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The map label for this gene is 153936376

Identifier: 153936376

GI number: 153936376

Start: 1516350

End: 1517177

Strand: Direct

Name: 153936376

Synonym: CLC_1461

Alternate gene names: NA

Gene position: 1516350-1517177 (Clockwise)

Preceding gene: 153936897

Following gene: 153937576

Centisome position: 40.32

GC content: 31.16

Gene sequence:

>828_bases
ATGAAAATAGGAATAGATTGTGGTCATACATTAAGTGGTGTTGATTATGGAGCAGTAGGAATAAAAGCAGAATCTAATTT
AACTAGAGAAGTAGGAACAAAAGTAATAAGTAAATTACAAGACTTAGGGCATACAGTTGTTAAATGTTATAAGGATAGTT
GTAGTAGCTTGAACGATAGTTTAAGCTATAGAGCTAATACAGCTAATAGCAAAAATGTAGATTTATATGTAAGCATACAT
TTTAATTGCTTTAATGGTAATGCACATGGAACAGAGGTGCTAACTTATGGAGAAAAATCTTTTACAGAAGCATCAAGAGT
ATTAAATAATATTTGTGCTTTAGGTTATACAAATAGAGGTATAAAAGACGGTTCTGGTCTTTATGTATTGAAACATACAA
AAGCTAAAGCTATGATTATAGAATGCTGTTTCTGTGATAATAGAAACGATATGAACAGATATAATGCCGAAAATATGGCT
AATGCTATAGTTAAAGGTTTAGTAGGCAAAACTACTTGTACAACATCAAATAGCAAACCAAGCACATCTAAGCCAGTACA
AACTAATAAGAAACATACATTAATAAATCAACTTTATGCGGAAATGTCTAGACAGGGTTTTAATACTTTTCCTGCTTGTA
GGCAGGGTGCTAGAGGCAATATTACAAAGATTATACAACAAATGCTTATCAATATAGGGTATACAGTAGGTTCTTTTGGC
AATAGTACAGTTATGGCAGTCAAATCTTTTCAAAGAGATTGTAACTTATCTGCGGATGTACTTGTAGGAAAAGAAACATG
GAAAGCTTTATTTAGAAATTTAAAATAG

Upstream 100 bases:

>100_bases
TACTAGCAATTAAAAATATGTTAAATACAGAATTTATAACTATTTATACTATGGTTATAGCATTTTATTTTAGTAAAGAT
AGAAAGGAAGTAGATAAATA

Downstream 100 bases:

>100_bases
ATTAAAAGAAGGTAGTTCCTTAATTGAAGCTACCTTTTTTCTTATTGCTAAAGTATAAGTTTTGAATTTATAGTATATAT
TAATTATGCTCAATTATAAA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKIGIDCGHTLSGVDYGAVGIKAESNLTREVGTKVISKLQDLGHTVVKCYKDSCSSLNDSLSYRANTANSKNVDLYVSIH
FNCFNGNAHGTEVLTYGEKSFTEASRVLNNICALGYTNRGIKDGSGLYVLKHTKAKAMIIECCFCDNRNDMNRYNAENMA
NAIVKGLVGKTTCTTSNSKPSTSKPVQTNKKHTLINQLYAEMSRQGFNTFPACRQGARGNITKIIQQMLINIGYTVGSFG
NSTVMAVKSFQRDCNLSADVLVGKETWKALFRNLK

Sequences:

>Translated_275_residues
MKIGIDCGHTLSGVDYGAVGIKAESNLTREVGTKVISKLQDLGHTVVKCYKDSCSSLNDSLSYRANTANSKNVDLYVSIH
FNCFNGNAHGTEVLTYGEKSFTEASRVLNNICALGYTNRGIKDGSGLYVLKHTKAKAMIIECCFCDNRNDMNRYNAENMA
NAIVKGLVGKTTCTTSNSKPSTSKPVQTNKKHTLINQLYAEMSRQGFNTFPACRQGARGNITKIIQQMLINIGYTVGSFG
NSTVMAVKSFQRDCNLSADVLVGKETWKALFRNLK
>Mature_275_residues
MKIGIDCGHTLSGVDYGAVGIKAESNLTREVGTKVISKLQDLGHTVVKCYKDSCSSLNDSLSYRANTANSKNVDLYVSIH
FNCFNGNAHGTEVLTYGEKSFTEASRVLNNICALGYTNRGIKDGSGLYVLKHTKAKAMIIECCFCDNRNDMNRYNAENMA
NAIVKGLVGKTTCTTSNSKPSTSKPVQTNKKHTLINQLYAEMSRQGFNTFPACRQGARGNITKIIQQMLINIGYTVGSFG
NSTVMAVKSFQRDCNLSADVLVGKETWKALFRNLK

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: To C.perfringens pIP404 ORF10 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 30103; Mature: 30103

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIDCGHTLSGVDYGAVGIKAESNLTREVGTKVISKLQDLGHTVVKCYKDSCSSLNDS
CEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSYRANTANSKNVDLYVSIHFNCFNGNAHGTEVLTYGEKSFTEASRVLNNICALGYTNRG
HHCCCCCCCCCCEEEEEEEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCC
IKDGSGLYVLKHTKAKAMIIECCFCDNRNDMNRYNAENMANAIVKGLVGKTTCTTSNSKP
CCCCCCEEEEEECCCCEEEEEEEECCCCCCCHHCCHHHHHHHHHHHHCCCCEECCCCCCC
STSKPVQTNKKHTLINQLYAEMSRQGFNTFPACRQGARGNITKIIQQMLINIGYTVGSFG
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCC
NSTVMAVKSFQRDCNLSADVLVGKETWKALFRNLK
CCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHCCC
>Mature Secondary Structure
MKIGIDCGHTLSGVDYGAVGIKAESNLTREVGTKVISKLQDLGHTVVKCYKDSCSSLNDS
CEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSYRANTANSKNVDLYVSIHFNCFNGNAHGTEVLTYGEKSFTEASRVLNNICALGYTNRG
HHCCCCCCCCCCEEEEEEEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCC
IKDGSGLYVLKHTKAKAMIIECCFCDNRNDMNRYNAENMANAIVKGLVGKTTCTTSNSKP
CCCCCCEEEEEECCCCEEEEEEEECCCCCCCHHCCHHHHHHHHHHHHCCCCEECCCCCCC
STSKPVQTNKKHTLINQLYAEMSRQGFNTFPACRQGARGNITKIIQQMLINIGYTVGSFG
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCC
NSTVMAVKSFQRDCNLSADVLVGKETWKALFRNLK
CCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8052128; 11792842 [H]