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The map label for this gene is metK

Identifier: 153936792

GI number: 153936792

Start: 193580

End: 194755

Strand: Direct

Name: metK

Synonym: CLC_0222

Alternate gene names: 153936792

Gene position: 193580-194755 (Clockwise)

Preceding gene: 153935639

Following gene: 153936042

Centisome position: 5.15

GC content: 34.18

Gene sequence:

>1176_bases
GTGAGAAAGTTATTTACATCTGAATCAGTAACAGAAGGGCATCCAGATAAAATATGTGACCAAATTTCAGATGCAGTTTT
AGATGCTATATTAGATAAAGATCCTAATGGAAGGGTTGCTTGCGAAACAGCGGTTACAACAGGAATGGTAATGGTAATGG
GAGAGATATCAACAAAATGTTATGTTGATATACCAAAGTTAGTTAGAGAAACTATAAGGGGAATAGGATATGATAGAGCT
AAATATGGATTTGATTGTGAGACTTGCTCTGTAATAACATCAATAGATGAACAATCAGTAGATATAGCCATGGGTGTTGA
TGAAGCCTTAGAATCTAAAAAAGGTGAAATGGATAAATTAGACGCTGTAGGCGCTGGAGATCAAGGTATGATGTTTGGTT
TTGCTACAAACGAAACTAAGGAATATATGCCAATGCCTATAGAGATGGCCCACAAATTATCAAGAAGATTATCAGAAGTA
AGAAAAAATGGTACATTACCATATTTGAGACCAGATGGAAAAACTCAAGTGACTGTAGAGTATGAGAATGGTAAACCTGT
AAGAATAGATGCTATAGTTATTTCAACTCAACATGGTCCAGAAGTTTATTTAGAGCAAATAGAAAAGGATATTAAAGAAC
ATGTAATAAAAGTTATAGTACCATCAGAGTTATTAGATGAAAATACTAAATATTTTATAAATCCAACAGGAAGATTTGTA
GTTGGTGGACCTCAAGGAGATTCAGGATTAACAGGAAGAAAAATAATAGTTGATACTTATGGTGGATATGGAAGACATGG
TGGTGGAGCCTTTTCAGGTAAGGATCCAACAAAGGTTGATAGGTCTGCTGCATATGCTGCAAGATGGGTAGCTAAAAATT
TAGTTGCAGCAGGGGTTGCTGATAAGCTAGAAATTCAGTTAGCTTATGCTATAGGGGTAGCTAAACCAGTATCTATTTCG
GTTGATACATTTGGAACAGGTAAAATGACTGATGAAGAAATAGTTTCAATAGTAAATAAAGTATTTGATTTAAGACCTGG
TGCTATAATAAGAGATTTAGACTTAAGAAGACCTATATATAAACAAGTTGCTGCCTATGGTCATTTTGGAAGAACAGATA
TAGATGTACCGTGGGAAAGATTAGATAAAGTAGAGGAAATAAAAAAGCATATATAA

Upstream 100 bases:

>100_bases
GTATTATCTGCAAGATAAGAGAGAGTAGTTAGACAATAACCTCTTCTTATTGAAGAGGTTTTATTTTTTAGGCAAAAATT
GGTAATTAGGAGGAGTTAAT

Downstream 100 bases:

>100_bases
ATTAATATTAGTATTAATATGAAAAGGAATGGACATGATAACATGCCATTCCTTTATATTTTTTCATAATAATATGATAT
AATTGTTCTAAATGAATAGC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MRKLFTSESVTEGHPDKICDQISDAVLDAILDKDPNGRVACETAVTTGMVMVMGEISTKCYVDIPKLVRETIRGIGYDRA
KYGFDCETCSVITSIDEQSVDIAMGVDEALESKKGEMDKLDAVGAGDQGMMFGFATNETKEYMPMPIEMAHKLSRRLSEV
RKNGTLPYLRPDGKTQVTVEYENGKPVRIDAIVISTQHGPEVYLEQIEKDIKEHVIKVIVPSELLDENTKYFINPTGRFV
VGGPQGDSGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGVADKLEIQLAYAIGVAKPVSIS
VDTFGTGKMTDEEIVSIVNKVFDLRPGAIIRDLDLRRPIYKQVAAYGHFGRTDIDVPWERLDKVEEIKKHI

Sequences:

>Translated_391_residues
MRKLFTSESVTEGHPDKICDQISDAVLDAILDKDPNGRVACETAVTTGMVMVMGEISTKCYVDIPKLVRETIRGIGYDRA
KYGFDCETCSVITSIDEQSVDIAMGVDEALESKKGEMDKLDAVGAGDQGMMFGFATNETKEYMPMPIEMAHKLSRRLSEV
RKNGTLPYLRPDGKTQVTVEYENGKPVRIDAIVISTQHGPEVYLEQIEKDIKEHVIKVIVPSELLDENTKYFINPTGRFV
VGGPQGDSGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGVADKLEIQLAYAIGVAKPVSIS
VDTFGTGKMTDEEIVSIVNKVFDLRPGAIIRDLDLRRPIYKQVAAYGHFGRTDIDVPWERLDKVEEIKKHI
>Mature_391_residues
MRKLFTSESVTEGHPDKICDQISDAVLDAILDKDPNGRVACETAVTTGMVMVMGEISTKCYVDIPKLVRETIRGIGYDRA
KYGFDCETCSVITSIDEQSVDIAMGVDEALESKKGEMDKLDAVGAGDQGMMFGFATNETKEYMPMPIEMAHKLSRRLSEV
RKNGTLPYLRPDGKTQVTVEYENGKPVRIDAIVISTQHGPEVYLEQIEKDIKEHVIKVIVPSELLDENTKYFINPTGRFV
VGGPQGDSGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGVADKLEIQLAYAIGVAKPVSIS
VDTFGTGKMTDEEIVSIVNKVFDLRPGAIIRDLDLRRPIYKQVAAYGHFGRTDIDVPWERLDKVEEIKKHI

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=58.9473684210526, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=58.9473684210526, Blast_Score=446, Evalue=1e-125,
Organism=Escherichia coli, GI1789311, Length=387, Percent_Identity=61.2403100775194, Blast_Score=486, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17538498, Length=381, Percent_Identity=57.2178477690289, Blast_Score=436, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17551082, Length=381, Percent_Identity=57.742782152231, Blast_Score=436, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI25145633, Length=387, Percent_Identity=57.3643410852713, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17538494, Length=381, Percent_Identity=56.9553805774278, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI32565905, Length=381, Percent_Identity=51.4435695538058, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565911, Length=336, Percent_Identity=54.7619047619048, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25145635, Length=342, Percent_Identity=54.9707602339181, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565903, Length=309, Percent_Identity=54.3689320388349, Blast_Score=329, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI32565909, Length=81, Percent_Identity=62.962962962963, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=38, Percent_Identity=78.9473684210526, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=381, Percent_Identity=58.005249343832, Blast_Score=449, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320710, Length=386, Percent_Identity=56.9948186528497, Blast_Score=446, Evalue=1e-126,
Organism=Drosophila melanogaster, GI45552159, Length=386, Percent_Identity=53.3678756476684, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580529, Length=386, Percent_Identity=53.3678756476684, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580537, Length=386, Percent_Identity=53.3678756476684, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580533, Length=386, Percent_Identity=53.3678756476684, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580535, Length=386, Percent_Identity=53.3678756476684, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580531, Length=386, Percent_Identity=53.3678756476684, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580527, Length=386, Percent_Identity=53.1088082901554, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580525, Length=386, Percent_Identity=53.1088082901554, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580541, Length=374, Percent_Identity=51.8716577540107, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580539, Length=125, Percent_Identity=54.4, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_CLOB1 (A7FQI8)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001382572.1
- STRING:   A7FQI8
- GeneID:   5397301
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0208
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   CBOT441770:CLB_0208-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 43027; Mature: 43027

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLFTSESVTEGHPDKICDQISDAVLDAILDKDPNGRVACETAVTTGMVMVMGEISTKC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHCCEEEEECCCCEE
YVDIPKLVRETIRGIGYDRAKYGFDCETCSVITSIDEQSVDIAMGVDEALESKKGEMDKL
EEECHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHCCCCCCEEEECHHHHHHHCCCCCHHH
DAVGAGDQGMMFGFATNETKEYMPMPIEMAHKLSRRLSEVRKNGTLPYLRPDGKTQVTVE
HCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEE
YENGKPVRIDAIVISTQHGPEVYLEQIEKDIKEHVIKVIVPSELLDENTKYFINPTGRFV
ECCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCEE
VGGPQGDSGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGVA
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DKLEIQLAYAIGVAKPVSISVDTFGTGKMTDEEIVSIVNKVFDLRPGAIIRDLDLRRPIY
CCEEEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHH
KQVAAYGHFGRTDIDVPWERLDKVEEIKKHI
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKLFTSESVTEGHPDKICDQISDAVLDAILDKDPNGRVACETAVTTGMVMVMGEISTKC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHCCEEEEECCCCEE
YVDIPKLVRETIRGIGYDRAKYGFDCETCSVITSIDEQSVDIAMGVDEALESKKGEMDKL
EEECHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHCCCCCCEEEECHHHHHHHCCCCCHHH
DAVGAGDQGMMFGFATNETKEYMPMPIEMAHKLSRRLSEVRKNGTLPYLRPDGKTQVTVE
HCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEE
YENGKPVRIDAIVISTQHGPEVYLEQIEKDIKEHVIKVIVPSELLDENTKYFINPTGRFV
ECCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCEE
VGGPQGDSGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGVA
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DKLEIQLAYAIGVAKPVSISVDTFGTGKMTDEEIVSIVNKVFDLRPGAIIRDLDLRRPIY
CCEEEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHH
KQVAAYGHFGRTDIDVPWERLDKVEEIKKHI
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA