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The map label for this gene is cadA [H]

Identifier: 153937186

GI number: 153937186

Start: 559689

End: 561917

Strand: Direct

Name: cadA [H]

Synonym: CLC_0551

Alternate gene names: 153937186

Gene position: 559689-561917 (Clockwise)

Preceding gene: 153936771

Following gene: 153935319

Centisome position: 14.88

GC content: 29.7

Gene sequence:

>2229_bases
ATGATTATAAAAAAGAAATTGCTTTTAAAAGGCTTACATTGTGCCAATTGTGCAACAAAAATAGAACGAGCCGTGCAAAA
GCTAAATATAATAGAGGAAGCTAACTATAATTTTAATAATTCAACTTTAATTATTAACTTAGAAGAAACTCATAAAGATA
GCATTATAAAAACAATACAAGAAATAGTAGATAGAATAGAACCCGGGGTAAAGGTAGTAGATAAAGAAAATTTAAAAAGA
ACGGTAGTGCATGCCCCTGTTAAAAATAGTAGTAATTTAAAGATGCAGAATAATAAAGAGGAAAATTTAAAATTAGATAA
AAAAGAAAATAGTTATGAGCATAATCATGATCATAGTCATGGACACAGTCATGATGGAGAAGATAGTGACGAGTTAGAAA
AGAAGACTCTTATAAGAATTATTTCAGGAGTGGTTTTATTAATTTTAGCTACCGTATTGAAATCTAAAGAAACTTTGTCT
ATAGGTCTATATTTAACTTCATATGTATTAATTGGAGGTAAGGTAATATTAAGTTCTATAAGAAATATATCTAAAGGACA
AGTATTTGATGAAAACTTTTTAATGGCTGTTGCAACTGTAGCGGCTATAGGAGTAAAACAGTATCCAGAAGCAGTAGCGG
TTATGCTATTTTATGAAGTAGGGGAGTTCTTACAAGATAAAGCTGTTAATAAATCTAGAAAATCTATAACAGCACTTATG
AATATAAGACCTGACTATGCCAATTTAGTTAGAGGTGAAGATATAGAAGTAGTTTCTCCAGAAGATATTAATATAGATGA
TATTATAATGGTAAAACCAGGAGAAAAAATTCCTCTAGATGGTATAGTTGTAGAAGGACAATCTTTTGTAGATACATCAG
CTATAACAGGTGAATCTTTAATAAGTGAAGTTTCAAAAGATAGTAATGTATTAAGTGGATATATAAACAAAAATGGTGTT
ATAAAAATAAAAGTTACAAAAACCTTTGGAGAATCTACAGTGTCCAAAATATTGGAATTAACAGAAAACGCATCAGCTAC
TAAGGCAAATACAGAAAAATTTATAACAAAGTTCGCCAGATATTATACTCCTGTAGTTGTTTTTGCTGCTTTAGCTTTAG
CAGTAATTCCTACACTAATACTAAAAGATCCAGATATATCTAAATGGATTTATAGAGCAGCTGTATTTTTAGTAGTATCT
TGTCCATGTGCTTTAGTAATATCAATACCTTTAAGCTTCTTTGCTGGTATTGGAGGGGCATCTAAAAAAGGTGTTTTAAT
AAAAGCAGGAACAGCATTAGAAGCTCTAAATGATGCAGATACAATAGTATTTGATAAAACAGGAACTTTAACTAAAGGTG
TGTTTAAAGTATCAAAAATTGATTCAGAAGAAGGAGTAAATACAGAAGAATTAATAGAATATGCTGCTTATGTAGAAAGT
TATTCTAATCACCCTATCGCAAAATCTATACTAAAGTATTATGAAAAAACCATAGATAATAAAAGAATAGAAGGTTATGA
AGAAATAGTTGCAAGAGGTGTAACTGCTTATATTGATGGCAAAAAAGTTTATGCTGGTAACAATAAATTAATGGAAGAAT
TAAATATTAACTATAAAAAAGCTCAAGAGGATGGAGTAATCCTTTATATAGCATTAGAAGATAAGTATATAGGTTATATA
GTTATAAACGACGAAATAAAGAAAGATTCAAAAGAAACAATAAAATCATTAAAAGATATAGGTATAAAAAAAGCAGCTAT
GCTTACAGGAGATAGAAAATCTACAGCTAATAATATAGGGACTTTTCTAGGAATGGATGAAATATACTCAGAACTTTTAC
CACAGGAAAAGGTAGAAAAGATGCAATCCTTAAAGTCCAAAACATCTAAAGATGGGAAAATTGTTTTTGTAGGGGATGGA
GTAAATGATGCTCCAGTATTAGCTATGTCAGATATAGGGGTATCCATGGGAGGCTTAGGATCTGATGCAGCCATAGAAGC
ATCTGATTTAGTTTTAATGTCTGATGAACCATCAAAATTAGTAGATGCCATAAAAATAGCTAGAAAAACTCATAAAATAG
TATGGCAAAATATAATTATAGTTTTAATAATCAAGTTTGCAGTATTAGCTCTAGCTGTAATGGGAAAAAGTACTATGTGG
ATGGCTGTTTTTGCCGATGTAGGTGTGGCTTTAATAGCAGTAATAAATGCATTAAGAATACTAAAATAA

Upstream 100 bases:

>100_bases
ACTAGAACACATAATGCATAGATAAAATTAAGTTTAATATTTTATTGTAATTTTAGAAATTTTGATTTTTTAATAACTAT
TTTAGAAGGATGGTGGGATC

Downstream 100 bases:

>100_bases
GAATTTACTAAAATAATAACTTTCTAAAGAAGGATAGGAACGAATAATTTTAAAGAAAAATATTTAATTATATTTATATA
GTTTTAGTTAAGTAGTTTTA

Product: cadmium-translocating P-type ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 742; Mature: 742

Protein sequence:

>742_residues
MIIKKKLLLKGLHCANCATKIERAVQKLNIIEEANYNFNNSTLIINLEETHKDSIIKTIQEIVDRIEPGVKVVDKENLKR
TVVHAPVKNSSNLKMQNNKEENLKLDKKENSYEHNHDHSHGHSHDGEDSDELEKKTLIRIISGVVLLILATVLKSKETLS
IGLYLTSYVLIGGKVILSSIRNISKGQVFDENFLMAVATVAAIGVKQYPEAVAVMLFYEVGEFLQDKAVNKSRKSITALM
NIRPDYANLVRGEDIEVVSPEDINIDDIIMVKPGEKIPLDGIVVEGQSFVDTSAITGESLISEVSKDSNVLSGYINKNGV
IKIKVTKTFGESTVSKILELTENASATKANTEKFITKFARYYTPVVVFAALALAVIPTLILKDPDISKWIYRAAVFLVVS
CPCALVISIPLSFFAGIGGASKKGVLIKAGTALEALNDADTIVFDKTGTLTKGVFKVSKIDSEEGVNTEELIEYAAYVES
YSNHPIAKSILKYYEKTIDNKRIEGYEEIVARGVTAYIDGKKVYAGNNKLMEELNINYKKAQEDGVILYIALEDKYIGYI
VINDEIKKDSKETIKSLKDIGIKKAAMLTGDRKSTANNIGTFLGMDEIYSELLPQEKVEKMQSLKSKTSKDGKIVFVGDG
VNDAPVLAMSDIGVSMGGLGSDAAIEASDLVLMSDEPSKLVDAIKIARKTHKIVWQNIIIVLIIKFAVLALAVMGKSTMW
MAVFADVGVALIAVINALRILK

Sequences:

>Translated_742_residues
MIIKKKLLLKGLHCANCATKIERAVQKLNIIEEANYNFNNSTLIINLEETHKDSIIKTIQEIVDRIEPGVKVVDKENLKR
TVVHAPVKNSSNLKMQNNKEENLKLDKKENSYEHNHDHSHGHSHDGEDSDELEKKTLIRIISGVVLLILATVLKSKETLS
IGLYLTSYVLIGGKVILSSIRNISKGQVFDENFLMAVATVAAIGVKQYPEAVAVMLFYEVGEFLQDKAVNKSRKSITALM
NIRPDYANLVRGEDIEVVSPEDINIDDIIMVKPGEKIPLDGIVVEGQSFVDTSAITGESLISEVSKDSNVLSGYINKNGV
IKIKVTKTFGESTVSKILELTENASATKANTEKFITKFARYYTPVVVFAALALAVIPTLILKDPDISKWIYRAAVFLVVS
CPCALVISIPLSFFAGIGGASKKGVLIKAGTALEALNDADTIVFDKTGTLTKGVFKVSKIDSEEGVNTEELIEYAAYVES
YSNHPIAKSILKYYEKTIDNKRIEGYEEIVARGVTAYIDGKKVYAGNNKLMEELNINYKKAQEDGVILYIALEDKYIGYI
VINDEIKKDSKETIKSLKDIGIKKAAMLTGDRKSTANNIGTFLGMDEIYSELLPQEKVEKMQSLKSKTSKDGKIVFVGDG
VNDAPVLAMSDIGVSMGGLGSDAAIEASDLVLMSDEPSKLVDAIKIARKTHKIVWQNIIIVLIIKFAVLALAVMGKSTMW
MAVFADVGVALIAVINALRILK
>Mature_742_residues
MIIKKKLLLKGLHCANCATKIERAVQKLNIIEEANYNFNNSTLIINLEETHKDSIIKTIQEIVDRIEPGVKVVDKENLKR
TVVHAPVKNSSNLKMQNNKEENLKLDKKENSYEHNHDHSHGHSHDGEDSDELEKKTLIRIISGVVLLILATVLKSKETLS
IGLYLTSYVLIGGKVILSSIRNISKGQVFDENFLMAVATVAAIGVKQYPEAVAVMLFYEVGEFLQDKAVNKSRKSITALM
NIRPDYANLVRGEDIEVVSPEDINIDDIIMVKPGEKIPLDGIVVEGQSFVDTSAITGESLISEVSKDSNVLSGYINKNGV
IKIKVTKTFGESTVSKILELTENASATKANTEKFITKFARYYTPVVVFAALALAVIPTLILKDPDISKWIYRAAVFLVVS
CPCALVISIPLSFFAGIGGASKKGVLIKAGTALEALNDADTIVFDKTGTLTKGVFKVSKIDSEEGVNTEELIEYAAYVES
YSNHPIAKSILKYYEKTIDNKRIEGYEEIVARGVTAYIDGKKVYAGNNKLMEELNINYKKAQEDGVILYIALEDKYIGYI
VINDEIKKDSKETIKSLKDIGIKKAAMLTGDRKSTANNIGTFLGMDEIYSELLPQEKVEKMQSLKSKTSKDGKIVFVGDG
VNDAPVLAMSDIGVSMGGLGSDAAIEASDLVLMSDEPSKLVDAIKIARKTHKIVWQNIIIVLIIKFAVLALAVMGKSTMW
MAVFADVGVALIAVINALRILK

Specific function: Couples the hydrolysis of ATP with the transport of cadmium, zinc and cobalt out of the cell. Does not seem to transport copper [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=594, Percent_Identity=27.9461279461279, Blast_Score=232, Evalue=8e-61,
Organism=Homo sapiens, GI55743073, Length=558, Percent_Identity=27.9569892473118, Blast_Score=209, Evalue=7e-54,
Organism=Homo sapiens, GI115529486, Length=319, Percent_Identity=29.4670846394984, Blast_Score=157, Evalue=5e-38,
Organism=Escherichia coli, GI1789879, Length=744, Percent_Identity=29.7043010752688, Blast_Score=305, Evalue=6e-84,
Organism=Escherichia coli, GI1786691, Length=734, Percent_Identity=25.4768392370572, Blast_Score=256, Evalue=4e-69,
Organism=Escherichia coli, GI1786914, Length=546, Percent_Identity=26.007326007326, Blast_Score=134, Evalue=3e-32,
Organism=Escherichia coli, GI2367363, Length=234, Percent_Identity=26.9230769230769, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17556548, Length=577, Percent_Identity=28.9428076256499, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17559224, Length=259, Percent_Identity=27.7992277992278, Blast_Score=66, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71997275, Length=191, Percent_Identity=28.2722513089005, Blast_Score=65, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI71997262, Length=191, Percent_Identity=28.2722513089005, Blast_Score=65, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6320475, Length=546, Percent_Identity=28.9377289377289, Blast_Score=218, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6319772, Length=604, Percent_Identity=27.1523178807947, Blast_Score=175, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6325221, Length=635, Percent_Identity=23.7795275590551, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321430, Length=634, Percent_Identity=22.7129337539432, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321271, Length=218, Percent_Identity=31.651376146789, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321432, Length=185, Percent_Identity=30.8108108108108, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221329854, Length=308, Percent_Identity=30.5194805194805, Blast_Score=137, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24668704, Length=158, Percent_Identity=30.379746835443, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24668708, Length=158, Percent_Identity=30.379746835443, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281366617, Length=158, Percent_Identity=30.379746835443, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI161085803, Length=158, Percent_Identity=30.379746835443, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24668696, Length=158, Percent_Identity=30.379746835443, Blast_Score=70, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR001366
- InterPro:   IPR006404
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: =3.6.3.3; =3.6.3.5 [H]

Molecular weight: Translated: 81447; Mature: 81447

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00154 ATPASE_E1_E2 ; PS50846 HMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKKKLLLKGLHCANCATKIERAVQKLNIIEEANYNFNNSTLIINLEETHKDSIIKTIQ
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHH
EIVDRIEPGVKVVDKENLKRTVVHAPVKNSSNLKMQNNKEENLKLDKKENSYEHNHDHSH
HHHHHHCCCCEEECHHCCHHHEEECCCCCCCCCEECCCCCCCCEECCCCCCCCCCCCCCC
GHSHDGEDSDELEKKTLIRIISGVVLLILATVLKSKETLSIGLYLTSYVLIGGKVILSSI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHH
RNISKGQVFDENFLMAVATVAAIGVKQYPEAVAVMLFYEVGEFLQDKAVNKSRKSITALM
HCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
NIRPDYANLVRGEDIEVVSPEDINIDDIIMVKPGEKIPLDGIVVEGQSFVDTSAITGESL
HCCCCHHHHCCCCCEEEECCCCCCCCCEEEECCCCCCCCCCEEECCCCCCCHHHCCHHHH
ISEVSKDSNVLSGYINKNGVIKIKVTKTFGESTVSKILELTENASATKANTEKFITKFAR
HHHHHCCCCHHHHEECCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
YYTPVVVFAALALAVIPTLILKDPDISKWIYRAAVFLVVSCPCALVISIPLSFFAGIGGA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SKKGVLIKAGTALEALNDADTIVFDKTGTLTKGVFKVSKIDSEEGVNTEELIEYAAYVES
CCCCEEEECCCHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
YSNHPIAKSILKYYEKTIDNKRIEGYEEIVARGVTAYIDGKKVYAGNNKLMEELNINYKK
HCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCEEEECCHHHHHHHCCCCEE
AQEDGVILYIALEDKYIGYIVINDEIKKDSKETIKSLKDIGIKKAAMLTGDRKSTANNIG
CCCCCEEEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHEECCCCHHHHHHHH
TFLGMDEIYSELLPQEKVEKMQSLKSKTSKDGKIVFVGDGVNDAPVLAMSDIGVSMGGLG
HHCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCC
SDAAIEASDLVLMSDEPSKLVDAIKIARKTHKIVWQNIIIVLIIKFAVLALAVMGKSTMW
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
MAVFADVGVALIAVINALRILK
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIIKKKLLLKGLHCANCATKIERAVQKLNIIEEANYNFNNSTLIINLEETHKDSIIKTIQ
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHH
EIVDRIEPGVKVVDKENLKRTVVHAPVKNSSNLKMQNNKEENLKLDKKENSYEHNHDHSH
HHHHHHCCCCEEECHHCCHHHEEECCCCCCCCCEECCCCCCCCEECCCCCCCCCCCCCCC
GHSHDGEDSDELEKKTLIRIISGVVLLILATVLKSKETLSIGLYLTSYVLIGGKVILSSI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHH
RNISKGQVFDENFLMAVATVAAIGVKQYPEAVAVMLFYEVGEFLQDKAVNKSRKSITALM
HCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
NIRPDYANLVRGEDIEVVSPEDINIDDIIMVKPGEKIPLDGIVVEGQSFVDTSAITGESL
HCCCCHHHHCCCCCEEEECCCCCCCCCEEEECCCCCCCCCCEEECCCCCCCHHHCCHHHH
ISEVSKDSNVLSGYINKNGVIKIKVTKTFGESTVSKILELTENASATKANTEKFITKFAR
HHHHHCCCCHHHHEECCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
YYTPVVVFAALALAVIPTLILKDPDISKWIYRAAVFLVVSCPCALVISIPLSFFAGIGGA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SKKGVLIKAGTALEALNDADTIVFDKTGTLTKGVFKVSKIDSEEGVNTEELIEYAAYVES
CCCCEEEECCCHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
YSNHPIAKSILKYYEKTIDNKRIEGYEEIVARGVTAYIDGKKVYAGNNKLMEELNINYKK
HCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCEEEECCHHHHHHHCCCCEE
AQEDGVILYIALEDKYIGYIVINDEIKKDSKETIKSLKDIGIKKAAMLTGDRKSTANNIG
CCCCCEEEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHEECCCCHHHHHHHH
TFLGMDEIYSELLPQEKVEKMQSLKSKTSKDGKIVFVGDGVNDAPVLAMSDIGVSMGGLG
HHCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCC
SDAAIEASDLVLMSDEPSKLVDAIKIARKTHKIVWQNIIIVLIIKFAVLALAVMGKSTMW
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
MAVFADVGVALIAVINALRILK
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]