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The map label for this gene is pyk [H]

Identifier: 153952505

GI number: 153952505

Start: 1382967

End: 1384409

Strand: Direct

Name: pyk [H]

Synonym: JJD26997_1566

Alternate gene names: 153952505

Gene position: 1382967-1384409 (Clockwise)

Preceding gene: 153951539

Following gene: 153950987

Centisome position: 74.95

GC content: 30.28

Gene sequence:

>1443_bases
ATGCTTAAAAAAACAAAAATTGTAGCCACAGTAGGTCCAGCAAGTGAAAAGGAAGAAATTCTACGCCAAATGATTATCAA
TGGTGTAAATGTTTTCCGTTTAAACTTTTCTCATGGAACACATGAGTATCATAAAAAAAACTTAGACACCATAAGAAGAG
TAGCTAAAGAATTGCATACTCGTATTGGAATTTTACAAGACATTAGTGGTCCTAAAATTCGTACAGGTGAACTTAAAGAA
CTATTTGAATTAAAAAAGGGTGATAGACTTGATTTTTATCGTGAAGCAATTTTAGGAGAGAAAATTGCTCAAAATCATTA
TAAAATCAGTATTAATCAAAAATCTATTTTAAATATGTTAAAAATTGATGAATATATTTATCTATATGATGGATCAATTC
GTGCTAAAGTAGTAAGTACAGATGATCAAAAAATTGAAACTATTATAGAAAATGATGGTTTTTTAAACTCAAATAAAGGT
ATTAATTTTCCGAATACTAAAATCAATATTGATGTTATTACACAAAAAGATAAAAATGATTTACTTTGGGGTATAAAAAA
TGAAGTAGATTTTTTAGCAATATCTTTCGTACAAAATGCACACGATATTGATGAAGTTCGTGAAATTTTAGCACAAAATA
ACGCAAAAATATCCGTTTTTGCAAAAATTGAAAAATTTGATGCGGTAGAAAATATTGATGAAATTATTAAATCTAGTGAT
GGTATAATGGTTGCAAGAGGAGACTTAGGTATTGAGGTGCCTTATTATAAAGTTCCTAATATCCAAAAAGAAATTATACA
AAAAGCAAACAATGCCTCTAAGCCCGTTATTACTGCGACTCAGATGCTTTTTTCATTAGCCAAAAGCAAAACTGCCACAA
GAGCTGAAATTTCAGATGTTGCAAATGCTGTTTTAGATGGAACAGATGCAGTAATGCTAAGTGAAGAAAGTGCCGTTGGT
ATAGATCCTGCAAATGCTGTTGATATAATGTGCCAAACTATAATAGAAACAGAAAAACGCTATCCATATAACAAATTTAA
TGATTTTAATGATTTAGACAATACAGATAAAATAATGCGTTCTTCAGCTCATCTTGCAACAGATCTTAACGCTGATGCTA
TTTTTTCTTTAACAAGTAGCGGAAAATCTGCTATTAAGATTGCGAGATATCGTCCAAATATAGAAATCATAGCAGTAGGG
CATTCTGAAAAAACCTTAAACTCTTTAAGTATAGTTTGGGGGGTTAATCCTGCGATCTTAGTTAACAAAAGTAATGAACT
TACAGAATTATTAAAAGACTCTGTAAGATCAAGTGTTGAAAAAGGTTTTATGGATGAAGATAAATGCTATCTTCTAACTG
CAGGCTTTCCAACAGGAGTAGAAGGTAGTAGCAATCTTATCCGCATTCTCAATAAAGAGCAAATTGCTTATTATCTACAA
TAA

Upstream 100 bases:

>100_bases
TTACCTAGGAATGGAATTTAACGGAGATAAATTTTCTTCAGAATTATTGTAATCATTTTGTTTCTATTTTTAGTTACAAT
AATTTCAAAAGGAGTTTGCC

Downstream 100 bases:

>100_bases
AACATTAAATTCCTTTGAAAATAAGGAATTTAATGTCCTTATAAATTTTTTAAGTCAAAAGTCGATATAAACTAAAGCCT
CACACAAAGGAGCAAATATG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MLKKTKIVATVGPASEKEEILRQMIINGVNVFRLNFSHGTHEYHKKNLDTIRRVAKELHTRIGILQDISGPKIRTGELKE
LFELKKGDRLDFYREAILGEKIAQNHYKISINQKSILNMLKIDEYIYLYDGSIRAKVVSTDDQKIETIIENDGFLNSNKG
INFPNTKINIDVITQKDKNDLLWGIKNEVDFLAISFVQNAHDIDEVREILAQNNAKISVFAKIEKFDAVENIDEIIKSSD
GIMVARGDLGIEVPYYKVPNIQKEIIQKANNASKPVITATQMLFSLAKSKTATRAEISDVANAVLDGTDAVMLSEESAVG
IDPANAVDIMCQTIIETEKRYPYNKFNDFNDLDNTDKIMRSSAHLATDLNADAIFSLTSSGKSAIKIARYRPNIEIIAVG
HSEKTLNSLSIVWGVNPAILVNKSNELTELLKDSVRSSVEKGFMDEDKCYLLTAGFPTGVEGSSNLIRILNKEQIAYYLQ

Sequences:

>Translated_480_residues
MLKKTKIVATVGPASEKEEILRQMIINGVNVFRLNFSHGTHEYHKKNLDTIRRVAKELHTRIGILQDISGPKIRTGELKE
LFELKKGDRLDFYREAILGEKIAQNHYKISINQKSILNMLKIDEYIYLYDGSIRAKVVSTDDQKIETIIENDGFLNSNKG
INFPNTKINIDVITQKDKNDLLWGIKNEVDFLAISFVQNAHDIDEVREILAQNNAKISVFAKIEKFDAVENIDEIIKSSD
GIMVARGDLGIEVPYYKVPNIQKEIIQKANNASKPVITATQMLFSLAKSKTATRAEISDVANAVLDGTDAVMLSEESAVG
IDPANAVDIMCQTIIETEKRYPYNKFNDFNDLDNTDKIMRSSAHLATDLNADAIFSLTSSGKSAIKIARYRPNIEIIAVG
HSEKTLNSLSIVWGVNPAILVNKSNELTELLKDSVRSSVEKGFMDEDKCYLLTAGFPTGVEGSSNLIRILNKEQIAYYLQ
>Mature_480_residues
MLKKTKIVATVGPASEKEEILRQMIINGVNVFRLNFSHGTHEYHKKNLDTIRRVAKELHTRIGILQDISGPKIRTGELKE
LFELKKGDRLDFYREAILGEKIAQNHYKISINQKSILNMLKIDEYIYLYDGSIRAKVVSTDDQKIETIIENDGFLNSNKG
INFPNTKINIDVITQKDKNDLLWGIKNEVDFLAISFVQNAHDIDEVREILAQNNAKISVFAKIEKFDAVENIDEIIKSSD
GIMVARGDLGIEVPYYKVPNIQKEIIQKANNASKPVITATQMLFSLAKSKTATRAEISDVANAVLDGTDAVMLSEESAVG
IDPANAVDIMCQTIIETEKRYPYNKFNDFNDLDNTDKIMRSSAHLATDLNADAIFSLTSSGKSAIKIARYRPNIEIIAVG
HSEKTLNSLSIVWGVNPAILVNKSNELTELLKDSVRSSVEKGFMDEDKCYLLTAGFPTGVEGSSNLIRILNKEQIAYYLQ

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=491, Percent_Identity=36.4562118126273, Blast_Score=291, Evalue=7e-79,
Organism=Homo sapiens, GI33286422, Length=498, Percent_Identity=36.7469879518072, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI33286420, Length=498, Percent_Identity=36.7469879518072, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI32967597, Length=492, Percent_Identity=34.349593495935, Blast_Score=276, Evalue=4e-74,
Organism=Homo sapiens, GI10835121, Length=492, Percent_Identity=34.349593495935, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=39.4463667820069, Blast_Score=205, Evalue=8e-53,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=39.4463667820069, Blast_Score=205, Evalue=8e-53,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=35.0210970464135, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=35.0210970464135, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=34.2592592592593, Blast_Score=129, Evalue=6e-30,
Organism=Escherichia coli, GI1787965, Length=425, Percent_Identity=40, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=35.7588357588358, Blast_Score=265, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17544584, Length=486, Percent_Identity=37.4485596707819, Blast_Score=290, Evalue=9e-79,
Organism=Caenorhabditis elegans, GI71984406, Length=491, Percent_Identity=35.8452138492872, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71984413, Length=491, Percent_Identity=35.8452138492872, Blast_Score=276, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17506829, Length=491, Percent_Identity=35.8452138492872, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17506831, Length=491, Percent_Identity=35.8452138492872, Blast_Score=275, Evalue=4e-74,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=33.7448559670782, Blast_Score=267, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6324923, Length=486, Percent_Identity=33.1275720164609, Blast_Score=248, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=37.4493927125506, Blast_Score=308, Evalue=5e-84,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=37.4493927125506, Blast_Score=307, Evalue=9e-84,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=34.1463414634146, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24581235, Length=484, Percent_Identity=29.5454545454545, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24646914, Length=270, Percent_Identity=35.1851851851852, Blast_Score=148, Evalue=8e-36,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 53777; Mature: 53777

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00659 GLYCOSYL_HYDROL_F5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKTKIVATVGPASEKEEILRQMIINGVNVFRLNFSHGTHEYHKKNLDTIRRVAKELHT
CCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCHHHHHHHHHHHHH
RIGILQDISGPKIRTGELKELFELKKGDRLDFYREAILGEKIAQNHYKISINQKSILNML
HHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHH
KIDEYIYLYDGSIRAKVVSTDDQKIETIIENDGFLNSNKGINFPNTKINIDVITQKDKND
HHCCEEEEECCCEEEEEECCCCHHHHHHHCCCCEECCCCCCCCCCCEEEEEEEEECCCCC
LLWGIKNEVDFLAISFVQNAHDIDEVREILAQNNAKISVFAKIEKFDAVENIDEIIKSSD
CEEECCCCCCEEEEEHHHCCCCHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHCCC
GIMVARGDLGIEVPYYKVPNIQKEIIQKANNASKPVITATQMLFSLAKSKTATRAEISDV
CEEEEECCCCEECCCEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
ANAVLDGTDAVMLSEESAVGIDPANAVDIMCQTIIETEKRYPYNKFNDFNDLDNTDKIMR
HHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
SSAHLATDLNADAIFSLTSSGKSAIKIARYRPNIEIIAVGHSEKTLNSLSIVWGVNPAIL
HCCHHHCCCCCCCEEEECCCCCCEEEEEEECCCEEEEEECCCCCCHHCEEEEECCCCEEE
VNKSNELTELLKDSVRSSVEKGFMDEDKCYLLTAGFPTGVEGSSNLIRILNKEQIAYYLQ
EECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCEEEEEC
>Mature Secondary Structure
MLKKTKIVATVGPASEKEEILRQMIINGVNVFRLNFSHGTHEYHKKNLDTIRRVAKELHT
CCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCHHHHHHHHHHHHH
RIGILQDISGPKIRTGELKELFELKKGDRLDFYREAILGEKIAQNHYKISINQKSILNML
HHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHH
KIDEYIYLYDGSIRAKVVSTDDQKIETIIENDGFLNSNKGINFPNTKINIDVITQKDKND
HHCCEEEEECCCEEEEEECCCCHHHHHHHCCCCEECCCCCCCCCCCEEEEEEEEECCCCC
LLWGIKNEVDFLAISFVQNAHDIDEVREILAQNNAKISVFAKIEKFDAVENIDEIIKSSD
CEEECCCCCCEEEEEHHHCCCCHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHCCC
GIMVARGDLGIEVPYYKVPNIQKEIIQKANNASKPVITATQMLFSLAKSKTATRAEISDV
CEEEEECCCCEECCCEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
ANAVLDGTDAVMLSEESAVGIDPANAVDIMCQTIIETEKRYPYNKFNDFNDLDNTDKIMR
HHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
SSAHLATDLNADAIFSLTSSGKSAIKIARYRPNIEIIAVGHSEKTLNSLSIVWGVNPAIL
HCCHHHCCCCCCCEEEECCCCCCEEEEEEECCCEEEEEECCCCCCHHCEEEEECCCCEEE
VNKSNELTELLKDSVRSSVEKGFMDEDKCYLLTAGFPTGVEGSSNLIRILNKEQIAYYLQ
EECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA