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The map label for this gene is ispD

Identifier: 153936440

GI number: 153936440

Start: 3606175

End: 3606864

Strand: Reverse

Name: ispD

Synonym: CLC_3453

Alternate gene names: 153936440

Gene position: 3606864-3606175 (Counterclockwise)

Preceding gene: 153937424

Following gene: 153935851

Centisome position: 95.91

GC content: 28.26

Gene sequence:

>690_bases
ATGAGTAAAAATTCTGTTATTATAGTTGCTGCCGGAAAAGGTAAAAGAATGAATAGCAGTATAAGTAAGCAGTTTTTGCA
GATAAAAAACAAACCTATTCTTTATTATACTTTAAGCAAATTTTCAACCCATGAATCTATAGATGAAATAGTGCTAGTTA
CCTTAGAAGATAAAATAGAAGTTTGCAGTGATATTATAGATAAATATAATATCAATAAGGTATCTAAAATAGTTCCAGGA
GGTAAGGAAAGACAAGATTCTGTTTATAATGGACTAAAGGGTGTATCAAAGGATTGTGAGGTAGTATTAATTCATGATGC
AGCAAGACCTTTTGTTACTAGTGATATTATAGAGAATGGTATAAGATATACGAATCAATATGGGGCTGCTGCTTGTGGGG
TAATTCCAAAAGATACCATAAAAATAAAAAATGAAAAAGGATTTGCAATAGATACTCCTAACAGAGAGGATCTATTTATA
GTTCAAACTCCCCAGTGTTTTAATTACAATATAATATTAAGTTGTCATGAAAAGTTAAAAAAACATAATAAAAAAGTAAC
AGATGATACTATGGTTTTAGAGAATTACGGTAAATCAGTATATTTATATGAAGGAAGCTATAGTAATATAAAAATAACAA
CTCCTGAAGATTTAATATTAGGAGAACAAATATTAGAAAAGCTAACTTAG

Upstream 100 bases:

>100_bases
AAAAAACGTATAGGGCAAAATTTAGACGTAGTAGTAACCTCTGTTTTACAAACAGCGGCAGGAAGAATGATTTTTGCTAA
GAGAAAAGGGGAGTCTTAGC

Downstream 100 bases:

>100_bases
CAATAAAACTCGTCCTAATAAGGGGCGAGTTTTGTTTATATATTGCATGTTTAAAATTTCTATCGAGAATATTTTTAAAT
ATAATGAATAGTTTGTTAGG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MSKNSVIIVAAGKGKRMNSSISKQFLQIKNKPILYYTLSKFSTHESIDEIVLVTLEDKIEVCSDIIDKYNINKVSKIVPG
GKERQDSVYNGLKGVSKDCEVVLIHDAARPFVTSDIIENGIRYTNQYGAAACGVIPKDTIKIKNEKGFAIDTPNREDLFI
VQTPQCFNYNIILSCHEKLKKHNKKVTDDTMVLENYGKSVYLYEGSYSNIKITTPEDLILGEQILEKLT

Sequences:

>Translated_229_residues
MSKNSVIIVAAGKGKRMNSSISKQFLQIKNKPILYYTLSKFSTHESIDEIVLVTLEDKIEVCSDIIDKYNINKVSKIVPG
GKERQDSVYNGLKGVSKDCEVVLIHDAARPFVTSDIIENGIRYTNQYGAAACGVIPKDTIKIKNEKGFAIDTPNREDLFI
VQTPQCFNYNIILSCHEKLKKHNKKVTDDTMVLENYGKSVYLYEGSYSNIKITTPEDLILGEQILEKLT
>Mature_228_residues
SKNSVIIVAAGKGKRMNSSISKQFLQIKNKPILYYTLSKFSTHESIDEIVLVTLEDKIEVCSDIIDKYNINKVSKIVPGG
KERQDSVYNGLKGVSKDCEVVLIHDAARPFVTSDIIENGIRYTNQYGAAACGVIPKDTIKIKNEKGFAIDTPNREDLFIV
QTPQCFNYNIILSCHEKLKKHNKKVTDDTMVLENYGKSVYLYEGSYSNIKITTPEDLILGEQILEKLT

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=233, Percent_Identity=27.4678111587983, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI157671913, Length=134, Percent_Identity=32.8358208955224, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1789104, Length=225, Percent_Identity=31.5555555555556, Blast_Score=104, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_CLOB1 (A7FZ94)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385821.1
- ProteinModelPortal:   A7FZ94
- STRING:   A7FZ94
- GeneID:   5396218
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3564
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   TGQMSIF
- ProtClustDB:   PRK00155
- BioCyc:   CBOT441770:CLB_3564-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25760; Mature: 25629

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNSVIIVAAGKGKRMNSSISKQFLQIKNKPILYYTLSKFSTHESIDEIVLVTLEDKIE
CCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEEHHHHH
VCSDIIDKYNINKVSKIVPGGKERQDSVYNGLKGVSKDCEVVLIHDAARPFVTSDIIENG
HHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHCC
IRYTNQYGAAACGVIPKDTIKIKNEKGFAIDTPNREDLFIVQTPQCFNYNIILSCHEKLK
HHCCCCCCCEECCCCCCCEEEEECCCCCEEECCCCCCEEEEECCCCCCEEEEEEHHHHHH
KHNKKVTDDTMVLENYGKSVYLYEGSYSNIKITTPEDLILGEQILEKLT
HCCCCCCCHHHHHHHCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKNSVIIVAAGKGKRMNSSISKQFLQIKNKPILYYTLSKFSTHESIDEIVLVTLEDKIE
CCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEEHHHHH
VCSDIIDKYNINKVSKIVPGGKERQDSVYNGLKGVSKDCEVVLIHDAARPFVTSDIIENG
HHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHCC
IRYTNQYGAAACGVIPKDTIKIKNEKGFAIDTPNREDLFIVQTPQCFNYNIILSCHEKLK
HHCCCCCCCEECCCCCCCEEEEECCCCCEEECCCCCCEEEEECCCCCCEEEEEEHHHHHH
KHNKKVTDDTMVLENYGKSVYLYEGSYSNIKITTPEDLILGEQILEKLT
HCCCCCCCHHHHHHHCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA