Click here to switch to the map view.

The map label for this gene is dnaN [H]

Identifier: 153952198

GI number: 153952198

Start: 1483

End: 2550

Strand: Direct

Name: dnaN [H]

Synonym: JJD26997_0002

Alternate gene names: 153952198

Gene position: 1483-2550 (Clockwise)

Preceding gene: 153951168

Following gene: 153951349

Centisome position: 0.08

GC content: 25.56

Gene sequence:

>1068_bases
ATGAAGTTAAGTATCAATAAAAATACTTTAGAATCTGCAGTGATTTTATGCAATGCTTATGTAGAAAAAAAAGACTCAAG
TACTATTACTTCTCATCTTTTTTTTCATGCTGATGAAGATAAACTTCTTATTAGAGCTAGTGATTATGAAATAGGTATCA
ACTATAAGATAAAAAAAATCCGTGTAGAATCAAGTGGTTTTGCTACTGCAAATGCAAAAAGTATTACAGATGTTATTAAA
AACTTAAACAACGAAGAAATTGTTTTAGAAACCATTGATAATTTCTTATTTATAAGACAAAAAAGCACAAAATACAAACT
TCCTATGTTTAATCATGAAGATTTTCCAAATTTTCCAAATACAGAAGGAAAAAATCAATTTGACATTGATTCAAGTGATT
TAAGTCGTTCTCTTAAAAAGATATTACCAAGTATTGATACAAACAACCCAAAATACTCCTTAAATGGTGCATTTTTAGAT
ATAAAAACAGATAAAATTAACTTCGTAGGAACTGATACAAAACGCCTTGCAATTTATACTCTAGAAAAAGCAAATAATCA
AGAATTTAGTTTTAGTATCCCTAAAAAAGCTATTATGGAAATGCAAAAACTTTTCTATGAAAAAATAGAAATTTTTTATG
ATCAAAATATGCTTATTGCTAAAAATGAAAATTTTGAATTCTTTACAAAACTTATCAATGATAAATTTCCAGATTATGAA
AAAGTTATACCAAAAACTTTCAAGCAAGAACTCAGCTTTTCAACTGAAGATTTTATAGATAGTCTTAAAAAAATAAGCGT
TGTAACTGAAAAAATGAAACTTCATTTTAACAAAAATAAAATCATCTTTGAAGGTATAAGTTTAGATAATATGGAAGCAA
AAACAGAGCTTGAAATTCAAACAGGAGTAAGTGAAGAATTTAATCTTACTATAAAAATTAAACATTTACTCGATTTCTTA
ACTTCTATAGAAGAAGAAAAATTCACTTTAAGTGTAAGTGAACCTAATTCAGCATTTATAGTCAAATCTCAGGGACTATC
AATGATTATCATGCCTATGATTTTATAA

Upstream 100 bases:

>100_bases
ATAAAATACAAATTTGATTATTTTGCTTTGAAGAATTTCACAATTTCAACAAGCTTATTATTACAACGAATTTAAAATTA
AAATAAACCAAGGAGAAAAA

Downstream 100 bases:

>100_bases
CAAAAACAAGTAAAAATAAAGGAAAAATATGCAAGAAAATTACGGTGCGAGTAATATTAAAGTCCTAAAAGGCTTAGAAG
CTGTTAGAAAACGCCCAGGT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKLSINKNTLESAVILCNAYVEKKDSSTITSHLFFHADEDKLLIRASDYEIGINYKIKKIRVESSGFATANAKSITDVIK
NLNNEEIVLETIDNFLFIRQKSTKYKLPMFNHEDFPNFPNTEGKNQFDIDSSDLSRSLKKILPSIDTNNPKYSLNGAFLD
IKTDKINFVGTDTKRLAIYTLEKANNQEFSFSIPKKAIMEMQKLFYEKIEIFYDQNMLIAKNENFEFFTKLINDKFPDYE
KVIPKTFKQELSFSTEDFIDSLKKISVVTEKMKLHFNKNKIIFEGISLDNMEAKTELEIQTGVSEEFNLTIKIKHLLDFL
TSIEEEKFTLSVSEPNSAFIVKSQGLSMIIMPMIL

Sequences:

>Translated_355_residues
MKLSINKNTLESAVILCNAYVEKKDSSTITSHLFFHADEDKLLIRASDYEIGINYKIKKIRVESSGFATANAKSITDVIK
NLNNEEIVLETIDNFLFIRQKSTKYKLPMFNHEDFPNFPNTEGKNQFDIDSSDLSRSLKKILPSIDTNNPKYSLNGAFLD
IKTDKINFVGTDTKRLAIYTLEKANNQEFSFSIPKKAIMEMQKLFYEKIEIFYDQNMLIAKNENFEFFTKLINDKFPDYE
KVIPKTFKQELSFSTEDFIDSLKKISVVTEKMKLHFNKNKIIFEGISLDNMEAKTELEIQTGVSEEFNLTIKIKHLLDFL
TSIEEEKFTLSVSEPNSAFIVKSQGLSMIIMPMIL
>Mature_355_residues
MKLSINKNTLESAVILCNAYVEKKDSSTITSHLFFHADEDKLLIRASDYEIGINYKIKKIRVESSGFATANAKSITDVIK
NLNNEEIVLETIDNFLFIRQKSTKYKLPMFNHEDFPNFPNTEGKNQFDIDSSDLSRSLKKILPSIDTNNPKYSLNGAFLD
IKTDKINFVGTDTKRLAIYTLEKANNQEFSFSIPKKAIMEMQKLFYEKIEIFYDQNMLIAKNENFEFFTKLINDKFPDYE
KVIPKTFKQELSFSTEDFIDSLKKISVVTEKMKLHFNKNKIIFEGISLDNMEAKTELEIQTGVSEEFNLTIKIKHLLDFL
TSIEEEKFTLSVSEPNSAFIVKSQGLSMIIMPMIL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=367, Percent_Identity=19.0735694822888, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40996; Mature: 40996

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSINKNTLESAVILCNAYVEKKDSSTITSHLFFHADEDKLLIRASDYEIGINYKIKKI
CEEECCCCHHHHHHEEEEHHHCCCCCCCEEEEEEEEECCCEEEEEECCEEEECEEEEEEE
RVESSGFATANAKSITDVIKNLNNEEIVLETIDNFLFIRQKSTKYKLPMFNHEDFPNFPN
EEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCEEECCCCCCCCCCCCCC
TEGKNQFDIDSSDLSRSLKKILPSIDTNNPKYSLNGAFLDIKTDKINFVGTDTKRLAIYT
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECEEEEEEEECEEEEEECCCCEEEEEE
LEKANNQEFSFSIPKKAIMEMQKLFYEKIEIFYDQNMLIAKNENFEFFTKLINDKFPDYE
EECCCCCEEEEECCHHHHHHHHHHHHHHHHEEECCCEEEEECCCHHHHHHHHCCCCCCHH
KVIPKTFKQELSFSTEDFIDSLKKISVVTEKMKLHFNKNKIIFEGISLDNMEAKTELEIQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCCEEEEEE
TGVSEEFNLTIKIKHLLDFLTSIEEEKFTLSVSEPNSAFIVKSQGLSMIIMPMIL
ECCCCCEEEEEEHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCEEEEECCC
>Mature Secondary Structure
MKLSINKNTLESAVILCNAYVEKKDSSTITSHLFFHADEDKLLIRASDYEIGINYKIKKI
CEEECCCCHHHHHHEEEEHHHCCCCCCCEEEEEEEEECCCEEEEEECCEEEECEEEEEEE
RVESSGFATANAKSITDVIKNLNNEEIVLETIDNFLFIRQKSTKYKLPMFNHEDFPNFPN
EEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCEEECCCCCCCCCCCCCC
TEGKNQFDIDSSDLSRSLKKILPSIDTNNPKYSLNGAFLDIKTDKINFVGTDTKRLAIYT
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECEEEEEEEECEEEEEECCCCEEEEEE
LEKANNQEFSFSIPKKAIMEMQKLFYEKIEIFYDQNMLIAKNENFEFFTKLINDKFPDYE
EECCCCCEEEEECCHHHHHHHHHHHHHHHHEEECCCEEEEECCCHHHHHHHHCCCCCCHH
KVIPKTFKQELSFSTEDFIDSLKKISVVTEKMKLHFNKNKIIFEGISLDNMEAKTELEIQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCCEEEEEE
TGVSEEFNLTIKIKHLLDFLTSIEEEKFTLSVSEPNSAFIVKSQGLSMIIMPMIL
ECCCCCEEEEEEHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]