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The map label for this gene is pgk

Identifier: 153937674

GI number: 153937674

Start: 261483

End: 262679

Strand: Direct

Name: pgk

Synonym: CLC_0283

Alternate gene names: 153937674

Gene position: 261483-262679 (Clockwise)

Preceding gene: 153936763

Following gene: 153935201

Centisome position: 6.95

GC content: 30.74

Gene sequence:

>1197_bases
ATGAATTATAACAAAAAAAGTATAGAAGATATAGATGTAAAAGGTAAAAAAGTATTAGTAAGATGCGACTTTAATGTACC
ACTAAACGAAGGAAAAATAACAGATGAAAATAGATTAGTAGGAGCACTTCCAACAATTAAATATTTAATGGAAAAGGGTG
CTAAAATTATACTTTGTTCCCATATGGGAAAACCAAAAGGAGAACCTAAAAAAGAACTTTCATTATTACCAGTAGCCAAA
AGATTATCAGAAATGTTAAATAAAGAAGTAATATTTGCAGATGATGATAATGTAGTTGGTGAAAATGCTAAAAAAGCAGT
AGAAGATATGAAAGATGGAGATGTAGTATTACTACAAAATACTAGATATAGAAAAGAAGAAACTAAAAATGAAGAAGTTT
TTTCAAAGGAATTAGCTTCTCTTGCAGATGTATTTGTAAATGATGCATTTGGAACAGCTCACAGAGCTCATTGTTCAACA
GTAGGGGTTACAAATTATCTAAAAGAAGCTGCTTGTGGATACTTAATACAAAAAGAATTAAAATTCTTAGGAAATGCAGT
AGAAAAACCAGAAAGACCATTTGTAGCAATATTAGGTGGAGCTAAAGTTTCAGATAAAATAAATGTTATAAACAATTTAC
TAGATAAAGTAGACACATTAATAATAGGTGGAGGAATGGGATATACATTCCTAAAAGCTCAAGGATATACTATAGGAAAT
TCTTTAGTAGAAGAAGATAAAGTAGAATATTCAAAGGAAATGATAGATAAAGCTAAAGAAAAAGGAGTAAATCTATTATT
ACCAATAGATAATGTAGTAGCTGATAAATTTGATAAAGATGCAAGTCCTGTTATAACTGAAGATCAAAATATTGGCGAAG
GATATATGGGATTAGACATAGGACCTAAAACAGCAAAAATATACTCAGATGCTATAAAATCAGCAAAAACAGTAGTATGG
AATGGACCTATGGGAGTTTTTGAATTTAAAAGTTTTGCAAATGGAACTATAGAAGTAGCTAAGGCTATGGCAGATTCTGA
TGCTGTAACAATAATAGGCGGAGGAGATAGTGCAGCGGCAGTAAATATTCTTGGATTTGGAGATAAGATGACTCATATTT
CTACAGGTGGTGGAGCTTCTCTAGAATTCTTAGAAGGAAAAGAACTACCAGGAATAGCAGCTCTTAACGATAAATAA

Upstream 100 bases:

>100_bases
ATTATAGCAATAAAGCTAAAATAGAATAAGATAGTATAGCATAAATAAATTAATTGTATATACTAATAACCCCATAATAT
TATATAAGAGGTGAATGTCA

Downstream 100 bases:

>100_bases
AAGACAGAAAATAAATAGTTACACAGTATTACTTATAAAATGTATTGGAGCGTGATTTTAAATGAGAACAGCTATAATAG
CAGGAAACTGGAAGATGAAC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNYNKKSIEDIDVKGKKVLVRCDFNVPLNEGKITDENRLVGALPTIKYLMEKGAKIILCSHMGKPKGEPKKELSLLPVAK
RLSEMLNKEVIFADDDNVVGENAKKAVEDMKDGDVVLLQNTRYRKEETKNEEVFSKELASLADVFVNDAFGTAHRAHCST
VGVTNYLKEAACGYLIQKELKFLGNAVEKPERPFVAILGGAKVSDKINVINNLLDKVDTLIIGGGMGYTFLKAQGYTIGN
SLVEEDKVEYSKEMIDKAKEKGVNLLLPIDNVVADKFDKDASPVITEDQNIGEGYMGLDIGPKTAKIYSDAIKSAKTVVW
NGPMGVFEFKSFANGTIEVAKAMADSDAVTIIGGGDSAAAVNILGFGDKMTHISTGGGASLEFLEGKELPGIAALNDK

Sequences:

>Translated_398_residues
MNYNKKSIEDIDVKGKKVLVRCDFNVPLNEGKITDENRLVGALPTIKYLMEKGAKIILCSHMGKPKGEPKKELSLLPVAK
RLSEMLNKEVIFADDDNVVGENAKKAVEDMKDGDVVLLQNTRYRKEETKNEEVFSKELASLADVFVNDAFGTAHRAHCST
VGVTNYLKEAACGYLIQKELKFLGNAVEKPERPFVAILGGAKVSDKINVINNLLDKVDTLIIGGGMGYTFLKAQGYTIGN
SLVEEDKVEYSKEMIDKAKEKGVNLLLPIDNVVADKFDKDASPVITEDQNIGEGYMGLDIGPKTAKIYSDAIKSAKTVVW
NGPMGVFEFKSFANGTIEVAKAMADSDAVTIIGGGDSAAAVNILGFGDKMTHISTGGGASLEFLEGKELPGIAALNDK
>Mature_398_residues
MNYNKKSIEDIDVKGKKVLVRCDFNVPLNEGKITDENRLVGALPTIKYLMEKGAKIILCSHMGKPKGEPKKELSLLPVAK
RLSEMLNKEVIFADDDNVVGENAKKAVEDMKDGDVVLLQNTRYRKEETKNEEVFSKELASLADVFVNDAFGTAHRAHCST
VGVTNYLKEAACGYLIQKELKFLGNAVEKPERPFVAILGGAKVSDKINVINNLLDKVDTLIIGGGMGYTFLKAQGYTIGN
SLVEEDKVEYSKEMIDKAKEKGVNLLLPIDNVVADKFDKDASPVITEDQNIGEGYMGLDIGPKTAKIYSDAIKSAKTVVW
NGPMGVFEFKSFANGTIEVAKAMADSDAVTIIGGGDSAAAVNILGFGDKMTHISTGGGASLEFLEGKELPGIAALNDK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=417, Percent_Identity=47.9616306954436, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI31543397, Length=421, Percent_Identity=45.3681710213777, Blast_Score=347, Evalue=1e-95,
Organism=Escherichia coli, GI1789294, Length=394, Percent_Identity=43.4010152284264, Blast_Score=325, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17508823, Length=417, Percent_Identity=49.400479616307, Blast_Score=392, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6319857, Length=416, Percent_Identity=49.0384615384615, Blast_Score=385, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17136394, Length=417, Percent_Identity=47.242206235012, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=46.2650602409639, Blast_Score=353, Evalue=1e-97,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_CLOB1 (A7FQN7)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001382631.1
- ProteinModelPortal:   A7FQN7
- SMR:   A7FQN7
- STRING:   A7FQN7
- GeneID:   5394884
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0268
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   CBOT441770:CLB_0268-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 43063; Mature: 43063

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 38-38 BINDING 122-122 BINDING 155-155 BINDING 206-206 BINDING 297-297 BINDING 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYNKKSIEDIDVKGKKVLVRCDFNVPLNEGKITDENRLVGALPTIKYLMEKGAKIILCS
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCEEEEEE
HMGKPKGEPKKELSLLPVAKRLSEMLNKEVIFADDDNVVGENAKKAVEDMKDGDVVLLQN
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCCCCCEEEEEC
TRYRKEETKNEEVFSKELASLADVFVNDAFGTAHRAHCSTVGVTNYLKEAACGYLIQKEL
CCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHH
KFLGNAVEKPERPFVAILGGAKVSDKINVINNLLDKVDTLIIGGGMGYTFLKAQGYTIGN
HHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCC
SLVEEDKVEYSKEMIDKAKEKGVNLLLPIDNVVADKFDKDASPVITEDQNIGEGYMGLDI
HHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCCCCCEECCCCCCCCEEECCC
GPKTAKIYSDAIKSAKTVVWNGPMGVFEFKSFANGTIEVAKAMADSDAVTIIGGGDSAAA
CCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCEE
VNILGFGDKMTHISTGGGASLEFLEGKELPGIAALNDK
EEEEECCCCEEEEECCCCCEEEEECCCCCCCEECCCCC
>Mature Secondary Structure
MNYNKKSIEDIDVKGKKVLVRCDFNVPLNEGKITDENRLVGALPTIKYLMEKGAKIILCS
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCEEEEEE
HMGKPKGEPKKELSLLPVAKRLSEMLNKEVIFADDDNVVGENAKKAVEDMKDGDVVLLQN
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCCCCCEEEEEC
TRYRKEETKNEEVFSKELASLADVFVNDAFGTAHRAHCSTVGVTNYLKEAACGYLIQKEL
CCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHH
KFLGNAVEKPERPFVAILGGAKVSDKINVINNLLDKVDTLIIGGGMGYTFLKAQGYTIGN
HHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCC
SLVEEDKVEYSKEMIDKAKEKGVNLLLPIDNVVADKFDKDASPVITEDQNIGEGYMGLDI
HHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCCCCCEECCCCCCCCEEECCC
GPKTAKIYSDAIKSAKTVVWNGPMGVFEFKSFANGTIEVAKAMADSDAVTIIGGGDSAAA
CCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCEE
VNILGFGDKMTHISTGGGASLEFLEGKELPGIAALNDK
EEEEECCCCEEEEECCCCCEEEEECCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA