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The map label for this gene is glnA [H]

Identifier: 153936517

GI number: 153936517

Start: 3681996

End: 3683894

Strand: Direct

Name: glnA [H]

Synonym: CLC_3543

Alternate gene names: 153936517

Gene position: 3681996-3683894 (Clockwise)

Preceding gene: 153935210

Following gene: 153935056

Centisome position: 97.91

GC content: 30.02

Gene sequence:

>1899_bases
ATGTTAGATGATTTAGTATATGTTATTCCTAAAGAAATGCATTCCGAAGAGCAACTTAAAGAATTTTTAGTTTCCCATCC
TGAAATAAAATTTGTGTCTTTAGTTTGTATCGATTTGGCTGGTAATGATACTGACGAAAAAATTCCTATAAAATTATTTT
TAGAAGACATGAGTAAATTCATAAAAGGCTGTGTTCAAACAGACGGATCCTCTGTTGTCCTTCCAGGTATTGCAACTCTT
AATAATGCTAAAGTTGATATGATTGTGGACATGGATGTTAACTGGTTTATAGATTATAACTATGAACTTATAGACTGTAA
AACCGAAAAACCAACCGGTACTCTTAGAATTCCTTGCTTTCTTTACCACGAAAATAAACCCGTGGATTCCAGATATATAT
TAAAAAAATCAATAGAATATTTTAAAACAACTCTATTAAATTTAATAAAGACTAACCCTGAATCTATATCTTCTTTCAAT
ATAAAATATGAAGATATAGAAAAAGTAGATGTAACTTGTGCTACAGAACTAGAATTTTGGGTGAAAACTCCAAATGACAA
TGCAGAAGTAGATGAATTGTGTACTTCTCAAGTTCTTCATGAACAATACTGGAATAGAACCAAAGGAAGCGTTAGAACTG
CTTTAGAGGAATCTTTATTACTTATGGAATATCACGGATTAGAGCCTGAAATGGGTCATAAAGAAGTTGGTGGAGTAAAA
GCTAAATTAACTAGTTCCGGTGGATTTACTCATATAATGGAACAGCTAGAAGTTGATTGGAAATATTCAACAGCTCTTCA
AGCAGGAGATAATGAATTATTAGTAAAAAGCATAATAAAAGAAACCTTTAGAAATAACAAATTAGATGTTACATTTTTTG
CAAAACCTATAGATGAAGTTGCAGGCAGCGGAAAACATGTGCATTTAGGGGTTGCGCTTAAATTAAAAAATGGAAAAAGA
ATTAATCTTTTCTCTACAGATAAAAACCACTACCTAAGTTCTTTTGGTTATGCTTCTATAATGGGCATACTAAAAAATTA
TGAAGTGATAAATCCTTTTGTTTCATCAACTAACGATTCTTTAAAAAGGCTGAAACCAGGCTTTGAAGCACCTGTATGTA
TAGTAACATCTTTAGGCCATACTGTACAATTACCATCAAGAAATAGAACAATCCTAGTTGGGGTTATAAGAGACTTAGAT
AATTCTTTAGCTACAAGATTTGAGTTAAGATCACCAAATCCTCGTAGTAATACCTATCTAACAGTAGCTTCTTTATGTAT
GTCTATGATTGATGGAATAAAATATGCTATAGAAAACAATAAATGTGAAGATGATCTTTTAAAAGAGCTTTCTAAAGCTG
CTGAGGAAGATACTAGCTATCTTGAAAAGGGAAGACAATATAGAAGTGAAGAAGACGTATTTGAATACTACAGTGAAGAA
GAAAGAAGTAAAATCTTTGGCACAGCACCTTCTACTGTATATGAAAATATATGCTCTTTAGATAAATATATGGATAAGAT
AGAAGTATTAAAAGCTGGTGAGGTATTCACCTCTAAAATTGTTAATAGCTATAAACTAGCTGTTATTGAAAGATGGTTAG
TTGAAATAACAAATAGAATAATTCCAAATTATGTAGAAGAAATCAGAAGCTTTTCTCAATTACATGAAGTAAACAAAGCC
TCTGATTTAGATATTCATAACTGGTCAAAAATCAATGAGCTTAGACAATGTCTAATGAAAGATACCTACTCTGATAAATC
TTTATTTGGACAAATAAGAGAAGCTAAAGAAAATAACGATTATGAAACTATTTCTAATTTACAATTAGAAATAGATAAAA
AAATGAAATTACTTAGAAAACTTTATTCCGATTATAAAAAAAATCTTTTAGATATATAA

Upstream 100 bases:

>100_bases
GACATATAAAAATTTTAATTATAATATATTGCATATATTATATACAATAAAACTATATAATGTTAAATTGATTGTATTTA
AAAATTTGGGGAGGTAAATT

Downstream 100 bases:

>100_bases
AATAAAAGCACACCTAATATCAGGTGTGCTTTTATCCTATAATATTAAAATTCTTTTAATGGAAAAGACTTTATTACCAT
TTCCTTTTTATTATTTATAG

Product: putative glutamine synthetase

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MLDDLVYVIPKEMHSEEQLKEFLVSHPEIKFVSLVCIDLAGNDTDEKIPIKLFLEDMSKFIKGCVQTDGSSVVLPGIATL
NNAKVDMIVDMDVNWFIDYNYELIDCKTEKPTGTLRIPCFLYHENKPVDSRYILKKSIEYFKTTLLNLIKTNPESISSFN
IKYEDIEKVDVTCATELEFWVKTPNDNAEVDELCTSQVLHEQYWNRTKGSVRTALEESLLLMEYHGLEPEMGHKEVGGVK
AKLTSSGGFTHIMEQLEVDWKYSTALQAGDNELLVKSIIKETFRNNKLDVTFFAKPIDEVAGSGKHVHLGVALKLKNGKR
INLFSTDKNHYLSSFGYASIMGILKNYEVINPFVSSTNDSLKRLKPGFEAPVCIVTSLGHTVQLPSRNRTILVGVIRDLD
NSLATRFELRSPNPRSNTYLTVASLCMSMIDGIKYAIENNKCEDDLLKELSKAAEEDTSYLEKGRQYRSEEDVFEYYSEE
ERSKIFGTAPSTVYENICSLDKYMDKIEVLKAGEVFTSKIVNSYKLAVIERWLVEITNRIIPNYVEEIRSFSQLHEVNKA
SDLDIHNWSKINELRQCLMKDTYSDKSLFGQIREAKENNDYETISNLQLEIDKKMKLLRKLYSDYKKNLLDI

Sequences:

>Translated_632_residues
MLDDLVYVIPKEMHSEEQLKEFLVSHPEIKFVSLVCIDLAGNDTDEKIPIKLFLEDMSKFIKGCVQTDGSSVVLPGIATL
NNAKVDMIVDMDVNWFIDYNYELIDCKTEKPTGTLRIPCFLYHENKPVDSRYILKKSIEYFKTTLLNLIKTNPESISSFN
IKYEDIEKVDVTCATELEFWVKTPNDNAEVDELCTSQVLHEQYWNRTKGSVRTALEESLLLMEYHGLEPEMGHKEVGGVK
AKLTSSGGFTHIMEQLEVDWKYSTALQAGDNELLVKSIIKETFRNNKLDVTFFAKPIDEVAGSGKHVHLGVALKLKNGKR
INLFSTDKNHYLSSFGYASIMGILKNYEVINPFVSSTNDSLKRLKPGFEAPVCIVTSLGHTVQLPSRNRTILVGVIRDLD
NSLATRFELRSPNPRSNTYLTVASLCMSMIDGIKYAIENNKCEDDLLKELSKAAEEDTSYLEKGRQYRSEEDVFEYYSEE
ERSKIFGTAPSTVYENICSLDKYMDKIEVLKAGEVFTSKIVNSYKLAVIERWLVEITNRIIPNYVEEIRSFSQLHEVNKA
SDLDIHNWSKINELRQCLMKDTYSDKSLFGQIREAKENNDYETISNLQLEIDKKMKLLRKLYSDYKKNLLDI
>Mature_632_residues
MLDDLVYVIPKEMHSEEQLKEFLVSHPEIKFVSLVCIDLAGNDTDEKIPIKLFLEDMSKFIKGCVQTDGSSVVLPGIATL
NNAKVDMIVDMDVNWFIDYNYELIDCKTEKPTGTLRIPCFLYHENKPVDSRYILKKSIEYFKTTLLNLIKTNPESISSFN
IKYEDIEKVDVTCATELEFWVKTPNDNAEVDELCTSQVLHEQYWNRTKGSVRTALEESLLLMEYHGLEPEMGHKEVGGVK
AKLTSSGGFTHIMEQLEVDWKYSTALQAGDNELLVKSIIKETFRNNKLDVTFFAKPIDEVAGSGKHVHLGVALKLKNGKR
INLFSTDKNHYLSSFGYASIMGILKNYEVINPFVSSTNDSLKRLKPGFEAPVCIVTSLGHTVQLPSRNRTILVGVIRDLD
NSLATRFELRSPNPRSNTYLTVASLCMSMIDGIKYAIENNKCEDDLLKELSKAAEEDTSYLEKGRQYRSEEDVFEYYSEE
ERSKIFGTAPSTVYENICSLDKYMDKIEVLKAGEVFTSKIVNSYKLAVIERWLVEITNRIIPNYVEEIRSFSQLHEVNKA
SDLDIHNWSKINELRQCLMKDTYSDKSLFGQIREAKENNDYETISNLQLEIDKKMKLLRKLYSDYKKNLLDI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=527, Percent_Identity=26.7552182163188, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 72423; Mature: 72423

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDDLVYVIPKEMHSEEQLKEFLVSHPEIKFVSLVCIDLAGNDTDEKIPIKLFLEDMSKF
CCCCCEEECCHHCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHH
IKGCVQTDGSSVVLPGIATLNNAKVDMIVDMDVNWFIDYNYELIDCKTEKPTGTLRIPCF
HHHHHCCCCCEEEECCCEECCCCCEEEEEECCCEEEEECCEEEEEEECCCCCCEEEEEEE
LYHENKPVDSRYILKKSIEYFKTTLLNLIKTNPESISSFNIKYEDIEKVDVTCATELEFW
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHCHHHCCEEEEEEEEEE
VKTPNDNAEVDELCTSQVLHEQYWNRTKGSVRTALEESLLLMEYHGLEPEMGHKEVGGVK
EECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEE
AKLTSSGGFTHIMEQLEVDWKYSTALQAGDNELLVKSIIKETFRNNKLDVTFFAKPIDEV
EEEECCCCHHHHHHHHHCCCEEHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHH
AGSGKHVHLGVALKLKNGKRINLFSTDKNHYLSSFGYASIMGILKNYEVINPFVSSTNDS
CCCCCEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHH
LKRLKPGFEAPVCIVTSLGHTVQLPSRNRTILVGVIRDLDNSLATRFELRSPNPRSNTYL
HHHCCCCCCCCEEEEECCCCEEECCCCCCEEEEEEHHHHHHHHHHEEEECCCCCCCCCCH
TVASLCMSMIDGIKYAIENNKCEDDLLKELSKAAEEDTSYLEKGRQYRSEEDVFEYYSEE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHH
ERSKIFGTAPSTVYENICSLDKYMDKIEVLKAGEVFTSKIVNSYKLAVIERWLVEITNRI
HHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPNYVEEIRSFSQLHEVNKASDLDIHNWSKINELRQCLMKDTYSDKSLFGQIREAKENND
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
YETISNLQLEIDKKMKLLRKLYSDYKKNLLDI
HHHHHHHHEEHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDDLVYVIPKEMHSEEQLKEFLVSHPEIKFVSLVCIDLAGNDTDEKIPIKLFLEDMSKF
CCCCCEEECCHHCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHH
IKGCVQTDGSSVVLPGIATLNNAKVDMIVDMDVNWFIDYNYELIDCKTEKPTGTLRIPCF
HHHHHCCCCCEEEECCCEECCCCCEEEEEECCCEEEEECCEEEEEEECCCCCCEEEEEEE
LYHENKPVDSRYILKKSIEYFKTTLLNLIKTNPESISSFNIKYEDIEKVDVTCATELEFW
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHCHHHCCEEEEEEEEEE
VKTPNDNAEVDELCTSQVLHEQYWNRTKGSVRTALEESLLLMEYHGLEPEMGHKEVGGVK
EECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEE
AKLTSSGGFTHIMEQLEVDWKYSTALQAGDNELLVKSIIKETFRNNKLDVTFFAKPIDEV
EEEECCCCHHHHHHHHHCCCEEHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHH
AGSGKHVHLGVALKLKNGKRINLFSTDKNHYLSSFGYASIMGILKNYEVINPFVSSTNDS
CCCCCEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHH
LKRLKPGFEAPVCIVTSLGHTVQLPSRNRTILVGVIRDLDNSLATRFELRSPNPRSNTYL
HHHCCCCCCCCEEEEECCCCEEECCCCCCEEEEEEHHHHHHHHHHEEEECCCCCCCCCCH
TVASLCMSMIDGIKYAIENNKCEDDLLKELSKAAEEDTSYLEKGRQYRSEEDVFEYYSEE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHH
ERSKIFGTAPSTVYENICSLDKYMDKIEVLKAGEVFTSKIVNSYKLAVIERWLVEITNRI
HHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPNYVEEIRSFSQLHEVNKASDLDIHNWSKINELRQCLMKDTYSDKSLFGQIREAKENND
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
YETISNLQLEIDKKMKLLRKLYSDYKKNLLDI
HHHHHHHHEEHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]