Click here to switch to the map view.

The map label for this gene is phnF [H]

Identifier: 153946914

GI number: 153946914

Start: 4020966

End: 4021691

Strand: Direct

Name: phnF [H]

Synonym: YpsIP31758_3559

Alternate gene names: 153946914

Gene position: 4020966-4021691 (Clockwise)

Preceding gene: 153950171

Following gene: 153947236

Centisome position: 85.13

GC content: 56.75

Gene sequence:

>726_bases
ATGAACTTATCTAGACCACCGACCAGCTATCCCACCCGTTATCAACATATTGCGGCCCAGTTAGAGCAGGAGTTGCGCGG
CCATTACCGTTGTGGCGATTATCTGCCCTCCGAACAACAGCTCGCCGACCGCTATCAGGTTAACCGCCATACCCTGCGCC
GGGCAGTGGATGAATTGGTCGAGCGCGGCTGGCTACAACGCCGCCAGGGCATCGGCATTCTGGTGCTCATGCGCCCTTAT
GACTACCCGCTCCATGCCAATGCGCGTTTCAGCCAGAACCTACTCGATCAAGGCAGTGACCCCACCAGTGAGCGCTTACT
TGCCGTTTTACGCCCTGCGGCCGAGCCTGTTGCAAAAGCACTCTCGCTGGCAGAGGGGGAATCGGTCGTTCATTTGCGCA
CCCTGAGGCGGGTTAATGGTGTGCCGGTCTGCATTATCGATCACTACCTGCCCGATCTCAGTTGGTGGCCAGTTCTACAA
CAGTTTCACTCGGGTTCACTGCATCAGTTCATCACACAACATTTACAGCAGCCTCTGAAACGTAGCCAAACGCGCATCAG
CGCCCGGCGTGCTCAAGCAAAAGAGAGCGCCTTGCTGGAGATTGCCACCCATGCGCCGCTCCTGTGTGTGCGGACGTTGA
ATACCCGTGTTGATAACCAACAGGTGGCGGAATATTCCCTCAGCCTGACTCGCGCCGACATGATTGAACTGACCATGGAG
CACTGA

Upstream 100 bases:

>100_bases
TGACAATCCGCCACCTGCCCGCCTGCTTTTTCTTTTCATGTCGTTGTCTTGGACAAAAAGCGGTAGCCCGTGGTGATGAT
GCTGAGAGGGTATATTTGCC

Downstream 100 bases:

>100_bases
ATGGAAAGCACGCTGATGACCCGGCAAAACTGGATGTCGATCTTGGCCCATAGCCAGCCCACAGAACTTCTCACCCACTG
GCAGCCCTTAAATCTATCAC

Product: phosphonate metabolism transcriptional regulator PhnF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MNLSRPPTSYPTRYQHIAAQLEQELRGHYRCGDYLPSEQQLADRYQVNRHTLRRAVDELVERGWLQRRQGIGILVLMRPY
DYPLHANARFSQNLLDQGSDPTSERLLAVLRPAAEPVAKALSLAEGESVVHLRTLRRVNGVPVCIIDHYLPDLSWWPVLQ
QFHSGSLHQFITQHLQQPLKRSQTRISARRAQAKESALLEIATHAPLLCVRTLNTRVDNQQVAEYSLSLTRADMIELTME
H

Sequences:

>Translated_241_residues
MNLSRPPTSYPTRYQHIAAQLEQELRGHYRCGDYLPSEQQLADRYQVNRHTLRRAVDELVERGWLQRRQGIGILVLMRPY
DYPLHANARFSQNLLDQGSDPTSERLLAVLRPAAEPVAKALSLAEGESVVHLRTLRRVNGVPVCIIDHYLPDLSWWPVLQ
QFHSGSLHQFITQHLQQPLKRSQTRISARRAQAKESALLEIATHAPLLCVRTLNTRVDNQQVAEYSLSLTRADMIELTME
H
>Mature_241_residues
MNLSRPPTSYPTRYQHIAAQLEQELRGHYRCGDYLPSEQQLADRYQVNRHTLRRAVDELVERGWLQRRQGIGILVLMRPY
DYPLHANARFSQNLLDQGSDPTSERLLAVLRPAAEPVAKALSLAEGESVVHLRTLRRVNGVPVCIIDHYLPDLSWWPVLQ
QFHSGSLHQFITQHLQQPLKRSQTRISARRAQAKESALLEIATHAPLLCVRTLNTRVDNQQVAEYSLSLTRADMIELTME
H

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known. By similarity could be a transcriptional regulator [H]

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790540, Length=241, Percent_Identity=72.1991701244813, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1786950, Length=232, Percent_Identity=28.448275862069, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790118, Length=208, Percent_Identity=29.8076923076923, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082252, Length=242, Percent_Identity=25.2066115702479, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788418, Length=216, Percent_Identity=26.8518518518519, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012702
- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 27693; Mature: 27693

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSRPPTSYPTRYQHIAAQLEQELRGHYRCGDYLPSEQQLADRYQVNRHTLRRAVDELV
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ERGWLQRRQGIGILVLMRPYDYPLHANARFSQNLLDQGSDPTSERLLAVLRPAAEPVAKA
HHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LSLAEGESVVHLRTLRRVNGVPVCIIDHYLPDLSWWPVLQQFHSGSLHQFITQHLQQPLK
HHHHCCCCHHHHHHHHHHCCCCEEEEHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
RSQTRISARRAQAKESALLEIATHAPLLCVRTLNTRVDNQQVAEYSLSLTRADMIELTME
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
H
C
>Mature Secondary Structure
MNLSRPPTSYPTRYQHIAAQLEQELRGHYRCGDYLPSEQQLADRYQVNRHTLRRAVDELV
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ERGWLQRRQGIGILVLMRPYDYPLHANARFSQNLLDQGSDPTSERLLAVLRPAAEPVAKA
HHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LSLAEGESVVHLRTLRRVNGVPVCIIDHYLPDLSWWPVLQQFHSGSLHQFITQHLQQPLK
HHHHCCCCHHHHHHHHHHCCCCEEEEHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
RSQTRISARRAQAKESALLEIATHAPLLCVRTLNTRVDNQQVAEYSLSLTRADMIELTME
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
H
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]