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The map label for this gene is gadB [H]

Identifier: 153937651

GI number: 153937651

Start: 1965838

End: 1967241

Strand: Direct

Name: gadB [H]

Synonym: CLC_1861

Alternate gene names: 153937651

Gene position: 1965838-1967241 (Clockwise)

Preceding gene: 153935153

Following gene: 153936951

Centisome position: 52.28

GC content: 31.48

Gene sequence:

>1404_bases
ATGCTCTATTCTTCAAAAAACAAGCAAAGTAATGATACCTATGCTACCCCTATTTTTGGTATCACTAAGGACAAGTATTC
AATTCCTAAGTACAAAATTAATGAAAATTCTATTGCACCTAATATAGCCTATCGTATGATTAAAGATGAACTTATGAATG
AAGGTAACGCACGCTTAAATTTAGCTACTTTTTGTCAGACCTATATGGAAGATAAAGCTACCAAACTTATGGCTGAAACC
TTACAAAAAAATGCTATCGACAAGTCTGAATATCCACAAACTGCCGAAATAGAGAATCGCTGTGTTAATATTATTTCAGA
TCTTTGGAATGTCCCAAAGGATATGAACTTCTTAGGTACCTCTACTGTGGGTTCTTCAGAAGCTTGTATGTTAGGTGGCA
TGTCTATGAAATTCCGTTGGCGTGATCAGGCTAAAAAATTAGGTATTGATATTAACAAGAAGAAACCTAACTTGGTAATA
TCATCTGGTTATCAGGTATGTTGGGAAAAATTCTGTGTATATTGGGATATTGAAATGCGTACCGTTCCTATGGATGAGGA
TAATCTAAGTTTAAATATAGATAAGATTCTTGATTATGTAGATGAATATACTATAGGAATTATAGGTATTTTAGGTATAA
CTTATACGGGTAAATTTGATGATATTAAAGCATTAGATGATGCCATTGAAAAATATAATAGTAATCATGATATAAAGGTA
TATATTCATGTAGATGCTGCCTCTGGTGGCTTTTTCACCCCATTTATTAATCCAGAAATTTTGTGGGATTTTCGTCTAAA
AAATGTAGTTTCTATAAATGCATCCGGTCATAAATATGGATTAGTGTATCCAGGAATAGGTTGGATTCTGTGGAAGGATC
AAGAATATCTTCCTAAAGATTTAATATTTGAAGTAAGTTATTTGGGTGGCAAAATGCCAACCTTAGCTATTAATTTTTCT
AGATCTGGAAGTCAAATCATAGGACAATATTATAATTTCCTACGCTTTGGTTTTGAAGGATATAAAAAAATTCATGAACG
CACAAAAGAGGTAGCTATGTATATTTCAAAAGAGCTTGAAGCTACAGGATTATTCAGTATATATAATGATGGCAGTAATC
TTCCTATAGTTTGTTATAAACTAAAGGAACAATCTAAGGTAAAATGGAATCTTTATGATTTATCAGATAGACTAGCTATG
AAAGGCTGGCAAATACCAGCTTACCCTCTCCCTGAAAATCTTAACCATATAATAATACAAAGAATAGTTTGTCGTTCAGA
CCTAGGATATAATTTAGCAGAACTATTGATAAAAGATTTTAAAACAGCAATAAATGATTTGAATAATGCTCATATATTAT
TTCATGAAGAAGAAAACCAAGGTATTTATGGATTTACTCATTAA

Upstream 100 bases:

>100_bases
ATTTTTTCTTTACCATTATAATATTACTAACAATTGATTTTTTTAGAAATTTTTAACAAATTAATTTTATATATTTAACT
TTAAGGAGGTTTTTTTTATT

Downstream 100 bases:

>100_bases
TATAAAGAAAACATGTATAAATTAAATAAATTTAATTTAAATATAATAAATACCTATGAATAAACTCATAGAAATATGTT
TTAGTAGCGTTTAATTTACT

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD-beta [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MLYSSKNKQSNDTYATPIFGITKDKYSIPKYKINENSIAPNIAYRMIKDELMNEGNARLNLATFCQTYMEDKATKLMAET
LQKNAIDKSEYPQTAEIENRCVNIISDLWNVPKDMNFLGTSTVGSSEACMLGGMSMKFRWRDQAKKLGIDINKKKPNLVI
SSGYQVCWEKFCVYWDIEMRTVPMDEDNLSLNIDKILDYVDEYTIGIIGILGITYTGKFDDIKALDDAIEKYNSNHDIKV
YIHVDAASGGFFTPFINPEILWDFRLKNVVSINASGHKYGLVYPGIGWILWKDQEYLPKDLIFEVSYLGGKMPTLAINFS
RSGSQIIGQYYNFLRFGFEGYKKIHERTKEVAMYISKELEATGLFSIYNDGSNLPIVCYKLKEQSKVKWNLYDLSDRLAM
KGWQIPAYPLPENLNHIIIQRIVCRSDLGYNLAELLIKDFKTAINDLNNAHILFHEEENQGIYGFTH

Sequences:

>Translated_467_residues
MLYSSKNKQSNDTYATPIFGITKDKYSIPKYKINENSIAPNIAYRMIKDELMNEGNARLNLATFCQTYMEDKATKLMAET
LQKNAIDKSEYPQTAEIENRCVNIISDLWNVPKDMNFLGTSTVGSSEACMLGGMSMKFRWRDQAKKLGIDINKKKPNLVI
SSGYQVCWEKFCVYWDIEMRTVPMDEDNLSLNIDKILDYVDEYTIGIIGILGITYTGKFDDIKALDDAIEKYNSNHDIKV
YIHVDAASGGFFTPFINPEILWDFRLKNVVSINASGHKYGLVYPGIGWILWKDQEYLPKDLIFEVSYLGGKMPTLAINFS
RSGSQIIGQYYNFLRFGFEGYKKIHERTKEVAMYISKELEATGLFSIYNDGSNLPIVCYKLKEQSKVKWNLYDLSDRLAM
KGWQIPAYPLPENLNHIIIQRIVCRSDLGYNLAELLIKDFKTAINDLNNAHILFHEEENQGIYGFTH
>Mature_467_residues
MLYSSKNKQSNDTYATPIFGITKDKYSIPKYKINENSIAPNIAYRMIKDELMNEGNARLNLATFCQTYMEDKATKLMAET
LQKNAIDKSEYPQTAEIENRCVNIISDLWNVPKDMNFLGTSTVGSSEACMLGGMSMKFRWRDQAKKLGIDINKKKPNLVI
SSGYQVCWEKFCVYWDIEMRTVPMDEDNLSLNIDKILDYVDEYTIGIIGILGITYTGKFDDIKALDDAIEKYNSNHDIKV
YIHVDAASGGFFTPFINPEILWDFRLKNVVSINASGHKYGLVYPGIGWILWKDQEYLPKDLIFEVSYLGGKMPTLAINFS
RSGSQIIGQYYNFLRFGFEGYKKIHERTKEVAMYISKELEATGLFSIYNDGSNLPIVCYKLKEQSKVKWNLYDLSDRLAM
KGWQIPAYPLPENLNHIIIQRIVCRSDLGYNLAELLIKDFKTAINDLNNAHILFHEEENQGIYGFTH

Specific function: Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance in gastric fluid [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=431, Percent_Identity=22.2737819025522, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1787769, Length=414, Percent_Identity=46.3768115942029, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1789934, Length=414, Percent_Identity=46.3768115942029, Blast_Score=418, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17543922, Length=326, Percent_Identity=23.3128834355828, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17557272, Length=357, Percent_Identity=25.4901960784314, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25148342, Length=320, Percent_Identity=23.4375, Blast_Score=92, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323905, Length=490, Percent_Identity=36.734693877551, Blast_Score=306, Evalue=5e-84,
Organism=Saccharomyces cerevisiae, GI6320500, Length=350, Percent_Identity=23.1428571428571, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21355963, Length=389, Percent_Identity=24.6786632390745, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24654344, Length=389, Percent_Identity=24.6786632390745, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 53660; Mature: 53660

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYSSKNKQSNDTYATPIFGITKDKYSIPKYKINENSIAPNIAYRMIKDELMNEGNARLN
CCCCCCCCCCCCCEECCEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCEEE
LATFCQTYMEDKATKLMAETLQKNAIDKSEYPQTAEIENRCVNIISDLWNVPKDMNFLGT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
STVGSSEACMLGGMSMKFRWRDQAKKLGIDINKKKPNLVISSGYQVCWEKFCVYWDIEMR
CCCCCCCCEEECCCEEEEEEHHHHHHCCCEECCCCCCEEEECCHHHHHHCEEEEEEEEEE
TVPMDEDNLSLNIDKILDYVDEYTIGIIGILGITYTGKFDDIKALDDAIEKYNSNHDIKV
EECCCCCCCEEEHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEE
YIHVDAASGGFFTPFINPEILWDFRLKNVVSINASGHKYGLVYPGIGWILWKDQEYLPKD
EEEEEECCCCEECCCCCCEEEEEEEECEEEEECCCCCEEEEEECCCEEEEECCCCCCCHH
LIFEVSYLGGKMPTLAINFSRSGSQIIGQYYNFLRFGFEGYKKIHERTKEVAMYISKELE
HHEEHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ATGLFSIYNDGSNLPIVCYKLKEQSKVKWNLYDLSDRLAMKGWQIPAYPLPENLNHIIIQ
CCEEEEEEECCCCCCEEEEEECCCCCEEEEEEECCHHHHHCCCCCCCCCCCCCCHHHHHH
RIVCRSDLGYNLAELLIKDFKTAINDLNNAHILFHEEENQGIYGFTH
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCC
>Mature Secondary Structure
MLYSSKNKQSNDTYATPIFGITKDKYSIPKYKINENSIAPNIAYRMIKDELMNEGNARLN
CCCCCCCCCCCCCEECCEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCEEE
LATFCQTYMEDKATKLMAETLQKNAIDKSEYPQTAEIENRCVNIISDLWNVPKDMNFLGT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
STVGSSEACMLGGMSMKFRWRDQAKKLGIDINKKKPNLVISSGYQVCWEKFCVYWDIEMR
CCCCCCCCEEECCCEEEEEEHHHHHHCCCEECCCCCCEEEECCHHHHHHCEEEEEEEEEE
TVPMDEDNLSLNIDKILDYVDEYTIGIIGILGITYTGKFDDIKALDDAIEKYNSNHDIKV
EECCCCCCCEEEHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEE
YIHVDAASGGFFTPFINPEILWDFRLKNVVSINASGHKYGLVYPGIGWILWKDQEYLPKD
EEEEEECCCCEECCCCCCEEEEEEEECEEEEECCCCCEEEEEECCCEEEEECCCCCCCHH
LIFEVSYLGGKMPTLAINFSRSGSQIIGQYYNFLRFGFEGYKKIHERTKEVAMYISKELE
HHEEHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ATGLFSIYNDGSNLPIVCYKLKEQSKVKWNLYDLSDRLAMKGWQIPAYPLPENLNHIIIQ
CCEEEEEEECCCCCCEEEEEECCCCCEEEEEEECCHHHHHCCCCCCCCCCCCCCHHHHHH
RIVCRSDLGYNLAELLIKDFKTAINDLNNAHILFHEEENQGIYGFTH
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]