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The map label for this gene is rlmH

Identifier: 153936438

GI number: 153936438

Start: 3688230

End: 3688709

Strand: Direct

Name: rlmH

Synonym: CLC_3549

Alternate gene names: 153936438

Gene position: 3688230-3688709 (Clockwise)

Preceding gene: 153935921

Following gene: 153937187

Centisome position: 98.08

GC content: 30.0

Gene sequence:

>480_bases
ATGAATATATCTATAATTTCAGTAGGGAAAATAAAAGAAAAGTTTTTAAAGGCAGCAATAGACGAGTACTCTAAAAGATT
AAGTAAATACTGTAAACTAAATATAATAGAAGTGGCCGATGAAAAAACTCCAGATAATGCCTCTCTTAAAGAAGAAAATA
TAATAAAAGAAAAAGAAGGTAATTTAATATTAAAACATATAAAAGATAATAGCTTTGTTATAGCTTTAGATTTAAAAGGT
AAATCTATAACCTCAGAAGAATTCTCGGACCTTATAGAAAATTGCAGATTAACAGGTAATAGCACTATTGCCTTTGTTAT
CGGTGGATCCCTAGGATTATCTCAGCAGGTTTTATCCAGAGCTAATTATAAGCTATCTTTCTCAAAAATGACCTTCCCAC
ATCAGCTCTTTAGAGTAATGCTTCTAGAACAAGTTTATAGGGCTTTTAGAATATTATGCAGAGAACCGTACCACAAATAA

Upstream 100 bases:

>100_bases
TTAAATAAAATTTATTTATTGGTAGGCCTTTTAGAGCTTTTCTAAATACTTAAACTCTTTAATAAAAACATCTTGAATTA
CTCTAAACTAGGAGGATAAA

Downstream 100 bases:

>100_bases
AGGACAAAAAGTTGAAGATAGAGAAATAGGGCGAAATAATTAATAAGGTAGAACTTATTGTAGTAATGTAAGTCCTACCT
TATGATTTTATATTCTAGAT

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNISIISVGKIKEKFLKAAIDEYSKRLSKYCKLNIIEVADEKTPDNASLKEENIIKEKEGNLILKHIKDNSFVIALDLKG
KSITSEEFSDLIENCRLTGNSTIAFVIGGSLGLSQQVLSRANYKLSFSKMTFPHQLFRVMLLEQVYRAFRILCREPYHK

Sequences:

>Translated_159_residues
MNISIISVGKIKEKFLKAAIDEYSKRLSKYCKLNIIEVADEKTPDNASLKEENIIKEKEGNLILKHIKDNSFVIALDLKG
KSITSEEFSDLIENCRLTGNSTIAFVIGGSLGLSQQVLSRANYKLSFSKMTFPHQLFRVMLLEQVYRAFRILCREPYHK
>Mature_159_residues
MNISIISVGKIKEKFLKAAIDEYSKRLSKYCKLNIIEVADEKTPDNASLKEENIIKEKEGNLILKHIKDNSFVIALDLKG
KSITSEEFSDLIENCRLTGNSTIAFVIGGSLGLSQQVLSRANYKLSFSKMTFPHQLFRVMLLEQVYRAFRILCREPYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=30.8176100628931, Blast_Score=91, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_CLOB1 (A7FZD1)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385884.1
- ProteinModelPortal:   A7FZD1
- SMR:   A7FZD1
- STRING:   A7FZD1
- GeneID:   5398177
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3651
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- BioCyc:   CBOT441770:CLB_3651-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 18160; Mature: 18160

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISIISVGKIKEKFLKAAIDEYSKRLSKYCKLNIIEVADEKTPDNASLKEENIIKEKEG
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHCCC
NLILKHIKDNSFVIALDLKGKSITSEEFSDLIENCRLTGNSTIAFVIGGSLGLSQQVLSR
CEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHH
ANYKLSFSKMTFPHQLFRVMLLEQVYRAFRILCREPYHK
CCCEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNISIISVGKIKEKFLKAAIDEYSKRLSKYCKLNIIEVADEKTPDNASLKEENIIKEKEG
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHCCC
NLILKHIKDNSFVIALDLKGKSITSEEFSDLIENCRLTGNSTIAFVIGGSLGLSQQVLSR
CEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHH
ANYKLSFSKMTFPHQLFRVMLLEQVYRAFRILCREPYHK
CCCEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA