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The map label for this gene is trmE [H]

Identifier: 153952344

GI number: 153952344

Start: 742950

End: 744278

Strand: Direct

Name: trmE [H]

Synonym: JJD26997_0825

Alternate gene names: 153952344

Gene position: 742950-744278 (Clockwise)

Preceding gene: 153951735

Following gene: 153951583

Centisome position: 40.27

GC content: 31.83

Gene sequence:

>1329_bases
ATGAGTGATACTATAGCGGCTATTGCCACAGCTCATGGTGTGGGTTCGATTTCTATTGTAAGATTAAGCGGTGAAAGGGC
TTTAGAATTTGCCTTAAGGTTTAGTCGAAAAACCAAACTCACCCCCCGCCATGCTACTTTTACAAAACTTTTTAATCAAA
ACAATGAAATCATCGATGAAGCTATAATGATATATTTTAAAGCCCCTTATAGTTTTACAGGCGAAGATATAGTGGAGTTT
CAAACTCATGGGGGATTTAGCGTGAGTGAAGTTTTACTCGAAGAACTTGTGAGTTTAGGAGCAAGATTTGCTTTAGCGGG
TGAATTTAGCAAAAGAGCGTGTTTAAATGGCAAAATGACTCCTTTAAAAGCCTTAAATATACAAGATCTCATACTTTCAA
AATCTGCACTTGCTGCCAAAATCATAGCACGCAACATGCAAGGAAATTTAGGAGAACTTTTAGAAAAAATTCGCACAGAT
TTGGTTAAAACCTTAGCTTTTGTAGAAACTAGCATTGATTATGCAGACGATGACTTGCCTTCTGATTTATTAGAAAAAAT
TTCTACTATGTGTGAAGAAAATTCTAAAATTTTAAAAGAAATTTATACACTTTCTCAAAGCAAAAAAGGACTTATAGAAG
GTTTTAAAATCGCTATTATAGGTAAGCCAAATGTAGGAAAATCTTCACTTTTGAATGCCTTGCTTTCTTATGAAAGAGCT
ATTGTTTCAGATATAGCAGGTACCACTCGTGATACTATAGAAGAGAGTTTTAAACTAGGAACGCATTTATTGCGTATTAT
AGATACAGCGGGTATTAGAGAAAGTAAAGATACCATAGAACAAATTGGCGTAGCCTTAAGCAAAAAAAGTTTAGAAGATG
CAGATATTATTTTGGCAGTCTTTGATGCTTCAAGAGTACAAGATAAAGAAGATGAGAAAATTTTTGAATTGCTAGCAAAT
ACTGATAAAAAAATCTTTTGGATTTTAAATAAAAGTGATTTAGAAAATGTTTTCAAAAATACTCGAAATAAAAATTTTAT
CAAGCTTAGTGCTCAAAAAGACATAGCTTTGTTAAAAGAAGAATTGCAAAATTATCTTAATTCTTTTGACAGTGAAGGTA
TTATGGTAAGTTCTTTAGATCTTATCAATGCTTGTAAAATTTCAAGTGAAGCGATTTTTAGAGCTAAGGAGCTTTTAGAG
GAAAGTTCTTTAGAACTTTTTGCTTTTGAGCTTAATTTAGCTATTAATGAACTTGCAAGATTTACCAAAGATTTTCAAAG
AGATGAAATTTTAGACGAAATGTTTGGTAATTTTTGTTTAGGAAAATGA

Upstream 100 bases:

>100_bases
GCTTTAGAGCAGTTAAGAGCTGAATTTCCAGATAAGTATGTAGGAATTAAGCAAAATAATGATCAAAGATTTGTTGTAAT
AAACGACTTTTTCAAAAAAG

Downstream 100 bases:

>100_bases
AAGGATAAAAATGGATAAAGAAACCATAAAAGCACATAAAATCAGCGATGAAGAATATACACAAATTTTAGAAATTTTAG
GACGTGAGCCAAATTTATTA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSDTIAAIATAHGVGSISIVRLSGERALEFALRFSRKTKLTPRHATFTKLFNQNNEIIDEAIMIYFKAPYSFTGEDIVEF
QTHGGFSVSEVLLEELVSLGARFALAGEFSKRACLNGKMTPLKALNIQDLILSKSALAAKIIARNMQGNLGELLEKIRTD
LVKTLAFVETSIDYADDDLPSDLLEKISTMCEENSKILKEIYTLSQSKKGLIEGFKIAIIGKPNVGKSSLLNALLSYERA
IVSDIAGTTRDTIEESFKLGTHLLRIIDTAGIRESKDTIEQIGVALSKKSLEDADIILAVFDASRVQDKEDEKIFELLAN
TDKKIFWILNKSDLENVFKNTRNKNFIKLSAQKDIALLKEELQNYLNSFDSEGIMVSSLDLINACKISSEAIFRAKELLE
ESSLELFAFELNLAINELARFTKDFQRDEILDEMFGNFCLGK

Sequences:

>Translated_442_residues
MSDTIAAIATAHGVGSISIVRLSGERALEFALRFSRKTKLTPRHATFTKLFNQNNEIIDEAIMIYFKAPYSFTGEDIVEF
QTHGGFSVSEVLLEELVSLGARFALAGEFSKRACLNGKMTPLKALNIQDLILSKSALAAKIIARNMQGNLGELLEKIRTD
LVKTLAFVETSIDYADDDLPSDLLEKISTMCEENSKILKEIYTLSQSKKGLIEGFKIAIIGKPNVGKSSLLNALLSYERA
IVSDIAGTTRDTIEESFKLGTHLLRIIDTAGIRESKDTIEQIGVALSKKSLEDADIILAVFDASRVQDKEDEKIFELLAN
TDKKIFWILNKSDLENVFKNTRNKNFIKLSAQKDIALLKEELQNYLNSFDSEGIMVSSLDLINACKISSEAIFRAKELLE
ESSLELFAFELNLAINELARFTKDFQRDEILDEMFGNFCLGK
>Mature_441_residues
SDTIAAIATAHGVGSISIVRLSGERALEFALRFSRKTKLTPRHATFTKLFNQNNEIIDEAIMIYFKAPYSFTGEDIVEFQ
THGGFSVSEVLLEELVSLGARFALAGEFSKRACLNGKMTPLKALNIQDLILSKSALAAKIIARNMQGNLGELLEKIRTDL
VKTLAFVETSIDYADDDLPSDLLEKISTMCEENSKILKEIYTLSQSKKGLIEGFKIAIIGKPNVGKSSLLNALLSYERAI
VSDIAGTTRDTIEESFKLGTHLLRIIDTAGIRESKDTIEQIGVALSKKSLEDADIILAVFDASRVQDKEDEKIFELLANT
DKKIFWILNKSDLENVFKNTRNKNFIKLSAQKDIALLKEELQNYLNSFDSEGIMVSSLDLINACKISSEAIFRAKELLEE
SSLELFAFELNLAINELARFTKDFQRDEILDEMFGNFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=465, Percent_Identity=31.6129032258064, Blast_Score=189, Evalue=6e-48,
Organism=Homo sapiens, GI193082988, Length=494, Percent_Identity=30.1619433198381, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI193082993, Length=456, Percent_Identity=30.9210526315789, Blast_Score=175, Evalue=1e-43,
Organism=Escherichia coli, GI2367268, Length=455, Percent_Identity=34.9450549450549, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI87082120, Length=121, Percent_Identity=35.5371900826446, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17507259, Length=450, Percent_Identity=29.1111111111111, Blast_Score=179, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6323665, Length=491, Percent_Identity=30.9572301425662, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI45550813, Length=473, Percent_Identity=28.5412262156448, Blast_Score=163, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49299; Mature: 49168

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTIAAIATAHGVGSISIVRLSGERALEFALRFSRKTKLTPRHATFTKLFNQNNEIIDE
CCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHH
AIMIYFKAPYSFTGEDIVEFQTHGGFSVSEVLLEELVSLGARFALAGEFSKRACLNGKMT
HHHHEEECCCCCCCCHHHEEECCCCCCHHHHHHHHHHHHCCCEEECCCHHHHHHCCCCCC
PLKALNIQDLILSKSALAAKIIARNMQGNLGELLEKIRTDLVKTLAFVETSIDYADDDLP
CHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SDLLEKISTMCEENSKILKEIYTLSQSKKGLIEGFKIAIIGKPNVGKSSLLNALLSYERA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEEEECCCCCHHHHHHHHHHHHHH
IVSDIAGTTRDTIEESFKLGTHLLRIIDTAGIRESKDTIEQIGVALSKKSLEDADIILAV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
FDASRVQDKEDEKIFELLANTDKKIFWILNKSDLENVFKNTRNKNFIKLSAQKDIALLKE
ECCHHCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCCCCEEEEECHHHHHHHHH
ELQNYLNSFDSEGIMVSSLDLINACKISSEAIFRAKELLEESSLELFAFELNLAINELAR
HHHHHHHHCCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHH
FTKDFQRDEILDEMFGNFCLGK
HHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDTIAAIATAHGVGSISIVRLSGERALEFALRFSRKTKLTPRHATFTKLFNQNNEIIDE
CCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHH
AIMIYFKAPYSFTGEDIVEFQTHGGFSVSEVLLEELVSLGARFALAGEFSKRACLNGKMT
HHHHEEECCCCCCCCHHHEEECCCCCCHHHHHHHHHHHHCCCEEECCCHHHHHHCCCCCC
PLKALNIQDLILSKSALAAKIIARNMQGNLGELLEKIRTDLVKTLAFVETSIDYADDDLP
CHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SDLLEKISTMCEENSKILKEIYTLSQSKKGLIEGFKIAIIGKPNVGKSSLLNALLSYERA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEEEECCCCCHHHHHHHHHHHHHH
IVSDIAGTTRDTIEESFKLGTHLLRIIDTAGIRESKDTIEQIGVALSKKSLEDADIILAV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
FDASRVQDKEDEKIFELLANTDKKIFWILNKSDLENVFKNTRNKNFIKLSAQKDIALLKE
ECCHHCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCCCCEEEEECHHHHHHHHH
ELQNYLNSFDSEGIMVSSLDLINACKISSEAIFRAKELLEESSLELFAFELNLAINELAR
HHHHHHHHCCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHH
FTKDFQRDEILDEMFGNFCLGK
HHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA