Click here to switch to the map view.

The map label for this gene is prfA

Identifier: 153936703

GI number: 153936703

Start: 169236

End: 170312

Strand: Direct

Name: prfA

Synonym: CLC_0187

Alternate gene names: 153936703

Gene position: 169236-170312 (Clockwise)

Preceding gene: 153935975

Following gene: 153934667

Centisome position: 4.5

GC content: 32.22

Gene sequence:

>1077_bases
ATGTTAGAAAGACTTAATTTTATAGAAAATAAATATGAAGAACTATCAAATAAAATAAGTGACCCTTCAGTTATGGCAAA
TCAAAAAGAATGGCAAAAACTTTGTAAAGAGCATGCTGATTTAGAAATTATAGTTAATACATATAGAGAGTATAAAAAGG
CACAAGAAGATTTAGAATCAGACAAAGAAATGCTAAAGGAAGAATCAGACAAAGAACTAAGAGAAATGGCACAGGAAGAA
ATAAAAGAGTTAACTTTAAAATTAGAAGATTTAGAAAGAGAATTAACTATATTATTACTACCAAAAGATCCTAATGATGA
TAAGGATGTATTTATAGAAATTAGAGCGGGTGCCGGTGGAGAAGAGGCGGCTTTATTTGCTTCTAACTTATTAAGAATGT
ATACAAGATATGCGGAAAGAAAAAATTGGAAAGTAGAAACTATAAGCTTAAATGCTACAGACATAGGTGGTTTTAAAGAA
GTAACTGTAGCGGTTAAAGGAAAAGGTGCTTATAGTAGATTAAAATATGAAAGTGGAGTTCATAGAGTTCAGAGAGTTCC
AGACACAGAATCAAGCGGTAGAATTCATACTTCTACAGCTACTGTAGCAGTACTTCCTGAGGTAGATGATGTAGATATAA
ATATAAATGCTAATGATTTAAGAATAGATGTTTATAGAGCGTCAGGACATGGTGGACAGTGCGTAAATACTACAGATTCT
GCTGTAAGAATAACTCACTTACCGACAGGGCTTGTAGTTACATGCCAAGATGAAAAATCACAATTAAAAAATAAAGAGAA
AGCGATGAAAGTTTTAAAGGCTAGATTGTTTGAAGCTGCAGAGGCTGAAAGAGCGGCATCTATAGCAGAAGATAGAAAAA
GTCAGGTTGGTACAGGAGATAGAAGTGAAAGAATAAGAACTTATAACTATCCTCAAGGAAGAATCACAGATCATAGAATA
GGTTTAACATTATACAAATTAGAAACTTTCTTAGATGGTGATATAGATGAAGCTATAGAAGCTTTAGTAACAGAAGATCA
AGCGGAAAAAATGAAAGATTTAGGTAGAGTTAATTAA

Upstream 100 bases:

>100_bases
AAAAGCTAGAAAGTATTAAAATTCACAGGTTAGTTGTAAATGCTTTATAAAGTATGATATAATATAAAATTGTGAAAATA
TATATACGGAGTGAGAAATA

Downstream 100 bases:

>100_bases
TATTAATTAAGGAAAGAAGAGGTAATTATGGATTTAAACAAAGGCCGTAGAAATCAAAAGAGATCTTATGAAAGATTTGT
TGTGATTATGAGCTTTATTT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MLERLNFIENKYEELSNKISDPSVMANQKEWQKLCKEHADLEIIVNTYREYKKAQEDLESDKEMLKEESDKELREMAQEE
IKELTLKLEDLERELTILLLPKDPNDDKDVFIEIRAGAGGEEAALFASNLLRMYTRYAERKNWKVETISLNATDIGGFKE
VTVAVKGKGAYSRLKYESGVHRVQRVPDTESSGRIHTSTATVAVLPEVDDVDININANDLRIDVYRASGHGGQCVNTTDS
AVRITHLPTGLVVTCQDEKSQLKNKEKAMKVLKARLFEAAEAERAASIAEDRKSQVGTGDRSERIRTYNYPQGRITDHRI
GLTLYKLETFLDGDIDEAIEALVTEDQAEKMKDLGRVN

Sequences:

>Translated_358_residues
MLERLNFIENKYEELSNKISDPSVMANQKEWQKLCKEHADLEIIVNTYREYKKAQEDLESDKEMLKEESDKELREMAQEE
IKELTLKLEDLERELTILLLPKDPNDDKDVFIEIRAGAGGEEAALFASNLLRMYTRYAERKNWKVETISLNATDIGGFKE
VTVAVKGKGAYSRLKYESGVHRVQRVPDTESSGRIHTSTATVAVLPEVDDVDININANDLRIDVYRASGHGGQCVNTTDS
AVRITHLPTGLVVTCQDEKSQLKNKEKAMKVLKARLFEAAEAERAASIAEDRKSQVGTGDRSERIRTYNYPQGRITDHRI
GLTLYKLETFLDGDIDEAIEALVTEDQAEKMKDLGRVN
>Mature_358_residues
MLERLNFIENKYEELSNKISDPSVMANQKEWQKLCKEHADLEIIVNTYREYKKAQEDLESDKEMLKEESDKELREMAQEE
IKELTLKLEDLERELTILLLPKDPNDDKDVFIEIRAGAGGEEAALFASNLLRMYTRYAERKNWKVETISLNATDIGGFKE
VTVAVKGKGAYSRLKYESGVHRVQRVPDTESSGRIHTSTATVAVLPEVDDVDININANDLRIDVYRASGHGGQCVNTTDS
AVRITHLPTGLVVTCQDEKSQLKNKEKAMKVLKARLFEAAEAERAASIAEDRKSQVGTGDRSERIRTYNYPQGRITDHRI
GLTLYKLETFLDGDIDEAIEALVTEDQAEKMKDLGRVN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=284, Percent_Identity=48.2394366197183, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI34577120, Length=341, Percent_Identity=41.0557184750733, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI166795305, Length=189, Percent_Identity=46.5608465608466, Blast_Score=193, Evalue=2e-49,
Organism=Escherichia coli, GI1787462, Length=355, Percent_Identity=50.9859154929577, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=41.9161676646707, Blast_Score=235, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI17542784, Length=286, Percent_Identity=38.1118881118881, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=329, Percent_Identity=46.2006079027356, Blast_Score=278, Evalue=1e-75,
Organism=Drosophila melanogaster, GI19921226, Length=338, Percent_Identity=44.6745562130178, Blast_Score=256, Evalue=1e-68,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_CLOB1 (A7FQG2)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001382546.1
- ProteinModelPortal:   A7FQG2
- SMR:   A7FQG2
- STRING:   A7FQG2
- GeneID:   5395372
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0175
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   GEAGGYK
- ProtClustDB:   PRK00591
- BioCyc:   CBOT441770:CLB_0175-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40599; Mature: 40599

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERLNFIENKYEELSNKISDPSVMANQKEWQKLCKEHADLEIIVNTYREYKKAQEDLES
CCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHH
DKEMLKEESDKELREMAQEEIKELTLKLEDLERELTILLLPKDPNDDKDVFIEIRAGAGG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEECCCCC
EEAALFASNLLRMYTRYAERKNWKVETISLNATDIGGFKEVTVAVKGKGAYSRLKYESGV
CHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEEECCCHHHHHHHHHHH
HRVQRVPDTESSGRIHTSTATVAVLPEVDDVDININANDLRIDVYRASGHGGQCVNTTDS
HHHHCCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCEEEEEEEEECCCCCCEECCCCC
AVRITHLPTGLVVTCQDEKSQLKNKEKAMKVLKARLFEAAEAERAASIAEDRKSQVGTGD
EEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSERIRTYNYPQGRITDHRIGLTLYKLETFLDGDIDEAIEALVTEDQAEKMKDLGRVN
CCCCCEECCCCCCCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCCC
>Mature Secondary Structure
MLERLNFIENKYEELSNKISDPSVMANQKEWQKLCKEHADLEIIVNTYREYKKAQEDLES
CCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHH
DKEMLKEESDKELREMAQEEIKELTLKLEDLERELTILLLPKDPNDDKDVFIEIRAGAGG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEECCCCC
EEAALFASNLLRMYTRYAERKNWKVETISLNATDIGGFKEVTVAVKGKGAYSRLKYESGV
CHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEEECCCHHHHHHHHHHH
HRVQRVPDTESSGRIHTSTATVAVLPEVDDVDININANDLRIDVYRASGHGGQCVNTTDS
HHHHCCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCEEEEEEEEECCCCCCEECCCCC
AVRITHLPTGLVVTCQDEKSQLKNKEKAMKVLKARLFEAAEAERAASIAEDRKSQVGTGD
EEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSERIRTYNYPQGRITDHRIGLTLYKLETFLDGDIDEAIEALVTEDQAEKMKDLGRVN
CCCCCEECCCCCCCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA