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The map label for this gene is fieF [H]

Identifier: 153937860

GI number: 153937860

Start: 3438872

End: 3440050

Strand: Direct

Name: fieF [H]

Synonym: CLC_3284

Alternate gene names: 153937860

Gene position: 3438872-3440050 (Clockwise)

Preceding gene: 153937650

Following gene: 153935098

Centisome position: 91.45

GC content: 27.4

Gene sequence:

>1179_bases
ATGTTATCTAAACTTTTAGTAATTAAATTTGTAAAAGATTATAAAAACATTAACCATAAAGAAGTTAGAGATTCTTATGG
ATATTTGGCTAGTATAATTGGTATTCTAACAAATATATTTTTATTTATAATTAAAATTTCTGTAGGATTAATATCTCACA
GCATTGCAGTTACAGCGGATGCTTTCAACAATCTATCTGATGCTGCATCATCTATAATAACATTTTTAGGCTTTAAATTA
GCCTCTAAACCTGCAGATAAAGAACATCCCTTTGGGCATGGTAGGATTGAATACATATCTGGACTAATTGTATCTTTTAT
AGTACTTTTAGTTGGGCTTGAATTTATAAAGTCTTCCTTTGATAGAATAAAAAACCCTTCTATAGTAAAATTTCAATGGA
TACCATTTTTATTAATATTATTTTCCATTGTTATAAAACTTTGGCTTAGCCGTTTTAATAAATATATAGGTGATACTATT
AATTCTGGTGCACTTAAAGCCTCTTCTTTAGATGCATTAAGTGATGTTGTAACCTCTTCCTCTGTTGCCTTATCTTTATT
GTTAGCTAAGTGGATTTCCTTTCCTATAGATGGATATTTCGGAATATTAGTTTCTTTATTTATAATATATTCTGGTATTT
CACTTATAAAAGAAACTTTGGATCCTTTATTAGGTGAAGCTCCAGATAAAGAACTAGTATCTGAAATAATAGAAATGGTT
ATGAGTTATGAATTAATAATGGGAGTTCATGATTTAATAGTACACAATTATGGCCCCGGCAGATGTATGGCATCTATCCA
CGCAGAAGTTCCTTCAAATGAATCCATTGTTAAGATTCATGAAGTTATAGATAAAGCTGAAAATGAAATATCTAAAAAAT
TAGATCTTCTTCTAGTTATTCACATGGATCCTATTAATGTAGATAATAAAGAAGTAGCTTATACTCGTAAGGAAGTAGTT
AAAATTTTGGAAGGATTTAATATAATAAAATCTATGCATGATTTTAGAATGGTTGGAGAAGATGAATATAAAAACCTAAT
ATTTGATATAGTGATAGATCATTCTTTTAAGCTTACTTTAAAATTGGAAAATCAGCTTAAAAAAGATATAGATAATTGTA
TAAAAAAGTTACATCCTAAATACAATACAGTTATAACCATAGATAGAGATTTTTATTAA

Upstream 100 bases:

>100_bases
TATATCAATATATTATATGCTATAAAATATTTTAATATAGAAAACTTTACTAACAAATATGTTATAATACAGTTTATATT
GAAATATAGTAGGTGATATT

Downstream 100 bases:

>100_bases
CTTAACTTTTATTATCTTAATTTTTTTATTTCATATATAAATGCTCTAAAATCTATATATGCTAACTTTCATTAGTATAC
AAAATTATGTTTTGTGCATA

Product: cation efflux family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MLSKLLVIKFVKDYKNINHKEVRDSYGYLASIIGILTNIFLFIIKISVGLISHSIAVTADAFNNLSDAASSIITFLGFKL
ASKPADKEHPFGHGRIEYISGLIVSFIVLLVGLEFIKSSFDRIKNPSIVKFQWIPFLLILFSIVIKLWLSRFNKYIGDTI
NSGALKASSLDALSDVVTSSSVALSLLLAKWISFPIDGYFGILVSLFIIYSGISLIKETLDPLLGEAPDKELVSEIIEMV
MSYELIMGVHDLIVHNYGPGRCMASIHAEVPSNESIVKIHEVIDKAENEISKKLDLLLVIHMDPINVDNKEVAYTRKEVV
KILEGFNIIKSMHDFRMVGEDEYKNLIFDIVIDHSFKLTLKLENQLKKDIDNCIKKLHPKYNTVITIDRDFY

Sequences:

>Translated_392_residues
MLSKLLVIKFVKDYKNINHKEVRDSYGYLASIIGILTNIFLFIIKISVGLISHSIAVTADAFNNLSDAASSIITFLGFKL
ASKPADKEHPFGHGRIEYISGLIVSFIVLLVGLEFIKSSFDRIKNPSIVKFQWIPFLLILFSIVIKLWLSRFNKYIGDTI
NSGALKASSLDALSDVVTSSSVALSLLLAKWISFPIDGYFGILVSLFIIYSGISLIKETLDPLLGEAPDKELVSEIIEMV
MSYELIMGVHDLIVHNYGPGRCMASIHAEVPSNESIVKIHEVIDKAENEISKKLDLLLVIHMDPINVDNKEVAYTRKEVV
KILEGFNIIKSMHDFRMVGEDEYKNLIFDIVIDHSFKLTLKLENQLKKDIDNCIKKLHPKYNTVITIDRDFY
>Mature_392_residues
MLSKLLVIKFVKDYKNINHKEVRDSYGYLASIIGILTNIFLFIIKISVGLISHSIAVTADAFNNLSDAASSIITFLGFKL
ASKPADKEHPFGHGRIEYISGLIVSFIVLLVGLEFIKSSFDRIKNPSIVKFQWIPFLLILFSIVIKLWLSRFNKYIGDTI
NSGALKASSLDALSDVVTSSSVALSLLLAKWISFPIDGYFGILVSLFIIYSGISLIKETLDPLLGEAPDKELVSEIIEMV
MSYELIMGVHDLIVHNYGPGRCMASIHAEVPSNESIVKIHEVIDKAENEISKKLDLLLVIHMDPINVDNKEVAYTRKEVV
KILEGFNIIKSMHDFRMVGEDEYKNLIFDIVIDHSFKLTLKLENQLKKDIDNCIKKLHPKYNTVITIDRDFY

Specific function: Cation-efflux transporter that may have a role in detoxification [H]

COG id: COG0053

COG function: function code P; Predicted Co/Zn/Cd cation transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. FieF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790349, Length=274, Percent_Identity=27.007299270073, Blast_Score=110, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6325032, Length=243, Percent_Identity=26.3374485596708, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323831, Length=109, Percent_Identity=31.1926605504587, Blast_Score=63, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002524 [H]

Pfam domain/function: PF01545 Cation_efflux [H]

EC number: NA

Molecular weight: Translated: 44200; Mature: 44200

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKLLVIKFVKDYKNINHKEVRDSYGYLASIIGILTNIFLFIIKISVGLISHSIAVTAD
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHH
AFNNLSDAASSIITFLGFKLASKPADKEHPFGHGRIEYISGLIVSFIVLLVGLEFIKSSF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DRIKNPSIVKFQWIPFLLILFSIVIKLWLSRFNKYIGDTINSGALKASSLDALSDVVTSS
HHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
SVALSLLLAKWISFPIDGYFGILVSLFIIYSGISLIKETLDPLLGEAPDKELVSEIIEMV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
MSYELIMGVHDLIVHNYGPGRCMASIHAEVPSNESIVKIHEVIDKAENEISKKLDLLLVI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEE
HMDPINVDNKEVAYTRKEVVKILEGFNIIKSMHDFRMVGEDEYKNLIFDIVIDHSFKLTL
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCEEEEE
KLENQLKKDIDNCIKKLHPKYNTVITIDRDFY
EHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
>Mature Secondary Structure
MLSKLLVIKFVKDYKNINHKEVRDSYGYLASIIGILTNIFLFIIKISVGLISHSIAVTAD
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHH
AFNNLSDAASSIITFLGFKLASKPADKEHPFGHGRIEYISGLIVSFIVLLVGLEFIKSSF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DRIKNPSIVKFQWIPFLLILFSIVIKLWLSRFNKYIGDTINSGALKASSLDALSDVVTSS
HHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
SVALSLLLAKWISFPIDGYFGILVSLFIIYSGISLIKETLDPLLGEAPDKELVSEIIEMV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
MSYELIMGVHDLIVHNYGPGRCMASIHAEVPSNESIVKIHEVIDKAENEISKKLDLLLVI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEE
HMDPINVDNKEVAYTRKEVVKILEGFNIIKSMHDFRMVGEDEYKNLIFDIVIDHSFKLTL
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCEEEEE
KLENQLKKDIDNCIKKLHPKYNTVITIDRDFY
EHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA