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The map label for this gene is fruK [H]

Identifier: 153937463

GI number: 153937463

Start: 2058867

End: 2059790

Strand: Reverse

Name: fruK [H]

Synonym: CLC_1938

Alternate gene names: 153937463

Gene position: 2059790-2058867 (Counterclockwise)

Preceding gene: 153936491

Following gene: 153935815

Centisome position: 54.77

GC content: 28.68

Gene sequence:

>924_bases
ATGATTTATACATTAACTACTAATCCAGCTATTGATATGAACTGTTCTTCCAGTAGTAATGAAGTTTTTAAGGTAAATAG
AACTAATAATTTATCTTATTCGCCTAATGGAAAAGGAGTAAACGTATCGCTAGTTTTAAATCATTATTCTATAGAAAGCA
AAGCTCTTGGATTTTTCGGAGGATTTACAGGAAAGTATATTGTAGAGGAATTAAATAAGAAAAATATACTTACAGAAGCC
TTTTGGATAGAAGAAAATACAAGGATAAATGTATTTATAAATACGGAAGATAATAAAGAATTTAAGTATGTAAACAAGGG
ACCTTTTGTTTCAAGGGAAACCCAAAAGGATTTATTAAAATACTTATCATCAAATTTAAAAAAAGGTGATTATTTAATAA
TAAGTGGAAGTCTTCCCCAAAGTATTCAAGAGGATTACTACAATGAAATTTTAGATATATGTATGGAGAATAATGTTCAA
GTTATATTAGATATAAGCTCTAAAAAATTAAAAGAATTATTGAAATATAAACCTTTATTAATTAAACCTAATGATGAAGA
AGTTGAGGCAATATTTGGTCTAAGTTTAAAAGATGAAGAGGATATAAAAGAGGCATTAAAATATATTTATAAGTTAGGTG
CTAAAAATATACTTCTGACCTTAGGGGAGAAAGGAATGTATTTTTACAATGGAGACAAGATTTATTATTGCAATGCACCT
AAAATTAAACTTTTAAGCTCTGCTTGTGCAGGGGACTCGGCCTTAGCAGCATTTTTAAGTGAATGGTTATATGAAGCTAA
TATAGAAAAGGCACTTAAAAAGGCATCAGCCACTGGTGCAAATGTTGCTGAATCTAGTGCATTAGGAACTTTTGGTAAAG
TATCAGAGTATATAAAAAAAATACATGTTAAGGAGGTAGCGTAA

Upstream 100 bases:

>100_bases
TATATGACACCAGGAAAAGAAGTTATGAGAGAAGTAGTAAGGCATAAAATAATGGTTTGTGGAAGTAATGGACAAGCTTA
GACCTAGAGGAGATGAGGCT

Downstream 100 bases:

>100_bases
ATGAAAAAGATTATTGCTGTTACGGGATGTCCTACAGGAATAGCTCATACATTTATGGCAGAAGAAGCATTAAAGAAAGC
AGCTAAAGAATTAGGAGTAG

Product: PfkB family kinase

Products: NA

Alternate protein names: Fructose 1-phosphate kinase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MIYTLTTNPAIDMNCSSSSNEVFKVNRTNNLSYSPNGKGVNVSLVLNHYSIESKALGFFGGFTGKYIVEELNKKNILTEA
FWIEENTRINVFINTEDNKEFKYVNKGPFVSRETQKDLLKYLSSNLKKGDYLIISGSLPQSIQEDYYNEILDICMENNVQ
VILDISSKKLKELLKYKPLLIKPNDEEVEAIFGLSLKDEEDIKEALKYIYKLGAKNILLTLGEKGMYFYNGDKIYYCNAP
KIKLLSSACAGDSALAAFLSEWLYEANIEKALKKASATGANVAESSALGTFGKVSEYIKKIHVKEVA

Sequences:

>Translated_307_residues
MIYTLTTNPAIDMNCSSSSNEVFKVNRTNNLSYSPNGKGVNVSLVLNHYSIESKALGFFGGFTGKYIVEELNKKNILTEA
FWIEENTRINVFINTEDNKEFKYVNKGPFVSRETQKDLLKYLSSNLKKGDYLIISGSLPQSIQEDYYNEILDICMENNVQ
VILDISSKKLKELLKYKPLLIKPNDEEVEAIFGLSLKDEEDIKEALKYIYKLGAKNILLTLGEKGMYFYNGDKIYYCNAP
KIKLLSSACAGDSALAAFLSEWLYEANIEKALKKASATGANVAESSALGTFGKVSEYIKKIHVKEVA
>Mature_307_residues
MIYTLTTNPAIDMNCSSSSNEVFKVNRTNNLSYSPNGKGVNVSLVLNHYSIESKALGFFGGFTGKYIVEELNKKNILTEA
FWIEENTRINVFINTEDNKEFKYVNKGPFVSRETQKDLLKYLSSNLKKGDYLIISGSLPQSIQEDYYNEILDICMENNVQ
VILDISSKKLKELLKYKPLLIKPNDEEVEAIFGLSLKDEEDIKEALKYIYKLGAKNILLTLGEKGMYFYNGDKIYYCNAP
KIKLLSSACAGDSALAAFLSEWLYEANIEKALKKASATGANVAESSALGTFGKVSEYIKKIHVKEVA

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=288, Percent_Identity=25, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788493, Length=280, Percent_Identity=27.5, Blast_Score=100, Evalue=9e-23,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.56 [H]

Molecular weight: Translated: 34475; Mature: 34475

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYTLTTNPAIDMNCSSSSNEVFKVNRTNNLSYSPNGKGVNVSLVLNHYSIESKALGFFG
CEEEEECCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHC
GFTGKYIVEELNKKNILTEAFWIEENTRINVFINTEDNKEFKYVNKGPFVSRETQKDLLK
CCCHHHHHHHHCCCCCEEEEEEEECCCEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHH
YLSSNLKKGDYLIISGSLPQSIQEDYYNEILDICMENNVQVILDISSKKLKELLKYKPLL
HHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCEE
IKPNDEEVEAIFGLSLKDEEDIKEALKYIYKLGAKNILLTLGEKGMYFYNGDKIYYCNAP
ECCCCHHHHHEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCEEEEECCC
KIKLLSSACAGDSALAAFLSEWLYEANIEKALKKASATGANVAESSALGTFGKVSEYIKK
CEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHH
IHVKEVA
HHHHCCC
>Mature Secondary Structure
MIYTLTTNPAIDMNCSSSSNEVFKVNRTNNLSYSPNGKGVNVSLVLNHYSIESKALGFFG
CEEEEECCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHC
GFTGKYIVEELNKKNILTEAFWIEENTRINVFINTEDNKEFKYVNKGPFVSRETQKDLLK
CCCHHHHHHHHCCCCCEEEEEEEECCCEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHH
YLSSNLKKGDYLIISGSLPQSIQEDYYNEILDICMENNVQVILDISSKKLKELLKYKPLL
HHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCEE
IKPNDEEVEAIFGLSLKDEEDIKEALKYIYKLGAKNILLTLGEKGMYFYNGDKIYYCNAP
ECCCCHHHHHEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCEEEEECCC
KIKLLSSACAGDSALAAFLSEWLYEANIEKALKKASATGANVAESSALGTFGKVSEYIKK
CEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHH
IHVKEVA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]