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The map label for this gene is xylF [H]

Identifier: 153946825

GI number: 153946825

Start: 4625542

End: 4626537

Strand: Reverse

Name: xylF [H]

Synonym: YpsIP31758_4101

Alternate gene names: 153946825

Gene position: 4626537-4625542 (Counterclockwise)

Preceding gene: 153950926

Following gene: 153948579

Centisome position: 97.95

GC content: 41.87

Gene sequence:

>996_bases
ATGAAATTTAAGAACATTTTACTCTCCGCATGCGCTGCACTGGTAATGTTTAGTCAGCCTGGATTTAGCAAAGAAATTAA
AATCGGTATGGCGATTGATGACTTACGTCTTGAGCGCTGGCAAAAAGACCGCGATATTTTTGTTAACAAGGCTGAATCCC
TCGGTGCTAAAGTTTTTGTTCAATCGGCCAATGGCAATGAAGAAACACAAATGGCGCAGATTGAAAATATGATTAACCGT
GGTGTCGATGTCCTGGTTATTATTCCCTACAACGGGCAAGTATTAAGTAATGTGATCGCTGAAGCAAAACGGGAAGGCAT
AAAAGTGTTGGCTTATGATCGCATGATAAATAACGCTGACATTGATTTCTATATCTCCTTTGACAATGAAAAAGTAGGTG
AATTACAAGCTAAAAATTTGGTTGAACGGGTACCTCAGGGCAATTATTTCCTGATGGGCGGTTCACCAGTGGATAATAAT
GCTAAATTATTCCGTCAGGGGCAGATGACTGTTCTTAATCCATTGATAAAGGACGGTAAAATCAAGATTGTAGGTGACCA
ATGGGTTGATGCCTGGCTACCAGAAAATGCGTTAAAAATCATGGAAAATGCCTTAACGGCAAACAATAACAATATTGATG
CTGTGGTGGCCTCTAATGATGCAACCGCCGGTGGTGCCATTCAGGCTTTAGCCGCACAAGGGCTAGCCGGTAAAGTGGCT
ATTTCTGGTCAAGACGCAGATTTGGCCGCTATCAAGCGTATTGTTGCCGGTACGCAAACCATGACGGTATACAAGCCCAT
CAGTAAATTGGCCAATGATGCGGCTGAGATCGCCGTGACATTGGGTAATGGTGAGCAACCGAAAGCAAACAGCACGTTAA
ATAACGGTATGAAAGATGTTCCTGCTTATTTGTTAACACCTATTCAGGTCGATAAAAATAATATTGATAGCACCATCATT
GCTGACGGGTTCCACAAAAAAGCAGATATTTACTAA

Upstream 100 bases:

>100_bases
TCTGTAATTGCCGATGTGCTAATAAGCGTCAACAATCCTATTGCAGTTGATGTTTACCGTTTCTCTTACCCTGCTTGCAC
AATAAGAAAGGTACCAAAAG

Downstream 100 bases:

>100_bases
TTAACATATCGCTGATTGGTTTTATTATTTTAATAAATGGCCAGTGTTTGTAAGGCATTAATTTTGAATTAATAGACTCT
CAATCACCTGGCCGTTATTT

Product: D-xylose transporter subunit XylF

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKFKNILLSACAALVMFSQPGFSKEIKIGMAIDDLRLERWQKDRDIFVNKAESLGAKVFVQSANGNEETQMAQIENMINR
GVDVLVIIPYNGQVLSNVIAEAKREGIKVLAYDRMINNADIDFYISFDNEKVGELQAKNLVERVPQGNYFLMGGSPVDNN
AKLFRQGQMTVLNPLIKDGKIKIVGDQWVDAWLPENALKIMENALTANNNNIDAVVASNDATAGGAIQALAAQGLAGKVA
ISGQDADLAAIKRIVAGTQTMTVYKPISKLANDAAEIAVTLGNGEQPKANSTLNNGMKDVPAYLLTPIQVDKNNIDSTII
ADGFHKKADIY

Sequences:

>Translated_331_residues
MKFKNILLSACAALVMFSQPGFSKEIKIGMAIDDLRLERWQKDRDIFVNKAESLGAKVFVQSANGNEETQMAQIENMINR
GVDVLVIIPYNGQVLSNVIAEAKREGIKVLAYDRMINNADIDFYISFDNEKVGELQAKNLVERVPQGNYFLMGGSPVDNN
AKLFRQGQMTVLNPLIKDGKIKIVGDQWVDAWLPENALKIMENALTANNNNIDAVVASNDATAGGAIQALAAQGLAGKVA
ISGQDADLAAIKRIVAGTQTMTVYKPISKLANDAAEIAVTLGNGEQPKANSTLNNGMKDVPAYLLTPIQVDKNNIDSTII
ADGFHKKADIY
>Mature_331_residues
MKFKNILLSACAALVMFSQPGFSKEIKIGMAIDDLRLERWQKDRDIFVNKAESLGAKVFVQSANGNEETQMAQIENMINR
GVDVLVIIPYNGQVLSNVIAEAKREGIKVLAYDRMINNADIDFYISFDNEKVGELQAKNLVERVPQGNYFLMGGSPVDNN
AKLFRQGQMTVLNPLIKDGKIKIVGDQWVDAWLPENALKIMENALTANNNNIDAVVASNDATAGGAIQALAAQGLAGKVA
ISGQDADLAAIKRIVAGTQTMTVYKPISKLANDAAEIAVTLGNGEQPKANSTLNNGMKDVPAYLLTPIQVDKNNIDSTII
ADGFHKKADIY

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=330, Percent_Identity=82.1212121212121, Blast_Score=542, Evalue=1e-155,
Organism=Escherichia coli, GI1790526, Length=226, Percent_Identity=26.9911504424779, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35913; Mature: 35913

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKNILLSACAALVMFSQPGFSKEIKIGMAIDDLRLERWQKDRDIFVNKAESLGAKVFV
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHHHHHHCCCHHHEEECHHCCCEEEEE
QSANGNEETQMAQIENMINRGVDVLVIIPYNGQVLSNVIAEAKREGIKVLAYDRMINNAD
ECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCC
IDFYISFDNEKVGELQAKNLVERVPQGNYFLMGGSPVDNNAKLFRQGQMTVLNPLIKDGK
EEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHCCCCHHHHHHHHCCCE
IKIVGDQWVDAWLPENALKIMENALTANNNNIDAVVASNDATAGGAIQALAAQGLAGKVA
EEEEECCHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEE
ISGQDADLAAIKRIVAGTQTMTVYKPISKLANDAAEIAVTLGNGEQPKANSTLNNGMKDV
ECCCCCHHHHHHHHHHCCCEEEHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHCCHHHC
PAYLLTPIQVDKNNIDSTIIADGFHKKADIY
CHHHCCCEEECCCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MKFKNILLSACAALVMFSQPGFSKEIKIGMAIDDLRLERWQKDRDIFVNKAESLGAKVFV
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHHHHHHCCCHHHEEECHHCCCEEEEE
QSANGNEETQMAQIENMINRGVDVLVIIPYNGQVLSNVIAEAKREGIKVLAYDRMINNAD
ECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCC
IDFYISFDNEKVGELQAKNLVERVPQGNYFLMGGSPVDNNAKLFRQGQMTVLNPLIKDGK
EEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHCCCCHHHHHHHHCCCE
IKIVGDQWVDAWLPENALKIMENALTANNNNIDAVVASNDATAGGAIQALAAQGLAGKVA
EEEEECCHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEE
ISGQDADLAAIKRIVAGTQTMTVYKPISKLANDAAEIAVTLGNGEQPKANSTLNNGMKDV
ECCCCCHHHHHHHHHHCCCEEEHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHCCHHHC
PAYLLTPIQVDKNNIDSTIIADGFHKKADIY
CHHHCCCEEECCCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]