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The map label for this gene is gidA [H]

Identifier: 153951262

GI number: 153951262

Start: 488780

End: 490639

Strand: Direct

Name: gidA [H]

Synonym: JJD26997_0541

Alternate gene names: 153951262

Gene position: 488780-490639 (Clockwise)

Preceding gene: 153951873

Following gene: 153952394

Centisome position: 26.49

GC content: 33.01

Gene sequence:

>1860_bases
ATGTTTGATGTCATTGTTATAGGTGGTGGACATGCAGGTGTTGAAGCAAGTGCTGCTGCTGCTAGAATGGGTAAAAAAAC
CTTACTTTTAACTACATTGATCGAGCAAATAGGAGCAGCAAGTTGCAATCCAGCTATAGGTGGACTTGCTAAAGGGCATT
TGGTAAAAGAACTTGATGCCATGGGTGGTTTTATGGGTGAAATCACCGATGAAGCAGGGATACAATTTCGTATTTTAAAT
GAAAGTAAAGGTGTAGCTGTTCAAGGAAGTAGGGTGCAAATTGATATGGATAAATATCGTATCATTGCGCGCAATAAACT
TTTAAAACTTCCAAATTTAGAAATCTCTCAAGAACAAGTGAGTGCTTTAATCGTAGAAAATGATAAAGTTAAAGGCGTAA
AAACAAATTTAGAAAATATATATTTTGCCAAAAAAGTGATACTTACAACAGGAACTTTTTTAAATGGACTCATTCATGTA
GGTGAAAATAAACTTCAAGCAGGAAGAGTAGGGGAGCTTGCTTCAGTAAATTTAGGAAACTATTTACAGACTTTGGGTTT
AAAAATGGGACGTTTAAAAACAGGAACTTGTCCGAGAGTGGATGCAACAAGCATTGATTTTAGCGTACTTGAAATTCAAG
ATGGCGATGTAAATCCTAAAGCTTTTAGCTTTCGTTCAAGAAATTTTAATCCTACTCAACTTCCTTGTTATATCGCACGT
ACTAATACAACTACACATGAGATTATTAAGAATAATTTTTATCGTGCCCCACTTTTTACAGGTCAGATTGAAGGTGTGGG
GCCAAGGTATTGTCCTTCTATAGAAGATAAGATCAATCGTTTTAGCGACAAAGAAAGCCATCATCTTTTTATAGAGCCTC
AAACTATCGATGCGACGGAGTATTATATCAATGGTTTTTCTACTTCTTTACCTTATGAAGTGCAAACTCAAATGCTGCAT
TCTGTAAAAGGTTTTGAAAATGCTAAAATAACCCGTTTTGGTTATGCTATAGAATATGATTATATAGAGCCAACTGAACT
TAAGCATACTTTAGAACTTAAAAAGATTAAAAATCTTTATTGTGCAGGACAGATTAATGGAACTACGGGTTATGAAGAGG
CTGCAGTACAAGGCTTTATGGCAGGAATCAATGCAAGTTTAAGCATAGATATGAAAGAACCTTTGATTTTAAGGCGTGAT
GAAGCTTATATAGGAGTTTTAATCGATGATTTAGTAGTAAAAGGCACAAAAGAACCTTATAGAATGTTTACTTCAAGGGC
AGAATTTAGACTACTTTTAAGGGAAGAAAATGCTATACTTAGACTTGGAAAATATGGCTATGTTTTAGGGCTTTTAAGCG
AGCAAGATTTTGCTTATATACAAAATATTGCAAACAATCTTCAAAAAGGACTTGAATTTTTACTTTCTAAAGAATTTACA
CCTAATAACCAAAATAATGCTTTTTTAGAGAGTTTAGGAGAGGATAAAATTTCTTCTATAGTCAATTTTCAAAAAATTGT
TGCAAGAGCAAGTTTTGATATAGAAAAATTAAAAAAACTTGATCCTATGTTTGAAACCATGGATAATTACTCTTTAAGAG
AGATTTTAAACGAGGCAAAGTATTGTCATTACATCAGTATGCAAAAAGCTCAAGTAGAAAAAATGAAAAATCTTAGTGAG
CTTAAAATTCCTGAAAATTTTGATTTTAAAAGTGTAAGCGGTTTGAGTAATGAGGTTGTAGAAAAACTTAATCATCATAA
ACCTCCAACCATTTTTGCTGCAAGTCAAATCAGTGGAATCACTCCTGCGGCGTTAGATATTTTACAAATTTATATCAAAA
TGCAAAAGAAAAAAGCTTAA

Upstream 100 bases:

>100_bases
AGAAGCTAAATGAATTTTTGTTAGACTTAAAACGAGTTTGTTTTAAGTCTAAATATTATATAATCTTTCTTTTTAAAATT
CACATTTCTCAAGGCAAAAT

Downstream 100 bases:

>100_bases
ATGCTTGCTTTTTTTATTTTTTTGTTTACTTTAATTTTACTTTTTTGGCGTCCATGGAATTTACCTATTTGGGTATTTTC
TAGCTTGGGAGCTTTTTTTG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MFDVIVIGGGHAGVEASAAAARMGKKTLLLTTLIEQIGAASCNPAIGGLAKGHLVKELDAMGGFMGEITDEAGIQFRILN
ESKGVAVQGSRVQIDMDKYRIIARNKLLKLPNLEISQEQVSALIVENDKVKGVKTNLENIYFAKKVILTTGTFLNGLIHV
GENKLQAGRVGELASVNLGNYLQTLGLKMGRLKTGTCPRVDATSIDFSVLEIQDGDVNPKAFSFRSRNFNPTQLPCYIAR
TNTTTHEIIKNNFYRAPLFTGQIEGVGPRYCPSIEDKINRFSDKESHHLFIEPQTIDATEYYINGFSTSLPYEVQTQMLH
SVKGFENAKITRFGYAIEYDYIEPTELKHTLELKKIKNLYCAGQINGTTGYEEAAVQGFMAGINASLSIDMKEPLILRRD
EAYIGVLIDDLVVKGTKEPYRMFTSRAEFRLLLREENAILRLGKYGYVLGLLSEQDFAYIQNIANNLQKGLEFLLSKEFT
PNNQNNAFLESLGEDKISSIVNFQKIVARASFDIEKLKKLDPMFETMDNYSLREILNEAKYCHYISMQKAQVEKMKNLSE
LKIPENFDFKSVSGLSNEVVEKLNHHKPPTIFAASQISGITPAALDILQIYIKMQKKKA

Sequences:

>Translated_619_residues
MFDVIVIGGGHAGVEASAAAARMGKKTLLLTTLIEQIGAASCNPAIGGLAKGHLVKELDAMGGFMGEITDEAGIQFRILN
ESKGVAVQGSRVQIDMDKYRIIARNKLLKLPNLEISQEQVSALIVENDKVKGVKTNLENIYFAKKVILTTGTFLNGLIHV
GENKLQAGRVGELASVNLGNYLQTLGLKMGRLKTGTCPRVDATSIDFSVLEIQDGDVNPKAFSFRSRNFNPTQLPCYIAR
TNTTTHEIIKNNFYRAPLFTGQIEGVGPRYCPSIEDKINRFSDKESHHLFIEPQTIDATEYYINGFSTSLPYEVQTQMLH
SVKGFENAKITRFGYAIEYDYIEPTELKHTLELKKIKNLYCAGQINGTTGYEEAAVQGFMAGINASLSIDMKEPLILRRD
EAYIGVLIDDLVVKGTKEPYRMFTSRAEFRLLLREENAILRLGKYGYVLGLLSEQDFAYIQNIANNLQKGLEFLLSKEFT
PNNQNNAFLESLGEDKISSIVNFQKIVARASFDIEKLKKLDPMFETMDNYSLREILNEAKYCHYISMQKAQVEKMKNLSE
LKIPENFDFKSVSGLSNEVVEKLNHHKPPTIFAASQISGITPAALDILQIYIKMQKKKA
>Mature_619_residues
MFDVIVIGGGHAGVEASAAAARMGKKTLLLTTLIEQIGAASCNPAIGGLAKGHLVKELDAMGGFMGEITDEAGIQFRILN
ESKGVAVQGSRVQIDMDKYRIIARNKLLKLPNLEISQEQVSALIVENDKVKGVKTNLENIYFAKKVILTTGTFLNGLIHV
GENKLQAGRVGELASVNLGNYLQTLGLKMGRLKTGTCPRVDATSIDFSVLEIQDGDVNPKAFSFRSRNFNPTQLPCYIAR
TNTTTHEIIKNNFYRAPLFTGQIEGVGPRYCPSIEDKINRFSDKESHHLFIEPQTIDATEYYINGFSTSLPYEVQTQMLH
SVKGFENAKITRFGYAIEYDYIEPTELKHTLELKKIKNLYCAGQINGTTGYEEAAVQGFMAGINASLSIDMKEPLILRRD
EAYIGVLIDDLVVKGTKEPYRMFTSRAEFRLLLREENAILRLGKYGYVLGLLSEQDFAYIQNIANNLQKGLEFLLSKEFT
PNNQNNAFLESLGEDKISSIVNFQKIVARASFDIEKLKKLDPMFETMDNYSLREILNEAKYCHYISMQKAQVEKMKNLSE
LKIPENFDFKSVSGLSNEVVEKLNHHKPPTIFAASQISGITPAALDILQIYIKMQKKKA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=638, Percent_Identity=40.5956112852665, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI19882217, Length=663, Percent_Identity=39.0648567119155, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI183227703, Length=678, Percent_Identity=38.2005899705015, Blast_Score=427, Evalue=1e-119,
Organism=Escherichia coli, GI2367273, Length=617, Percent_Identity=50.8914100486224, Blast_Score=607, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI17534255, Length=633, Percent_Identity=39.1785150078989, Blast_Score=418, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6321202, Length=633, Percent_Identity=41.2322274881517, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24658174, Length=638, Percent_Identity=41.8495297805643, Blast_Score=443, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69139; Mature: 69139

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS01280 GIDA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDVIVIGGGHAGVEASAAAARMGKKTLLLTTLIEQIGAASCNPAIGGLAKGHLVKELDA
CEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
MGGFMGEITDEAGIQFRILNESKGVAVQGSRVQIDMDKYRIIARNKLLKLPNLEISQEQV
HCCHHHHCCCCCCCEEEEEECCCCEEEECCEEEEEHHHEEEEEHHCEEECCCCCCCHHHH
SALIVENDKVKGVKTNLENIYFAKKVILTTGTFLNGLIHVGENKLQAGRVGELASVNLGN
HEEEEECCCCCCHHHCHHHHHHHHHHEEECCHHHHHHEECCCCCCCCCCCCCEEECCHHH
YLQTLGLKMGRLKTGTCPRVDATSIDFSVLEIQDGDVNPKAFSFRSRNFNPTQLPCYIAR
HHHHHCCHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCEEEEEE
TNTTTHEIIKNNFYRAPLFTGQIEGVGPRYCPSIEDKINRFSDKESHHLFIEPQTIDATE
CCCCHHHHHHCCCEECCEEECCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCHH
YYINGFSTSLPYEVQTQMLHSVKGFENAKITRFGYAIEYDYIEPTELKHTLELKKIKNLY
HEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCEE
CAGQINGTTGYEEAAVQGFMAGINASLSIDMKEPLILRRDEAYIGVLIDDLVVKGTKEPY
EEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEEEEEEHHHHHCCCCCHH
RMFTSRAEFRLLLREENAILRLGKYGYVLGLLSEQDFAYIQNIANNLQKGLEFLLSKEFT
HHHHCCHHEEEEEECCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PNNQNNAFLESLGEDKISSIVNFQKIVARASFDIEKLKKLDPMFETMDNYSLREILNEAK
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHCCCHHHHHHHHHH
YCHYISMQKAQVEKMKNLSELKIPENFDFKSVSGLSNEVVEKLNHHKPPTIFAASQISGI
HEEEHHHHHHHHHHHCCHHHCCCCCCCCCHHHCCCHHHHHHHHHCCCCCEEEEHHHCCCC
TPAALDILQIYIKMQKKKA
CHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFDVIVIGGGHAGVEASAAAARMGKKTLLLTTLIEQIGAASCNPAIGGLAKGHLVKELDA
CEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
MGGFMGEITDEAGIQFRILNESKGVAVQGSRVQIDMDKYRIIARNKLLKLPNLEISQEQV
HCCHHHHCCCCCCCEEEEEECCCCEEEECCEEEEEHHHEEEEEHHCEEECCCCCCCHHHH
SALIVENDKVKGVKTNLENIYFAKKVILTTGTFLNGLIHVGENKLQAGRVGELASVNLGN
HEEEEECCCCCCHHHCHHHHHHHHHHEEECCHHHHHHEECCCCCCCCCCCCCEEECCHHH
YLQTLGLKMGRLKTGTCPRVDATSIDFSVLEIQDGDVNPKAFSFRSRNFNPTQLPCYIAR
HHHHHCCHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCEEEEEE
TNTTTHEIIKNNFYRAPLFTGQIEGVGPRYCPSIEDKINRFSDKESHHLFIEPQTIDATE
CCCCHHHHHHCCCEECCEEECCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCHH
YYINGFSTSLPYEVQTQMLHSVKGFENAKITRFGYAIEYDYIEPTELKHTLELKKIKNLY
HEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCEE
CAGQINGTTGYEEAAVQGFMAGINASLSIDMKEPLILRRDEAYIGVLIDDLVVKGTKEPY
EEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEEEEEEHHHHHCCCCCHH
RMFTSRAEFRLLLREENAILRLGKYGYVLGLLSEQDFAYIQNIANNLQKGLEFLLSKEFT
HHHHCCHHEEEEEECCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PNNQNNAFLESLGEDKISSIVNFQKIVARASFDIEKLKKLDPMFETMDNYSLREILNEAK
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHCCCHHHHHHHHHH
YCHYISMQKAQVEKMKNLSELKIPENFDFKSVSGLSNEVVEKLNHHKPPTIFAASQISGI
HEEEHHHHHHHHHHHCCHHHCCCCCCCCCHHHCCCHHHHHHHHHCCCCCEEEEHHHCCCC
TPAALDILQIYIKMQKKKA
CHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA