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The map label for this gene is kgtP [H]

Identifier: 153952597

GI number: 153952597

Start: 295491

End: 296765

Strand: Direct

Name: kgtP [H]

Synonym: JJD26997_0335

Alternate gene names: 153952597

Gene position: 295491-296765 (Clockwise)

Preceding gene: 153952101

Following gene: 153952264

Centisome position: 16.01

GC content: 37.49

Gene sequence:

>1275_bases
ATGCAAACAACCAAACTAAGCAAAAAGGCTCAAATTCACTCTGTTATCGCTGCAAGTAGTGGCAATCTTGTTGAATGGTT
TGACTTTTATATTTATGGTTTTGCGGCTGTGTATTTTGCTCATAATTTTTCTAATGCCACTTCAACACTTTTTCAGCAAA
TTGAAATTTTTGGTGTCTTTGCGGCTGGCTTTTTAATGCTGCCTGTTGGAAGCATTATTTTTGGCAAAATGGCTGACATT
AAGGGGCGTAAAAAGGCAATGATAGTTTCTATCATCTTTATGGCATTTGGTTCTTTTGGTATAGCCTTTTTACCTGATAA
ACAAGCAATTGGAGATATAGCTGTGGTGTTTTTGCTGCTTTTGCGGCTTATTCAAGGCATAGCAGTTGGCGGAGAATTTG
GTATAGTTGCAGCTTATTTAACAGAAATTGCCCCACAAGGCAAAAGAGGCTTTGTATCAAGCTTTCAATATGTTACCATA
ATCGGCGGACAACTTCTTGCGGTTGCAAGCATTAGCCTTTTATTTTTGTTTATAGATGAAAGACAAATGCAAGAATTTGG
TTGGAGAATTTTATTTTTCGTGGGCGGAGTTTTGGCTGTTTTAAGCCTCTTTTTTAGAAGTTTTATACAAGATAATTCTC
ATAAAGTCCTTGAAAATTATGCTGATAGAGGGAGCTTTAAAGCCCTTTTTAAATCCTACAAAGCCCTTTTAATCGTCATA
GGCGTAACGGCTGGTTGCACCATAGGCTTTTATGCAATCACAGTTTATCCTAAGGTATTTATGATAAACAACGGCGTTGA
TACAATCTTAGCCAATAACATTATGCTAGGCTCTTTATTTGTGCTGTGTGTGGCTATACCCTTTATCGGCGCAATTAGCG
ATAAAATAGGCTTTAAAATTTCACTCTTCATTTATCTTGGTTTTTGCCTTGTAGGGACTTATCCGCTTTTTATGGCTTTA
AAAAGCGTAGCATTAAGCGGGTCAAATGAGTTTTTGCTCTTTGTTGTAGTTTGTTTTATGTGTTTTATGTTAAGTTTTTA
CACAGCTGTGGCAGCTATTTCTAAAACCTCACTTTTTCCACCCCAAATTCGCGCACTTGGTGCTGGACTTGGGTGTATGA
TAAGTGTGGGACTTTTTGGCGGAAGCGTGAATTATGTAGCCTTGCAATTTAAAGCACTTGGCATAGAATGGGGCTTTTTT
GTGTATTTTGGCGTTATTGCTTGTATCTCGCTTATTTGCACAGCTTTAATACCAAAGCAAAGAGAACTTGACTAG

Upstream 100 bases:

>100_bases
GAAAAATCCTTAAAACTAAACTAAGGCGATTATCAATTATAGCATAAATTCATGTTGATTTAAAATTTTTTGGCTAAAAT
TCTCAGCAAAAAGGACAAAA

Downstream 100 bases:

>100_bases
AGCGCACTTGCGTGATAAAAAAATTTTTAAAGTAATATTACTTTTGTTTTGTGATTTTTCGAATTTGGCGTTATACTTCA
TCGCTATGTTTTCTTAATTC

Product: alpha-ketoglutarate permease

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MQTTKLSKKAQIHSVIAASSGNLVEWFDFYIYGFAAVYFAHNFSNATSTLFQQIEIFGVFAAGFLMLPVGSIIFGKMADI
KGRKKAMIVSIIFMAFGSFGIAFLPDKQAIGDIAVVFLLLLRLIQGIAVGGEFGIVAAYLTEIAPQGKRGFVSSFQYVTI
IGGQLLAVASISLLFLFIDERQMQEFGWRILFFVGGVLAVLSLFFRSFIQDNSHKVLENYADRGSFKALFKSYKALLIVI
GVTAGCTIGFYAITVYPKVFMINNGVDTILANNIMLGSLFVLCVAIPFIGAISDKIGFKISLFIYLGFCLVGTYPLFMAL
KSVALSGSNEFLLFVVVCFMCFMLSFYTAVAAISKTSLFPPQIRALGAGLGCMISVGLFGGSVNYVALQFKALGIEWGFF
VYFGVIACISLICTALIPKQRELD

Sequences:

>Translated_424_residues
MQTTKLSKKAQIHSVIAASSGNLVEWFDFYIYGFAAVYFAHNFSNATSTLFQQIEIFGVFAAGFLMLPVGSIIFGKMADI
KGRKKAMIVSIIFMAFGSFGIAFLPDKQAIGDIAVVFLLLLRLIQGIAVGGEFGIVAAYLTEIAPQGKRGFVSSFQYVTI
IGGQLLAVASISLLFLFIDERQMQEFGWRILFFVGGVLAVLSLFFRSFIQDNSHKVLENYADRGSFKALFKSYKALLIVI
GVTAGCTIGFYAITVYPKVFMINNGVDTILANNIMLGSLFVLCVAIPFIGAISDKIGFKISLFIYLGFCLVGTYPLFMAL
KSVALSGSNEFLLFVVVCFMCFMLSFYTAVAAISKTSLFPPQIRALGAGLGCMISVGLFGGSVNYVALQFKALGIEWGFF
VYFGVIACISLICTALIPKQRELD
>Mature_424_residues
MQTTKLSKKAQIHSVIAASSGNLVEWFDFYIYGFAAVYFAHNFSNATSTLFQQIEIFGVFAAGFLMLPVGSIIFGKMADI
KGRKKAMIVSIIFMAFGSFGIAFLPDKQAIGDIAVVFLLLLRLIQGIAVGGEFGIVAAYLTEIAPQGKRGFVSSFQYVTI
IGGQLLAVASISLLFLFIDERQMQEFGWRILFFVGGVLAVLSLFFRSFIQDNSHKVLENYADRGSFKALFKSYKALLIVI
GVTAGCTIGFYAITVYPKVFMINNGVDTILANNIMLGSLFVLCVAIPFIGAISDKIGFKISLFIYLGFCLVGTYPLFMAL
KSVALSGSNEFLLFVVVCFMCFMLSFYTAVAAISKTSLFPPQIRALGAGLGCMISVGLFGGSVNYVALQFKALGIEWGFF
VYFGVIACISLICTALIPKQRELD

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=418, Percent_Identity=41.1483253588517, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1790550, Length=373, Percent_Identity=30.0268096514745, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1788292, Length=400, Percent_Identity=28.5, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1789941, Length=213, Percent_Identity=31.4553990610329, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082404, Length=374, Percent_Identity=24.331550802139, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87082231, Length=225, Percent_Identity=24.8888888888889, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46214; Mature: 46214

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCC
>Mature Secondary Structure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CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]