTGCGCACTATTGAGATGGCGCAAGGGCAAAAAGTCAGTCGGTTTGTGGCCTGGACG
TTCCTGACGCCCGAGCAGCAAGCCGCGTGGGTTGCAGAGCGCTGGGCATAA

Upstream 100 bases:

>100_bases
CGATGGGCCAGCAGCGGATCATCATGTTGTTTGACATCGGCCACACAAAGGGGCTGGTGGTGTAGAACCTATTCACCGTA
CACCCGTTTAGCCGTAATTT

Downstream 100 bases:

>100_bases
CGTTTCCACTATATAAGCCAAATAAGCCAAATAAGCCAAATAAGCCAAATAAGCCAGTGACCACGACAAGTCAGTGATCA
CGATCAACCCGTTACCGTGG

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MLSYAPENAYQRASTMENKKVFPKEKSGLHPRNRHRSRYDFDALSVSCPELIPFLAPTAYGDISVDFADPLAVKMLNKAL
LKHFYGIEYWDIPADSLCPPIPGRADYVHHLADLLASCNGEVIPKGKNIALLDIGVGANCIYPIIGQREYGWRFTGTDID
SHALSAAKMVVSMNPTLKNTLRLKQQKDPHAIFEGVWAVNERYDATLCNPPFHGSAEEAAATTRRKLHKLGKNEVAAKPV
QNFGGKNSELWCEGGEEGFVSRMVAESVAKAQNCFWFTSLISKKTTLPAIYHALRYVKAVEVRTIEMAQGQKVSRFVAWT
FLTPEQQAAWVAERWA

Sequences:

>Translated_336_residues
MLSYAPENAYQRASTMENKKVFPKEKSGLHPRNRHRSRYDFDALSVSCPELIPFLAPTAYGDISVDFADPLAVKMLNKAL
LKHFYGIEYWDIPADSLCPPIPGRADYVHHLADLLASCNGEVIPKGKNIALLDIGVGANCIYPIIGQREYGWRFTGTDID
SHALSAAKMVVSMNPTLKNTLRLKQQKDPHAIFEGVWAVNERYDATLCNPPFHGSAEEAAATTRRKLHKLGKNEVAAKPV
QNFGGKNSELWCEGGEEGFVSRMVAESVAKAQNCFWFTSLISKKTTLPAIYHALRYVKAVEVRTIEMAQGQKVSRFVAWT
FLTPEQQAAWVAERWA
>Mature_336_residues
MLSYAPENAYQRASTMENKKVFPKEKSGLHPRNRHRSRYDFDALSVSCPELIPFLAPTAYGDISVDFADPLAVKMLNKAL
LKHFYGIEYWDIPADSLCPPIPGRADYVHHLADLLASCNGEVIPKGKNIALLDIGVGANCIYPIIGQREYGWRFTGTDID
SHALSAAKMVVSMNPTLKNTLRLKQQKDPHAIFEGVWAVNERYDATLCNPPFHGSAEEAAATTRRKLHKLGKNEVAAKPV
QNFGGKNSELWCEGGEEGFVSRMVAESVAKAQNCFWFTSLISKKTTLPAIYHALRYVKAVEVRTIEMAQGQKVSRFVAWT
FLTPEQQAAWVAERWA

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family

Homologues:

Organism=Homo sapiens, GI122114654, Length=295, Percent_Identity=29.8305084745763, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI87081786, Length=307, Percent_Identity=57.6547231270358, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32564931, Length=318, Percent_Identity=27.9874213836478, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32564933, Length=318, Percent_Identity=25.4716981132075, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI281363415, Length=308, Percent_Identity=28.2467532467532, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19922302, Length=308, Percent_Identity=28.2467532467532, Blast_Score=125, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMF_YERP3 (A7FGU1)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001400469.1
- ProteinModelPortal:   A7FGU1
- SMR:   A7FGU1
- STRING:   A7FGU1
- GeneID:   5387444
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_1491
- eggNOG:   COG3129
- HOGENOM:   HBG747880
- OMA:   AELWCEG
- ProtClustDB:   PRK11727
- BioCyc:   YPSE349747:YPSIP31758_1491-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01848
- InterPro:   IPR016909
- InterPro:   IPR010286
- PANTHER:   PTHR13393
- PIRSF:   PIRSF029038

Pfam domain/function: PF05971 Methyltransf_10

EC number: =2.1.1.181

Molecular weight: Translated: 37530; Mature: 37530

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSYAPENAYQRASTMENKKVFPKEKSGLHPRNRHRSRYDFDALSVSCPELIPFLAPTAY
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCHHHCCCCHHHHHHHCCCCC
GDISVDFADPLAVKMLNKALLKHFYGIEYWDIPADSLCPPIPGRADYVHHLADLLASCNG
CCEEECCCCHHHHHHHHHHHHHHHCCCCEECCCHHHCCCCCCCHHHHHHHHHHHHHHCCC
EVIPKGKNIALLDIGVGANCIYPIIGQREYGWRFTGTDIDSHALSAAKMVVSMNPTLKNT
CEEECCCCEEEEEECCCCCCHHHCCCCCCCCCEEECCCCCHHHHHHHHHHEECCCCHHHH
LRLKQQKDPHAIFEGVWAVNERYDATLCNPPFHGSAEEAAATTRRKLHKLGKNEVAAKPV
HHHHCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCHH
QNFGGKNSELWCEGGEEGFVSRMVAESVAKAQNCFWFTSLISKKTTLPAIYHALRYVKAV
HHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
EVRTIEMAQGQKVSRFVAWTFLTPEQQAAWVAERWA
HHEEEEHHCCCCHHHEEEEEEECCHHHHHHHHHHCC
>Mature Secondary Structure
MLSYAPENAYQRASTMENKKVFPKEKSGLHPRNRHRSRYDFDALSVSCPELIPFLAPTAY
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCHHHCCCCHHHHHHHCCCCC
GDISVDFADPLAVKMLNKALLKHFYGIEYWDIPADSLCPPIPGRADYVHHLADLLASCNG
CCEEECCCCHHHHHHHHHHHHHHHCCCCEECCCHHHCCCCCCCHHHHHHHHHHHHHHCCC
EVIPKGKNIALLDIGVGANCIYPIIGQREYGWRFTGTDIDSHALSAAKMVVSMNPTLKNT
CEEECCCCEEEEEECCCCCCHHHCCCCCCCCCEEECCCCCHHHHHHHHHHEECCCCHHHH
LRLKQQKDPHAIFEGVWAVNERYDATLCNPPFHGSAEEAAATTRRKLHKLGKNEVAAKPV
HHHHCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCHH
QNFGGKNSELWCEGGEEGFVSRMVAESVAKAQNCFWFTSLISKKTTLPAIYHALRYVKAV
HHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
EVRTIEMAQGQKVSRFVAWTFLTPEQQAAWVAERWA
HHEEEEHHCCCCHHHEEEEEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA