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The map label for this gene is mboIBM [H]

Identifier: 153951969

GI number: 153951969

Start: 1725441

End: 1726289

Strand: Direct

Name: mboIBM [H]

Synonym: JJD26997_1954

Alternate gene names: 153951969

Gene position: 1725441-1726289 (Clockwise)

Preceding gene: 153951253

Following gene: 153951018

Centisome position: 93.51

GC content: 27.44

Gene sequence:

>849_bases
ATGATAACTGGTTTGAAACCTATTTTCACTAGCAAAGATGAAAATTTTATCCTTTATCAAAATGATTGTAATAAACTATT
GCCTAAATTTGAAAAACAATTTGATTTAATCTTTGCCGATCCACCATATTTTCTTTCAAACGATGGTTTAAGTATTCAAA
ACGGTAAAATTGTTAGTGTAAACAAAGGAGATTGGGACAAAGGTAATGATATTGAAAAAATAGACGAGTTTAATTTAAAA
TGGTTATCTAATGCAAAAATAGCACTTCAAGATACTGGAAGTATTTTGATTTGTGGGACTTATCATAATATTTTTTCACT
TGGTAGAATTTTACAAAAGCTTGATTTTAAAATTTTAAATATTATTACTTGGCAAAAAACAAATCCTCCGCCAAATTTTA
GCTGTCGTTATCTTACACATTCGACAGAACAAATTATTTGGGCTAGAAAAAGTCATAAATATAAGCATATTTTTAATTAT
GAAATTTTAAGATTTTTAAATAGCAACAAACAAATGCGTGATGTTTGGACTTTCAATGCTATTGCTCCTTGGGAAAAAAC
AAATGGAAAACATCCTACACAAAAACCTTTAGCTTTGCTTACCAGACTTATTTTAATGGCAAGCAATGAAGATTGTTTGA
TTTGCGATCCTTTTAGTGGAAGCTCAACCACAGGTATTGGAGCAAATTTATTAAATAGAAAATTCATAGGTTTTGAGAAA
GAAAGTGAATTTATAGAAATATCAATAGCTAGAAAGAAAGAATTAGAAAATAATTTTAACACCATTAAAAGCAAAATTAA
TGATTTGAAATTATTGGGTAAAAATAGTTTATTTCAAGGAGTGATATGA

Upstream 100 bases:

>100_bases
TGTATTGTATTAAGTGGCTATTCATAGAACAAGATGTTACTTATTGGAATTATTCAGGAAGAAGGAAATTTTTTGAAGCT
CTTAATGAATGTGAGTTAGT

Downstream 100 bases:

>100_bases
AACTAGTTATCATCGATACAGCCTGTGCGAATTTGGCATCTTTGAAATTTTGTCTGGATAGATTAGGTTTTAATGCAACT
ATCAGCAGGGATTTAAAAGA

Product: DNA methylase

Products: NA

Alternate protein names: M.MboIB; Adenine-specific methyltransferase MboIB; M.MboI C [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MITGLKPIFTSKDENFILYQNDCNKLLPKFEKQFDLIFADPPYFLSNDGLSIQNGKIVSVNKGDWDKGNDIEKIDEFNLK
WLSNAKIALQDTGSILICGTYHNIFSLGRILQKLDFKILNIITWQKTNPPPNFSCRYLTHSTEQIIWARKSHKYKHIFNY
EILRFLNSNKQMRDVWTFNAIAPWEKTNGKHPTQKPLALLTRLILMASNEDCLICDPFSGSSTTGIGANLLNRKFIGFEK
ESEFIEISIARKKELENNFNTIKSKINDLKLLGKNSLFQGVI

Sequences:

>Translated_282_residues
MITGLKPIFTSKDENFILYQNDCNKLLPKFEKQFDLIFADPPYFLSNDGLSIQNGKIVSVNKGDWDKGNDIEKIDEFNLK
WLSNAKIALQDTGSILICGTYHNIFSLGRILQKLDFKILNIITWQKTNPPPNFSCRYLTHSTEQIIWARKSHKYKHIFNY
EILRFLNSNKQMRDVWTFNAIAPWEKTNGKHPTQKPLALLTRLILMASNEDCLICDPFSGSSTTGIGANLLNRKFIGFEK
ESEFIEISIARKKELENNFNTIKSKINDLKLLGKNSLFQGVI
>Mature_282_residues
MITGLKPIFTSKDENFILYQNDCNKLLPKFEKQFDLIFADPPYFLSNDGLSIQNGKIVSVNKGDWDKGNDIEKIDEFNLK
WLSNAKIALQDTGSILICGTYHNIFSLGRILQKLDFKILNIITWQKTNPPPNFSCRYLTHSTEQIIWARKSHKYKHIFNY
EILRFLNSNKQMRDVWTFNAIAPWEKTNGKHPTQKPLALLTRLILMASNEDCLICDPFSGSSTTGIGANLLNRKFIGFEK
ESEFIEISIARKKELENNFNTIKSKINDLKLLGKNSLFQGVI

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MboI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=275, Percent_Identity=27.6363636363636, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32473; Mature: 32473

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITGLKPIFTSKDENFILYQNDCNKLLPKFEKQFDLIFADPPYFLSNDGLSIQNGKIVSV
CCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCEEEEECCCCCEECCCCCEECCCEEEEE
NKGDWDKGNDIEKIDEFNLKWLSNAKIALQDTGSILICGTYHNIFSLGRILQKLDFKILN
CCCCCCCCCCHHHHHCCCEEEECCCEEEEECCCCEEEEECHHHHHHHHHHHHHHCCEEEE
IITWQKTNPPPNFSCRYLTHSTEQIIWARKSHKYKHIFNYEILRFLNSNKQMRDVWTFNA
EEEECCCCCCCCCEEEEEECCCHHEEEECCCCCEEEHHHHHHHHHHCCCCCEEEEEEECC
IAPWEKTNGKHPTQKPLALLTRLILMASNEDCLICDPFSGSSTTGIGANLLNRKFIGFEK
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHCCCCCCCCC
ESEFIEISIARKKELENNFNTIKSKINDLKLLGKNSLFQGVI
CCCEEEEEEHHHHHHHHHHHHHHHHHHHHEEECCCCHHCCCC
>Mature Secondary Structure
MITGLKPIFTSKDENFILYQNDCNKLLPKFEKQFDLIFADPPYFLSNDGLSIQNGKIVSV
CCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCEEEEECCCCCEECCCCCEECCCEEEEE
NKGDWDKGNDIEKIDEFNLKWLSNAKIALQDTGSILICGTYHNIFSLGRILQKLDFKILN
CCCCCCCCCCHHHHHCCCEEEECCCEEEEECCCCEEEEECHHHHHHHHHHHHHHCCEEEE
IITWQKTNPPPNFSCRYLTHSTEQIIWARKSHKYKHIFNYEILRFLNSNKQMRDVWTFNA
EEEECCCCCCCCCEEEEEECCCHHEEEECCCCCEEEHHHHHHHHHHCCCCCEEEEEEECC
IAPWEKTNGKHPTQKPLALLTRLILMASNEDCLICDPFSGSSTTGIGANLLNRKFIGFEK
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHCCCCCCCCC
ESEFIEISIARKKELENNFNTIKSKINDLKLLGKNSLFQGVI
CCCEEEEEEHHHHHHHHHHHHHHHHHHHHEEECCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8506128 [H]