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The map label for this gene is ytsJ [H]

Identifier: 153937723

GI number: 153937723

Start: 285174

End: 286346

Strand: Direct

Name: ytsJ [H]

Synonym: CLC_0304

Alternate gene names: 153937723

Gene position: 285174-286346 (Clockwise)

Preceding gene: 153934995

Following gene: 153936944

Centisome position: 7.58

GC content: 33.59

Gene sequence:

>1173_bases
ATGAATTATTTTGAAGAAAGCTTAAAACTACATGAAGATAATAGAGGAAAAATAGAAATAAAATCAAAGATGGAAGTTAA
AACAAGAGATGATTTAAGTTTGGCTTATACTCCTGGAGTTGCAGAGCCATGTAGAAAGATTCATGAAAACGAAGAAAATG
TTTATAAATATACTTCAAAAGGAAATATGGTTGCAGTAGTTACAGATGGAACGGCAGTATTAGGATTAGGAGATATAGGG
CCTAAGGCTGGATTACCAGTTATGGAAGGAAAAGCTCTTTTATTTAAAGAATTTGCAAATGTTGATGCATTTCCAATATG
TTTAGATACAAAAGATGTTGATGAAATAGTAAAGACTGTAAAACTTATAGCCCCAGGATTTGGTGGAATTAATTTAGAAG
ATATTGGAGCTCCAAGATGCTTTGAAATAGAAGAGAAGCTAAAGAAAGAACTTGATATACCTGTATTTCATGATGATCAA
CATGGAACTGCCATAGTTGTTCTTGCAGGGGTAATAAATGCTCTAAAAGTAGTTAATAAGAAAATAGAAGACGTAAAGGT
TGTTGTAAATGGTGCAGGAGCTGCAGGAACTGCCATAACTAAACTATTACTTTCTTTAGGAGTAAAAAATCTAATTGTAT
GTGATAAGGTTGGTATCCTTTATGAAGGAATAGAAAATGTAGATGATGCAAAGAAGGAACTTTCAAAGGTAACAAATCCA
GAAAATATTAAAGGAACTTTAGCTGATGCATTAATAGGTGCAGATGTATTTATAGGGGTTTCAGCTCCAGGAATAGTAAG
TAAAGATATGGTAGAAACAATGAATAAGGATTCCATAATATTTGCTATGGCAAATCCAACTCCAGAAATAATGCCAGATT
TGGCAAAGGAAGCAGGGGCTAAGGTAATTGGAACAGGAAGATCAGACTTCCCTAACCAAGTTAACAATGTGCTTGCATTC
CCAGGAATATTTAAGGGTGCATTGGATGTTAGAGCAAAAGAAATTAATGAAGAAATGAAAATAGCAGCAGCATATGCTAT
AGCTTCTATGATAAAAGATAAGGATTTAAATGAGGACAATGTAATTCCTTATGCATTAGATAGATCTGTTGCAGACAATG
TAGCAAAAGCAATAAAAGAAGCAGCGAGAAAAAGCGGAGTAGCTAGAGTATAA

Upstream 100 bases:

>100_bases
TTAAAGTTTAAAATATTTTAATAATTTAAACTTGATGTTATGATTTATTCAAAGCAAACGGTTACAGAAATCATATAAAA
AGTGAAAGGATGTTATAAGA

Downstream 100 bases:

>100_bases
ACAGAGTTCTTGACTTTAGAGGGAGTTTTTACTCCACCTAAAGCTTATTAACAGACTTCTTAGGAGTTTTACTACTTAGA
AGTCGTTATCCTTTAGGAGA

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MNYFEESLKLHEDNRGKIEIKSKMEVKTRDDLSLAYTPGVAEPCRKIHENEENVYKYTSKGNMVAVVTDGTAVLGLGDIG
PKAGLPVMEGKALLFKEFANVDAFPICLDTKDVDEIVKTVKLIAPGFGGINLEDIGAPRCFEIEEKLKKELDIPVFHDDQ
HGTAIVVLAGVINALKVVNKKIEDVKVVVNGAGAAGTAITKLLLSLGVKNLIVCDKVGILYEGIENVDDAKKELSKVTNP
ENIKGTLADALIGADVFIGVSAPGIVSKDMVETMNKDSIIFAMANPTPEIMPDLAKEAGAKVIGTGRSDFPNQVNNVLAF
PGIFKGALDVRAKEINEEMKIAAAYAIASMIKDKDLNEDNVIPYALDRSVADNVAKAIKEAARKSGVARV

Sequences:

>Translated_390_residues
MNYFEESLKLHEDNRGKIEIKSKMEVKTRDDLSLAYTPGVAEPCRKIHENEENVYKYTSKGNMVAVVTDGTAVLGLGDIG
PKAGLPVMEGKALLFKEFANVDAFPICLDTKDVDEIVKTVKLIAPGFGGINLEDIGAPRCFEIEEKLKKELDIPVFHDDQ
HGTAIVVLAGVINALKVVNKKIEDVKVVVNGAGAAGTAITKLLLSLGVKNLIVCDKVGILYEGIENVDDAKKELSKVTNP
ENIKGTLADALIGADVFIGVSAPGIVSKDMVETMNKDSIIFAMANPTPEIMPDLAKEAGAKVIGTGRSDFPNQVNNVLAF
PGIFKGALDVRAKEINEEMKIAAAYAIASMIKDKDLNEDNVIPYALDRSVADNVAKAIKEAARKSGVARV
>Mature_390_residues
MNYFEESLKLHEDNRGKIEIKSKMEVKTRDDLSLAYTPGVAEPCRKIHENEENVYKYTSKGNMVAVVTDGTAVLGLGDIG
PKAGLPVMEGKALLFKEFANVDAFPICLDTKDVDEIVKTVKLIAPGFGGINLEDIGAPRCFEIEEKLKKELDIPVFHDDQ
HGTAIVVLAGVINALKVVNKKIEDVKVVVNGAGAAGTAITKLLLSLGVKNLIVCDKVGILYEGIENVDDAKKELSKVTNP
ENIKGTLADALIGADVFIGVSAPGIVSKDMVETMNKDSIIFAMANPTPEIMPDLAKEAGAKVIGTGRSDFPNQVNNVLAF
PGIFKGALDVRAKEINEEMKIAAAYAIASMIKDKDLNEDNVIPYALDRSVADNVAKAIKEAARKSGVARV

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505145, Length=379, Percent_Identity=28.7598944591029, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI4505143, Length=389, Percent_Identity=25.4498714652956, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI270265879, Length=320, Percent_Identity=30, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI62420882, Length=384, Percent_Identity=25, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI62420880, Length=384, Percent_Identity=25, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI239049447, Length=384, Percent_Identity=25, Blast_Score=86, Evalue=7e-17,
Organism=Escherichia coli, GI1788806, Length=403, Percent_Identity=49.1315136476427, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI87081919, Length=445, Percent_Identity=24.9438202247191, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17537199, Length=440, Percent_Identity=25.4545454545455, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6322823, Length=389, Percent_Identity=27.5064267352185, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21356279, Length=437, Percent_Identity=25.1716247139588, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281362672, Length=437, Percent_Identity=25.1716247139588, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281362674, Length=437, Percent_Identity=25.1716247139588, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI78707242, Length=382, Percent_Identity=26.4397905759162, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI78707238, Length=382, Percent_Identity=26.4397905759162, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24646386, Length=441, Percent_Identity=26.984126984127, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24646388, Length=441, Percent_Identity=26.984126984127, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI78707240, Length=382, Percent_Identity=26.4397905759162, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281363505, Length=391, Percent_Identity=26.8542199488491, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI78707236, Length=391, Percent_Identity=26.8542199488491, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI78707232, Length=391, Percent_Identity=26.8542199488491, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281363503, Length=391, Percent_Identity=26.8542199488491, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 41875; Mature: 41875

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYFEESLKLHEDNRGKIEIKSKMEVKTRDDLSLAYTPGVAEPCRKIHENEENVYKYTSK
CCHHHHHHHHCCCCCCEEEEECCEEEECCCCCEEEECCCHHHHHHHHHCCCCCEEEEECC
GNMVAVVTDGTAVLGLGDIGPKAGLPVMEGKALLFKEFANVDAFPICLDTKDVDEIVKTV
CCEEEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHH
KLIAPGFGGINLEDIGAPRCFEIEEKLKKELDIPVFHDDQHGTAIVVLAGVINALKVVNK
HHHCCCCCCCCHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHH
KIEDVKVVVNGAGAAGTAITKLLLSLGVKNLIVCDKVGILYEGIENVDDAKKELSKVTNP
HHCEEEEEEECCCCHHHHHHHHHHHHCHHHEEEHHHHHHHHHHHCCHHHHHHHHHHCCCC
ENIKGTLADALIGADVFIGVSAPGIVSKDMVETMNKDSIIFAMANPTPEIMPDLAKEAGA
CCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCC
KVIGTGRSDFPNQVNNVLAFPGIFKGALDVRAKEINEEMKIAAAYAIASMIKDKDLNEDN
EEEECCCCCCCHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VIPYALDRSVADNVAKAIKEAARKSGVARV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNYFEESLKLHEDNRGKIEIKSKMEVKTRDDLSLAYTPGVAEPCRKIHENEENVYKYTSK
CCHHHHHHHHCCCCCCEEEEECCEEEECCCCCEEEECCCHHHHHHHHHCCCCCEEEEECC
GNMVAVVTDGTAVLGLGDIGPKAGLPVMEGKALLFKEFANVDAFPICLDTKDVDEIVKTV
CCEEEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHH
KLIAPGFGGINLEDIGAPRCFEIEEKLKKELDIPVFHDDQHGTAIVVLAGVINALKVVNK
HHHCCCCCCCCHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHH
KIEDVKVVVNGAGAAGTAITKLLLSLGVKNLIVCDKVGILYEGIENVDDAKKELSKVTNP
HHCEEEEEEECCCCHHHHHHHHHHHHCHHHEEEHHHHHHHHHHHCCHHHHHHHHHHCCCC
ENIKGTLADALIGADVFIGVSAPGIVSKDMVETMNKDSIIFAMANPTPEIMPDLAKEAGA
CCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCC
KVIGTGRSDFPNQVNNVLAFPGIFKGALDVRAKEINEEMKIAAAYAIASMIKDKDLNEDN
EEEECCCCCCCHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VIPYALDRSVADNVAKAIKEAARKSGVARV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]