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The map label for this gene is hisF-2 [H]

Identifier: 153952109

GI number: 153952109

Start: 1727586

End: 1728353

Strand: Direct

Name: hisF-2 [H]

Synonym: JJD26997_1957

Alternate gene names: 153952109

Gene position: 1727586-1728353 (Clockwise)

Preceding gene: 153951118

Following gene: 153951441

Centisome position: 93.63

GC content: 33.07

Gene sequence:

>768_bases
ATGCTTACAAAACGCATAATTGCATGTTTAGATGTCAAAGATGGCAGAGTAGTAAAAGGAACACAATTTAAAAACCATAA
AGATATGGGAGATATCATAGAACTTGCTAGGTATTATTCTCAAAATGGTATTGATGAGCTTGTTTTTTATGATATTGCTG
CAAGTGCTAGAAAAGAACGCATATCAAGAGAATGGGTAAGTGAAGTAGCAAAAAATATCAATATTCCTTTTTGTGTTGCA
GGGGGTATTAAAAGCGAAGAAGATGCAGCTGAACTTTTAGCTAATGGGGCAGATAAAATTTCTATCAATTCTCCTGCTTT
AAATGATCCAAGCCTAATCACACGCCTTGCTAAAAGCTTTGGCGTGCAATGCGTAGTAGTAGGAATTGATAGTTTCAAAG
ATGAAAATGGAAATTTAAAAGTATTTCAATACACAGGCGATGAAAAAACAAGCAAACATAGTGGAAAAAGCACTTTAGAA
TGGGTTAAAGAAGTGCAAGATTTAGGTGCAGGCGAAATTGTTTTAAATATGATGAATCAAGATGGCGTTAAAAATGGCTA
TGATTTAGAACAATTAGAAGCTGTATATAAAATTTGTAAAATACCTTTGATCGCAAGCGGGGGAGCGGGAAAAATGGAGC
ATTTTTTAGAAGCTTTTAAACTAGGTATAGATGGGGCTTTAGCTGCTTCTGTTTTTCATCAAAAACTTATAGACATTAAA
GAATTAAAAATTTATCTTAAAAATCAAGGCTTAAGTATAAGGATATAA

Upstream 100 bases:

>100_bases
CGAGCTTAAAAGACCTTGAAAACTTAAAAGGAATTTGTAGTGGGGTTATCGTAGGAAAAGCTTTATTAGACGGAGTTTTT
AGTGTAGAAGAAGGGATAAG

Downstream 100 bases:

>100_bases
TTATGCAAAATTTCAAAGAACTTAATGAAAAAATCGCTTGGCAAAAAGTGGATCATCTTTTACCCGTCATCGTCCAAGAT
GCAAAAACTTGTGAAGTTTT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF1; ImGP synthase subunit hisF1; IGPS subunit hisF1 [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLTKRIIACLDVKDGRVVKGTQFKNHKDMGDIIELARYYSQNGIDELVFYDIAASARKERISREWVSEVAKNINIPFCVA
GGIKSEEDAAELLANGADKISINSPALNDPSLITRLAKSFGVQCVVVGIDSFKDENGNLKVFQYTGDEKTSKHSGKSTLE
WVKEVQDLGAGEIVLNMMNQDGVKNGYDLEQLEAVYKICKIPLIASGGAGKMEHFLEAFKLGIDGALAASVFHQKLIDIK
ELKIYLKNQGLSIRI

Sequences:

>Translated_255_residues
MLTKRIIACLDVKDGRVVKGTQFKNHKDMGDIIELARYYSQNGIDELVFYDIAASARKERISREWVSEVAKNINIPFCVA
GGIKSEEDAAELLANGADKISINSPALNDPSLITRLAKSFGVQCVVVGIDSFKDENGNLKVFQYTGDEKTSKHSGKSTLE
WVKEVQDLGAGEIVLNMMNQDGVKNGYDLEQLEAVYKICKIPLIASGGAGKMEHFLEAFKLGIDGALAASVFHQKLIDIK
ELKIYLKNQGLSIRI
>Mature_255_residues
MLTKRIIACLDVKDGRVVKGTQFKNHKDMGDIIELARYYSQNGIDELVFYDIAASARKERISREWVSEVAKNINIPFCVA
GGIKSEEDAAELLANGADKISINSPALNDPSLITRLAKSFGVQCVVVGIDSFKDENGNLKVFQYTGDEKTSKHSGKSTLE
WVKEVQDLGAGEIVLNMMNQDGVKNGYDLEQLEAVYKICKIPLIASGGAGKMEHFLEAFKLGIDGALAASVFHQKLIDIK
ELKIYLKNQGLSIRI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=66.147859922179, Blast_Score=348, Evalue=1e-97,
Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=24.5833333333333, Blast_Score=75, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=33.4394904458599, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 28085; Mature: 28085

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIACLDVKDGRVVKGTQFKNHKDMGDIIELARYYSQNGIDELVFYDIAASARKER
CCHHHEEHEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
ISREWVSEVAKNINIPFCVAGGIKSEEDAAELLANGADKISINSPALNDPSLITRLAKSF
HHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
GVQCVVVGIDSFKDENGNLKVFQYTGDEKTSKHSGKSTLEWVKEVQDLGAGEIVLNMMNQ
CCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHC
DGVKNGYDLEQLEAVYKICKIPLIASGGAGKMEHFLEAFKLGIDGALAASVFHQKLIDIK
CCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ELKIYLKNQGLSIRI
HHHHEEECCCCEEEC
>Mature Secondary Structure
MLTKRIIACLDVKDGRVVKGTQFKNHKDMGDIIELARYYSQNGIDELVFYDIAASARKER
CCHHHEEHEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
ISREWVSEVAKNINIPFCVAGGIKSEEDAAELLANGADKISINSPALNDPSLITRLAKSF
HHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
GVQCVVVGIDSFKDENGNLKVFQYTGDEKTSKHSGKSTLEWVKEVQDLGAGEIVLNMMNQ
CCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHC
DGVKNGYDLEQLEAVYKICKIPLIASGGAGKMEHFLEAFKLGIDGALAASVFHQKLIDIK
CCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ELKIYLKNQGLSIRI
HHHHEEECCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]