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The map label for this gene is glcD [H]

Identifier: 153952228

GI number: 153952228

Start: 461209

End: 462591

Strand: Direct

Name: glcD [H]

Synonym: JJD26997_0517

Alternate gene names: 153952228

Gene position: 461209-462591 (Clockwise)

Preceding gene: 153951120

Following gene: 153951615

Centisome position: 25.0

GC content: 34.2

Gene sequence:

>1383_bases
ATGAAAAAAGAATTTGAACAATATTTTAAACGATTTTTAGGAGAAGAAAACGCTTATTTTGATGAAATTCACAAAAGAGC
TTATAGTTACGATGCGACAAAAAAGCATTATTTACCTGATGGGGTTCTTTTTCCAAGAAATGAAGAAGATATTGCTCAAA
TTTTAAAATTTTGTAATGAAAATAATATCATAGTCATACCGCGAGGCTCAGGATCTGGTTTTACAGGAGGAGCTTTAGCT
GTTAATGGCGGGGTGGTGCTTGCTTTTGAAAAGCATATGAATAAAATTTTAGAAATTGATCTTGAAAATTTAGTGGCTGT
TGTACAACCTGGGGTTATTAATATACATTTACAAAAAGAAGTGGCTAAATACGGACTTTTTTACCCGCCTGACCCTGCTA
GCATGGAGTATTCTAGCTTAGGGGGTAATGTAAGTGAAAATGCTGGGGGAATGAGAGCTGCAAAATATGGCATTACAAAA
GATTATGTGATGGCTTTAAGGGCTGTTTTGCCTAGCGGAGAAATTATTCGCGCAGGAAAACGGACTATTAAAGATGTGGC
AGGTTATAATTTGGCAGGAATTTTAATCGCTAGTGAAGGTTCTTTGGCAGTTTTAAGTGAGCTTACTTTAAAATTAATAC
CTTTACCTAAATTCAAAAAAACTGCTTTAGCTATTTTTCCAAGTGTAAAAAGTGCTATGAACGCGGTTTATAAAAGTCTC
GCTAGTGGTGTAAGTCCTGTATCTATGGAATTTTTAGACAATCTTAGTATTAGAGCGGTTGAGAGTAAATTTAACAAAGG
TTTGCCTATAGAAGCGGGCGCTATTTTGATCGCTGATGTGGATGGTAATGTTAAAGAGGCTATTGATGAGGATTTAAGAA
ATTTAGAACATTATTTTTTAGAAGCTGGCGCTAGCGAATTTAAAATAGCAAAAGATGAACAAGAAACCGCAGATATTTGG
TTTGCTAGAAGAAATTGTTCTCAAAGTATTGCCATGTATGGAACTTTAAAACTTAATGAAGATATTACAGTTCCGCGTTC
TAAGCTACCTGCTTTGCTTGAAGGTATAGATGAAATTTCTAAAAAATATGGTTTTAAAATCCCTTGTTTTGGTCATACAG
GGGATGGAAATGTGCATACTAATGTTATGGTTCCTGATAAAAATGATAAAGAGCAGGTGAAAAAAGGCTATGAAGCTGTG
GAAGAGATTTTTAAATTAACAGTGAAACTTGGAGGAACTTTAAGCGGAGAGCATGGTATCGGACTCTCTAAAGCACCTTT
TATGAATTTAGCTTTTTCTGAAGCCGAAATGAATTTAATGAGAAATATTAAAAAAGCTTTTGATCCAAATAATATACTAA
ATCCTTTTAAAATGGGACTTTAG

Upstream 100 bases:

>100_bases
GTGGATATAAAAGTAATTTTTTTGATTTTAACTCTCAAGAAATAGGAAATTATTATAGATATTATGATGCTTTGATTAAT
CTTCCTAAGGTGCAATAAAC

Downstream 100 bases:

>100_bases
AAGGTGAAAAATTTTTTTACTATTTTGCTAAATTTGCGTGATAAGGAAATTTTAAATTATGCAGCAGCACTTAGTTTTTA
TACTGTATTATCTTTAATAC

Product: glycolate oxidase, subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MKKEFEQYFKRFLGEENAYFDEIHKRAYSYDATKKHYLPDGVLFPRNEEDIAQILKFCNENNIIVIPRGSGSGFTGGALA
VNGGVVLAFEKHMNKILEIDLENLVAVVQPGVINIHLQKEVAKYGLFYPPDPASMEYSSLGGNVSENAGGMRAAKYGITK
DYVMALRAVLPSGEIIRAGKRTIKDVAGYNLAGILIASEGSLAVLSELTLKLIPLPKFKKTALAIFPSVKSAMNAVYKSL
ASGVSPVSMEFLDNLSIRAVESKFNKGLPIEAGAILIADVDGNVKEAIDEDLRNLEHYFLEAGASEFKIAKDEQETADIW
FARRNCSQSIAMYGTLKLNEDITVPRSKLPALLEGIDEISKKYGFKIPCFGHTGDGNVHTNVMVPDKNDKEQVKKGYEAV
EEIFKLTVKLGGTLSGEHGIGLSKAPFMNLAFSEAEMNLMRNIKKAFDPNNILNPFKMGL

Sequences:

>Translated_460_residues
MKKEFEQYFKRFLGEENAYFDEIHKRAYSYDATKKHYLPDGVLFPRNEEDIAQILKFCNENNIIVIPRGSGSGFTGGALA
VNGGVVLAFEKHMNKILEIDLENLVAVVQPGVINIHLQKEVAKYGLFYPPDPASMEYSSLGGNVSENAGGMRAAKYGITK
DYVMALRAVLPSGEIIRAGKRTIKDVAGYNLAGILIASEGSLAVLSELTLKLIPLPKFKKTALAIFPSVKSAMNAVYKSL
ASGVSPVSMEFLDNLSIRAVESKFNKGLPIEAGAILIADVDGNVKEAIDEDLRNLEHYFLEAGASEFKIAKDEQETADIW
FARRNCSQSIAMYGTLKLNEDITVPRSKLPALLEGIDEISKKYGFKIPCFGHTGDGNVHTNVMVPDKNDKEQVKKGYEAV
EEIFKLTVKLGGTLSGEHGIGLSKAPFMNLAFSEAEMNLMRNIKKAFDPNNILNPFKMGL
>Mature_460_residues
MKKEFEQYFKRFLGEENAYFDEIHKRAYSYDATKKHYLPDGVLFPRNEEDIAQILKFCNENNIIVIPRGSGSGFTGGALA
VNGGVVLAFEKHMNKILEIDLENLVAVVQPGVINIHLQKEVAKYGLFYPPDPASMEYSSLGGNVSENAGGMRAAKYGITK
DYVMALRAVLPSGEIIRAGKRTIKDVAGYNLAGILIASEGSLAVLSELTLKLIPLPKFKKTALAIFPSVKSAMNAVYKSL
ASGVSPVSMEFLDNLSIRAVESKFNKGLPIEAGAILIADVDGNVKEAIDEDLRNLEHYFLEAGASEFKIAKDEQETADIW
FARRNCSQSIAMYGTLKLNEDITVPRSKLPALLEGIDEISKKYGFKIPCFGHTGDGNVHTNVMVPDKNDKEQVKKGYEAV
EEIFKLTVKLGGTLSGEHGIGLSKAPFMNLAFSEAEMNLMRNIKKAFDPNNILNPFKMGL

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=469, Percent_Identity=30.7036247334755, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI37595754, Length=492, Percent_Identity=29.2682926829268, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI119964728, Length=422, Percent_Identity=25.1184834123223, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI4501993, Length=248, Percent_Identity=27.8225806451613, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1789351, Length=416, Percent_Identity=37.2596153846154, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1787977, Length=532, Percent_Identity=26.6917293233083, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI48994907, Length=438, Percent_Identity=23.7442922374429, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI48994914, Length=165, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71992373, Length=459, Percent_Identity=29.8474945533769, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI71992381, Length=459, Percent_Identity=28.7581699346405, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17534361, Length=463, Percent_Identity=28.2937365010799, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI71992392, Length=363, Percent_Identity=30.5785123966942, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17556096, Length=455, Percent_Identity=25.9340659340659, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320027, Length=441, Percent_Identity=28.7981859410431, Blast_Score=186, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6320023, Length=476, Percent_Identity=26.890756302521, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6320764, Length=484, Percent_Identity=27.4793388429752, Blast_Score=155, Evalue=9e-39,
Organism=Drosophila melanogaster, GI18921117, Length=430, Percent_Identity=27.6744186046512, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24639277, Length=430, Percent_Identity=27.6744186046512, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24639275, Length=430, Percent_Identity=27.6744186046512, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24653753, Length=230, Percent_Identity=28.2608695652174, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 50560; Mature: 50560

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEFEQYFKRFLGEENAYFDEIHKRAYSYDATKKHYLPDGVLFPRNEEDIAQILKFCNE
CCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
NNIIVIPRGSGSGFTGGALAVNGGVVLAFEKHMNKILEIDLENLVAVVQPGVINIHLQKE
CCEEEEECCCCCCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHH
VAKYGLFYPPDPASMEYSSLGGNVSENAGGMRAAKYGITKDYVMALRAVLPSGEIIRAGK
HHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCCEEHHHH
RTIKDVAGYNLAGILIASEGSLAVLSELTLKLIPLPKFKKTALAIFPSVKSAMNAVYKSL
HHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASGVSPVSMEFLDNLSIRAVESKFNKGLPIEAGAILIADVDGNVKEAIDEDLRNLEHYFL
HCCCCCEEHHHHHCCCCEEHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
EAGASEFKIAKDEQETADIWFARRNCSQSIAMYGTLKLNEDITVPRSKLPALLEGIDEIS
HCCCCCCEECCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHH
KKYGFKIPCFGHTGDGNVHTNVMVPDKNDKEQVKKGYEAVEEIFKLTVKLGGTLSGEHGI
HHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCC
GLSKAPFMNLAFSEAEMNLMRNIKKAFDPNNILNPFKMGL
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure
MKKEFEQYFKRFLGEENAYFDEIHKRAYSYDATKKHYLPDGVLFPRNEEDIAQILKFCNE
CCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
NNIIVIPRGSGSGFTGGALAVNGGVVLAFEKHMNKILEIDLENLVAVVQPGVINIHLQKE
CCEEEEECCCCCCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHH
VAKYGLFYPPDPASMEYSSLGGNVSENAGGMRAAKYGITKDYVMALRAVLPSGEIIRAGK
HHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCCEEHHHH
RTIKDVAGYNLAGILIASEGSLAVLSELTLKLIPLPKFKKTALAIFPSVKSAMNAVYKSL
HHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASGVSPVSMEFLDNLSIRAVESKFNKGLPIEAGAILIADVDGNVKEAIDEDLRNLEHYFL
HCCCCCEEHHHHHCCCCEEHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
EAGASEFKIAKDEQETADIWFARRNCSQSIAMYGTLKLNEDITVPRSKLPALLEGIDEIS
HCCCCCCEECCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHH
KKYGFKIPCFGHTGDGNVHTNVMVPDKNDKEQVKKGYEAVEEIFKLTVKLGGTLSGEHGI
HHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCC
GLSKAPFMNLAFSEAEMNLMRNIKKAFDPNNILNPFKMGL
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]