Click here to switch to the map view.

The map label for this gene is radA [H]

Identifier: 153951878

GI number: 153951878

Start: 470792

End: 472132

Strand: Direct

Name: radA [H]

Synonym: JJD26997_0525

Alternate gene names: 153951878

Gene position: 470792-472132 (Clockwise)

Preceding gene: 153951245

Following gene: 153952326

Centisome position: 25.52

GC content: 35.27

Gene sequence:

>1341_bases
ATGGCTAAAAAACACATTTTATTTGAATGTCAAACTTGTGGAAATCAACAAAGCAAATGGCTTGGAAAATGTCCTGATTG
TGGAGCTTGGGATAGTTTTGTAGAATTAAAAGCTGAGCAAATTAAAGTTTTAAAAGAGCTTGCACAAGTTAACATGAAAA
CAAGTGAAGCTGTCTGTATTGAAGATGTGGAATTGGAGTATTTTACAAGATACAGCACAGATGATAATGAGCTTGATTTG
GTTTTAGGTGGAGGGCTTGTTGAAGGTTCTTTAGTGCTTATAGGTGGAAGTCCAGGTGTGGGAAAATCCACACTTTTACT
AAAAATTGCTTCAAATTTAGCCAAACAGGATAAAAAAGTGCTTTATGTAAGTGGTGAAGAAAGTAAAGCTCAGATTAAGT
TAAGAGCTGATCGCCTTGAGGCTAATACTCCAAATTTATTTTTACTTACTGAACTTTGCCTTGAAAATATTTTAGAAGAA
TTGCACAAAAAAGATTATAGCATTCTTATTGTTGACTCTATACAAACTTTATATTCAAATAAAATTACTTCGGCAGCAGG
AAGTATCACTCAAGTGCGTGAGATTACTTTTGAGCTTATGCGTGTTAGCAAGGCTTATAATATTAGCACTTTTATCATAG
GGCATATTACCAAAGAAGGTGCTATAGCAGGACCTAGAGTTCTTGAGCATATGGTAGATGTGGTGCTTTATTTTGAAGGA
GATGCCACTAAAGAAATCAGACTTTTAAGAGGTTTTAAAAATCGTTTTGGTGGAACGAATGAAGTAGGTATTTTTGAGAT
GACCTCTAAGGGTTTGATCAGCGCAAAAGATTTGGCAAATCGTTTTTTTACTCGTGGAAAGGCTATTTCAGGAAGCGCTT
TAGGGGTTGTGATGGAAGGATCTCGTGCTTTGGTTTTAGAAGTTCAAGCCTTAGTATGTGAAAGTTCTTATCCAAAACGC
AGTGCTACGGGCTATGAAAAAAATCGCTTAGATATGCTTTTGGCCTTACTTGAAAGAAAGCTTGAAATTCCTTTAGGGCA
TTATGATGTATTTGTAAATATTAGTGGCGGGGTAAAAGTAAGTGAGACTGCGGCAGATTTGGCTGTGGTTGCTGCGATTA
TTTCAAGTTTTAAAAATCGCCCTTTAAGTAAAGATAGTATTTTTATAGGAGAGCTTAGTTTAAATGGAGAAATTAGAGAG
GTTTTTAGCCTTGATACGCGTTTAAAAGAAGCTAAAATGCAAAAATTTAAAAATGCTATTGTTCCTTCTAAGCCTTCGGA
AGATATAGGGCTTAAATGCTTTGTAGCTAAAGAACTTTCGCAAGTTTTAGAATGGATGTAA

Upstream 100 bases:

>100_bases
GAGGAAATTCAAAATCATTTATTACCCTTACGAAAAAGTTTGCAAAAAGAAAATTAAAAGTTAATTCTTAATATAATTTA
TAAAAATTAAGGTAACCTAA

Downstream 100 bases:

>100_bases
ATTTTCATTAAAATATAAGTAAGATTTTGCTAGACTTTCGAGTTTAGAATAAAATTTTTTGAGGAAACCATGAAAGATTT
ATTTTTATTTAGCTCTTTGC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MAKKHILFECQTCGNQQSKWLGKCPDCGAWDSFVELKAEQIKVLKELAQVNMKTSEAVCIEDVELEYFTRYSTDDNELDL
VLGGGLVEGSLVLIGGSPGVGKSTLLLKIASNLAKQDKKVLYVSGEESKAQIKLRADRLEANTPNLFLLTELCLENILEE
LHKKDYSILIVDSIQTLYSNKITSAAGSITQVREITFELMRVSKAYNISTFIIGHITKEGAIAGPRVLEHMVDVVLYFEG
DATKEIRLLRGFKNRFGGTNEVGIFEMTSKGLISAKDLANRFFTRGKAISGSALGVVMEGSRALVLEVQALVCESSYPKR
SATGYEKNRLDMLLALLERKLEIPLGHYDVFVNISGGVKVSETAADLAVVAAIISSFKNRPLSKDSIFIGELSLNGEIRE
VFSLDTRLKEAKMQKFKNAIVPSKPSEDIGLKCFVAKELSQVLEWM

Sequences:

>Translated_446_residues
MAKKHILFECQTCGNQQSKWLGKCPDCGAWDSFVELKAEQIKVLKELAQVNMKTSEAVCIEDVELEYFTRYSTDDNELDL
VLGGGLVEGSLVLIGGSPGVGKSTLLLKIASNLAKQDKKVLYVSGEESKAQIKLRADRLEANTPNLFLLTELCLENILEE
LHKKDYSILIVDSIQTLYSNKITSAAGSITQVREITFELMRVSKAYNISTFIIGHITKEGAIAGPRVLEHMVDVVLYFEG
DATKEIRLLRGFKNRFGGTNEVGIFEMTSKGLISAKDLANRFFTRGKAISGSALGVVMEGSRALVLEVQALVCESSYPKR
SATGYEKNRLDMLLALLERKLEIPLGHYDVFVNISGGVKVSETAADLAVVAAIISSFKNRPLSKDSIFIGELSLNGEIRE
VFSLDTRLKEAKMQKFKNAIVPSKPSEDIGLKCFVAKELSQVLEWM
>Mature_445_residues
AKKHILFECQTCGNQQSKWLGKCPDCGAWDSFVELKAEQIKVLKELAQVNMKTSEAVCIEDVELEYFTRYSTDDNELDLV
LGGGLVEGSLVLIGGSPGVGKSTLLLKIASNLAKQDKKVLYVSGEESKAQIKLRADRLEANTPNLFLLTELCLENILEEL
HKKDYSILIVDSIQTLYSNKITSAAGSITQVREITFELMRVSKAYNISTFIIGHITKEGAIAGPRVLEHMVDVVLYFEGD
ATKEIRLLRGFKNRFGGTNEVGIFEMTSKGLISAKDLANRFFTRGKAISGSALGVVMEGSRALVLEVQALVCESSYPKRS
ATGYEKNRLDMLLALLERKLEIPLGHYDVFVNISGGVKVSETAADLAVVAAIISSFKNRPLSKDSIFIGELSLNGEIREV
FSLDTRLKEAKMQKFKNAIVPSKPSEDIGLKCFVAKELSQVLEWM

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=449, Percent_Identity=42.7616926503341, Blast_Score=353, Evalue=2e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49222; Mature: 49090

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKHILFECQTCGNQQSKWLGKCPDCGAWDSFVELKAEQIKVLKELAQVNMKTSEAVCI
CCCCEEEEEECCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
EDVELEYFTRYSTDDNELDLVLGGGLVEGSLVLIGGSPGVGKSTLLLKIASNLAKQDKKV
EECCEEEEEECCCCCCCEEEEEECCEEECCEEEEECCCCCCCHHHHHHHHHHHHHCCCEE
LYVSGEESKAQIKLRADRLEANTPNLFLLTELCLENILEELHKKDYSILIVDSIQTLYSN
EEEECCCCCEEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHC
KITSAAGSITQVREITFELMRVSKAYNISTFIIGHITKEGAIAGPRVLEHMVDVVLYFEG
CHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHEEEEECC
DATKEIRLLRGFKNRFGGTNEVGIFEMTSKGLISAKDLANRFFTRGKAISGSALGVVMEG
CCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEC
SRALVLEVQALVCESSYPKRSATGYEKNRLDMLLALLERKLEIPLGHYDVFVNISGGVKV
CCEEEEEEHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEE
SETAADLAVVAAIISSFKNRPLSKDSIFIGELSLNGEIREVFSLDTRLKEAKMQKFKNAI
CHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
VPSKPSEDIGLKCFVAKELSQVLEWM
CCCCCCCCCCEEEEHHHHHHHHHHCC
>Mature Secondary Structure 
AKKHILFECQTCGNQQSKWLGKCPDCGAWDSFVELKAEQIKVLKELAQVNMKTSEAVCI
CCCEEEEEECCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
EDVELEYFTRYSTDDNELDLVLGGGLVEGSLVLIGGSPGVGKSTLLLKIASNLAKQDKKV
EECCEEEEEECCCCCCCEEEEEECCEEECCEEEEECCCCCCCHHHHHHHHHHHHHCCCEE
LYVSGEESKAQIKLRADRLEANTPNLFLLTELCLENILEELHKKDYSILIVDSIQTLYSN
EEEECCCCCEEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHC
KITSAAGSITQVREITFELMRVSKAYNISTFIIGHITKEGAIAGPRVLEHMVDVVLYFEG
CHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHEEEEECC
DATKEIRLLRGFKNRFGGTNEVGIFEMTSKGLISAKDLANRFFTRGKAISGSALGVVMEG
CCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEC
SRALVLEVQALVCESSYPKRSATGYEKNRLDMLLALLERKLEIPLGHYDVFVNISGGVKV
CCEEEEEEHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEE
SETAADLAVVAAIISSFKNRPLSKDSIFIGELSLNGEIREVFSLDTRLKEAKMQKFKNAI
CHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
VPSKPSEDIGLKCFVAKELSQVLEWM
CCCCCCCCCCEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]