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The map label for this gene is iaaA [H]

Identifier: 153952392

GI number: 153952392

Start: 1576810

End: 1577841

Strand: Direct

Name: iaaA [H]

Synonym: JJD26997_1791

Alternate gene names: 153952392

Gene position: 1576810-1577841 (Clockwise)

Preceding gene: 153952316

Following gene: 153952112

Centisome position: 85.46

GC content: 42.54

Gene sequence:

>1032_bases
ATGAAAATTCTAAACATAGGTGCAAAAGCCTTTTTTTTAATGGCAAGTTTAGGCTGTTTAAATACCGCCCTAGTTGCTAA
GGACATCAAACCCATCATCGTCATACACGGAGGCACAAGTGGTCTAGGTTTAAGCAAGGAAGAATTTGCCAAACGCGAAG
TTGTGATGAAGCAAAGTTTAAAGGCAGGGCAGAAAATTTTAGAACAGGGTGGAAGTGCTACTGATGCAGTGATTGCAACG
ATTAAGGTTATGGAAGATAGCGAAGAATTTAATGCAGGAAAGGGAGCAGTATTTACAGCGGATGGTTTTAATGAGCTTGA
TGCTTCCTTGATGGAAGGTAAGGGCAAAAAAGCAGGAGCCATAGCTATGGCAAGAACGATTAAAAATCCTATCGAAGCGG
CTAGAGTAGTGATGGAAAAAACGCCTCACACACTCATAGCAGGAGAGGGAGCGGACAAGTTAGCTAAAGAAAATGAACTT
GAAATTGTGTCGCAAAAATACTTTTACACCCCGCACCGCTATAAGCAACTTGAAGATGCCAAAAAGAGCAAAGAAATTCT
ACTCGATAGTGATAAGGCAAAGGCACATTTGGGTAATTATACTGAGCCTTATTTGGGTACTGTAGGTGCCATAGCCTTAG
ACAAGGAAGGGAATTTAGCCGCTGGGACAAGCACGGGTGGCACGACTAATAAAATGACAGGACGCATAGGCGATTCGCCT
ATTATCGGGGCGGGTAATTACGCAGATAATGATTCTGTGGCTATTTCTTGCACAGGCACGGGCGATATTTTCATAAGATC
TATTGCAGCTTATAAAGTGTCCGCTCTTTATAAATATAAAAAATTATCCGTGCAAAAAGCAGCCGAGCAAAGTATAGATG
AAGTGGCGAAATTAGGCGGAACGGGTGGCATTATCTCTATCGATAAAAACGGCAATGTAGGCTATGCTTGGACTAAGGAT
AAACTAGGAATGTATCACGGCGAAGCCAGGGTGGGTCAAGAACCTAGGGTCTTTTTTCCCGTTGCTAAGTAA

Upstream 100 bases:

>100_bases
GTAACATTCTTTCAATTAAAATTTAAGAAATATTAAATTTTAAATAGTTTATTATGAATAATAAAATATTAATATTTTAT
AAGCTAAACAAGGAGTAAAA

Downstream 100 bases:

>100_bases
GCACACAGAAGTGCAAATTAGCCTAAGCCTCGAGTTTTAGGACAAGAATATTGTCCTAAACACCTTAGCGTTAGTTAAAT
TTTTATCCTAAGTCTTAAAA

Product: putative L-asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKILNIGAKAFFLMASLGCLNTALVAKDIKPIIVIHGGTSGLGLSKEEFAKREVVMKQSLKAGQKILEQGGSATDAVIAT
IKVMEDSEEFNAGKGAVFTADGFNELDASLMEGKGKKAGAIAMARTIKNPIEAARVVMEKTPHTLIAGEGADKLAKENEL
EIVSQKYFYTPHRYKQLEDAKKSKEILLDSDKAKAHLGNYTEPYLGTVGAIALDKEGNLAAGTSTGGTTNKMTGRIGDSP
IIGAGNYADNDSVAISCTGTGDIFIRSIAAYKVSALYKYKKLSVQKAAEQSIDEVAKLGGTGGIISIDKNGNVGYAWTKD
KLGMYHGEARVGQEPRVFFPVAK

Sequences:

>Translated_343_residues
MKILNIGAKAFFLMASLGCLNTALVAKDIKPIIVIHGGTSGLGLSKEEFAKREVVMKQSLKAGQKILEQGGSATDAVIAT
IKVMEDSEEFNAGKGAVFTADGFNELDASLMEGKGKKAGAIAMARTIKNPIEAARVVMEKTPHTLIAGEGADKLAKENEL
EIVSQKYFYTPHRYKQLEDAKKSKEILLDSDKAKAHLGNYTEPYLGTVGAIALDKEGNLAAGTSTGGTTNKMTGRIGDSP
IIGAGNYADNDSVAISCTGTGDIFIRSIAAYKVSALYKYKKLSVQKAAEQSIDEVAKLGGTGGIISIDKNGNVGYAWTKD
KLGMYHGEARVGQEPRVFFPVAK
>Mature_343_residues
MKILNIGAKAFFLMASLGCLNTALVAKDIKPIIVIHGGTSGLGLSKEEFAKREVVMKQSLKAGQKILEQGGSATDAVIAT
IKVMEDSEEFNAGKGAVFTADGFNELDASLMEGKGKKAGAIAMARTIKNPIEAARVVMEKTPHTLIAGEGADKLAKENEL
EIVSQKYFYTPHRYKQLEDAKKSKEILLDSDKAKAHLGNYTEPYLGTVGAIALDKEGNLAAGTSTGGTTNKMTGRIGDSP
IIGAGNYADNDSVAISCTGTGDIFIRSIAAYKVSALYKYKKLSVQKAAEQSIDEVAKLGGTGGIISIDKNGNVGYAWTKD
KLGMYHGEARVGQEPRVFFPVAK

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=295, Percent_Identity=38.6440677966102, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI145275200, Length=295, Percent_Identity=38.6440677966102, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI98991769, Length=268, Percent_Identity=30.5970149253731, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI285002251, Length=290, Percent_Identity=30.3448275862069, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI285002253, Length=290, Percent_Identity=28.9655172413793, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1787050, Length=311, Percent_Identity=45.6591639871383, Blast_Score=260, Evalue=9e-71,
Organism=Caenorhabditis elegans, GI17569239, Length=241, Percent_Identity=28.2157676348548, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI18921183, Length=304, Percent_Identity=37.171052631579, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI21357893, Length=266, Percent_Identity=31.5789473684211, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24652135, Length=239, Percent_Identity=33.0543933054393, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI221330102, Length=242, Percent_Identity=32.6446280991736, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 36402; Mature: 36402

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILNIGAKAFFLMASLGCLNTALVAKDIKPIIVIHGGTSGLGLSKEEFAKREVVMKQSL
CEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
KAGQKILEQGGSATDAVIATIKVMEDSEEFNAGKGAVFTADGFNELDASLMEGKGKKAGA
HHHHHHHHCCCCCCHHHEEEEEEEECCHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCHH
IAMARTIKNPIEAARVVMEKTPHTLIAGEGADKLAKENELEIVSQKYFYTPHRYKQLEDA
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCHHEEHHHHCCCCHHHHHHHHH
KKSKEILLDSDKAKAHLGNYTEPYLGTVGAIALDKEGNLAAGTSTGGTTNKMTGRIGDSP
HHHHHEEEECCCHHHHCCCCCCCHHCCCEEEEECCCCCEEECCCCCCCCCCEECCCCCCC
IIGAGNYADNDSVAISCTGTGDIFIRSIAAYKVSALYKYKKLSVQKAAEQSIDEVAKLGG
EEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TGGIISIDKNGNVGYAWTKDKLGMYHGEARVGQEPRVFFPVAK
CCCEEEEECCCCEEEEEECCCCCCEECCHHCCCCCCEEEECCC
>Mature Secondary Structure
MKILNIGAKAFFLMASLGCLNTALVAKDIKPIIVIHGGTSGLGLSKEEFAKREVVMKQSL
CEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
KAGQKILEQGGSATDAVIATIKVMEDSEEFNAGKGAVFTADGFNELDASLMEGKGKKAGA
HHHHHHHHCCCCCCHHHEEEEEEEECCHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCHH
IAMARTIKNPIEAARVVMEKTPHTLIAGEGADKLAKENELEIVSQKYFYTPHRYKQLEDA
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCHHEEHHHHCCCCHHHHHHHHH
KKSKEILLDSDKAKAHLGNYTEPYLGTVGAIALDKEGNLAAGTSTGGTTNKMTGRIGDSP
HHHHHEEEECCCHHHHCCCCCCCHHCCCEEEEECCCCCEEECCCCCCCCCCEECCCCCCC
IIGAGNYADNDSVAISCTGTGDIFIRSIAAYKVSALYKYKKLSVQKAAEQSIDEVAKLGG
EEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TGGIISIDKNGNVGYAWTKDKLGMYHGEARVGQEPRVFFPVAK
CCCEEEEECCCCEEEEEECCCCCCEECCHHCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]