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The map label for this gene is pilT [H]

Identifier: 153930595

GI number: 153930595

Start: 25039

End: 25992

Strand: Direct

Name: pilT [H]

Synonym: YpsIP31758_A0066

Alternate gene names: 153930595

Gene position: 25039-25992 (Clockwise)

Preceding gene: 153930579

Following gene: 153930572

Centisome position: 42.67

GC content: 37.11

Gene sequence:

>954_bases
GTGACAGAATCCTTGCTCAAAGATTCTGGCTTTGTTGATTTATATCTTGGTGATGATTATACCGATATAAAAGGCATGAT
AGGGGCAAGCAATAGGCTTGCCCCTGCGCCAGAAATATTAAAAAACGATATCGAGAGTTTAAGACAACAATGTTTGAATT
TTTATCATGCAGAAAAAAGGAATGAATTCTCTATTTTTTATGGTGAACGGTTGTACAGGGTAACAACAACTATTGATGTT
TATCAAAATATTGGTTTTGTTATCAGACAAACGCCGAATGAATTATTCACTCTACAAAAAGTAGGTCTATCATCTGATCT
TATAAAGCTCATCAGCCAGAATAATAATTCTGGTTTAGTTTTAGTTTCAGGAAAAATGGGAGCAGGGAAGACAACCACGG
CAGCGGCTATGATTTGCCACAGAATTAAGGAGAGCGGAAACCTTGGTGTTTCAATAGAAGATCCGATAGAAACATTACTC
GGTGGAAAACATGGTGATGGTCGCTGTATTCAACTGGAAGTCTCTGATAATGAGGGATACCCGCTAGCGATAAAGCGGGC
CTTGAGAATGGGGGCCAATAATATCCTCATAGGGGAAATACGAGACGCGCAGACAGCCCATGAAGTCATGAAAGCATCTC
TGAATGGTGCATTCATTGTTGCTACGATTCATGCGAATAATTGTATTGATGCAATTGAACGATATTCTATTTTCTGTAAT
GAAATAAACAGTAAAGCAAACTCGATAATTGCAAAATCACTTTTTATTGTCACTAATCAAGTGTTAATGCCATCTATATA
TGAAAGTAATATAAGAGGTTATACATGTACAGTGAATGGTTACGATATTCGCGCGAGTACAACTATTCAGGCAAAAATCA
GAGACGGAAATTTCCAAACCTTAAAAGATGAATTTGACAGCATCAATCAAAAGCAAATGCGACATTCTCTATAA

Upstream 100 bases:

>100_bases
CGTAACAAGTGAAATGTCACAAATAGATGCGGCTAATCAATGCAACGCAGGAAAAGAAAATACAATTTCAATTACTTCTT
ACTAATATATAAGGTCTATT

Downstream 100 bases:

>100_bases
TATGAGGTGGTAATGAAAAAATTTAAAAAAGGGTTGGCGGCGATATCAGGGCAAACAATCGCTTTAATTGCTGGCCTAAT
TGTACTGGCAACAATTACCG

Product: type IV secretion system protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MTESLLKDSGFVDLYLGDDYTDIKGMIGASNRLAPAPEILKNDIESLRQQCLNFYHAEKRNEFSIFYGERLYRVTTTIDV
YQNIGFVIRQTPNELFTLQKVGLSSDLIKLISQNNNSGLVLVSGKMGAGKTTTAAAMICHRIKESGNLGVSIEDPIETLL
GGKHGDGRCIQLEVSDNEGYPLAIKRALRMGANNILIGEIRDAQTAHEVMKASLNGAFIVATIHANNCIDAIERYSIFCN
EINSKANSIIAKSLFIVTNQVLMPSIYESNIRGYTCTVNGYDIRASTTIQAKIRDGNFQTLKDEFDSINQKQMRHSL

Sequences:

>Translated_317_residues
MTESLLKDSGFVDLYLGDDYTDIKGMIGASNRLAPAPEILKNDIESLRQQCLNFYHAEKRNEFSIFYGERLYRVTTTIDV
YQNIGFVIRQTPNELFTLQKVGLSSDLIKLISQNNNSGLVLVSGKMGAGKTTTAAAMICHRIKESGNLGVSIEDPIETLL
GGKHGDGRCIQLEVSDNEGYPLAIKRALRMGANNILIGEIRDAQTAHEVMKASLNGAFIVATIHANNCIDAIERYSIFCN
EINSKANSIIAKSLFIVTNQVLMPSIYESNIRGYTCTVNGYDIRASTTIQAKIRDGNFQTLKDEFDSINQKQMRHSL
>Mature_316_residues
TESLLKDSGFVDLYLGDDYTDIKGMIGASNRLAPAPEILKNDIESLRQQCLNFYHAEKRNEFSIFYGERLYRVTTTIDVY
QNIGFVIRQTPNELFTLQKVGLSSDLIKLISQNNNSGLVLVSGKMGAGKTTTAAAMICHRIKESGNLGVSIEDPIETLLG
GKHGDGRCIQLEVSDNEGYPLAIKRALRMGANNILIGEIRDAQTAHEVMKASLNGAFIVATIHANNCIDAIERYSIFCNE
INSKANSIIAKSLFIVTNQVLMPSIYESNIRGYTCTVNGYDIRASTTIQAKIRDGNFQTLKDEFDSINQKQMRHSL

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=200, Percent_Identity=28.5, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789723, Length=213, Percent_Identity=29.5774647887324, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 35130; Mature: 34998

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESLLKDSGFVDLYLGDDYTDIKGMIGASNRLAPAPEILKNDIESLRQQCLNFYHAEKR
CCCHHHCCCCEEEEEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
NEFSIFYGERLYRVTTTIDVYQNIGFVIRQTPNELFTLQKVGLSSDLIKLISQNNNSGLV
CCEEEEECCEEEEEEEHHHHHHCCCEEEECCCHHHEEHHHCCCCHHHHHHHHCCCCCCEE
LVSGKMGAGKTTTAAAMICHRIKESGNLGVSIEDPIETLLGGKHGDGRCIQLEVSDNEGY
EEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCEEEEEEECCCCCC
PLAIKRALRMGANNILIGEIRDAQTAHEVMKASLNGAFIVATIHANNCIDAIERYSIFCN
HHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHH
EINSKANSIIAKSLFIVTNQVLMPSIYESNIRGYTCTVNGYDIRASTTIQAKIRDGNFQT
HHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCEEEEEEEEEEEEEECCCCHH
LKDEFDSINQKQMRHSL
HHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
TESLLKDSGFVDLYLGDDYTDIKGMIGASNRLAPAPEILKNDIESLRQQCLNFYHAEKR
CCHHHCCCCEEEEEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
NEFSIFYGERLYRVTTTIDVYQNIGFVIRQTPNELFTLQKVGLSSDLIKLISQNNNSGLV
CCEEEEECCEEEEEEEHHHHHHCCCEEEECCCHHHEEHHHCCCCHHHHHHHHCCCCCCEE
LVSGKMGAGKTTTAAAMICHRIKESGNLGVSIEDPIETLLGGKHGDGRCIQLEVSDNEGY
EEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCEEEEEEECCCCCC
PLAIKRALRMGANNILIGEIRDAQTAHEVMKASLNGAFIVATIHANNCIDAIERYSIFCN
HHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHH
EINSKANSIIAKSLFIVTNQVLMPSIYESNIRGYTCTVNGYDIRASTTIQAKIRDGNFQT
HHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCEEEEEEEEEEEEEECCCCHH
LKDEFDSINQKQMRHSL
HHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]